• 식물조직배양학회지
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• 제22권6호
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• pp.317-322
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• 1995

속간교잡에 의한 신품종 육성을 위한 기초 연구로서 유자와 탱자를 정역교잡한 후 배주배양하였고, 자웅배우체 형성 및 수정현상을 조사하였다. 속간교잡의 결과율은 유자$\times$탱자가 16.6%, 탱자$\times$유자는 11.7%이었다. 수분후 6주된 배주를 MT 기본배지에 Zeatin 0.5 mg/L에 NAA 1 또는 3.0 mg/L에 배양했을 때 callus형성율이 높았지만, 14~15주된 배주를 2,4-D 0.1 mg/L 또는 0.5 mg/L이 첨가된 배지에 배양했을 때는 식물체 유기가 잘 되었다. 20주된 배주배양시 발아율은 유자 $\times$ 탱자의 경우 54.5%, 행자 $\times$ 유자는 48.6%이었다. F$_1$식물의 삼엽형 출현율은 유자$\times$탱자는 56.7%, 탱자$\times$유자는 100%이었다. 유자와 행자의 염색체수는 모두 n=9 (2n=18)이었고 화분립의 크기는 각각 33.75 $\mu$, 25.0 $\mu$이었다. 유자와 탱자 배낭의 길이와 폭은 69.38~79.23 $\mu$, 27.50~38.56 $\mu$, 난세포의 길이와 폭은 17.50~41.50 $\mu$, 6.25~8.12 $\mu$이었다. 수분 후 72시간이면 수정이 끝났다.

• 농업과학연구
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• 제11권2호
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• pp.207-217
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• 1984

상수리나무, 신갈나무 및 굴참나무의 근원경(根元徑)과 묘고(苗高)의 계절적(季節的)인 생육과정(生育過程)을 토양수분(土壤水分)${\times}$시비(施肥)로 구성(構成)되는 몇가지 처리(處理)를 한 후(後)에 10일(日) 또는 15일간격(日間隔)으로 조사(調査)하였다. 토양수분(土壤水分)과 시비처리(施肥處理)는 시간(時間)이 지나면서 서로 다르게 묘목(苗木)의 생육(生育)에 영향(影響)을 주었다. 그 생장(生長)은 수종별(樹種別)로 다른 시기(時期)에 수분처리간(水分處理間), 시비처리간(施肥處理間) 및 상호작용(相互作用)에서 고도(高度)의 유의적(有意的) 차이(差異)를 나타냈다. 토양수분(土壤水分)의 영향(影響)은 시비(施肥)에서 보다 늦게 나타났으며 N 또는 N+P+K 시비처리(施肥處理)에서 더욱 현저(顯著)한 차이(差異)를 보여 주었다. 생장(生長)에 크게 영향(影響)을 주는 토양수분(土壤水分) potential의 한계(限界)는 모든 수종(樹種)에서 -3~-6bar안에 있는 것으로 추정(推定)되었다. 모든 수종(樹種)에서 N 또는 N+P+K의 처리(處理)와 P, K 또는 무처리간(無處理間)에 유의적(有意的)인 생육차이(生育差異)를 보였으며 처리(處理)의 효과(效果)는 건조구(乾燥區)보다 습구(濕區)에서 더 큰 차이(差異)를 나타냈다. 토양수분(土壤水分)과 시비(施肥)의 상호작용(相互作用)은 모든 처리(處理)에서 시간이 지나면서 서서히 나타났다. 처리(處理)에 따른 근원경(根元徑)의 생육반응(生育反應)에 대(對)한 통계학적(統計學的)인 차이(差異)는 묘고(苗高)에 비(比)해서 조기(早期)에 보여줬다. 대상수종(對象樹種)의 생육반응(生育反應)을 비교(比較)할 때 굴참나무와 신갈나무는 상수리나무에 비(比)하여 보다 내건성(耐乾性)인 것으로 볼수 있었다.

• 한국잠사곤충학회지
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• 제52권2호
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• pp.117-122
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• 2014

선행연구를 통하여, 형질전환 누에를 제작하는 원천기술을 개발하고 이를 통해 녹색 형광실크를 생산하는 형질전환 누에를 제작한 바 있다. 본 연구에서는 선행연구에서 개발된 녹색 형광실크 형질전환 누에와 국립농업과 학원이 보유하고 있는 누에 유전자원 중 유색견(絹) 누에품종을 이용하여 형질전환 누에와 유색견 누에품종 간 교차교배를 통한 잡종강세를 이용하여 고치 생산량 및 고치의 색채 등이 향상된 녹색 형광실크 형질전환 누에 1대 잡종(F1) 계통을 개발하였다. 본 연구를 통해 선발된 실용형질이 우수한 농가보급형 녹색 형광실크 형질전환 누에 1대 잡종(F1) 계통은 기존 형질전환 누에의 녹색 형광단백질 뿐 아니라 유색견 누에 특유의 천연 연록색도 함께 갖고 있어 별도의 염색이 필요 없는 차별화된 고급 패션의류나 벽지 등의 고품질 소재로 활용이 가능할 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권6호
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• pp.754-758
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• 2001

Bovine oocytes with compact and complete cumulus cells were cultured for up to 24h in TCM199 buffered with 25mmol/l hepes and supplemented with 10% FBS (fetal bovine serum), 1mg/ml $17{\beta}$-estradiol, 20IU/ml hCG(human chorionic gonadotropin). All of the oocytes were divided into at 6 groups depending upon incubation times (control, 0 hour, 6 hours, 12 hours, 16 hours, 18 hours). To all experimental media, $200{\mu}g/ml$ puromycin was added at different incubation times mentioned above. Following these culture times, in vitro insemination was conducted with frozen-thawed bovine spermatozoa in medium BO (Brackett and Oliphant medium for in vitro insemination) with $10{\mu}g/ml$ BSA(bovine serum albumin) and 10 mg/ml heparin added. After 22h culture, the oocytes were fixed with acetic alcohol solution and stained with orcein acetic solution to evaluate sperm nuclear progression. Addition of puromycin after 0, 6 and 12 h of culture resulted in near of oocyte maturation at the M1 stage. Contrariwise, puromycin addition after 12 h of culture led to restoration of nuclear progression to M2 stage. On the one hand, puromycin affected the synthesis of Cyclin B protein that may be involved in the oocyte maturation and sperm capacitation for in vitro fertilization. The present study suggests the participation of protein synthesis, cyclin B, in the oocyte development from M1 to M2 stages in vitro.

• 한국초지조사료학회지
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• 제17권4호
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• pp.371-378
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• 1997

The purpose of this research was to investigate the effect of P fertilization and cutting fkquency without N fertilizer on nutritive value and nitrogen fixation activity of alfalfa. A field experiment was conducted on the experimental farm of Kongju National University at Yesan from 1994 to 1996. The experimental design was a split plot design with two P levels as main plot and two cutting frequencies as the sub-plot. The results obtained are summarized as follows. Available phosphate, exchangeable Ca and Na in the soil were slightly increased with P fertilizer and basal treatment of lime. Average DM yield was 1.5 ton and 1.4 ton in second and third year. There was no significant difference within P level and cutting kquency. The first cut showed higest CP value as cutting times increased the value tend to be lowered. Crude protein content in 3 and 4 cutting regimes were from 16.3 to 16.6% 17.4 to 17.9% respectively, but no significant effect were found among the main and sub-plot. ADF and NDF in 1995 were ranged from 38.2 to 39.9, 45.1 to 58.1% respectively. ADF was 2% more higher in 1996 compared to previous year and NDF was slightly higher in 1996. There was significant difference of ADF between main plot in 1995. .P, Ca, K and Mg of 200kg P level with 3 times cutting treatment in 1995 and 1996 were 0.32 and 0.23, 0.95 and 0.84, 3.24 and 2.87, and 0.36 and 0.26. Significant difference of K and Mg between cutting frequency was observed in 1995. Nitrogen fixing activity of 400kg P fertilizer plots were higher than 200kg P level and 3 times cutting plot also showed higher acetylene reduction activity than that of 4 cutting per year. Phosphate level and cutting frequency treatment of did not significantly affect nitrogen fixation activity.

• 한국약용작물학회지
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• 제24권6호
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• pp.444-450
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• 2016

Background: The roots of Atractylodes macrocephala Koidzumi contain atractylone, which is used to suppress appetite and indigestion caused by gastrointestinal disturbance. The present study was conducted to investigate the effect of several organic compost on the growth and root yield of A. macrocephala with organic fertilizer. Methods and Results: When organic fertilizer was applied basally, the average yield of 10 a was 184.6 kg in the HA (Hwanggeumjidae, organic material mix), 171.3 kg in the GG (Gyunbaeyangchegreen, bacterial cuture filtrate) and 175.0 kg in the CF (Customary fertilization, control) each other in practice of CF had no statistical significance. Atractylenolide I was significantly greater in the HA (0.036%) than the GG (0.034%) or CF (0.023%). With regard to the amount of organic fertilzer, 10 a yield was the most common of 203.0 kg at 2.0 times of the organic 1 (HA), conventional fertilization of 134.0 kg and 173.0 kg of no application was a statistically significant. Organic fertilizer 1 was 1.5 to 2.0 times, organic fertilizer was 2 to 1.5 times that were most suitable. Conclusions: The results of the present study indicated that HA and GG are the most suitable for the organic cultivation of A. macrocephala. The content of atractylone I was highest under the HA treatment and lowest under the CU (Chamjoa, oil cake), TG (Totogreen, plant oil cake) and HG (Heuksalgreen, Castor oil cake) treatment.

• 한국토양비료학회지
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• 제50권1호
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• pp.31-39
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• 2017

The effects of application of gypsum and fresh cattle manure on the yield of forage crop were investigated in Hwaong reclaimed land in Korea for 3 years from 2011 to 2013. This study was conducted to develop the practical application method of livestock manure as a fertilization source and a soil physico-chemical ameliorator for the cultivation of forage crop $Sorghum{\times}Sudangrass$ hybrid in newly reclaimed tidal land soil. Treatments with six applications were established with three replications; chemical fertilizer (CF), gypsum (G) $20Mg\;ha^{-1}$, G+fresh cattle manure (FCM) 100%, G+FCM 200%, G+FCM 300% and FCM 100% which referred to the application rate equivalent to the recommended amount of phosphate fertilization by soil test. The combined treatments of G+FCM increased soil organic matter, $Av.P_2O_5$ and exchangeable $Ca^{2+}$ contents while decreased exchangeable $Na^+$ and $Mg^{2+}$. The soil bulk density, soil hardness and soil aggregate formation were improved by G+FCM treatments. The dry matter yields of $Sorghum{\times}Sudangrass$ hybrid were significantly increased in proportion to the application rate of FCM. The phosphorus use efficiency showed the highest in the application level of G+FCM 100%, which seemed to be the results of reduced nutrient use efficiency by nutrient immobilization, leaching etc. when applied excessive amount of fresh animal manure.

• 한국유기농업학회지
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• 제12권4호
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• pp.411-425
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• 2004

The aim of this experiment was to investigate the effects of applying times and dilution rates of cattle slurry with water on dry matter yields and feed values of orchard grass. Cattle slurry was applied at the rates of average nitrogen fertilizer equivalent to 150kg/ha/year in 3 cutting frequency. Significantly higher dry matter yields than that of no fertilization (7.36 ton DM/ha) were recorded in the application of diluted cattle slurry (10.74~13.54 ton DM/ha) (p<0.05). Especially, this tendency was shown with higher annual dry matter yields at the partitioned dressing times, such as at the applications for 1st and 3rd growth, 2nd and 3rd growth, and 1st, 2nd and 3rd growth respectively. The yields of annual dry matter at fertilizing phosphorus, potassium and nitrogen were higher than no fertilization as 12.42 tons per ha. However, with application of diluted cattle slurry, dry matter yields per year (11.96 ton DM/ha) were significantly higher than those of fertilizing phosphorus and potassium (7.52 ton DM/ha) (p<0.05). The efficiencies of dry matter production with mineral and cattle slurry nitrogen application (kg DM/kg N) were 32.7 and 13.4~26.9kg DM/kg N respectively. Especially, these tendencies were higher in diluted application plots than in no-diluted application plots of cattle slurry. The contents of crude protein (CP, %), neutral detergent Eber (NDF, %), acid detergent fiber (ADF, %), annual yields of CP (kg/ha) and total digestible nutrient (TDNY, kg/ha) of orchardgrass were significantly higher at the application of diluted cattle Slurry than those at non-fertilizer application (p<0.05).

• 원예과학기술지
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• 제19권3호
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• pp.373-377
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• 2001

본 연구는 나팔나리(L. longiflorum) 'Gelria'(LG)와 자생 솔나리(L. cernuum, LC)와의 종간 교잡종을 육성할 목적으로 여러 가지 수분방법을 이용하여 수정 전 불화합성의 타파방법을 조사하였다. L. longiflorum 'Gelria'${\times}$L. cernuum의 교잡 수분 시 수분방법에 관계없이 모든 교배조합에서 착과가 이루어졌으며, 주두수분법에 비해 모든 화주절단수분법 처리구에서 착과율과 종자수가 높게 나타났다. 이들 중 화주를 2-3mm 남기고 자른 것과 화주를 모두 잘라버리고 자방기부에 수분시킨 처리구에서 각각 76.4%와 73.3%로 가장 높은 착과율을 나타내었다. 그러나 $LC{\times}LG$ 교잡에서는 전혀 착과되지 않았고 어떤 종자도 얻을 수 없었다. LG의 자가수분과 $LC{\times}LG$ 교잡에서는 속도에는 차이가 있었으나 수분 후 약 90시간 정도가 되면 자방까지 화분관이 신장하였다. 그러나$LC{\times}LG$ 교잡에서는 수분 후 90시간까지 자방에 도달하지 못하였고, 화주 중간에 선단이 비대되고 callose가 이상 집적된 기형적인 화분관이 많이 관찰되었다. 개화 후 수분시기도 착과율과 종자 형성률에 영향을 주었으며, 개화 후 3일에 수분하였을 때 가장 높은 종자수를 획득하였다. 주두 분비물질은 자방비대에는 영향을 미쳤으나, 수정배주수는 증가하지 않았다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.225-225
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• 2017

This study is to investigate the effect of split application and adapted fertilization application cultivation in stable medicinal herbs production of Acorus graminens S. And it accomplished one's purpose to GAP guide manual established standard method of cultivation technology, contribute to the enlargement of farm's income. Method of fertilization application were conducted under five condition compose to standard application(N-P-K-Compost: fermented mixed oil cake fertilizer applied at $4-2-6-300kg.10a^{-1}$, twofold application(N-P-K-Compost applied at $8-4-12-600kg.10a^{-1}$) cultivation. Compost (fermented mixed oil cake fertilizer) and fused phosphate applied at 100% of basal fertilizer in April 14. The split application level of standard application and twofold application(N-K) applied at 100%, 50%, 40% of basal fertilizer in April 14, 50% and 40% of top dressing were three times application (N-K) in June 1, July 15, August 15, 25% and 20% respectively. Transplanting with Naju varieties in May 12 by growing pot seedling for 40 days in 2015. Planting density were spaced 30 cm apart in rows 30 cm apart with black vinyl mulching on open cultivation. Plot design randomized block 3 repetition. Therefore, growth characteristics by 50% and 40% of top dressing were three times application (N-K) compared to standard application and twofold application (N-K) applied at 100% basal fertilizer, aerial part as a result were so many such amount of growth as number of leaf per plant, etc., Length of diameter and main root of subterranean part growth increment rising highest. Dried roots yields treated standard fertilizer 100% application($250kg.10a^{-1}$) compared to 100% from twofold application (N-K) applied at 50% basal fertilizer, 50% and 40% of top dressing were three times application increased by 16%, 20%. respectively.