• Title/Summary/Keyword: field tolerance

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CGMMV Tolerance Test of CGMMV-CP Trangenic Watermelon Rootstock and Establishment of Transgenic Line (CGMMV-CP 형질전환 수박대목의 CGMMV 내성시험 및 계통확보)

  • Park, Sang-Mi;Kwon, Jung-Hee;Lim, Mi-Young;Shin, Yoon-Sup;Her, Nam-Han;Lee, Jang-Ha;Ryu, Ki-Hyun;Harn, Chee-Hark
    • Journal of Plant Biotechnology
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    • v.34 no.1
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    • pp.11-17
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    • 2007
  • Previously developed transgenic watermelon rootstocks (gongdae) inserted by CGMMV-CP were examined to test the virus tolerance levels. In the restricted plastic house, the $T_{3}$ watermelon rootstock showed tolerance to CGMMV until 70 days after inoculation on the leaves while the non-transformed watermelon rootstock became susceptible at 20 days after inoculation. In the field, tolerance efficiency of transgenic rootstocks maintained up to 40% at 71 days after contamination with CGMMV in the soil while all of the non-transformed rootstocks became susceptible at 37 days with the same condition. In the same field, transgenic rootstocks showed more tolerance to CGMMV than the non-transformed rootstocks as those were inoculated on the leaves, but it showed only 10 days delay before being susceptible. Therefore, transgenic rootstocks have a characteristic of delay effect against CGMMV susceptibility, rather than resistance character. From $T_{3}$ rootstocks homozygous for the CGMMV-CP horticulturally favorable individuals were selected for further breeding and a transgenic line was finally obtained at the $BC_{1}T_{5}$. A material transfer experiment was conducted to find out if the DNA, RNA or expressed protein in the transgenic rootstocks could move to the grafted scion (non-transformed watermelon, Super-Kumcheon). PCR, northern, and western blot analysis were performed and no evidence of transferring of those materials from rootstock to scion was ever found.

Electric Field Uniformity in Reverberation Chamber with 5 GHz Diffuser by Transmission Antenna (송신 안테나에 의한 5 GHz 이차 잔류 디퓨저를 적용한 전자파 잔향실의 내부 필드 균일도 변화)

  • Rhee, Eugene
    • Journal of the Semiconductor & Display Technology
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    • v.20 no.3
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    • pp.83-86
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    • 2021
  • In this paper, electric fields in electromagnetic reverberation chambers, which are used as a substitute facility for EM-free anechoic chambers, are analyzed. This paper focused on the 4-5 GHz band, which is expected to adversely affect equipment. To analyze the field uniformity inside the electromagnetic reverberation chamber, electric field strengths are sampled and finite-difference time-domain method was used for numerical analysis. Moreover, Quadratic residue diffuser was used to improve the characteristics of the electromagnetic reverberation chamber and the uniformity of the internal field strength. Standard deviation, tolerance characteristics, and partiality characteristics were compared while varying the aiming point of transmission antenna.

Two-year field monitoring shows little evidence that transgenic potato containing ABF3 significantly alters its rhizosphere microbial community structure

  • Nam, Ki Jung;Kim, Hyo-Jeong;Nam, Kyong-Hee;Pack, In Soon;Kim, Soo Young;Kim, Chang-Gi
    • Journal of Ecology and Environment
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    • v.41 no.3
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    • pp.99-106
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    • 2017
  • Background: Plants over-expressing Arabidopsis ABF3 (abscisic acid-responsive element-binding factor 3) have enhanced tolerance to various environmental stresses, especially drought. Using terminal restriction fragment length polymorphism (T-RFLP) analysis, we compared the rhizosphere-associated structures of microbial communities for transgenic potato containing this gene and conventional "Jopoong" plants. Results: During a 2-year field experiment, fungal richness, evenness, and diversity varied by year, increasing in 2010 when a moderate water deficit occurred. By contrast, the bacterial richness decreased in 2010 while evenness and diversity were similar in both years. No significant difference was observed in any indices for either sampling time or plant line. Although the composition of the microbial communities (defined as T-RF profiles) changed according to year and sampling time, differences were not significant between the transgenic and control plants. Conclusions: The results in this study suggest that the insertion of ABF3 into potato has no detectable (by current T-RFLP technique) effects on rhizosphere communities, and that any possible influences, if any, can be masked by seasonal or yearly variations.

Reliability estimation about quality assurance method of radiotherapy planning (방사선치료계획 정도관리 방법에 따른 신뢰도 평가)

  • Kim, Jeong-Ho;Kim, Gha-Jung;Yoo, Se-Jong;Kim, Ki-Jin
    • Journal of the Korea Safety Management & Science
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    • v.17 no.1
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    • pp.119-124
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    • 2015
  • According as radiation therapy technique develops, standardization of radiation therapy has been complicated by the plan QA(Quality Assurance). However, plan QA tools are two type, OADT (opposite accumulation dose tool) and 3DADT (3 dimensional accumulation dose tool). OADT is not applied to evaluation of beam path. Therefore tolerance error of beam path will establish measurement value at OADT. Plan is six beam path, five irradiation field at each beam path. And beam path error is 0 degree, 0.2 degree, 0.4 degree, 0.6 degree, 0.6 degree, 0.8 degree. Plan QA accomplishes at OADT, 3DADT. The more path error increases, the more plan QA error increases. Tolerance error of OADT path is 0.357 using tolerance error of conventional plan QA. Henceforth plan QA using OADT will include beam path error. In addition, It will increase reliability through precise and various plan technique.

Influence of Internode Strength of Rice Plant on the Lodging Tolerance (벼절간의 강도가 도복저항성에 미치는 영향)

  • 김연진;최수일;소재돈
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.1
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    • pp.94-99
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    • 1983
  • This experiment was conducted to study a cause of lodging on culm internode. The rice varieties were undergone on injuries by typo on 'Agnes' on Jeonbug Provincial in 1981 year. Long-culmed varieties had extreme lodging injury but short-culmed varieties were not serious. The lodging had weaken tolerance when culm internode was long, weight of internode per unit length was light, and culm diameter and culm wall were thin. The breaking weight of internode was light in lodging rice field. The internode length and the weight of internode per unit length between the breaking weight of internode had significant correlation. Lodging had high tolerance when the total nitrogen was low, phosphate, silica, cellulose and starch contents were high in internode. The difference of culm length and the weight of internode per unit length was high relationship with lodging.h lodging.

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Design of Reliable Adaptive Filter with Fault Tolerance Using TMS320C32 (TMS320C32를 이용한 고장허용을 갖는 신뢰 적응 필터 설계)

  • Ryoo, Dong-Wan;Seo, Bo-Hyeok
    • Proceedings of the KIEE Conference
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    • 2000.07d
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    • pp.2429-2432
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    • 2000
  • Adaptive filter algorithm has been used for plant identifier and noise cancellation. This algorithm has been researched for performance enhancement of filtering. The design and development of a reliable system has been becoming a key issue in industry field because the reliability of a system is considered as an important factor to perform the system's function successfully. And the computing with reliability and fault tolerance is a important factor in the case of aviation and nuclear plant. This paper presents design of reliable adaptive filter with fault tolerance. Generally, redundancy is used for reliability. In this case it needs computing or circuit for voting mechanism or computing for fault detection or switching part. But this presented Filter is not in need of computing for voting mechanism, or fault detection. Therefore it has simple computing, and practicality for application. And in this paper, reliability of adaptive filter is analyzed. The effectiveness of the proposed adaptive filter is demonstrated to the case studies of plant identifier and noise cancellation by using DSP.

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Mirrors and Optomechanical Structures Design and Analysis for Linear Astigma-tism Free Three Mirror System (LAF-TMS)

  • Park, Woojin;Hammar, Arvid;Lee, Sunwoo;Chang, Seunghyuk;Kim, Sanghyuk;Jeong, Byeongjoon;Kim, Geon Hee;Kim, Daewook;Pak, Soojong
    • The Bulletin of The Korean Astronomical Society
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    • v.43 no.2
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    • pp.55.4-56
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    • 2018
  • Linear Astigmatism Free - Three Mirror System (LAF-TMS) is the linear astigmatism free off-axis wide field telescope with D = 150 mm, F/3.3, and $FOV=5.51^{\circ}{\times}4.13^{\circ}$. We report the design and analysis results of its mirrors and optomechanical structures. Tolerance allowance has been analyzed to the minimum mechanical tolerance of ${\pm}0.05mm$ that is reasonable tolerance for fabrication and optical alignment. The aluminum mirrors are designed with mounting flexure features for the strain-free mounting. From Finite Element Analysis (FEA) results of mounting torque and self-weight, we expect 33 - 80 nm RMS mirror surface deformations. Shims and the L-bracket are mounted between mirrors and the mirror mount for optical alignment. The mirror mount is designed with four light-weighted mechanical parts. It can stably and accurately fix mirrors, and it also suppresses some of stray light.

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Relationship between Drought-Tolerance and Physiological Parameters in Korean Barley Genotypes (보리 품종의 한발저항성과 생리적 지표와의 상관)

  • 이변우;부금동;백남천;김정곤
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.48 no.6
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    • pp.516-526
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    • 2003
  • Thirty-six barley varieties including Korean modern and local varieties were tested for drought-tolerance in the field of plastic rain shelter, Drought treatment was initiated at initial tillering stage (March 27, 2002) by withholding irrigation and lasted until harvest. Soil water potential maintained at around -0.05㎫ in the control plot and varied from -0.05㎫ (at the initial stage of drought treatment) to -0.29㎫ in the drought treatment plot. At forty days after drought treatment, relative water content (RWC), osmotic potential (OP), osmotic adjustment (OA), and $^{13}\textrm{C}$ discrimination ($\Delta$) were measured and then plants were sampled for leaf area index (LAI) and dry weight (DW). Barley was harvested at maturity for determining DW, grain yield, 1000 grains weight and number of spikelet. The tested varieties revealed wide spectrum of drought tolerance. Dongbori-1, Chalbori, Changyeongjaerae, Samdobori and Weolseong 87-31 showed strong drought-tolerance while Songhagbori and Suwonmaeg360 showed weak drought-tolerance. The drought injury indexes (drought/control ratio) of DW and yield revealed significant positive correlation with leaf RWC in drought treatment plot and $\Delta$ in the control plot, but obvious negative correlation with leaf OP and OA under drought condition. In addition, all the drought indexes of OP, $\Delta$ and RWC showed obvious positive correlation with the drought injury indexes of DW, 1000 grain weight and yield. Thus, OP and RWC under drought condition and $\Delta$ under well-watered condition would be used as the evaluation criteria for drought- tolerance of barley genotypes. However, further investigation is needed for the relationship between $\Delta$ and drought-tolerance as the other reports were not consistent with our result.

Roc10, a Rice HD-Zip transcription factor gene, modulates lignin biosynthesis for drought tolerance

  • Bang, Seung Woon;Lee, Dong-Keun;Jung, Harin;Chung, Pil Joong;Kim, Youn Shic;Choi, Yang Do;Suh, Joo-Won;Kim, Ju-Kon
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.159-159
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    • 2017
  • Drought, a common environmental constraint, induces a range of physiological, biochemical and molecular changes in plants, and can cause severe reductions in crop yield. Consequently, understanding the molecular mechanisms of drought tolerance is an important step towards crop biotechnology. Here, we report that the rice (Oryza sativa) homeodomain-leucine zipper class IV transcription factor gene, ${\underline{R}ice}$ ${\underline{o}utermost}$ ${\underline{c}ell-specific}$ gene 10 (Roc10), enhances drought tolerance and grain yield by increasing lignin accumulation in ground tissues. Overexpression of Roc10 in rice significantly increased drought tolerance at the vegetative stages of growth and promoted both more effective photosynthesis and a reduction in water loss rate, compared with non-transgenic controls or RNAi transgenic plants. Importantly, Roc10 overexpressing plants had a higher drought tolerance at the reproductive stage of growth and a higher grain yield compared with the controls under field-drought conditions. Roc10 is mainly expressed in outer cell layers including the epidermis and the vasculature of the shoots, which coincides with areas of cell wall lignification. Roc10 overexpression elevated the expression levels of lignin biosynthetic genes in shoots, with a concomitant increase in the accumulation of lignin, while the overexpression and RNAi lines showed opposite patterns of lignin accumulation. We identified downstream target genes of Roc10 by performing RNA-seq and chromatin immunoprecipitation (ChIP)-seq analyses of shoot tissues. Roc10 was found to directly bind to the promoter of PEROXIDASEN/PEROXIDASE38, a key gene in lignin biosynthesis. Together, our findings suggest that Roc10 confers drought stress tolerance by promoting lignin biosynthesis in ground tissues.

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