• Journal of Aquaculture
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• v.16 no.1
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• pp.37-43
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• 2003

The present study reports morphology and developmental pattern off siliquosa cultured in a laboratory condition. The zygote was spherical with a diameter of 85 ${\mu}{\textrm}{m}$. During development the polarized zygote divided horizontally and the lower daughter cell divided horizontally into 2 cells. The upper cell was divided repeatedly in horizontal and vertical directions to form a cylinder-like structure, which subsequently developed into secondary and tertiary dichotomous branches. Optimum temperature for zygote release and fertilization was 25C. Injury inflicted by slicing was cured by epidermal differentiation, and adventitious branches; the branches emerging from the pith cells, however, developed no rhizoid. Adventitious branch formation rate was over 88% in all plates supplemented with 0.5 mg/L IAA and peaked at 98% under 0.5 mg/L IAA plus 0.5-5.0 mg/L NAA treatment. NAA stimulated the differentiation of adventitious branches at a wide range of concentrations, while IAA, 2,4-D and kinetin exhibited dose-dependent stimulation.

• Proceedings of the Korean Society of Agricultural Engineers Conference
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• 2000.10a
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• pp.611-616
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• 2000

Effect of reclaimed sewage on the paddy rice culture was examined by field experiment for two consecutive years. The domestic sewage was treated by the comstructed wetland, and the effluent of the treatment wetland was used for irrigation water. Growth components and yields were compared against the CONTROL plot where conventional fertilization was applied. In general, addition of the reclaimed sewage irrigation which contained high nutrient concentration didn＇t affect paddy rice culture adversely, and even better growth and more yields were observed. Overall, reclaimed sewage irrigation was thought to be one of practical alternatives for irrigation water.

• Journal of Embryo Transfer
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• v.33 no.4
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• pp.313-319
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• 2018

The purpose of this study was to analyze whether FSH and LH hormone treatment directly or indirectly affect embryo development in embryonic development. To determine this, we compared the development of embryonic cells through the expression pattern of MMPs. As a result, 33.8% of blastocysts were formed in FSH added group, 20.8% in LH added group and 10% in FSH + LH added group. In addition, the activity of MMP-9 was highly detected in the FSH-added group, and the expression of Casp-3 was much lower than that of the other groups. These results suggest that the addition of FSH seems to increase the activity of MMP-9 in embryonic cells, and that LH, on the contrary, may activate MMP-2 activity. In addition, the expression level of MMP-2 in the FSH-added group was high in the Trophoblast cell group and in the LH-added group, the hormone ideal secretion might affect the development of the embryonic cell.

• Development and Reproduction
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• v.2 no.2
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• pp.213-221
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• 1998

Intracytoplasmic sperm injection (ICSI) has been widely used to treat couples with infertility due to severely impaired sperm charateristics and for whom conventional in-vitro fertilization (IVF) had failed. The extent to which the morphology of the oocyte at the light microscopy level is related to the results of ICSI vis controversial. In this study, oocytes from 44 patients were reviewed. The ICSI procedure was recorded through CCD camera. The oocytes were divided into five groups according to the presence of cytoplasmic inclusions, the width of perivitelline space (PVS), the presence of cell debris in PVS, the status of first polar body and the flexibility of oolemma. The results showed that the fertilization rate and embryonic development were not associated with the morphological criteria of oocyte. The degeneraton rate of oocytes after ICSI was significantly higher (P<0.001) in the oocytes whose membranes were broken at the moment of insertion (17.7%) than the oocytes whose membranes were broken by aspiration of cytoplasm (1.6%). More oocytes with cytoplasmic inclusions (48.4% vs. 25.1%, p<0.001), wide PVS (35.2% vs. 19.0%, p<0.001), or cell debris in PVS (53.3% vs. 38.4%, p<0.05) were observed in patients with female factor infertility compared to patients with male factor infertility. These results .suggest that the fertilization rate and embryonic development after ICSI are not correlated with oocyte morphology based on the presence of cytoplasmic inclusions, size of PVS, the presence of cell debris in PVS and the status of polar body. And the degeneration rate of oocytes after ICSI was associated with the flexibility of oolemma.

• Korean Journal of Soil Science and Fertilizer
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• v.14 no.1
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• pp.37-43
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• 1981

This pot experiment was conducted to find out the optimal fertilization ratio of the cations(K: Ca: Mg) in various soil conditions. Soil samples were collected from uncultivated mountains and hills where development for grass lands is under consideration. The optimum ratios were computed by the Homes systematic variation technique. The results were summarized as follows : 1. The optimum fertilization ratios of the cations (K : Ca : Mg) for the high yields of grass-clover mixed sward at various areas were obtained (Table 4) 2. The relatively high optimum fertilization ratios of K in Daegu and Taegwalryong, Ca in Unbong, and Mg in Cheju district gave the high yields of clover in mixed sward. They were positively related to the low contents of the above cations in the soils or the relatively low content ratio comparing with other cations. 3. The yields of clover in mixed sward were seriously affected by the changes in both the fertilization ratio and total concentration of cations comparing to those of the grass yields. As a consequence, total yields, quality, and botanical composition of the mixed sward were also affected. 4. The antagonis among the cations K, Ca, and Mg was known from the point of mineral contents of grass and clover. 5. The equivalent ratio K/(Ca+Mg) or grass was greatly increased by the range of 2.4 and 3.3 in the K-group treatment, which was higher than the critical level 2.2 of grass tetany, otherwise that. of clover by the range of 0.9 and 1.2. Therfore, it may be concluded that if the botanical composition of grass/legume sward is optimum, the incidence of grass tetany can be eliminated.

• The Journal of Korean Obstetrics and Gynecology
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• v.33 no.3
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• pp.60-79
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• 2020

Objectives: This review plans to assess the efficacy and effectiveness of oriental medicine for the treatment of Ovarian Hyperstimulation Syndrome (OHSS) through literature research and overview. Methods: Database searching was conducted to identify relevant randomized controlled trials (RCTs) on oriental medicine for the treatment of Ovarian Hyperstimulation Syndrome. Studies were searched from Journal of Korean Obstetrics and Gynecology, Korean Medical Database, Korean studies Information Service System, China National Knowledge Infrastructure, Cochrane library, PubMed and EmBase up to 21^st May, 2020. Results: Seventeen studies were finally selected. Fifteen studies intervened with oral Chinese herb medicine, two studies intervened with acupuncture and moxibustion. Nine studies concluded that intervention with oriental medicine significantly relieved OHSS symptoms. Three studies reporting ovary diameter, four studies reporting abdominal circumference and other four studies reporting pelvic effusion showed significant reduction compared to control groups. Six studies showed significantly shorter duration for hospitalization in intervention groups. Only one study showed significantly higher pregnancy rate. Factors related with vascular permeability and blood cell coagulation were significantly lowered in intervention groups in general. Conclusions: From seventeen studies, oriental medicine relieved OHSS symptoms and showed treatment effectiveness. Further strictly designed studies and long-term observed studies are needed to establish evidences.

• Development and Reproduction
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• v.21 no.2
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• pp.205-213
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• 2017

The aim of this study was to determine the effect of additional alpha-linolenic acid (ALA) supplementation during in vitro maturation (IVM) and culture (IVC) on nucleic maturation and embryo development of pigs. Cumulus-oocyte complexes (COCs) were incubated in IVM medium containing different concentration of ALA (25, 50 and $100{\mu}M$) for 44 h. After in vitro maturation, nuclear maturation of oocytes were evaluated by aceto-orcein stain. Mature oocytes with $50{\mu}M$ ALA were fertilized and cultured in IVC medium with ALA (25, 50 and $100{\mu}M$) during early-embryogenesis (48 hours after fertilization). Then, embryos were cultured with $25{\mu}M$ ALA during early embryogenesis and/or late embryogenesis (120 hours after early-embryogenesis). In results, oocyte maturation were significantly increased by $50{\mu}M$ ALA treatment groups compared with control groups (p<0.05). Treatment of $25{\mu}M$ ALA during early-embryogenesis enhanced cleavage rate of embryo compared with other groups (p<0.05), whereas formation and total cell number of blastocyst had no significant difference. Similarly, cleavage rate of embryos were increased by $25{\mu}M$ ALA supplement during early- or late-embryogenesis than ALA treatment both stage of embryogenesis (p<0.05), but did not influence to blastocyst formation. Interestingly, total cell number of blastocyst were enhanced in ALA treatment group during early-embryogenesis. These findings indicated that ALA supplement enhance the nuclear maturation of oocyte and embryo development, however, excessive ALA could negatively influence. Therefore, we suggest that ALA is used for improvement of in vitro production of mammalian embryo and further study regarding with functional mechanism of ALA is needed.

• Journal of Bio-Environment Control
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• v.26 no.1
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• pp.1-6
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• 2017

The paprika has emerged as one of the highest-income crops by increase in dimestic and export demand in the greenhouse crops. Nevertheless, there is no standard for fertigation in soil, because general culture system is soilless culture. This study was conducted to establish the optimum nitrogen and potassium application level for paprika fertigation. Four different levels of nitrogen and potassium were applied, treatment levels were 0.5, 1.0, 1.5, 2.0 times of pimiento fertilization recommendations based on soil testing. Experiment to instigate the optimum amounts of nitrogen and potassium were carried out in 2012 and 2013, respectively13. Nitrogen application : stem diameter of 0.5 times was significantly lower than other treatments, but stem length was not affected by nitrogen fiertigation levels. Number of fruit and yield of first fruiting group harvest were not significant difference. but those of the second fruiting group were decreased by increasing nitrogen level beyond 1.0 times treatment and were the lowest in 0.5 times treatment. Overall, the optimum level of nitrogen for fertigation was judged 1.0 times of pimiento fertilization recommendations based on soil testing. Potassium application : Growth was no signigicant trend except stem length. Number of locule, fresh thickness and sugar content were not significant difference. Number of fruit and yield were not significant difference at the first and second fruiting group harvest. But those were significant difference at third fruiting group harvest, maximum yield was obtained by 1.5 times fertigation level. The optimum level of potassium for fertigation was judged 1.5 times of pimiento fertilization recommendations based on soil testing.

• Journal of Pharmacopuncture
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• v.19 no.4
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• pp.319-328
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• 2016

Objectives: Advancements in nanotechnology have led to nanoparticle (NP) use in various fields of medicine. Although the potential of NPs is promising, the lack of documented evidence on the toxicological effects of NPs is concerning. A few studies have documented that homeopathy uses NPs. Unfortunately, very few sound scientific studies have explored the toxic effects of homeopathic drugs. Citing this lack of high-quality scientific evidence, regulatory agencies have been reluctant to endorse homeopathic treatment as an alternative or adjunct treatment. This study aimed to enhance our insight into the impact of commercially-available homeopathic drugs, to study the presence of NPs in those drugs and any deleterious effects they might have, and to determine the distribution pattern of NPs in zebrafish embryos (Danio rerio). Methods: Homeopathic dilutions were studied using high-resolution transmission electron microscopy with selected area electron diffraction (SAED). For the toxicity assessment on Zebrafish, embryos were exposed to a test solution from 4 - 6 hours post-fertilization, and embryos/larvae were assessed up to 5 days post-fertilization (dpf ) for viability and morphology. Toxicity was recorded in terms of mortality, hatching delay, phenotypic defects and metal accumulation. Around 5 dpf was found to be the optimum developmental stage for evaluation. Results: The present study aimed to conclusively prove the presence of NPs in all high dilutions of homeopathic drugs. Embryonic zebrafish were exposed to three homeopathic drugs with two potencies (30CH, 200CH) during early embryogenesis. The resulting morphological and cellular responses were observed. Exposure to these potencies produced no visibly significant malformations, pericardial edema, and mortality and no necrotic and apoptotic cellular death. Conclusion: Our findings clearly demonstrate that no toxic effects were observed for these three homeopathic drugs at the potencies and exposure times used in this study. The embryonic zebrafish model is recommended as a well-established method for rapidly assessing the toxicity of homeopathic drugs.

• Applied Biological Chemistry
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• v.46 no.2
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• pp.134-139
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• 2003

To establish the fertilization condition to increase the productivity of Chasanthemum boreale M. with high quality, the effects of three nutrients (N, P, K) on the yields and the effective components were investigated in the pot scale. NPK was applied by chemical fertilizers with $(N-P_2O_5,-K_2O=250-160-160\;kg/ha)$ as a main treatment, and NP $(N-P_2O_5,-K_2O=250-160-160\;kg/ha)$, NK $(N-K_2O =250-160\;kg/ha)$, and PK $(P_2O_5K_2O=160-160\;kg/ha)$ treatments were settled as comparison. Dry yields of C. boreale M. was increased significantly to 4 fold higher by nitrogen. Nitrogen increased apparently plant growth and inorganic nutrient uptake. In the flower, which is most useful and edible part as a herbal medicine, main amino acids were glutamic acid and aspartic acids, and the total content was increased significantly by three elements of application. In addition, the content of cumambrin A, which is known to have the effect of blood-pressure reduction, was increased source to 6.2 times by nitrogen higher than that in PK treatment. Potassium was more effective in biosynthesis of cumambrin A than phosphorus, but the biological pathway was not clear, still.