• Journal of Korean Society of Environmental Engineers
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• v.35 no.7
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• pp.518-524
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• 2013

A pilot-scale test-bed was operated employing three soils with different grain sizes dredged from the Nakdong River to obtain the design and operation parameters of the multi-purpose filtration pond, such as the filtrate productivity of the filter sand, the appropriate removal period of the surface clogging and the contaminant removal efficiency. The cross-flow velocities were applied stepwise ranging from 0 to 40 cm/sec in order to simulate the various velocities in the artificial stream of the pond. Results showed that a filtrate production rate of 5~3 $m^3/m^2-day$ was maintained by removing the surface clogging every 7 to 13 days and that the filtrate quality was not affected by the factors of the filtrate production rate, the grain size of the filter sand and the cross-flow velocity. Results also showed that most of the removal occurred within 50 cm of the top soil and that the removal efficiencies with the filtration distance of 2.4 m were 80~95% for turbidity, 20~30% for COD, 75~90% for BOD, 5~20% for total nitrogen and 20~60% for total phosphorus, which suggested that particulate matters had a high removal efficiency.

• Journal of Korean Society of Environmental Engineers
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• v.27 no.3
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• pp.302-308
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• 2005

Nitrate is one of common contaminants frequently found in the bank filtrate. Biological autotrophic denitrification into permeable reactive barrier(PRB) system to reduce nitrate concentration in bank filtrate was implanted. The objectives of research are to investigate effect of inoculation, to evaluate alternative alkalinity sources, and to determine effect of hydraulic characteristics, such as retention time, flow rate on the performance of semi-pilot PRB system. Semi-pilot scale biological PRB system was installed using elemental sulfur and limestone/oyster shell as reactive materials near Nakdong River in Kyoungnam province, Korea. Nitrate concentration in bank filtrate was reduced by indigenous microorganisms in oyster shell as welt as by inoculating microorganisms isolated from the sludge of an anaerobic digester in a wastewater treatment plant. Oyster shell as well as limestone can be used as an alkalinity source. However, oyster shell resulted in suspended solids of effluent. As the flow rate in the system increased from 66 to 132 mL/min and accordingly the residence time decreased from 15 to 7.5 hours, nitrate concentration in effluent increased and nitrate removal efficiencies decreased from 75 to 58% at the fixed thickness of 80 cm of PRB.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.219-229
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• 2018

We isolated Streptomyces scopuliridis KR-001 that produced herbicidal active metabolite(s) against several grass and broad leaf weeds. In this study, potential as natural herbicide of mutant N-29 broth filtrate of S. scopuliridis KR-001 was investigated to Ambrosia artemisiifilia in a greenhouse and field condition. The broth filtrate of mutant N-29 by foliar application showed a strong herbicidal activity to A. artemisiifilia with leaf stage in a greenhouse condition. Also, field trial of foliar application within treatment range had effectively controlled with early and middle stage of A. artemisiifilia at the natural habitats. Phytotoxic symptoms of mutant N-29 broth filtrate by foliar application were wilting and discoloration, and burn-down of leaves and finally plant death. These results suggest that mutant N-29 broth filtrate is considered possible as a natural herbicide for controlling environmentally friend to invasive alien plant such as A. artemisiifilia and may provide a new lead molecule for a more efficient herbicide.

• Journal of Microbiology
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• v.42 no.1
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• pp.37-41
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• 2004

The textile industry wastewater has been decolorized efficiently by the white rot fungus, Irpex lacteus, without adding any chemicals. The degree of the decolorization of the dye effluent by shaking or stationary cultures is 59 and 93%, respectively, on the 8th day. The higher level of manganese-dependent peroxidase (MnP) and non-specific peroxidase (NsP) was detected in stationary cultures than in the cultures shaken. Laccase activities were equivalent in both cultures and its level was not affected significantly by the culture duration. Neither lignin peroxidase (LiP) nor Remazol Brilliant Blue R oxidase (RBBR ox) was detected in both cultures. The absorbance of the dye effluent was significantly decreased by the stationary culture filtrate of 7 days in the absence of Mn (II) and veratryl alcohol. In the stationary culture filtrate, three or more additional peroxidase bands were detected by the zymogram analysis.

• BMB Reports
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• v.36 no.2
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• pp.185-189
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• 2003

Chitinase (EC 3.2.1.14) was isolated from the culture filtrate of Streptomyces sp. M-20 and purified by ammonium sulfate precipitation, DEAE-cellulose ion-exchange chromatography, and Sephadex G-100 gel filtration. No exochitinase activity was found in the culture filtrate. The molecular mass of the purified chitinase was 20 kDa, estimated by a sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and was confirmed by activity staining with Calcofluor White M2R. Chitinase was optimally active at pH of 5.0 and at $30^{\circ}C$. The enzyme was stable from pH 4 to 8, and up to $40^{\circ}C$. Among the metals and inhibitors that were tested, the $Hg^+$, $Hg^{2+}$, and p-chloromercuribenzoic acid completely inhibited the enzyme activity. The chitinase activity was high on colloidal chitin, chitotriose, and chitooligosaccharide. The purified chitinase showed antifungal activity against Botrytis cinerea, and lysozyme activity against the cell wall of Botrytis cinerea.

• Microbiology and Biotechnology Letters
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• v.24 no.5
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• pp.540-545
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• 1996

A facultative anaerobe Enterococcus faecium 19-46-4 was used to study the production of an antimicrobial substance in anaerobic conditions. Major part of the antibiotic activity was found in the culture filtrate of the bacterium. The active compound was extracted by an equal volume of iso-butanol and concentrated in vacuo (at 50$\circ$C) before purification by C-18 liguid column chromatography and HPLC. A chromatographically pure compound was obtained by two passages of HPLC columns, The compound appeared as a pale-yellow powder. The yield was about 2.5 mg 1$^{-1}$ culture filtrate. The compound was named as KIST 194. KIST 194 were identified as cholic acid (3$\alpha$, 7$\alpha$, 12$\alpha$-trihydroxy-5$\beta$-cholan 24-oic acid) based on its physico-chemical properties determined by UV, IR, $^{1}H-NMR, $^{13}$C-NMR, El-MS and LC-MS.

• Microbiology and Biotechnology Letters
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• v.32 no.4
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• pp.371-374
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• 2004

A bacterium producing the $\beta$-mannosidase was isolated from intestine of fresh fish. The isolate YB-29 has been identified as Chryseomeningosepticum on the basis on its 16S rRNA sequence, morphology and biochemical proper The $\beta$-mannosdiase activity was detected in both the culture filtrate and the cell extract of C. meningosepticum YB-29. The $\beta$-mannosidase of culture filtrate showed the maximum activity for hydrolysis of para-nitrophenyl-$\beta$-D-mannopyranoside at pH 5.0-6.0 and 55-$60^{\circ}C$. The enzyme was able to hydrolyze the oligomeric substrates such as mannobiose and mannotriose with higher activity for mannotriose than mannobiose.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.68-71
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• 2006

Seasonal fluctuation of groundwater level by pumping amount and stream discharge at the riverbank filtrate site adjacent to the Nakdong River in Daesan-Myeon was characterized. Groundwater level fluctuation shows increase in wet season (June, July, August and September) and decrease in dry season (the other months). Seasonal variation of pumping amount shows similar trend to the groundwater fluctuation due to higher consumption of potable water in summer. The relation of specific capacity, Nakdong River and pumping quantity was analyzed. The logarithmic relationship between specific capacity and the stream discharge gives high correlation coefficient, 0.96. This fact indicates that the increase of stream discharge rate reduces the rate of drawdown in the pumping area in wet season.

• Korean Journal Plant Pathology
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• v.13 no.2
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• pp.132-137
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• 1997

Phytotoxicity of the culture filtrate and culture conditions for the production of the phytotoxin by Exserohilum monoceras 92-044 were examined. The necrotic lesions were developed on the leaves of Echinochloa crus-galli within 48 hrs after inoculation of the culture filtrate, and the leaves were completely blighted within 5∼7 days. Maximum toxicity was found in the culture broth containing 20% V-8 juice. Phytotoxin accumulation and fungal growth reached their highest peak at around 11 days. Typical symptom appeared on the leaf of E. crus-galli within 48 hrs. Only a weak chlorosis appeared on rice, Arundinella hirta (THUNB) and henry crabgrass (Digitaria sanguinalis SCOP.), but no further symptom developed.

• Mycobiology
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• v.30 no.2
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• pp.96-101
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• 2002

Four pathogenic fungal isolates belonging to different genera including Alternaria, Fusarium and Curvularia were isolated from selected diseased weeds growing in the fields in Dakahalia district. The inoculum of these pathogenic fungi specific to weeds were cultured, standardized and formulated as alginate pellets containing mycelium plus culture filtrate. These mycoherbicides were evaluated for disease severity(DS). Maximum DS was obtained with the alginate pellets of mycelium filtrate Fusarium solani. Physiological changes of the treated weed were determined 5 aiid 10 days after treatments. As compared to the healthy weeds, all mycoherbicide formulations significantly decreased the amount of photosynthetic pigments and subsequently soluble and insoluble sugars in the infected weeds. The mycoherbicide formulation of F. solani had the greatest effect on lowering to the abovementioned amount in the leaves of Chenopodium murale. Generally, treatment of weed leaves with the specific mycoherbicide led to a highly significant increase in total phenol content when compared to the healthy control weed. C. murale infected with the mycoherbicide formulation of F. solani had higher levels of phenolic compounds than those other treated weeds particularly after 10 days of inoculation.