• Applied Microscopy
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• v.41 no.1
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• pp.31-35
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• 2011

The fine structures of the sperm morphology in the Myotis daubentonii ussuriensis were observed by transmission electron microscope. The results showed that the sperm head revealed bullet shaped, the width was showed a slender more than toward the posterior region to anterior region of nucleus. The sperm head was about $4.5{\mu}m$ in length, being about $2.0{\mu}m$ in width. The nuclear length was $4.3{\mu}m$, occupied most of the sperm head. The nucleus and acrosome were separated by the apical body. The neck region was composed the basal plate, capitulum and segmented columns. The segmented columns were about 12 to 14 in number and connected with the outer dense fibers of the middle piece. The mitochondria sheath were arranged like the thread of a screw, and the total number of mitochondrial gyres were 57. The satellite fibers were observed irregularly among the outer dense fibers in the middle piece. Except the middle piece they are not observed in the principal and end pieces of the tail. In general, the tail show axoneme composed of a 9+2 microtubular pattern, and microtubules of the end piece were arranged irregularly.

• The Korean Journal of Zoology
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• v.33 no.3
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• pp.354-364
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• 1990

The ultrastructure and silk formational process of the flagelliform glands, one of the capture thread producing organs in the orb web spider (Nephila clavata L. Koch), have been studied with electron microscope. A pair of flagelliform glands consist of three distinct regions: excretory duct, storage sac and convoluted tail. This gland is connected to the large spinning tube (spigot) of the posterior spinnerets with two pairs of aggregate glands, and showed a characteristic spigot structure called "triad". The excretory duct consists of three long segments surrounded by the same sheath of connective tissue. The distal duct near the spinning tube bears the electron lucent subcuticles which have the functions of water removal and orientation of the silk fiber, whereas the proximal duct near the sac contains the cuticule precursor transported through the intercellular space. Cytoplasms of the sac and tail regions have well developed rough endoplasmic reticulum and contain the round secretory granules commonly, but the electron densities and internal textures of the granules do not coincided. Adjacent epithelial cells are connected with the specialized septate junctions, and secretory granules accumulated at the apical pole are released to the lumen by the apocrine secretion.secretion.

• Korean Journal of Veterinary Research
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• v.37 no.1
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• pp.59-69
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• 1997

The histological and histochemical study of the body of axis and 1st intervertebral disc in the Korean native cattle fetus was attempted to early developmental process. The experimental animals used in this study were collected from fetus of the Korean native cattle ranging from 50mm to 180mm in Crown-Rump length(CR-length). The results were summerized as follows: 1. The ossification centers appeared centrum 1 and centrum 2 of the axis in 110mm CR-length fetus of the Korean native cattle. The centrum 2 was formed little earlier than the centrum 1. 2. The histochemical reactions for collagenous fibers in the axis revealed negative in 50mm CR-length, mild positive in 90mm CR-length, and strong positive in 110mm CR-length, respectively. 3. Dense collagenous fibers were observed in the notochord through the centrum 2, and intervertebral disc developed into cuneiform dorsoventrally, It's contour looks like an annual ring. These fiber bundle arranged lamellar formation. 4. The intervertabral disc of 50mm CR-length fetus was composed mainly mesenchymal cells, and these cell showed aggregation in the central portion. The intervertebral disc of 110mm CR-length fetus was consisted of pricipally fibroblast, and notochordal sheath formed with two layers in the center.

• Journal of the Korean Geotechnical Society
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• v.19 no.2
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• pp.279-289
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• 2003

Geosynthetic reinforced earth wall was introduced about 20 years ago and many structures have been constructed. Especially, segmental concrete panel facing and friction tie system are the most popular system in Korea, and this friction tie was composed of high tenacity PET filament and LDPE(Low Density Polyethylene) sheath. Due to the lack of direct-test results, design coefficients of friction tie (creep reduction factor) had been determined by quoting the previous and the foreign reference data. This is an unreasonable fact for the use of friction ties. In this study, the creep tests were performed to evaluate the creep behavior of friction tie, and the reduction factor of creep was calculated for the correct design of geosynthetic reinforced earth retaining walls. From the test results, finally it was found that the allowable creep strength of friction tie is 60% of Tult during service life, and creep reduction factor is 1.67 for each grade of friction ties.

• Archives of Plastic Surgery
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• v.32 no.5
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• pp.613-618
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• 2005

When a large peripheral nerve defect occurs, an autologous nerve graft is the most ideal method of recinstruction. But an autologous nerve graft has many limitations due to donor site morbidities. Many previous focused on finding the ideal nerve conduit. Among them, $Gore-Tex^{(R)}$ has several advantages over other conduits. It can be manipulated to a suitable size, does not collapse easily, and it is a semi- permeable material that contain pores. A round shaped nerve can be newly formed because of its smooth inner surface. The purpose of this study was to evaluate the availability of $Gore-Tex^{(R)}$ tube as a nerve conduit at the peripheral nerve defect in the rat sciatic nerve. The 10 mm nerve gap was made in each group. A $Gore-Tex^{(R)}$ tube filled with skeletal muscle was inserted and autologous nerve graft was harvested, respectively. In the experimental group, we placed a 0.5 mm thickness, $30{\mu}m$ pored, 1.8 mm in diameter and 14 mm length tube with skeletal muscle inserted inside. In the control group, the nerve gap was inserted with a rat sciatic nerve. We estimated the results electrophysiologically and histologically to 16 weeks postoperatively. Results in the nerve conduction velocity, total myelinated axon count, myelin sheath thickness and mean nerve fiber diameter, the experimental group was substantially lower than that of the control group, but the statistic difference was not significant (p<0.05). The morphology was very similar in both groups, microscopically. From the above results, We conclude that $Gore-Tex^{(R)}$ qualifies as an ideal nerve conduit. It is suggested that $Gore-Tex^{(R)}$ tube filled with skeletal muscle may, substitute for an autologous nerve graft.

• Journal of Veterinary Science
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• v.19 no.6
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• pp.750-758
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• 2018

Influenza virus infection is a zoonosis that has great socioeconomic effects worldwide. Influenza infection induces respiratory symptoms, while the influenza virus can infect brain and leave central nervous system sequelae. As children are more vulnerable to infection, they are at risk of long-term neurological effects once their brains are infected. We previously demonstrated that functional changes in hippocampal neurons were observed in mice recovered from neonatal influenza infection. In this study, we investigated changes in myelination properties that could affect neural dysfunction. Mice were infected with the influenza virus on postnatal day 5. Tissues were harvested from recovered mice 21-days post-infection. The expression levels for myelin basic protein (MBP) were determined, and immunohistochemical staining and transmission electron microscopy were performed. Real-time polymerase chain reaction and Western blot analyses showed that mRNA and protein expressions increased in the hippocampus and cerebellum of recovered mice. Increased MBP-staining signal was observed in the recovered mouse brain. By calculating the relative thickness of myelin sheath in relation to nerve fiber diameter (G-ratio) from electron photomicrographs, an increased G-ratio was observed in both the hippocampus and cerebellum of recovered mice. Influenza infection in oligodendrocyte-enriched primary brain cell cultures showed that proinflammatory cytokines may induce MBP upregulation. These results suggested that increased MBP expression could be a compensatory change related to hypomyelination, which may underlie neural dysfunction in recovered mice. In summary, the present results demonstrate that influenza infection during the neonatal period affects myelination and further induces functional changes in influenza-recovered mouse brain.

• Journal of the Korean Wood Science and Technology
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• v.46 no.3
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• pp.231-240
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• 2018

Bamboo is one of the major biomass resources that have many advantages such as fast growing, easy cultivation, short rotation, and a relatively lower price. In this study, the anatomical characteristics of Moso bamboo (Phyllostachys pubescens) by age from one-year-old to five-year-old were examined by optical and scanning electron microscopy. Also, the crystalline properties such as relative crystallinity and crystallite width were investigated by an X-ray diffraction method. In one-year-old bamboo xylem, a few vascular bundles showed missing bundle sheath in near the intercellular space. Moreover, one-year-old bamboo had the shortest fiber length and the smallest values in vessel diameter, width of vascular bundle, and thickness of inner layer. One-year-old bamboo also showed the smallest values in the crystallinity and crystallite width. Near epidermis samples had longer fiber length and greater vascular bundle spacing than near pith samples. Relative crystallinity and crystallite width near the epidermis were also higher than near the pith. This study revealed significant differences in qualitative and quantitative anatomical characteristics between one-year-old and two-year- or more old Moso bamboos. It is concluded that the structural differences by bamboo age can be used to differentiate the characteristics of juvenile and mature bamboo.

• Journal of The Korean Society of Grassland and Forage Science
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• v.11 no.2
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• pp.121-128
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• 1991

Forage productivity of two sorghum hybrids, Pioneer(P) 931 and P 956 and a sorghum-sudangrass hybrid, P 988, was studied. The 1st-cut of sorghun hybrids was made for silage 6 times from heading stage at a week-interval and regrowth (2nd-cut) was harvested on Oct. 13 although plants were not reached the same growth stage of 1st-cut. However, the 1st- and 2nd-cut of sorghum-sudangrass were made for green fodder 6 times from 4 weeks before heading stage at a week-interval. 1. Percent dry matter(DM) of 1st-cut of sorghum and 1st- and 2nd-cut of sorghum-sudangrass increased as growth stage advanced. Percent DM of 2nd-cut of sorghum decreased as the 1st-cut date delayed due to insufficient growing period on Oct. 13. 2. In the 1st-cut of sorghum the proportion of leaf blade (LB) or culm+leaf sheath (LS) to total DM decreased, but that of panicle increased as growth stage advanced. The panicle proportion of P 956 increased and culm+LS decreased more rapidly compared with those of P 931. IIowever, in sorghum-sudangrass hybrid the proportion of LB decreased and culmf LS increased as growth stage advanced through heading stage. 3. In the 1st-cut of sorghum hybrids crude(C) protein and C. fiber decreased, but nitrogen free extract (NFE) increased as growth stage advanced although C. fat and C. ash were similar among the growth stages. In the 2nd-cut of sorghum C. protein and C. ash increased, but NFE decreased as harvesting date delayed due to plants were younger although C. fat and C. fiber were similar among the harvesting dates. In the sorghum-sudangrass C. protein of 1st-cut decreased, but C. fiber of 1st-cut and NFE of the 1st-and 2nd-cut increased as growth stage advanced. 4. DM yield of 1st-cut of sorghum increased from heading stage to 3 weeks after heading and then levelled off, but total DM including regrowth was similar among the harvesting time. In the sorghum-sudangrass hybrid DM yield of 1st-cut and total DM yield including 2nd-and 3rd-cut increased as the harvestingtime delayed.

• The Korean Journal of Pain
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• v.12 no.2
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• pp.177-182
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• 1999

Background: Clonidine, an ${\alpha}_2$ adrenergic agonist blocks nerve conduction. However, in our previous experiment we found that adrenaline neither blocks nerve conduction by itself nor augment nerve conduction blockade by lidocaine near clinical concentrations. Possible explanations are: 1) there may be antagonism between some of adrenergic receptors, 2) clonidine may block nerve conduction via non-adrenergic mechanism. The purpose of this study is to obtain dose-response curves of several different forms of adrenergic receptor agonist to see the relative potencies of each adrenergic receptors to block nerve conduction. Methods: Recordings of compound action potentials of A-fiber components (A-CAPs) were obtained from isolated sciatic nerves of adult male Sprague-Dawley rats. Nerve sheath of the sciatic nerve was removed and desheathed nerve bundle was mounted on a recording chamber. Single pulse stimuli (0.5 msec, supramaximal stimuli) were repeatedly applied (2Hz) to one end of the nerve and recordings of A-CAPs were made on the other end of the nerve. Dose-response curves of epinephrine, phenylephrine, isoproterenol, clonidine were obtained. Results: $ED_{50}$ of each adrenergic agonist was: $4.51\times10^{-2}$ M for epinephrine; phenylephrine, $7.74\times10^{-2}$ M; isoproterenol, $9.61\times10^{-2}$ M; clonidine, $1.57\times10^{-3}$ M. Conclusion: This study showed that only clonidine, ${\alpha}_2$ adrenergic agonist, showed some nerve blocking action while other adrenergic agonists showed similar poor degree of nerve blockade. This data suggest that non-effectiveness of epinephrine in blocking nerve conduction is not from the antagonism between adrenergic receptors.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.33 no.3
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• pp.229-235
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• 1988

Several agronomic characters, feed value, and mineral uptake of two corn hybrids infected by different degrees of rice black-streaked dwarf virus (RBSDV) were studied at the south-eastern part of RBSDV prevalent areas in Korea. Although both hybrids were infected with RBSDV by 100%. culm length of Suweon 19 was more severely reduced than that of Jinjuok and there were positive correlations between culm length and top dry weight of individual plants. The reduced culm length was largely due to the shortened length of upper five internodes in both hybrids and also due to the reduced number of internodes in Jinjuok. As culm length reduced, percent ear-bearing plants, average ear weight, and number of kernels per ear reduced significantly in both hybrids. The kernel weight of Suweon 19 decreased with reduced culm length, but that of Jinjuok was similar among the different culm lengths. However, as culm length decreased, crude protein and fiber in whole plants and N, P, K, Mg in culm ＋ leaf sheath increased, but nitrogen free extract in whole plant decreased. And crude fat in whole plant and N, P, K, Mg concentrations in leaf blades and ear were similar among the culm lengths.