• 한국초지조사료학회지
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• 제17권1호
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• pp.75-81
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• 1997

This study was wnducte to investigate the effects of treatment level and seasons of sluny hm bovine feces on the productivity of rye, N efficiency and improvement of soil fertility under the Korean climate condition. The results obtained fiom this study summarized as follows ; 1. The highest dry matter yield of rye was obtained in the partial fertilization of sluny in spring or autumn. There is no differences of dry matter yield between spring and autumn application. 2. With increasing the amount of slurry-N, the dry matter yield of rye was signigicantly increased up to 100Kg sluny Nha. As the level of slurry-N rises above about 100Kg N/ha, the maximal yield of dry matter was unchanged or declined. 3. As the level of sluny fertilization rises, the crude protein content of rye increases significantly. However the contents of crude protein was less affected by the application seasons. 4. The amount of nitrogen which produced 6om rye is dependent upon the level of slurry-N. The highest nitrogen yield of rye was obtained by the partial fertilization of sluny-N. 5. The season or amount of slurry treatments did not affect the organic matter content in soil. N-content in soil was the lowest by the partial fertilization of slurry in spring or autumn. However, N-content was increased with the higher level of sluny-N.

• 한국가축번식학회지
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• 제13권3호
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• pp.171-178
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• 1989

These experiments were carried out to investigate the effect of a co-culture with granulosa cells on in vitro maturation, fertilization and development of bovine follicular oocytes. The bovine ovaries were obtained at a slaughter house and the follicular oocytes were recovered by aspirating the follicular fluid from the visible follicles of diameter 2-6mm. Bovine oocytes were matured in vitro for 24-26 hr and then fertilized in vitro using epididymal spermatozoa capacitated by preincubation for 2-3hr in BO solution containing BSA(5mg/ml) and caffein(25mM). Eight hours after insemination, the oocytes were cultured in a co-culture system with granulosa cells. The rates of maturation of the follicular oocytes cultured in a co-culture system with granulosa cells were 83.1%, the rate of fertilization of the follicular oocytes culture in a co-culture in a co-culture system with granulosa cells were 76.9%, respectively. No significant difference are observed between control and treatment in maturation and fertilization rates. The rates of embryos developed to 2-, 4-, 8-, 16-cell and monula stages after co-cultured with granulosa cells were 65.8, 57.9, 39.5, 34.2 and 34.2%, respectively. The value for 16-and morula stages were significantly higher (P<0.05) than those of the embryos cultured in the basic medium.

• Clinical and Experimental Reproductive Medicine
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• 제24권1호
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• pp.83-93
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• 1997

Although IVF-ET is widely applied in the treatment of couples with male factor infertility, it may fail in many infertile couples with normal semen parameters, and certain couples cannot be accepted for standard IVF-ET due to unfertilization or extremely low fertilization rate of oocytes. Recently, several procedures of microassisted fertilization (MAF) using micromanipulation have been introduced, and pregnancies and births have been obtained after partial zona dissection (PZD), subzonal insertion (SUZI), and intracytoplasmic sperm injection (ICSI). This clinical study was performed to develop and establish ICSI as an effective procedure of MAF in infertile couples who could not undergo standard IVF-ET repetitively because of failure in fertilization or extremely low fertilization rate of oocytes with the conventional fertilization technique in the previous IVF-ET cycles. From March, 1995 to May, 1996, 27 cycles of IVF-ET with ICSI in 19 infertile patients were included in study group, and the outcomes of ICSI were analyzed according to fertilization rate, cumulative embryo score (CES), and pregnancy rate. The number of oocytes retrieved after controlled ovarian hyperstimulation (COH) was $10.50{\pm}6.13$ in 30 previous cycles, and $10.57{\pm}5.53$ in 27 ICSI cycles. In ICSI cycles, the number of oocytes optimal for ICSI procedure was $7.89{\pm}4.30$, and the fertilization rate of $67.9{\pm}20.2%$ could be obtained after ICSI. The number of embryos transferred was $1.43{\pm}2.40$ in previous cycles, and $4.36{\pm}1.77$ with the mean CES of $41.8{\pm}27.4$ in ICSI cycles. In ICSI cycles, the overall pregnancy rate was 29.6% (8/27) per cycle and 42.1% (8/19) per patient with the clinical pregnancy rate of 22.2% (6/27) per cycle and 31.6% (6/19) per patient. In conclusion, MAF of human oocytes with ICSI is a promising fertilization method for IVF-ET patients, especially with the past history of failure in fertilization or low fertilization rate of oocytes in the previous IVF-ET cycles, and ICSI using micromanipulation procedures applied to human oocytes will provide a range of novel techniques which may dramatically improve the pregnancy rate in IVF-ET program and contribute much to effective management of infertile couples.

• 화훼연구
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• 제17권3호
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• pp.147-151
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• 2009

본 실험은 스탠다드국화 'Baekma'품종의 줄기공동에 의한 줄기 부러짐을 방지하기 위하여 수행하였다. 'Baekma' 품종의 줄기공동은 초장이 20 cm정도 되었을 때부터 시작되어 지제부로부터 5 cm 사이에서는 공동화가 상당히 진전되어 있었고, 이 후 발뢰를 하여 초장이 80 cm 정도 되는 생육 후반기에는 상부 20 cm만 남기고 모두 공동이 발생하였다. 절회장과 경경, 꽃목길이는 정식거리 $11cm{\times}11cm$에서 가장 좋았고, 수광량은 정식거리 $9cm{\times}9cm$ 대비 정식거리가 $10cm{\times}10cm$, $11cm{\times}11cm$, $12cm{\times}12cm$로 넓어질수록 각각 47, 99, 143% 증가하였다. 줄기의 경도와 강도는 정식거리 $11cm{\times}11cm$에서 줄기강도 $567kgcm^{-2}$, 경도 $1,339kgcm^{-2}$로 가장 높았다. 액상 규산질 비료의 엽면시비시 절화장과 절화중은 1회 시비에서 가장 좋았고, 개화소요일수는 무처리에서 가장 짧았다. 규산질 비료의 엽면시비에 따른 줄기의 경도와 강도는 무처리에 비하여 시비구에서 높았으며, $60mg{\cdot}L^{-1}$, 1회 시비에서 경도와 강도가 가장 높았다. 줄기의 Si 함량은 무처리에 비하여 농도가 높아질수록 시비회수가 많을수록 Si의 함량이 높아지는 경향을 보였고, $30mg{\cdot}L^{-1}$의 농도로 2회 시비한 처리에서 무처리 대비 2배 이상 함량이 높았다.

• 한국산림과학회지
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• 제111권4호
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• pp.594-602
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• 2022

본 연구는 편백의 초기생장에 대한 slurry composting and biofiltration(SCB)액비의 효과를 분석하기 위해 대조구(control), 화학비료처리구(chemical fertilizer : CF), 퇴비처리구(compost : CP), 저농도액비처리구(low liquid fertilizer : LLF) 및 고농도액비처리구(high liquid fertilizer : HLF)로 조성하여 처리구별 5본씩 3반복 실험을 하였다. 생장량분석 결과, 고동노액비를 200% 처리한 HLF-200처리구에서 가장 높은 생장량을 보였다. 시비효과를 분석하기 위해 HLF-200처리구와 대조구를 대상으로 근원직경 및 수고 생장모델을 개발하였다. 그 결과, 대조구와 HLF-200처리구 근원직경생장모델의 경우 각각 Schumacher 동형방정식 및 Schumacher 다형방정식이 가장 적합한 것으로 나타났으며, 수고생장모델의 경우 Gompertz 다형방정식이 가장 적합한 것으로 나타났다. 결정된 모델에 따른 생장곡선을 통해 근원직경과 수고의 생장량 및 생장률 모두 대조구보다 HLF-200처리구가 높아 시비효과가 있는 것으로 나타났으며, SCB액비를 통한 시비처리가 편백의 초기생장 증진에 적합한 것으로 판단되었다.

• 한국농공학회논문집
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• 제55권3호
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• pp.91-104
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• 2013

The objectives of this study were to monitor and assess the environmental impacts of indirect wastewater reuse on water quality and soil in paddy fields. Yongin monitoring site (YI) irrigated from agricultural reservoir and Osan monitoring site (OS) irrigated with treated wastewater diluted with stream water were selected as control and treatment, respectively. Monitoring results for irrigation water quality showed a significant statistical difference in salinity, exchangeable cation and nutrients. Pond water quality showed a similar tendency with irrigation water except for the decreased difference in nutrients due to the fertilization impact. Soil chemical properties mainly influenced by fertilization activity such as T-N, T-P, and $P_2O_5$ were changed similarly in soil profiles of both monitoring sites, while the properties, EC, Ca, Mg, and Na, mainly effected by irrigation water quality showed a considerable change with time and soil depth in treatment plots. Heavy metal contents in paddy soil of both control and treatment did not exceed the soil contamination warning standards. This study could contribute to suggest the irrigation water quality standards and proper agricultural practices including fertilization for indirect wastewater reuse, although long-term monitoring is needed to get more scientific results.

• Journal of Applied Biological Chemistry
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• 제65권1호
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• pp.1-6
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• 2022

요소는 가장 보편적인 질소비료 중 하나인데 빠른 가수분해와 환경으로의 질소 손실로 인해 작물에 질소 이용효율이 낮다. 따라서 요소 비료에서 질소 방출을 제어하는 것이 중요하다. 본 연구에서는 토양의 요소가수분해효소를 억제하여 요소에서 과도한 질소 용출을 방지하기 위해 목초액을 처리하였다. 목초액의 유효성분은 목초액을 처리하지 않은 토양에 비해 암모늄태 질소의 추출량을 79.7% 감소시켜 토양에서 요소의 암모니아화를 억제한 것으로 나타났다. 목초액이 요소의 시비 효율에 미치는 영향을 평가하기 위해 요소와 목초액을 처리한 토양에서 배추(Brassica campestris var. Pekinensis)를 각각 포트와 포장에서 재배하였다. 목초액을 요소와 같이 처리했을 때 관행적으로 사용하는 요소량의 반을 사용하였으며 관행과 비교하여 유사한 배추 생체중을 보였다. 배추의 질소 함량은 목초액 처리구에서 낮아 배추가 질소를 효율적으로 사용한 것으로 판단된다. 따라서 요소를 목초액의 유효성분과 같이 처리하면 시비량과 시비 횟수를 감소시킬 수 있다.

• 한국환경농학회지
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• 제41권4호
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• pp.230-235
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• 2022

BACKGROUND: Ammonia gas emitted from nitrogen fertilizers applied in agricultural land is an environmental pollutant that catalyzes the formation of fine particulate matter (PM2.5). A significant portion (12-18%) of nitrogen fertilizer input for crop cultivation is emitted to the atmosphere as ammonia gas, a loss form of nitrogen fertilizer in agricultural land. The widely practiced method for fertilizer use in agricultural fields involves spraying the fertilizers on the surface of farmlands and mixing those with the soils through such means as rotary work. To test the potential reduction of ammonia emission by nitrogen fertilizers from the soil surface, we have added N, P, and K at 2 g each to the glass greenhouse soil, and the ammonia emission was analyzed. METHODS AND RESULTS: The treatment consisted of non-fertilization, surface spray (conventional fertilization), and soil depth spray at 10, 15, 20, 25, and 30 cm. Ammonia was collected using a self-manufactured vertical wind tunnel chamber, and it was quantified by the indophenol-blue method. As a result of analyzing ammonia emission after fertilizer treatments by soil depth, ammonia was emitted by the surface spray treatment immediately after spraying the fertilizer in the paddy soil, with no ammonia emission occurring at a soil depth of 10 cm to 30 cm. In the upland soil, ammonia was emitted by the surface spray treatment after 2 days of treatment, and there was no ammonia emission at a soil depth of 15 cm to 30 cm. Lettuce and Chinese cabbage treated with fertilizer at depths of 20 cm and 30 cm showed increases of fresh weight and nutrient and potassium contents. CONCLUSION(S): In conclusion, rather than the current fertilization method of spraying and mixing the fertilizers on the soil surface, deep placement of the nitrogen fertilizer in the soil at 10 cm or more in paddy fields and 15 cm or more in upland fields was considered as a better fertilization method to reduce ammonia emission.

• 한국산림과학회지
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• 제105권2호
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• pp.177-185
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• 2016

시비는 고정포지에서 연속적인 묘목 생산에 의해 발생할 수 있는 양분 부족을 예방하고 건전한 묘목 생산을 위해 필수적이다. 본 연구에서는 물푸레나무, 들메나무, 잣나무, 전나무 묘목을 대상으로 무처리인 대조구를 포함하여, 단일원소비료 질소(N $13.8g{\cdot}m^{-2}$), 인(P $6.1g{\cdot}m^{-2}$), 칼륨(K $7.5g{\cdot}m^{-2}$) 처리와 NPK복합비료 1x(N $6.9g{\cdot}m^{-2}$, P $3.05g{\cdot}m^{-2}$, K $3.75g{\cdot}m^{-2}$), 2x(1x의 2배량), 4x(1x의 4배량) 처리가 묘목의 생장과 양분 변화에 미치는 영향을 양분벡터분석법(Vector diagnosis method)으로 정량화하였다. 토양 pH는 시비량이 증가할수록 감소하는 경향을 보였으며, 치환성 칼슘과 마그네슘은 질소와 복합비료 처리에서 감소하였다. 물푸레나무와 들메나무는 질소와 복합비료 시비에서 수고와 근원경이 유의하게 증가하였고, 잣나무와 전나무는 처리에 따른 차이가 없었다. 물푸레나무와 들메나무는 대조구에 비해 복합비료 처리에서 약 2배의 물질량 증가를 보였다. 양분벡터반응은 수종과 시비처리에 따라 상이한 경향을 보였는데, 물푸레나무는 식물체 내 질소 농도와 함께 질소함량이 감소되는 "양분희석" 현상이 나타났고, 식물체 인과 칼륨은 4x처리에서 식물체 농도 변동 없이 함량이 증가하는 "양분최적" 현상을 보였다. 들메나무는 질소, 인, 칼륨의 단일원소비료 처리에서 건중량의 변화 없이 질소 함량을 감소시키는 "체내이동" 현상을 보였으며, 복합비료 처리는 정도는 다르지만 "양분희석" 현상을 보였다. 본 연구는 양묘과정에서 물푸레나무와 들메나무는 적절한 시비가 요구되지만, 잣나무와 전나무는 시비가 필수적이지 않음을 보여주고 있다.

• Reproductive and Developmental Biology
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• 제32권4호
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• pp.217-222
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• 2008

The techniques of IVM, IVF and IVC of canine oocytes may provide useful information for gamete salvage programs and the conservation of endangered canidae. This investigation has been made to determine the efficiency of in vitro maturation (IVM) as a basic experiment to study the development of canine oocytes after in vitro fertilization (IVF). The rate of oocytes developing to the Mil stage was higher in the hormone treated group (10 IU/ml hCG+eCG, 14.7%, p<0.05) than in the control group (0 IU/ml hCG+eCG, 10.0%). The monospermy and pronuclear rates of canine oocytes were investigated after caffeine treatment on IVF. Canine oocytes were fertilized in the Fert-TALP medium supplemented with 0, 10, 20 or 30 mM caffeine (Fert I, Fert II, Fert III or Fert IV, respectively). The highest pronuclear formation rate was obtained in the Fert I for 24 h IVF (6.7%, 6/89). Therefore, it is believed that unlike in other mammals, caffeine in canine IVF does not increase the efficiency of fertilization rate, and is not an important factor.