• 한국가축번식학회지
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• 제15권2호
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• pp.125-132
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• 1991

The studies on the carried out to investigate the effects of capacitation method of spermatozoa on the in vitro fertilization and cleavage rate of bovine follicular oocytes. The ovaries were obtained from slaughtered Korean native cows. The follicular oocytes surrounded with cumulus cells were recovered by aspirating follicular fluids from the visible of diameter 3~5mm. The follicular oocytes were cultured in TCM-199 medium containing hormones and FCS for 24~48hrs in an incubator with 5% CO2 in air at 38.5$^{\circ}C$ and then matured oocytes were again cultured for 12~18hrs with motile capacitated sperm by preincubation of mKRB, treatment of HIS(high strength ion), Ca-IA(Inophore A), BFF(bovine follicular fluids) and heparin. The results obtained in these experiments were summarized as follows : 1. The in vitro fertilizatin and cleavage rate offollicular oocytes fertilized with capacitated spermatozoas in BO solution by preincubation of mKRB, treatment of HIS, Ca-IA, BFF and heparin method were 53.1%, 33.9%, 50.8%, 48.1%, 58.8% and 28.1%, 17.7%, 26.2%, 22.8%, 32.8%, respectively. And the fertilization and cleavage rate of heparin method was of highest of all. 2. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solutin by both caffeine, BSA and heparin methods were 65.8%, 70.3% and 40.8%, 47.3%, respectively, and those rates were higher treatment of heparin＋BSA, heparin＋caffeine than treatment of heparin. 3. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoa in BO solution with heparin concentrations of 2, 5, 10, 20, 40$\mu\textrm{g}$/ml were 50.0%, 54.7%, 58.1%, 51.7% and 27.9%, 32.8%, 37.1%, 30.0%, respectively. And the fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution with 10$\mu\textrm{g}$/ml of heparin was the highest of all. 4. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution containing heparin with caffeine concentraton of 10, 20, 30, 40$\mu\textrm{g}$/ml were 71.4%, 74.3% and 70.6%, 70.0% and 45.7%, 47.3%, 44.1%, 41.4%, respectively. The fertilization and cleavage rate of spermatozoa fertilized in BO solution with caffeine and heparin together(70.3~74.3%) was higher than that of spermatozoa fertilized in BO solution with heparin(58.8%). 5. The in vitro fertilization and cleavage rate of follicular oocytes fertilized with capacitated spermatozoas in BO solution containing heparin with BSA concentration of 5, 10, 20, 30$\mu\textrm{g}$/ml were 63.6%, 62.9%, 66.7%, 60.3% and 44.1%, 43.5%, 48.5%, 42.7%, respectively. The fertilization and cleavage rate of spermatozoa fertilized in BO solution with BSA and heparin together(60.3~66.73%) was higher than that of spermatozoa fertilized in BO solution with heparin(58.8%).

• 한국토양비료학회지
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• 제49권6호
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• pp.677-681
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• 2016

Excessive nitrogen fertilization influences crop yields and quality as well as environmental pollution. In this study, yields, nitrogen use efficiency and free sugar contents of burdock (Arctium lappa L.) were evaluated at different levels of nitrogen fertilization. Nitrogen fertilizer was applied at 5 levels (0, 50, 100, 150, 200%) based on the conventional fertilization ($N=230kg\;ha^{-1}$), and phosphate and potassium fertilizer were treated by conventional P and K fertilization ($P_2O_5-K_2O=140-210kg\;ha^{-1}$) in all plots. The root yields of burdock were the highest in N 100~150% treatment plots. Nitrogen use efficiency and nitrogen recovery decreased from over N 150% treatment. Nitrogen uptake of root was greater than that of shoot in N 50~200% treatments. Fructose contents in root were inversely proportional to the level of nitrogen fertilization. As considering nitrogen recovery and root quality, economical burdock yield was obtained in N $230kg\;ha^{-1}$.

• Clinical and Experimental Reproductive Medicine
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• 제47권2호
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• pp.130-134
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• 2020

Objective: The aim of this study was to compare the effects of conventional insemination (in vitro fertilization [IVF]) and intracytoplasmic sperm injection (ICSI) on the fertilization, developmental competence, implantation potential, and clinical pregnancy rate of embryos derived from in vitro matured oocytes of patients with polycystic ovary syndrome (PCOS). Methods: A prospective study was carried out among 38 PCOS patients who had undergone In vitro maturation (IVM) treatment. In total, 828 immature oocytes were collected from 42 cycles and randomly assigned for insemination by IVF (416 oocytes) or ICSI (412 oocytes). After fertilization, the embryos were cultured until the blastocyst stage and single embryos were transferred after endometrial preparation and under ultrasound guidance. Results: No significant differences were found in the maturation rate (78.1% vs. 72.6% for IVF and ICSI insemination, respectively; p= 0.076), fertilization rate (59.4% vs. 66.9% for IVF and ICSI insemination, respectively; p= 0.063), or the formation of good-quality blastocysts (40.9% vs. 46.5% for IVF and ICSI insemination, respectively; p= 0.314). Implantation and clinical pregnancy also did not show significant differences. Conclusion: There was a comparable yield of in vitro matured oocytes derived from PCOS patients in terms of fertilization, blastocyst formation, implantation rate, and clinical pregnancy between IVF and ICSI insemination. These findings provide valuable insights for choosing assisted reproductive treatment in women with PCOS, as IVM offers promising outcomes and is less invasive and less costly.

• 한국약용작물학회지
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• 제16권3호
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• pp.144-149
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• 2008

Shoot weight of Orostachys japonicus should determine its profitability. The study was done to determine the effect of phosphorus and potassium fertilization (0, 5, 10, 20 kg $P_2O_5$ or $K_2O$ /1000 $m^2$) on its growth, morphological characters, and flowering. Night-break treatment of 2 hours at midnight were done since Aug. 25 when afterward samples were taken every 4 weeks to Nov. 16. Growth, morphological and flowering related characters were measured from each sample. Plant height, inflorescence length, number of leaves including bracts and stem diameter were increased with increased P and K levels. Leaf and bract, floret, shoot and total dry weights showed similar response to the above morphological characters although P fertilization more increased them than K fertilization. Florets were more formed with increased P and K levels but were not flowered due to the night-break treatment, meaning that P and K fertilization had little effect on anthesis of the florets formed.

• 대한한방부인과학회지
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• 제28권4호
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• pp.118-125
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• 2015

Objectives : The purpose of this study is to report the effects of herbal medicine and acupuncture on a triplets pregnant patient with hyperemesis gravidarum. Methods : A patient who was pregnant with triplets by in vitro fertilization (IVF) with hyperemesis gravidarum received treatment as an inpatient by herbal medicne and acupuncture. We evaluated the results of treatment by change of symptoms and visual analogue scale (VAS) about nausea. Results : After treatment, almost symptoms of hyperemesis gravidarum were improved. Vomiting and VAS of nausea were reduced and intaking was increased as treatment. Conclusions : This study suggests that Korean medicine mainly herbal medicine and acupuncture is effective on a triplets pregnant patient by IVF with hyperemesis gravidarum.

• Clinical and Experimental Reproductive Medicine
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• 제42권2호
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• pp.45-50
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• 2015

Objective: Artificial oocyte activation (AOA) is an effective method to avoid total fertilization failure in human in vitro fertilization-embryo transfer (IVF-ET) cycles. AOA performed using a calcium ionophore can induce calcium oscillation in oocytes and initiate the fertilization process. We evaluated the usefulness of AOA with a calcium ionophore in cases of total fertilization failure in previous cycles and in cases of severe male factor infertility patients with non-motile spermatozoa after pentoxifylline (PF) treatment. Methods: The present study describes 29 intracytoplasmic sperm injection (ICSI)-AOA cycles involving male factor infertility at Cheil General Hospital from January 2006 to June 2013. Patients were divided into two groups (control, n=480; AOA, n=29) depending on whether or not AOA using a calcium ionophore (A23187) was performed after testicular sperm extraction-ICSI (TESE-ICSI). The AOA group was further split into subgroups according to sperm motility after PF treatment: i.e., motile sperm-injected (n=12) and non-motile sperm-injected (n=17) groups (total n=29 cycles). Results: The good embryo rate (52.3% vs. 66.9%), pregnancy rate (20.7% vs. 52.1%), and delivery rate (10.3% vs. 40.8%) were lower in the PF/AOA group than in the control group. When evaluating the effects of restoration of sperm motility after PF treatment on clinical outcomes there was no difference in fertilization rate (66.6% vs. 64.7% in non-motile and motile sperm, respectively), pregnancy rate (17.6% vs. 33.3%), or delivery rate (5.9% vs. 16.7%) between the two groups. Conclusion: We suggest that oocyte activation is a useful method to ensure fertilization in TESE-ICSI cycles regardless of restoration of sperm motility after PF treatment. AOA may be useful in selected patients who have a low fertilization rate or total fertilization failure.

• 한국산림과학회지
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• 제112권2호
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• pp.136-144
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• 2023

본 연구는 헛개나무(Hovenia dulcis Thunb.) 묘목 생산을 위한 적정 시비수준을 밝히고자 수행되었다. 헛개나무 1년생 용기묘를 대상으로 6가지 시비수준(대조구, 0.5, 1.0, 1.5, 2.0, 2.5 g·L^-1)에 따른 용기묘의 간장, 근원경, 물질생산량, 묘목품질지수, 엽록소 함량 등을 조사하였다. 연구 결과, 헛개나무 용기묘는 시비수준이 높아짐에 따라 간장 및 근원경 생장과 건중량이 증가하였으나, 1.5 g·L^-1 이상의 처리구에서는 큰 차이가 없었으며, 간장 생장의 경우 2.5 g·L^-1 시비처리구에서 유의적으로 낮은 값을 나타냈다. 묘목품질지수는 1.5 g·L^-1 이상 처리시, 처리구간 유의적 차이가 없는 높은 값을 보였으며, 엽록소 함량은 1.5 g·L^-1 처리구에서 가장 높은 값을 나타냈다. 시비처리에 따른 환경적, 비용적 측면을 고려 하였을 때, 우량 헛개나무 용기묘 생산을 위하여 요구되는 적절한 시비수준은 1.5 g·L^-1이며, 최소한의 묘목규격을 충족시키기 위해서는 적어도 0.5 g·L^-1의 시비처리가 필요할 것으로 판단된다. 본 연구의 결과는 약용 및 밀원자원으로 이용되는 헛개나무의 효율적인 묘목 생산체계 구축 및 안정적인 묘목 공급을 위한 기초자료로 활용될 수 있을 것으로 기대된다.

• 한국환경농학회지
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• 제42권1호
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• pp.28-34
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• 2023

Nitrogen fertilizers applied to agricultural lands for crop cultivation can be volatilized as ammonia. The released ammonia can catalyze the formation of ultrafine dust (particulate matter, PM2.5), classified as a short-lived climate change pollutant, in the atmosphere. Currently, one of the prominent methods for fertilizer application in agricultural lands is soil surface application, which comprises spraying the fertilizers onto the soil surface, followed by mixing the fertilizers with the soil. Owing to the low nitrogen absorption rate of crops, when nitrogen fertilizers are applied in this manner, they can be lost from land surfaces through volatilization. Therefore, investigating a new fertilization method to reduce ammonia emissions and increase the fertilizer utilization efficiency of crops is necessary. In this study, to develop a method for reducing ammonia emissions from nitrogen fertilizers applied to soil surfaces, deep fertilization was conducted using a newly developed deep fertilization device, and ammonia emissions from barley, garlic, and onion fields were examined. Conventional fertilization (surface application) and deep fertilization (soil depth of 25 cm) were conducted for analysis. The fertilization rate was 100% of the standard fertilization rate used for barley, and deep fertilization of N, P, and K fertilizers was implemented. Ammonia emissions were collected using a wind tunnel chamber, and quantified subsequently susing the indole-phenol blue method. Ammonia emissions released from the basal fertilizer application persisted for approximately 58 d, beginning from approximately 3 d after fertilization in conventional treatments; however, ammonia was not released from deep fertilization. Moreover, barley, garlic, and onion yields were higher in the deep fertilization treatment than in the conventional fertilization treatment. In conclusion, a new fertilization method was identified as an alternative to the current approach of spraying fertilizers on the soil surface. This new method, which involves injecting nitrogen fertilizers at a soil depth of 25 cm, has the potential to reduce ammonia emissions and increase the yields of barley, garlic, and onion.

• 한국수정란이식학회지
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• 제26권2호
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• pp.117-122
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• 2011

In this study, we examined the effectiveness of in vitro fertilization of porcine immature oocytes on the embryo development of blastocysts or hatched blastocysts and the number of cells according to the in vitro fertilization conditions. In the in vitro fertilization of in vitro matured porcine oocytes, there were no significant differences between treatment groups regarding fertilization rate, blastocyst rate, and embryo development of hatched blastocysts according to the storage periods of liquid sperm of 24, 48, and 72 hours. The embryo development rate of hatched blastocysts after the fertilization according to different spermatozoa concentrations ($0.4{\times}10^5$, $1.2{\times}10^5$, and $3.6{\times}10^5$ cells/ml) showed the highest rate in the group with a spermatozoa concentration of $1.2{\times}10^5$ cells/ml; in particular, this rate was significantly higher than that in the $0.4{\times}10^5$ cells/ml group (p<0.05). The total number of blastocysts cells as well as trophectoderms (TE) that developed in each treatment group were also significantly higher in the $1.2{\times}10^5$ cells/ml group than in any other groups (p<0.05). In contrast, the embryo development rate of blastocysts according to different co-incubation periods of sperm and oocyte (1, 3, and 6 hr) was high in the 6-hour group; in particular, the rate was significantly higher than that of the I-hour group (p<0.05). Furthermore, the total number of oocytes cells and TEs that developed was significantly higher in the 6-hour group than any other group (p<0.05). In this study, the most effective treatment conditions for porcine embryo development and high cell number were found to be as follows: a sperm storage period of less than 72 hours, a spermatozoa concentration of $1.2{\times}10^5$ cells/ml, and a 6-hour co-incubation period for sperm and ooocyte.

• 한국축산시설환경학회지
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• 제18권sup호
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• pp.7-12
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• 2012

To prevent the inappropriate treatment of livestock manure and induce the correct circulation of them into farmland, systematic monitoring and management by the treatment and circulation stage of livestock manure are required. The purpose of this study was to apply ubiquitous information communictation technology (u-ICT) to a livestock waste management system for better treatment by utilizing ubiquitous computing technology in the livestock sector. Elements and levels of applicable u-ICT technology for efficient livestock manure management were derived by analyzing previous researches. In addition, a conceptual diagram of an integrated management system was suggested by analyzing the existing liquid fertilization process.