• Journal of Periodontal and Implant Science
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• v.52 no.3
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• pp.242-257
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• 2022

Purpose: This study investigated periodontal ligament (PDL) restoration in osseointegrated implants using stem cells. Methods: Commercial pure titanium and zirconium oxide (zirconia) were coated with beta-tricalcium phosphate (β-TCP) using a long-pulse Nd:YAG laser (1,064 nm). Isolated bone marrow mesenchymal cells (BMMSCs) from rabbit tibia and femur, isolated PDL stem cells (PDLSCs) from the lower right incisor, and co-cultured BMMSCs and PDLSCs were tested for periostin markers using an immunofluorescent assay. Implants with 3D-engineered tissue were implanted into the lower right central incisors after extraction from rabbits. Forty implants (Ti or zirconia) were subdivided according to the duration of implantation (healing period: 45 or 90 days). Each subgroup (20 implants) was subdivided into 4 groups (without cells, PDLSC sheets, BMMSC sheets, and co-culture cell sheets). All groups underwent histological testing involving haematoxylin and eosin staining and immunohistochemistry, stereoscopic analysis to measure the PDL width, and field emission scanning electron microscopy (FESEM). The natural lower central incisors were used as controls. Results: The BMMSCs co-cultured with PDLSCs generated a well-formed PDL tissue that exhibited positive periostin expression. Histological analysis showed that the implantation of coated (Ti and zirconia) dental implants without a cell sheet resulted in a well-osseointegrated implant at both healing intervals, which was confirmed with FESEM analysis and negative periostin expression. The mesenchymal tissue structured from PDLSCs only or co-cultured (BMMSCs and PDLSCs) could form a natural periodontal tissue with no significant difference between Ti and zirconia implants, consequently forming a biohybrid dental implant. Green fluorescence for periostin was clearly detected around the biohybrid implants after 45 and 90 days. FESEM showed the invasion of PDL-like fibres perpendicular to the cementum of the bio-hybrid implants. Conclusions: β-TCP-coated (Ti and zirconia) implants generated periodontal tissue and formed biohybrid implants when mesenchymal-tissue-layered cell sheets were isolated from PDLSCs alone or co-cultured BMMSCs and PDLSCs.

• Composite Materials and Engineering
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• v.3 no.3
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• pp.201-220
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• 2021

In this study, potential of three machine learning techniques i.e., M5P, Support vector machines and Gaussian processes were evaluated to find the best algorithm for the prediction of flexural strength of concrete mix with steel fibre. The study comprises the comparison of results obtained from above-said techniques for given dataset. The dataset consists of 124 observations from past research studies and this dataset is randomly divided into two subsets namely training and testing datasets with (70-30)% proportion by weight. Cement, fine aggregates, coarse aggregates, water, super plasticizer/ high-range water reducer, steel fibre, fibre length and curing days were taken as input parameters whereas flexural strength of the concrete mix was taken as the output parameter. Performance of the techniques was checked by statistic evaluation parameters. Results show that the Gaussian process technique works better than other techniques with its minimum error bandwidth. Statistical analysis shows that the Gaussian process predicts better results with higher coefficient of correlation value (0.9138) and minimum mean absolute error (1.2954) and Root mean square error value (1.9672). Sensitivity analysis proves that steel fibre is the significant parameter among other parameters to predict the flexural strength of concrete mix. According to the shape of the fibre, the mixed type performs better for this data than the hooked shape of the steel fibre, which has a higher CC of 0.9649, which shows that the shape of fibers do effect the flexural strength of the concrete. However, the intricacy of the mixed fibres needs further investigations. For future mixes, the most favorable range for the increase in flexural strength of concrete mix found to be (1-3)%.

• Advances in concrete construction
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• v.14 no.5
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• pp.299-307
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• 2022

Cement-based matrixes have low tensile strength and negligible ductility. Adding fibres to these matrixes will improve their mechanical properties and make these composites suitable for structural applications. Post-cracking tensile strength of steel fibers-reinforced cementitious composite materials is directly related to the number of transverse fibers passing through the crack width and the pulling-out behavior of each of the fibers. Therefore, the exact recognition of the pullout behavior of single fibers is necessary to understand the uniaxial tensile and bending behavior of steel fiber-reinforced concrete. In this paper, an experimental study has been carried out on the pullout behavior of 3D (steel fibers with totally two hooks at both ends), 4D (steel fibers with a total of four hooks at both ends), and 5D (steel fibers with totally six hooks at both ends) in which the fibers have been located either perpendicular to the crack width or in an inclined manner. The pullout behavior of the mentioned steel fibers at an inclination angle of 0, 15, 30, 45, and 60 degrees and with embedded lengths of 10, 15, 20, 25, and 30 millimetres is studied in order to explore the simultaneous effect of the inclination angle of the fibers relative to the alongside loading and the embedded length of fibers on the pullout response in each case, including the maximal pullout force, the slip of the maximum point of pullout force, pullout energy, fiber rupture, and concrete matrix spalling. The results showed that the maximum pullout energy in 3D, 4D, and 5D steel fibers with different embedded lengths occurs at 0 to 30° inclination angles. In 5D fibers, maximum pullout energy occurs at a 30° angle with a 25 mm embedded length.

• Steel and Composite Structures
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• v.46 no.1
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• pp.75-92
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• 2023

The novel composite cold-formed steel (CFS)/engineered cementitious composites (ECC) beams have been recently presented. The new composite section exhibited superior structural performance as a flexural member, benefiting from the lightweight thin-walled CFS sections with improved buckling and torsional properties due to the restraints provided by thinlayered ECC. This paper investigated the shear performance of the new composite CFS/ECC section. Twenty-eight simply supported beams, with a shear span-to-depth ratio of 1.0, were assembled back-to-back and tested under a 3-point loading scheme. Bare CFS, composite CFS/ECC utilising ECC with Polyethylene fibres (PE-ECC), composite CFS/MOR, and CFS/HSC utilising high-strength mortar (MOR) and high-strength concrete (HSC) as replacements for PE-ECC were compared. Different failure modes were observed in tests: shear buckling modes in bare CFS sections, contact shear buckling modes in composite CFS/MOR and CFS/HSC sections, and shear yielding or block shear rupture in composite CFS/ECC sections. As a result, composite CFS/ECC sections showed up to 96.0% improvement in shear capacities over bare CFS, 28.0% improvement over composite CFS/MOR and 13.0% over composite CFS/HSC sections, although MOR and HSC were with higher compressive strength than PE-ECC. Finally, shear strength prediction formulae are proposed for the new composite sections after considering the contributions from the CFS and ECC components.

• Advances in concrete construction
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• v.14 no.3
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• pp.185-194
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• 2022

For producing cement and concrete, the construction field has been encouraged by the usage of industrial soil waste (or) secondary materials since it decreases the utilization of natural resources. Simultaneously, for ensuring the quality, the analyses of the strength along with durability properties of that sort of cement and concrete are required. The prediction of strength along with other properties of High-Performance Concrete (HPC) by optimization and machine learning algorithms are focused by already available research methods. However, an error and accuracy issue are possessed. Therefore, the Enhanced Deep Neural Network (EDNN) based strength along with durability prediction of HPC was utilized by this research method. Initially, the data is gathered in the proposed work. Then, the data's pre-processing is done by the elimination of missing data along with normalization. Next, from the pre-processed data, the features are extracted. Hence, the data input to the EDNN algorithm which predicts the strength along with durability properties of the specific mixing input designs. Using the Switched Multi-Objective Jellyfish Optimization (SMOJO) algorithm, the weight value is initialized in the EDNN. The Gaussian radial function is utilized as the activation function. The proposed EDNN's performance is examined with the already available algorithms in the experimental analysis. Based on the RMSE, MAE, MAPE, and R² metrics, the performance of the proposed EDNN is compared to the existing DNN, CNN, ANN, and SVM methods. Further, according to the metrices, the proposed EDNN performs better. Moreover, the effectiveness of proposed EDNN is examined based on the accuracy, precision, recall, and F-Measure metrics. With the already-existing algorithms i.e., JO, GWO, PSO, and GA, the fitness for the proposed SMOJO algorithm is also examined. The proposed SMOJO algorithm achieves a higher fitness value than the already available algorithm.

• Korean Journal of Optics and Photonics
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• v.35 no.2
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• pp.61-70
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• 2024

We demonstrate a laser power meter calibration system based on 12-diode laser sources coupled to single-mode fibres in a wavelength range from 400 to 1,600 nm. In our system, three laser power controllers ensure that the output power uncertainty of all laser sources is less than 0.1% (k=2). In addition, all laser beams are adjusted to have similar beam sizes of approximately 2 mm (1/e²-width) at the measurement position to minimise unmeasured laser power on a detector. As a reference detector, we use an integrating sphere combined with silicon and indium gallium arsenide photodiodes to minimise the non-uniformity and non-linearity of responsivity. The minimum uncertainty of the calibration system is estimated to be 1.1% (k=2) for most laser wavelengths.

• Applied Microscopy
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• v.31 no.4
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• pp.333-345
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• 2001

The mature sperms of three species of cephalopods (Octopus minar, Octopus ocellatus, Todarodes pacificus) were observed by electron microscopy. The results obtained are as follows: The sperm lengths of Octopus minor and Octopus ocellatus of octopods are long and they are about $390{\mu}m$ and $125\sim130{\mu}m$, respectively, but the sperm length of Todarodes pacificus is short and about $35{\mu}m$. The sperm of Octopus minor has a helical acrosome and a head bent a little like a banana while Octopus ocellatus of octopod has a twisted acrosome and a long rod-shaped head. A number of horizontal stripes are observed as a periodic structure in their subacrosome cavities and dense plugs are formed in the cavities of their heads. On the other hand, the acrosome of Todarodes pacificus is circular cap-shaped, and its head is long and oval. It is notable that two small cavities were observed in its basal acrosome. Juxtanuclear acrosomal materials of high electron density filled the subacrosomal cavity. In the middle piece of mature sperms of Octopus minor and Octopus ocellatus, the mitochondria form the mitochondrial sleeve, but the numbers of mitochondria differ between the species so that they are $11\sim12$ and $8\sim9$, respectively. Meanwhile, in the middle piece of mature sperms of Todarodes pacificus, the mitochondria are separated from the axoneme, forming a mitochondrial spur in which $10\sim13$ mitochondria and some electron dense materials concentrate. The axoneme of Octopus minor, Octopus ocellatus and Todarodes pacificus are of 9+2 type in common, surrounded by 9 coarse fibres. A number of glycogen were observed only in the axoneme of Todarodes pacificus. The coarse fibres were found as far as the main piece of sperm tail in Octopks minor and Todarodes pacificus, while to the end piece of sperm tail in Octopus ocellatus.

• Journal of Korean Tunnelling and Underground Space Association
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• v.20 no.2
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• pp.469-483
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• 2018

Steel bars have been widely used as the primary reinforcement for Precast Segmental Concrete Lining for TBM Tunnels. Previously, studies have been carried out to gauge the potential for steel fiber reinforcement to replace the use of steel bar reinforcements in the segmental lining to reduce the amount of the steel bar reinforcement. Steel fiber reinforcements have been investigated and widely applied to SFRC TBM linings to improve the constructability of SFRC TBM linings worldwide. However, the steel fiber reinforcement often caused punctures to the water membranes inside tunnel lining and had long-term durability deterioration issues caused by steel corrosion, as well as cosmetic problems. Therefore, this paper sought to gauge the potential of synthetic fiber reinforcements, which have proven to be very attractive substitutes for steel fiber reinforcements. This study analyzed the performance of both steel and synthetic fiber reinforcements in segmental linings and evaluated the applicability of the fiber reinforcements to the TBM Precast Concrete Segmental Linings of TBM tunnels. As a conclusion, this study demonstrates that the potential use of steel and synthetic fibers in various combination, can substitute the rebar reinforcement in the concrete mix for segmental concrete linings.

• Applied Microscopy
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• v.31 no.3
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• pp.223-233
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• 2001

The spermiogenesis of a Korean octopus, Octopus minor, inhabiting western of Korea Sea was observed by electron microscopy . The obtained results are as follows: The spermiogenesis of Octopus miner proceeds through four stages; early- , mid- , and late-spermatid, and mature sperm. An early spermatid is a spherical cell looking light due to the low electron density. The acrosome formed from Golgi complex of the upper nucleus looks dark due to the high electron density. The extra-nuclear rod (enr) stemming from proximal centriole is transformed from round shape into oval shape, elongating to the upper nucleus. In our observation, the axoneme was being formed from distal centriole, and the manchette composed of a number of microtubules is also found around nuclear membrane. In a mid-spermatid, chromatins in the nucleus contract shaping fine threads, and the manchette is also observed around nuclear membrane. Especially, the spherical acrosome is transformed into long oval one which is tinged with a number of horizontal stripes and has the middle electron density. In a late-spermatid, chromatins in the nucleus contract thick and short. Furthermore, the mitochondrial sleeve, in which the axoneme is surrounded with mitochondria, is observed at middle piece. The axoneme has a typical structure of 9+2 and around it, 9 coarse fibers are observed. Also in the acrosome cavity of mature sperm, horizontal striation is found. However, regularly spaced processes are peculiarly observed in there. A sperm is about 390 fm long, whose head is bent a little like a banana while the acrosome region is helical. In the middle piece of sperm, $11\sim12$ mitochondria are surrounding coarse fibers that reach the main piece of tail, while nothing but 9+2 structured axoneme is found in the end piece.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.6 no.1
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• pp.73-87
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• 1996

The mechanical properties of 2D ceramic composites fabricated bythe newly developed powder infiltration and subsequent multiple impregnation process were characterised by both 3-point flexure and tensile tests. These tests were performed with strain gauge and acoustic emission instrument. The woven fabric composites used for the test have the basic combinations of $Al_{2}$$O_{3}$ fabric/$Al_{2}$$O_{3}$ and SiC fabric (Tyranno)/SiC. Uniaxially aligned SiC fibre(Textron SCS-6)/SiC composites were also tested for comparison, The ultimate flexural strength and first-matrix cracking stress of SiC fabric/SiC composite with 73% of theoretical density were about 300 MPa and 77 MPa respectively. However, the ultimate tensile strengths of composite were generally one third of flexural strengths, and first-matrix cracking stress in a tension test was also much lower than the value obtained from flexure test. The lower mechanical properties measured by tension test were analysed quantitatively bythe differences in stressed volume using Weibull statistics. This showed that the ultimate strength and the firs-tmatrix cracking stress of woven laminate composites were mainly determined bythe gauge length of fibres and the stressed volume of matrix respectively. Incorporation of SiC whiskers into the matrix increased first-matrix cracking stress by increasing the matrix failure strain of composites.