• Title/Summary/Keyword: fiber-FISH

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Action of Autonomic Drugs on the Urinary Bladder of Ditrema temmincki Bleeker (망상어 (Ditrema temmincki Bleeker)의 방광(膀胱)에 대(對)한 자율신경제(自律神經劑)의 작용(作用))

  • Bae, Wan-Soo
    • The Korean Journal of Pharmacology
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    • v.5 no.2
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    • pp.105-114
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    • 1969
  • The author studied the action of autonomic drugs on the urinary bladder isolated from Ditrema temmincki Bleaker and the results obtained were summarized as follows: 1) The motility of the urinary bladder of the fish was stimulated by acetylcholine and physostigmine. The stimulating action of these drugs was antagonized by atropine. 2) The motility of the fish bladder was stimulated by epinephrine, nor-epinephrine and phenylephrine, but inhibited by isoproterenol. 3) The inhibitory effects of isoproterenol on the fish bladder was not affected by phenoxybenzamine, but blocked by propranolol. 4) The excitatory effects of phenylephrine on the fish bladder were blocked by phenoxybenzamine, but augmented by propranolol. 5) The excitatory effects of epinephrine and nor-epinephrine were reversed by phenoxybenzamine and augmented by propranolol. 6) The motility of the fish bladder pretreated with phenoxybenzamine and propranolol was not affected by isoproterenol, phenylephrine, epinephrine and nor-epinephrine. 7) It seemed that the bladder muscle of the fish had both alpha excitatory and beta inhibitory receptors. 8) The motility of the fish bladder was stimulated by nicotine and DMPP. The excitatory effects of these drugs were abolished by pretreatment with hexamethonium or atropine. 9) It is, therefore, concluded that there are ganglion cells furnished with cholinergic fiber in the bladder wall of the fish.

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Effects of Dietary Nitrogen Sources on Fiber Digestion and Ruminal Fluid Characteristics in Sheep Fed Wheat Straw

  • Tan, Z.-L.;Lu, D.-X.;Hu, M.;Niu, W.-Y.;Han, C.-Y.;Ren, X.-P.;Na, R.;Lin, S.-L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.10
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    • pp.1374-1382
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    • 2001
  • Fifteen Inner Mongolian wethers with permanent ruminal and duodenal cannulas were used to study the effects of dietary rumen-undegradable protein (RUP) to rumen-degradable protein (RDP) ratios or protein sources on fiber digestion in the gastrointestinal tract and ruminal fluid characteristics. Fiber digestion and ruminal fermentation were not affected (p>0.05) by dietary RUP to RDP ratios (from 1.54 to 0.72). Soybean meal supplementation improved ruminal digestion. Fish meal supplementation increased (p<0.05) the ruminal degradability of fiber. The different RUP to RDP ratios (from 1.54 to 0.72) did not influence (p>0.05) ruminal fluid pH, but there were differences (p<0.05) in ruminal fluid $NH_3-N$ concentration because of urea replacement. Soybean meal as a dietary protein source decreased (p<0.05) ruminal fluid pH and increased (p<0.05 or p<0.01) $NH_3-N$, acetate, propionate and butyrate concentrations in the rumen. Fish meal as a dietary protein source decreased (p<0.05 or p<0.01) ruminal $NH_3-N$ and acetate concentrations and increased (p<0.05) ruminal propionate concentration. It can be concluded that dietary protein sources have more significant effect on fiber digestion and ruminal fermentation than different dietary RUP to RDP ratios, when the dietary crude protein requirements of growing sheep are satisfied.

Comparison of Food Consumption Pattern , Nutrient and Dietary Fiber Intakes between Female College Students and Middle Aged Women in Korea

  • Lee, Kang-Hee;Chyun, Jong-Hee
    • Journal of Nutrition and Health
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    • v.30 no.9
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    • pp.1088-1094
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    • 1997
  • One hundred twenty healthy college students and housewives from urban middle income household were investigated in terms of food consumption patterns, nutrient intake and dietary fiber intake. Total food intake was slightly lower in college students(1059.9g/d) than in housewives(1160.g/d) . However, the ration of animal food to total food was considerably higher in college students(23.7%) than in housewives(16.8%). College students tended to consume more dairly products, meat & products, and cereals & grain products but less vegetables , fruits, fish and shellfish than housewives. Although nutrient intake was not much different (except for total calories, fats, and ascorbic acid), dietary fiber intake was slightly and significantly lower in college students(14.9g/d, 8.3g/1000kcal) than in housewives(16.9g/d, 103g/1000kcal) . That is partially due to college students irregular food habits and the change in their meal patterns from traditional to western diets. There was a significantly positive correlation between dietary fiber intake and thiamin, riboflavin, niacin and crude fiber intake.

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Feasibility Study of Fiber Reinforced Concrete Using Waste Fishing Net (폐어망을 이용한 섬유보강 콘트리트의 적용성 연구)

  • Kweon, Gichul;Kim, Heeyun
    • International Journal of Highway Engineering
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    • v.16 no.6
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    • pp.87-93
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    • 2014
  • PURPOSES : To evaluate the feasibility of cut waste fishing net as a reinforced fiber for concrete. METHODS : Strength characteristics of fiber reinforced concrete using waste fishing net were investigated. The cut waste fishing nets with 4~5cm length were putted into the soil-cement and cement concrete for pavement slab. RESULTS : Compression and tensile strength of fiber reinforced concrete using waste fishing net were increased. CONCLUSIONS : It was concluded that cut waste fishing net can be used as a reinforced fiber for cement concrete. However, sometimes using cut waste fishing net leads to decrease the strength; therefore, further researches are needed for real project.

A Study on the Dietary Fiber Intake and Iron Metabolism in Korean Female College Students (일부 여대생의 식이섬유 섭취와 철분대사에 관한 연구)

  • 승정자
    • Journal of Nutrition and Health
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    • v.30 no.2
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    • pp.147-154
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    • 1997
  • This study was conducted to evaluate dietary fiber intake, iron status, and their correlations in 50 female college students living in Seoul. The nutritional status was analyzed using 3-day dietary record, duplicated diet collection, and venous blood sampling. The mean values for age, height, weight, BMI, and blood pressure of the subjects were 23.2 years, 160.2cm, 53.9kg, 21.0kg/$m^2$, and 110.1/68.4mmHg, respectively. Daily intakes of enery, protein, fiber, crude fiber, iron, and heme iron were 1635.5㎉, 54.3g, 22.5g, 6.8g, 16.2mg, and 0.2mg, respectively. Fiver intake was positively correlate with energy, protein, carbohydrate, vitamin C, iron, and crude fiber intakes. Also, iron intake was positively correlated with energy, protein, lipid, carbohydrate, and vitamin C intakes. There was a significant correlation between heme iron and MFP(meat, fish, poultry) intakes. To exame the iron balance, iron intake and excretion were measured. Iron intake and excretions through urine and feces were 19.5mg, 8.5mg, and 1.6mg, respectively. Based on these iron retention and iron apparent absorbability were calculated as 9.4mg and 52.4%, respectively. There was no significant relationship between dietary fiber intake and iron status. However, there were significant positive correlation between fiber intake and urinary iron excretion.

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Observation of Muscle Structure and DSC Measurement of Collagen of the Cultured and Wild Red Sea Bream and Flounder. (양식 및 자연산 도미와 넙치 어육 중의 콜라겐 DSC 측정 및 근육 조직 관찰)

  • 이경희;이영순
    • Korean journal of food and cookery science
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    • v.17 no.6
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    • pp.549-554
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    • 2001
  • Thermal measurements were made for connective tissues of 5 different fish muscles by using a differential scanning calorimeter(DSC), and connective tissues between muscle fibers and the cross sections of muscle fibers were observed by a light microscope. Red sea bream(cultured and wild) and flounder(cultured, cultured with obosan and wild) were used in this study. It was found that the connective tissues of cultured and frozen fish muscle required less endothermic enthalpy and the endothermic peak temperature was lower than those of wild and fresh ones when they were shrunken and denatured. Therefore, it is likely that the former are more unstable to heat than the latter. The cultured flounder fed with obosan and wild flounder which contained more collagen than cultured flounder and the wild red sea bream showed clear connective tissues between fibers. The cross-section of cultured fish muscle fiber was larger than that of wild one. From these results, collagen content and thermal properties of collagen, cross section of muscle fibers seemed to contribute to the textural difference between wild and cultured fish.

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Localization of 5S and 25S rRNA Genes on Somatic and Meiotic Chromosomes in Capsicum Species of Chili Pepper

  • Kwon, Jin-Kyung;Kim, Byung-Dong
    • Molecules and Cells
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    • v.27 no.2
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    • pp.205-209
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    • 2009
  • The loci of the 5S and 45S rRNA genes were localized on chromosomes in five species of Capsicum, namely, annuum, chacoense, frutescens, baccatum, and chinense by FISH. The 5S rDNA was localized to the distal region of one chromosome in all species observed. The number of 45S rDNA loci varied among species; one in annuum, two in chacoense and frutescens, and chinense, and four in baccatum, with the exceptions that 'CM334' of annuum had three loci and 'tabasco' of frutescens gad one locus. 'CM334'-derived BAC clones, 384B09 and 365P05, were screened with 5S rDNA as a probe, and BACs 278M03 and 262A23 were screened with 25S rDNA as a probe. Both ends of these BAC clones were sequenced. FISH with these BAC probes on pachytenes from 'CM334' plant showed one 5S rDNA locus and three 45S rDNA loci, consistent with the patterns on the somatic chromosomes. The 5S rDNA probe was also applied on extended DNA fibers to reveal that its coverage measured as long as 0.439 Mb in the pepper genome. FISH techniques applied on somatic and meiotic chromosomes and fibers have been established for chili to provide valuable information about the copy number variation of 45S rDNA and the actual physical size of the 5S rDNA in chili.

Vertebral abnormality in Hatchery-reared Black Sea Bream (Acanthoparus schlegelii) fingerlings (인공종묘 생산 감성돔 (Acanthoparus schlegelii) 치어의 척추 변형에 관하여)

  • Park, Sung-Woo;No, Yun-San;Yu, Jin-Ha;Kim, Jin-Do
    • Journal of fish pathology
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    • v.21 no.2
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    • pp.139-148
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    • 2008
  • Vertebral deformity in hatchery-reared black sea bream (Acanthoparus schlegelii) fingerlings occurred. Deformed fish had a good appetite but no clinical signs were found except the vertebral abnormality and darkness around the dorsal skin of the deformed vertebra. As more than 90% of the hatchery-reared fish exhibited vertebral abnormality, the fingerlings could not be used for commercial seeds any more. No morphological change in the swim bladder was observed. Histopathological changes on the deformed vertebra, gill, liver, and spleen were observed to clarify the cause of the deformity. The vertebra were irregularly deformed upward with the shape of "V" in an alphabetic character causing the spinal cord and dorsal aorta suppressed. The diameters of the muscle fibers around the deformed vertebra, especially, in the dorsal part, were much smaller and more irregular than those in normal fish, and the gabs between the fiber bundles were enlarged. No evidence of inflammatory responses in the lateral musculature were found. On the basis of normal inflation of the swim bladder, cultural environments for growth of the fingerlings, and histopathological alterations in the muscle, vertebra and gills, it is suggested that high speed of water current in the culturing aquaria between 23 to 30 days after hatching was responsible for the development of vertebral abnormality.

Fish Meal Replacement by Cottonseed and Soybean Meal in Diets for Juvenile Olive Flounder, Paralichthys olivaceus

  • Pham, Minh-Anh;Lee, Kyeong-Jun;Lim, Se-Jin;Lee, Bong-Joo;Kim, Sung-Sam;Park, Young-Jun;Lee, Sang-Min
    • Journal of Aquaculture
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    • v.18 no.3
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    • pp.215-221
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    • 2005
  • This study was conducted to investigate the effects of dietary supplementation of cottonseed and soybean meal on growth performance of juvenile olive flounder (Paralichthys olivaceus). Nine hundred fish $(0.74{\pm}0.11g)$ in the early juvenile stage were randomly divided into 15 groups, and 3 groups were fed one of five isonitrogenous (56% CP) and isocaloric $(16.3\;MJ\;kg^{-1})$ diets replacing 0, 10, 20, 30, and 40% of fish meal protein by equal proportion (1:1, w:w) of cottonseed and soybean meal (CS) (designated by Control, CS10, CS20, CS30, and CS40, respectively). A solvent extracted cottonseed meal containing high crude protein (44%) and low fiber content (<12%) was used in this study. After 10 weeks of feeding trial, the growth offish fed diets CS10, CS20, and CS30 were not significantly (P>0.05) different compared to that of fish fed the control diet. However, diet CS40 exhibited significantly lower (P<0.05) growth performance than the control diet. No differences were observed in whole body composition of fish fed all the experimental diets. This study indicates that mixture of cottonseed and soybean meal with lysine and methionine supplementation can replace up to 30% fish meal protein in diet for olive founder at the early juvenile stage. However, we suggest that 20% of fish meal protein replacement by cottonseed (10%) and soybean (10%) meal can be the optimum level for commercial use in safety according to the growth performances.