• Journal of the Korean Society of Food Culture
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• v.23 no.2
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• pp.273-292
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• 2008

Among all the ingredients usedin mandu, the following types were used:, 13 types of grains were used (12.38%), 30 types of vegetables, fruits, bulbs,and nuts were used (28.57%), 32 types of marine products, birds, meats, fishes, and shellfishes were used (30.48%), 10 types of functional ingredients were used (9.52%) and. For spices, 20 types of spices were used (19.05%). 2. Cooking Methods offor Mandu. The mMandu eaten at in the early Joseon era had was primarily made ofusedbuckwheat that contained boiled tofu or egg uiijuk in the kneaded dough for the most part and while kneading with buckwheat, the tofu or egg uiijuk has been boiled down to knead the dough, and and starch powder, bean powder, or rice powder, etc were mixed to make the mandu coating. Buckwheat powder was mixed toadded to the flourwer or was used by itself, while meat, vegetables, tofu, and shiitake mushroom, etc were also addedincluded. From the 18th century, the host plant, or cabbage kimchi, were prepared and combined had been sliced to be used as filling together while red pepper powder was mixed combined withto spices or vinegar soy sauce to be used together. Also, Radishes had beenwere also used as filling, but shown as not being used fromafter the start of the 1900's. For the shape of mMandu, it was madeinto different shapes such as as triangle, rectangle, date plum, gwebul, half moon, or pomegranate shapes, and then shapes to be boiled in simmering water, baked, or cooked as soup in clear broth for soup., In the 17th to 18th century, boilingthen in a steamer gradually became a cooking style, assumed the style of boiling in a steamer in $17th{\sim}18th$ century while in the 16th century,the an essay ofn fermenting flour in ‘Food Dimibang’ in 16th century had indicated it was cooked as the style ofby steaming in a rice steamer. Also, Mandu may have also contained the following: the thin-cut and boiled fish was cut out thin to put into the filling and boiled down, made by putting in added pine nuts after making bbeef jerky or boiled- down meat, fish, or shellfish itself to extractsand mold mandu only the ingredients combined withto put on starch powder, and then boiled down and put on pine nut powder finally, after it or cooled it wasdown to be eaten by dipping in vinegar soy sauce. In conclusion, many different types of mandu were made during the Joseon era using a variety ofwhile the ones using such various ingredients. are also one type of mandu.

• Journal of fish pathology
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• v.11 no.1
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• pp.51-60
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• 1998

Concerned to the lyfe cycle of Ichthyophthirius multifiliis, the experimental infection and development of the parasites were studied in the several freshwater cultured fishes. Opitimum conditions for the propagation of the parasite by serial passage with the rainbow trout fry was observed. Visiable white spots were examined in the body surface, fins and gills of the healthy fries, and a stable infection has been maintained for 2 months in the experimental system (Temperature: $18{\pm}1^{\circ}C$ DO: 7-7.5 ppm; pH: $7{\pm}0.2$). Induction conditions for artificial infection of the parasite by interms of the host fishes, stages of the parasites, and rearing temperature regimes was investigated. Rainbow trout fries showed a positive infection which was resulted from exposure of theront at $18^{\circ}C$. The rainbow trout fries induced white spots on the body surface at 3-7 days exposure to the theronts at $18^{\circ}C$. It was found that exposure of the rainbow trout fries exposed to 1,000 theronts per fish (10 theront/ml) for 45-60 minutes at $18^{\circ}C$ would consistently produce infection. Perfect infection (100%) was induced when the fries were exposed to 1,500 theront per fish (15 theront/ml) under laboratory condition. Development of I. multifiliis in the rainbow trout was observed for 7 days postexposure (PE). The parasite increased in average diameter from $54{\mu}m$ on the 1st day PE to $426{\mu}m$ on the 7th day PE. In the initial infestation period, the parasites were found on the gill epithelium, and on the 3rd day PE they invaded into the basal part of the gill filament adjacent to the major blood vessels, particularly the afferent vessels. Morphological change of buccal apparatus were observed on the 2nd day PE. Contractile vacuoles were more prominent on the 4th day PE, and they had notable changes on the 7th day PE.

• Parasites, Hosts and Diseases
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• v.55 no.3
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• pp.287-294
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• 2017

The present study was performed to record new definitive hosts of Isthmiophora hortensis, and to describe morphological characteristics derived from a variety of worm samples for clarification of its taxonomic validity. Morphological characteristics with dimensions were observed in worm samples (n=21) from naturally infected wild animals, including a raccoon dog Nyctereutes procyonoides from Gimhae-si (City), Gyeongsangnam-do, stray cats and a striped field mouse from several localities, and a wild boar Sus scrofa, from Gurye-gun (County), Jeollanam-do. In addition, adult flukes (n=45) recovered in albino rats experimentally infected with the metacercariae from a freshwater fish species were also subjected to morphological studies. The mean ratios of the body length (BL) to body width (BW) were 5.86 and 5.76 in worms from wild animals and experimental rats, respectively. Those of the ventral sucker to oral sucker were 2.92 and 3.01 in worms from 2 groups. The mean percentages of the hindbody length (HBL) to BL were 42.1 and 41.2 in 2 groups. Those of uterine fields to BL were 9.8 and 12.2 in the 2 worm groups. By the present study, the 2 species of wild animals, the raccoon dog and wild boar, have been added as new definitive hosts for I. hortensis. The morphological characteristics of adult flukes derived from a variety of host source were redescribed to support the taxonomic validity of this echinostome species.

• Fisheries and Aquatic Sciences
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• v.20 no.8
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• pp.16.1-16.8
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• 2017

The exportation of cultured olive flounder (Paralichthys olivaceus) in Korea has been recently decreasing due to the infections with a myxozoan parasite Kudoa septempunctata, and there is a strong demand for strict food safety management because the food poisoning associated with consumption of raw olive flounder harbouring K. septempunctata has been frequently reported in Japan. The life cycle and infection dynamics of K. septempunctata in aquatic environment are currently unknown, which hamper establishment of effective control methods. We investigated sea water and marine invertebrates collected from olive flounder farms for detecting K. septempunctata by DNA-based analysis, to elucidate infection dynamics of K. septempunctata in aquaculture farms. In addition, live marine polychaetes were collected and maintained in well plates to find any possible actinosporean state of K. septempunctata. The level of K. septempunctata DNA in rearing water fluctuated during the sampling period but the DNA was not detected in summer (June-July in farm A and August in farm B). K. septempunctata DNA was also detected in the polychaetes Naineris laevigata intestinal samples, showing decreased pattern of 40 to 0%. No actinosporean stage of K. septempunctata was observed in the polychaetes by microscopy. The absence of K. septempunctata DNA in rearing water of fish farm and the polychaetes N. laevigata intestinal samples during late spring and early summer indicate that the infection may not occur during this period. N. laevigata was suspected as the possible alternate invertebrate host of K. septempunctata, but the actinosporean stage was not found by well plate method and further studies will be necessary. This research provides important baseline information for understanding the infection dynamics of K. septempunctata in olive flounder farms and further establishment of control strategies.

• Parasites, Hosts and Diseases
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• v.26 no.3
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• pp.199-206
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• 1988

An epidemiological study was performed to know the infection status of 5. hortense among the human residents and fish intermediate hosts in Chongsong·gun, Kyongsangbuk·do. Out of 263 people examined, 59(22.4%) were positive for the eggs of E. hortense. Total 152 fresh water fishes (9 species) were ellamined, and Odentobutis obscura interrupts, Misgurnus an- guiIZicaudatus and Squalidus coreanus were found to harbour the metacercariae of E. hortense. Juvenile or adult E. hertense were expelled from 35 out of 54 cases treated with pragiquantel. The number of worms Per individual ranged from 1 to 649, with the average burden of 51. The eosinophil count of infected persons ranged 1∼24% (6% in average). The number of eggs per day Per worm (EPDPW) in human host was estimated about 1,478. The results showed that Chongsong-gun is an endemic area of E. hortense.

• Korean Journal of Veterinary Research
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• v.33 no.2
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• pp.277-283
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• 1993

In the present study, observations were made on the life-history of Lymnaea viridis under laboratory conditions, involving incubation period of the eggs and their hatching rate, shell length of the newly hatched snails, sexual maturity, size of the snails when the snail produced the first egg-mass, the number of eggs in each egg-mass, egg-laying, ovipostion, growth rate of the snails, and longevity of the snail. At temperatures between $19.8^{\circ}C$ to $22.5^{\circ}C$, incubation period of the eggs occupied 10~12 days, and after beginning of hatching, all young snails emerged completely from the egg-mass within 5 days. The hatching rate was 88%. The average shell length of the newly hatched snails was about 0.064cm. The rate of growth was extraordinarily rapid under good laboratory conditions. When two snails were reared in one culture vessel($20{\times}15{\times}5cm$) with blue-green algae at about $22^{\circ}C$, snail growth was optimal, taking 37 days to reach 1.2cm in shell length. Sexual maturity reached in about 19 days. The size of the snails at sexual maturity was $0.78{\pm}0.05cm$ in length and $0.47{\pm}0.04cm$ in width. The first egg-masses produced were $0.59{\pm}0.22cm$ in length and $0.34{\pm}0.08cm$ in width, and contained 7~38 eggs. The eggs are usually laid in water. The egg-laying was affected by food and temperature. Snails fed with blue-green algae at about $22^{\circ}C$ produced larger egg-masses than the snails fed with fish food at about $26^{\circ}C$. Under conditions of continuous activity and growth, the maximum expectation of life appears to be 109~350(mean 230) days. And the shell length of snails at death were 1.39~1.64cm.

• Korean Journal of Animal Reproduction
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• v.25 no.2
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• pp.171-180
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• 2001

Compared to other gene transfer system, the advantages of retrovirus-mediated gene transfer are technical ease, efficient expression and genetic stability. Despite the high potency of the retrovirus vector system in gene transfer, one of the drawbacks is a difficulty in concentration of virus stock. To overcome this problem, we tested a new retrovirus vector system producing the progeny retrovirus particles encapsidated with VSV-G (vesicular stomatitis virus G glycoprotein). The infectivity of this virus was not sacrificed by ultracentrifugal concentration and the host cell range extended from all mammalian to fish embryos. Virus titer after 1,000 x concentration was more than 10$^{8}$ CFU/ $m\ell$ on most of the target cell lines. We applied this pantropic viruses in transgenic chicken production by injecting the concentrated (100$\times$) stock into subgerminal cavity of stage X chicken embryos. The survival rate of chicken embryos after injection was about 20% and gene integration rate in surviving embryos was scored almost 100%. Analyses of RT-PCR and fluorescence microscopy, however, showed no evidence of the transgene expression.

• The Korean Journal of Ecology
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• v.21 no.1
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• pp.97-103
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• 1998

Yedang lake has so plentiful pondage and many species of fresh-walter-fish that a large number of people crowd around there all the year round. Most of them are not used to prevent tsutsugamushi disease spreading by chigger(T-mite). Accordingly, this study was carried out in order to obtain basic materials for prevention of this disease and the results are summarized as follows. The number of wild rat that captured in surveyed areas was sixty five(apodemus agrarius: 63, Rattus norvegicus: 2) during the period of a year and ration of captured rats to trap was about 8%. The dominant species of T-mite in all the surveyed areas were Leptotrombidium pallidum and L. palpale and the total number collected was 5782.9 with one family, two genra, eight species. The density of T-mites that collected from rats in three areas(Nodongri, Hatanbangri, Kyochonri) appeared $743.3{\pm}80.4,\;847.2{\pm}86.2\;and\;869.6{\pm}86.4,\;and\;in\;soil\;149.5{\pm}13.9,;154.7{\pm}14.7\;and\;182.4{\pm}20.8$ respectively. On the whole, the number of T-mite that collected from the rats was about three times as much as it in soil. The comparison of individual number of T-mite per a rat collected in three surveyed sites(A,B and C) showed 126.7, 243.1, 258.6 and per $2,000cm^3$ of soil 12.7, 12.7, 54.6, 103.5 respectively. In other word, the number of T-mite at site A and B is smaller than that at area C, thus comparing habitats of three sites each other, C is better living environment of rat and mite than that of A or B. Seasonl occuarance of t-mite that is from rat gradually was increased toward winter and showed the peak to January, and decreased since March but it in soil was inverse proportion to it from rats, because the larvae of T-mite that was hatched in soil was transmited to host in order to suck the body fluid.

• Journal of Environmental Science International
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• v.16 no.3
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• pp.277-285
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• 2007

The purpose of this study is to analyze seasonal distribution of insects, birds, fishes, and benthos for Gongji stream in Chuncheon and to explore some strategies towards creating a desirable close-to-nature stream. The number of species occurred in the study stream was 23 for butterflies and 149 for other insects, 23 for birds, 15 for fishes, and 47 for benthos. The study stream was dominated by Artogenia rapae, Polygonia caureum, Cyntia cardui for butterflies, and Passer montanus, Anas crecca, Anas platyrhynchos for birds. The dominant species were Rhynchocypris oxycephalus, Zacco plutypus, Carassius auratus for fishes, and Limnodrilus sp. 2, Chiranumus sp. 2, Hydropsyche KUb for benthos. Attracting insect species of higher density requires planting of host plant species and stabilization of plant coverage against disturbances. To enhance species diversify and density of birds, it is desirable to provide diverse habitats including preservation of downstream wetland and natural vegetation, creation of sand bars and islands with sand and gravel, and restriction or traffic passing and parking. Restoring fish and benthos habitats needs improvement of water quality, layout of gravels and stones on sand-dominant streambed, and use of close-to-nature revetment techniques.

• Animal Systematics, Evolution and Diversity
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• v.19 no.1
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• pp.57-93
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• 2003

Twelve species in five genera of parasitic copepods are recorded from seventeen species of freshwater fishes of Korea. They are Ergasilus coniformis n. sp., Ergasilus ventriosus n. sp., E. briani Markewitsch, E. peregrinus Heller, Neoergasilus joponicus (Harada) N. lonsispinosus Yin, N. inflatus Yin, N. bullatus n. sp., N. angustus n. sp., Sinergasilus undulatus (Markewitsch), Lernaea cyprinacea L., and Lamproglena chinensis $Y{\"{u}}.$ As the most prevalent parasitic copepod, Neoergasilus japonicus is found to parasitize as many as ten species of freshwater fishes in Korea. The fish Zacco platypus, from which seven species of parasitic copepods are discovered, turned out to be the most preferred host of the parasitic copepods in this country. Full descriptions are given of the new species and new records of Korea. Lamproglena chinensis, an incompletely known species, is also redescribed.ibed.