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Effect of Various Caffeine Concentrations and Fertilization Time in In Vitro Fertilization of Canine Oocytes  

Kim, Bong-Soo (School of Life Science and Biotechnology, Kyungpook National University)
Yoo, Dong-Hoon (School of Life Science and Biotechnology, Kyungpook National University)
Shin, Mi-Jung (School of Life Science and Biotechnology, Kyungpook National University)
Kim, Hae-Jung (School of Life Science and Biotechnology, Kyungpook National University)
Lee, Dong-Seok (School of Life Science and Biotechnology, Kyungpook National University)
Hyun, Byung-Hwa (Disease Model Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Sang-Gyu (School of Life Science and Biotechnology, Kyungpook National University)
Park, Young-Sik (Department of Animal Science and Biotechnology, College of Agriculture and Life Sciences, Kyungpook National University)
Ha, Ji-Hong (School of Life Science and Biotechnology, Kyungpook National University)
Ryoo, Zae-Young (School of Life Science and Biotechnology, Kyungpook National University)
Publication Information
Reproductive and Developmental Biology / v.32, no.4, 2008 , pp. 217-222 More about this Journal
Abstract
The techniques of IVM, IVF and IVC of canine oocytes may provide useful information for gamete salvage programs and the conservation of endangered canidae. This investigation has been made to determine the efficiency of in vitro maturation (IVM) as a basic experiment to study the development of canine oocytes after in vitro fertilization (IVF). The rate of oocytes developing to the Mil stage was higher in the hormone treated group (10 IU/ml hCG+eCG, 14.7%, p<0.05) than in the control group (0 IU/ml hCG+eCG, 10.0%). The monospermy and pronuclear rates of canine oocytes were investigated after caffeine treatment on IVF. Canine oocytes were fertilized in the Fert-TALP medium supplemented with 0, 10, 20 or 30 mM caffeine (Fert I, Fert II, Fert III or Fert IV, respectively). The highest pronuclear formation rate was obtained in the Fert I for 24 h IVF (6.7%, 6/89). Therefore, it is believed that unlike in other mammals, caffeine in canine IVF does not increase the efficiency of fertilization rate, and is not an important factor.
Keywords
Canine; Oocyte; In vitro maturation; In vitro fertilization; Sperm capacitation;
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