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Optimization of One-Step Dilution Method of Vitrified Bovine IVM/IVF/IVC Blastocysts (초자화 동결된 체외생산 소 배반포기배의 1 단계 융해 방법의 적정화)

  • Lee, K.S.;Kim, E.Y.;Nam, H.K.;Park, S.Y.;Park, E.M.;Yoon, S.H.;Park, S.P.;Chung, K.S.;Lim, J.H.
    • Korean Journal of Animal Reproduction
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    • v.24 no.1
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    • pp.89-95
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    • 2000
  • This study was to establish an effective dilution technique in a vitrification of bovine blastocysts for the field trial. For vitrification, blastocysts were exposed in glycerol (G) and ethylene glycol (EG) mixture in m-DPBS supplemented with 10% FBS. Blastocysts were first exposed to 10% (v/v) G for 5 min, and subsequently were transferred to 10% G plus 20% EG (v/v) for 5 min. Finally, embryos were transferred to 25% G plus 25% EG (v/v) for 30 see and were placed in nitrogen vapor for 3 min, and then were plunged into L$N_2$. At thawing, the straw containing blastocysts was placed in air for 10 sec, and then plunged into a water bath at $25^{\circ}C$ until all ice had disappeared. They were placed in $25^{\circ}C$ and 36$^{\circ}C$ water according to treatment group for different time. Also, in vitro survival was assessed by the re-expansion and hatched rates at 24 hand 48 h postwarming, respectively. The results obtained in these experiments were summarized as follows; 1) In the survival rates of vitrified bovine blastocysts according to different dilution time at thawing, the data of 1 min group (86.6, 56.6%) were higher than those of other treatment groups (2 min; 93.5, 35.4%, 2.5 min; 76.9, 30.7%, 3 min; 88.8, 36.1% and 3.5 min; 83.7, 8.1%). 2) When the in vitro survival of vitrified groups according to different developmental stage was examined at 48 h after thawing using 1 min dilution method, the hatching rates of fast developed embryos (expanded blastocyst: 81.3%: early hatching blastocyst: 86.2%) were higher than that of delayed developed one (early blastocyst: 46.6%). 3) In addition, when the in vitro survival of vitrified groups according to different embryo age was compared, the hatched rates at 48 h after thawing of Day 7 (66.6%) and Day 8 embryos (60.0%) were significantly higher than that of Day 9 embryos (22.7%) (P<0.05). These results demonstrate that vitrified bovine IVM/IVF/IVC blastocysts can be successfully survived in vitro using one-step dilution (1 min) method.

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Park Yeol·Kaneko Humiko Case and Performance (박열·가네코 후미코 사건과 퍼포먼스)

  • Baek, Hyun-Mi
    • Journal of Popular Narrative
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    • v.25 no.2
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    • pp.117-167
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    • 2019
  • The aim of this article is to illuminate the Park Yeol(朴烈)·Kaneko Humiko(金子文子) Case from the perspective of performance, by analyzing newspapers published in Colonial Korea. The Park Yeol·Kaneko Humiko Case include the High Treason Incident(大逆事件) case and the mysterious photo(怪寫眞) case that occurred in Tokyo in Imperial Japan from 1923 to 1926. Even though Park Yeol·Kaneko Humiko were individually imprisoned during this period, they proceeded to act shrewdly and preposterously as performers. First, they made the trial itself into an astonishing case by donning traditional Korean clothes and insisting on using the Korean language in Japanese Imperial Court. Second, they caused the judge in charge to accidentally take the so-called 'mysterious photo,' which later led to the collapse of the Japanese cabinet. The newspapers published in Colonial Korea served as unique stage on which Park Yeol and Kaneko Humiko performed. The newspaper articles reported on the public trials as if it were a drama, describing their clothes, look, and dialogue in public court. The news about them was published not as it occurred but in a plotted sequence because of a press ban, consequentially building suspense among readers. Meanwhile, the Korean newspaper editorials pointed out the injustice of the High Treason Incident, breaking down the Japanese judge's opinion. The Park Yeol·Kaneko Humiko Case was a social drama that revealed the disharmony that led to the breakdown of Taisho Democracy and imprinting national resistance in Japan as well as in Korea.

A Study on Image Copyright Archive Model for Museums (미술관 이미지저작권 아카이브 모델 연구)

  • Nam, Hyun Woo;Jeong, Seong In
    • Korea Science and Art Forum
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    • v.23
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    • pp.111-122
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    • 2016
  • The purpose of this multi-disciplinary convergent study is to establish Image Copyright Archive Model for Museums to protect image copyright and vitalize the use of images out of necessity of research and development on copyright services over the life cycle of art contents created by the museums and out of the necessity to vitalize distribution market of image copyright contents in creative industry and to formulate management system of copyright services. This study made various suggestions for enhancement of transparency and efficiency of art contents ecosystem through vitalization of use and recycling of image copyright materials by proposing standard system for calculation, distribution, settlement and monitoring of copyright royalty of 1,000 domestic museums, galleries and exhibit halls. First, this study proposed contents and structure design of image copyright archive model and, by proposing art contents distribution service platform for prototype simulation, execution simulation and model operation simulation, established art contents copyright royalty process model. As billing system and technological development for image contents are still in incipient stage, this study used the existing contents billing framework as basic model for the development of billing technology for distribution of museum collections and artworks and automatic division and calculation engine for copyright royalty. Ultimately, study suggested image copyright archive model which can be used by artists, curators and distributors. In business strategy, study suggested niche market penetration of museum image copyright archive model. In sales expansion strategy, study established a business model in which effective process of image transaction can be conducted in the form of B2B, B2G, B2C and C2B through flexible connection of museum archive system and controllable management of image copyright materials can be possible. This study is expected to minimize disputes between copyright holder of artwork images and their owners and enhance manageability of copyrighted artworks through prevention of such disputes and provision of information on distribution and utilization of art contents (of collections and new creations) owned by the museums. In addition, by providing a guideline for archives of collections of museums and new creations, this study is expected to increase registration of image copyright and to make various convergent businesses possible such as billing, division and settlement of copyright royalty for image copyright distribution service.

An Analytical Study on the Growth of Anoplocnemis dallasi Kiritschenko (장수허리노린재(Anoplocnemis dallasi Kiritschenko)의 成長分析)

  • Park, Sang Ock;Lee, Chang Eon
    • The Korean Journal of Zoology
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    • v.14 no.3
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    • pp.139-158
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    • 1971
  • This study was initiated to observe the growth of the lengths of the body, the antenna, the rostrum, the fore leg, the middle leg, the hind leg and the width of pronotum in the postembryonic development of Anoplocnemis dallasi. The specimens measured were fifty in the first instar larva and seventy for each instar from the second instar larva to adult stage. The authors applied the growth formula and the relative growth formula to analyse the changing pattern of the growth of each part. In this paper, having applied the formulae the y=a+bt+$ct^2$ for the absolute growth and $y=bx^{\alpha}$ for the relative growth, we obtained the following results: $\circled1$ The growth quantity: The rostrum shows the slowest, straight increase, but the other shows the curving increase. The body, the hind leg, the middle leg, the fore leg, the antenna, the width of pronotum and the rostrum are systematically slow. $\circled2$ The ratio of the growth quantity: The increase rate of the rostrum shows the straight, while the other shows the curving patterns. The largest value is the increase rate of the width of pronotum (8.816 times) and the smallest one is the rostrum (3.054 times). $\circled3$ The growth ratio for each instar: The maximal ratio of the growth quantity is in the young instar larva, but the minimal one is in an advanced instar larva. $\circled4$ The growth rate: The antenna shows a decrease pattern, while the other an increase pattern. $\circled5$ The specific growth rate: The rates of the antenna, the for leg, the rostrum, the hind leg and the middle leg show a decrease pattern. In the width of pronotum and the body, they increase in the terms of the young instar larva and they increase later. The antenna shows the most rapid decrease, and the fore leg, the rostrum, the hind and the middle leg are slow in order. $\circled6$ The "$\alpha$" of the width of pronotum shows the strongest positive allometry, but the rostrum shows the weakest negative allometry to all parts. $\circled7$ The coefficient of the relative growth of each part shows a parallel fashion in the relative growth to body length, to width of pronotum, to antenna, to rostrum, to fore leg, to middle leg and to hind leg. $\circled8$ If the coefficient of the relative growth, the growth ratio ($\alpha$) increase, the initial growth index (b) is disposed to decrease and vice versa. $\circled9$ The growth center is in the terms of the young instar larva in case of the negative allometry, but it is in the terms of an advanced instar larva in case of the positive allometry.

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Quality Characteristics of Prepared Rehmannia Root with Four Domestic Cultivars (국내 육성 품종별 숙지황의 품질 특성)

  • Kim, Yae Jin;Han, Sin Hee;Ma, Kyungho;Hong, Chung-Oui;Han, Jong-Won;Lee, Sang Hoon;Chang, Jae Ki;Lee, Jun soo;Jeong, Heon-Sang
    • Korean Journal of Breeding Science
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    • v.51 no.4
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    • pp.386-394
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    • 2019
  • Rehmannia glutinosa, one of the major medicinal crops in Korea, can be classified into three types: fresh, dried and prepared Rehmannia root. In this study, the quality characteristics of prepared rehmannia root were evaluated using four different cultivars that are commonly used in the market. In making prepared rehmannia root, roots of Jihwang 1, Kokang, Togang, and Dagang were dried, soaked in rice wine, and steamed nine times. At each stage, physiochemical properties were analyzed, including yield, which is one of the most important industrial factors to consider. The yield was the highest in Togang at 23.61% and the lowest in Dagang at 21.16%. These yield values showed a highly negative correlation with the moisture content of roots. The fructose and glucose contents were increased during the 3rd, 4th and 5th steaming but then decreased. The sucrose, raffinose, and stachyose content gradually decreased during the first three steaming and were not detected during the 4th steaming. Additionally, the catalpol content was not detected after the 4th steaming. On the contrary, the 5-hydroxymethylfurfural content was not detected in the raw root but increased during the steaming. Jihwang1 and Togang exceeded the 0.1% Korean Pharmacopoeia standard after the 5th steaming, reaching it faster than did the other cultivars. Overall, Togang was the optimal cultivar considering the overall characteristics of its high yield and short steaming time. These results could provide useful information for the industrial use of prepared Rehmannia root based on the requirements and characteristics of each cultivar.

Predictions of VO2max Using Metabolical Responses in Submaximal Exercise and 1,200 m Running for Male, and the Validity of These Prediction Models (성인 남성의 최대하 운동시 대사반응 및 1,200 m 달리기 기록을 이용한 최대산소섭취량 추정식 개발 및 타당도)

  • Im, J.H.;Jeon, Y.J.;Jang, H.K.;Kim, H.J.;Kim, K.H.;Lee, B.K.
    • Exercise Science
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    • v.21 no.2
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    • pp.231-242
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    • 2012
  • The purpose of this study was to develop the prediction model of VO2max using submaximal metabolic responses from the Bruce protocol, HR responses at several stages and 1,200 m running record, and to compare and analyse the validity of these prediction models. The subjects were consisted of 255 male(133 male for 1,200 m running). They were participated maximal exercise testing with Bruce protocol, and the metabolic responses were measured in the end of the first(3 minute), second stage(6 minute), and 1,200 m running record. Measurement items were VO2(㎖/kg/min), VCO2(㎖/kg/min), VE(L/min), HR(bpm) of 3 and 6 minute, time to HR 150 bpm and 170 bpm, HR difference between Bruce protocol 6 and 3 minute, 1,200 m running record. Analyzing with all variables using enter method, the multiple R of total variable model was 0.642(p<.01), SEE was 4.38 ㎖/kg/min, CV was 10.8 %, but multicolinearity was appeared. The multiple R of 3 minutes model 1 and model 2 were 0.341 and 0.461, SEE was 6.05 and 5.72 ㎖/kg/min, CV was 14.9 and 14.1%, and multicolinearity did not appeared. The multiple R of 6 minutes model 1 and model 2 were 0.350 and 0.456, SEE was 6.03 and 5.74 ㎖/kg/min, CV was 14.9 and 14.2%, and multicolinearity did not appeared. The R of HR 170 and HR 170 model were 0.151 and 0.154, SEE were 6.36~6.37 ㎖/kg/min, CV were 15.7%. The R of 1,200 m running model was 0.444, SEE was 4.82 ㎖/kg/min, CV were 11.9%. In conclusion, with considering usefulness and convenience through the validity of these prediction models, the prediction model of VO2max recommended 6 and 3 minute model, and the validity of HR model and 1,200 m running model were moderately low.

The Composition and Performance of Suryukjae (수륙재의 구성과 연행 -진관사 수륙재를 중심으로)

  • Hong, Teahan
    • (The) Research of the performance art and culture
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    • no.23
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    • pp.347-368
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    • 2011
  • This article is a review of the composition pattern of Suryukjae, which is one of the Buddhist ceremonies, and a consideration of the performance process. As one of the ceremonies leading the dead to heaven performed in the Buddhist circle, Suryukjae had been performed in Buddhist temples nationwide, but currently, it remains only in several Buddhist temples. Suryukjae is composed as follows. First, the early part of Suryukjae is a stage of preparing Suryukjae and ensuring legitimacy; thus, it has no detailed Jaecha except for Gwanyok. It is made up of Onghoge and Dage, has no Somun, and is centered on Yojabbara. The middle part of Suryukjae is a part in which Suryukjae is performed on a full-scale, which wishes the achievement of the goal of Suryukjae. It is made up of Geobul, Dage, and Somun, and is centered on Sadaranibara. Furthermore, this part delineates the flow of the Jaecha concerned as it contains detailed Jaecha. Meanwhile, in the middle part of Suryukjae, there is only detailed Jaecha called Sajadanman Bongsong (sending off), and the rest parts including Orodan, upper part, middle part, and lower part, in which there is no detailed Jaecha called Bongsong. The fact that there is no Bongsong in this part means all Bongsong is made in Heuihyang Bongsong, which is the last Jaecha. This implies that Saja, which is enshrined in Sajadan, is the essence of the achievement of the goal of Suryukjae. Only when there is Saja, Muju, Yuju, and Gohon (the meaning of all spirits) can be led to heaven. Also, from a rough perspective, this part is connected to other Chundojae (ceremony for sending off the dead to heaven) in Korea. There is a geori(Jaecha) that calls in Saja also in Jinogigut (exorcism) of Seoul. Then, although various gods from the otherworld are coming in in succession, to which Saja enters clearly shows a process in that Saja returns to the next world with the dead through . Just as demonstrates a process in that Saja returns to the next world with the dead in Jinogigut, also in Suryukjae, Saja needs to return to the next world with Gohon; therefore, at least Sajadan has detailed Jaecha called Bongsong. That is, this means that although Suryukjae of Buddhism is different from other Chundojae in terms of the composition of Jaecha, its notion about Saja is the same.

Evaluation of K-Cabbage Model for Yield Prediction of Chinese Cabbage in Highland Areas (고랭지 배추 생산 예측을 위한 K-배추 모델 평가)

  • Seong Eun Lee;Hyun Hee Han;Kyung Hwan Moon;Dae Hyun Kim;Byung-Hyuk Kim;Sang Gyu Lee;Hee Ju Lee;Suhyun Ryu;Hyerim Lee;Joon Yong Shim;Yong Soon Shin;Mun Il Ahn;Hee Ae Lee
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.25 no.4
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    • pp.398-403
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    • 2023
  • Process-based K-cabbage model is based on physiological processes such as photosynthesis and phenology, making it possible to predict crop growth under different climate conditions that have never been experienced before. Current first-stage process-based models can be used to assess climate impact through yield prediction based on climate change scenarios, but no comparison has been performed between big data obtained from the main production area and model prediction so far. The aim of this study was to find out the direction of model improvement when using the current model for yield prediction. For this purpose, model performance evaluation was conducted based on data collected from farmers growing 'Chungwang' cabbage in Taebaek and Samcheok, the main producing areas of Chinese cabbage in highland region. The farms surveyed in this study had different cultivation methods in terms of planting date and soil water and nutrient management. The results showed that the potential biomass estimated using the K-cabbage model exceeded the observed values in all cases. Although predictions and observations at the time of harvest did not show a complete positive correlation due to limitations caused by the use of fresh weight in the model evaluation process (R2=0.74, RMSE=866.4), when fitting the model based on the values 2 weeks before harvest, the growth suitability index was different for each farm. These results are suggested to be due to differences in soil properties and management practices between farms. Therefore, to predict attainable yields taking into account differences in soil and management practices between farms, it is necessary to integrate dynamic soil nutrient and moisture modules into crop models, rather than using arbitrary growth suitability indices in current K-cabbage model.

Development of Cloud Detection Method Considering Radiometric Characteristics of Satellite Imagery (위성영상의 방사적 특성을 고려한 구름 탐지 방법 개발)

  • Won-Woo Seo;Hongki Kang;Wansang Yoon;Pyung-Chae Lim;Sooahm Rhee;Taejung Kim
    • Korean Journal of Remote Sensing
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    • v.39 no.6_1
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    • pp.1211-1224
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    • 2023
  • Clouds cause many difficult problems in observing land surface phenomena using optical satellites, such as national land observation, disaster response, and change detection. In addition, the presence of clouds affects not only the image processing stage but also the final data quality, so it is necessary to identify and remove them. Therefore, in this study, we developed a new cloud detection technique that automatically performs a series of processes to search and extract the pixels closest to the spectral pattern of clouds in satellite images, select the optimal threshold, and produce a cloud mask based on the threshold. The cloud detection technique largely consists of three steps. In the first step, the process of converting the Digital Number (DN) unit image into top-of-atmosphere reflectance units was performed. In the second step, preprocessing such as Hue-Value-Saturation (HSV) transformation, triangle thresholding, and maximum likelihood classification was applied using the top of the atmosphere reflectance image, and the threshold for generating the initial cloud mask was determined for each image. In the third post-processing step, the noise included in the initial cloud mask created was removed and the cloud boundaries and interior were improved. As experimental data for cloud detection, CAS500-1 L2G images acquired in the Korean Peninsula from April to November, which show the diversity of spatial and seasonal distribution of clouds, were used. To verify the performance of the proposed method, the results generated by a simple thresholding method were compared. As a result of the experiment, compared to the existing method, the proposed method was able to detect clouds more accurately by considering the radiometric characteristics of each image through the preprocessing process. In addition, the results showed that the influence of bright objects (panel roofs, concrete roads, sand, etc.) other than cloud objects was minimized. The proposed method showed more than 30% improved results(F1-score) compared to the existing method but showed limitations in certain images containing snow.

The Impact of Corporate Culture on Job Stress : A Mediating Variable of Overtime and Organizational Trust (기업문화가 직무스트레스에 미치는 영향 : 주당 초과 근로시간과 조직신뢰의 매개변수)

  • Jeon, Young-jun
    • Journal of Venture Innovation
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    • v.6 no.3
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    • pp.149-164
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    • 2023
  • Today, when innovation and creativity become increasingly important, management of human resources is a key factor for corporate performance and competitive advantage. Corporate are implementing and introducing various types of support methods for members to achieve goals and improve organizational performance. Organizational culture and organizational trust affect the cognitive and emotional state of members. Furthermore, it can bring about changes in organizational performance such as job stress and job satisfaction. From an institutional point of view, work-life balance is also a major factor affecting organizational performance. The imbalance between work and life leads to a decline in organizational performance, such as decreased morale and dissatisfaction with work. In relation to work-life balance, the low birth rate problem intensified and the importance began to emerge. Therefore, the government has implemented various policy support for workers' work-life balance, and the "52-hour workweek" is a representative example. This study analyzed the effect of organizational culture applying the competitive value model on workers' job stress. In addition, the mediating effects of overtime work per week and organizational trust were analyzed. Job stress corresponds to a prerequisite stage that affects job commitment, job satisfaction, and turnover intention. However, research measuring job stress by organizational performance is insufficient. In addition, there are few studies analyzing the relationship between overtime and organizational performance. Considering this, it is necessary to understand the influence relationship. The results of the study are as follows. First, a hierarchical culture increases the job stress of workers. On the other hand, innovation-oriented, relationship-oriented, and competition-oriented corporate culture reduce job stress. Second, a hierarchical culture has reduced trust in the organization, and other organizational cultures have increased trust in the organization. Third, relationship-oriented and competition-oriented organizational culture reduced overtime. Innovation-oriented, hierarchical-oriented culture increased overtime Fourth, organizational trust and overtime have the effect of mediating organizational culture and job stress. Based on these analysis results, this study presented academic and political implications.