• 대한한방부인과학회지
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• 제27권2호
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• pp.103-110
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• 2014

Objectives: This study was performed to assess whether herbal medicine and acupuncture is effective on clinical pregnancy. Methods: From february 2013 to july 2013, a prospective analysis study was performed in 6 patients after taking her medicine and acupuncture treatment. Results: After treatment, one patients naturally became pregnance and one patients became pregnance after in vitro fertilization-embryo transfer (IVF-ET). Conclusions: This study suggests that herbal medicine and acupuncture treatment shows possibility to increasing pregnancy rates.

• 한국수정란이식학회지
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• 제7권1호
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• pp.49-55
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• 1992

Animal experiments need numerous kinds of animal which are suitable for every research. About 300 mouse strains are developed up to the present, but they do not give satisfaction to every researchers. So we must build up the methods of breading animals which are newly developed and of maintenance of characteristics which were developed before. To maintain experimental animal is not only proceeding the generation but also increasing the animal populations, it needs geneticai control. Genetic factors which influence to reproduction are very important to maintain colony. These factors include lethal gene, chromosomal abberation, sterility gene, etc.. With the recent development of transgenic technology, scientists now can deliberately creat numerous specific animal models. To know how to manage the colony which has genetic defect on reproduction and transgenic mice is one of the key to study in vitro fertilization.

• 의료법학
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• 제10권1호
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• pp.77-116
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• 2009

Owing to the development of Biotechnology, the involvement of humans in life before birth has been increasing. This means the need for the protection of unborn life takes on new importance. The respect for life and human beings which is based on fundamental constitutional principle should still be respected under civil law. This study examines how methods of respect for life are embodied in civil liability law. In particular, it enunciates the protection of unborn life within time-flow. Lastly, it studies the instruments of the civil liability law and the extent of protection for a fetus from the process of fertilization of an ovum by a sperm, development into an embryo and implantation. Especially, it looks into when and how the subject of the right changes. Besides, it critically scrutinizes the opinions of leading case lawyers and the Constitutional Court which conclude that, in order for a fetus to become the subject of Damage law, it is required to be born alive to comply with precedent. Furthermore, it suggests an alternative interpretation theory.

• 대한한방부인과학회지
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• 제27권3호
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• pp.143-150
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• 2014

Objectives: The purpose of this study is to report a effect of herbal medicine at threatened abortion in the early stages of pregnancy. Methods: A 37-year-old female patient who had the abdomen pain with vaginal bleeding was taken Gyoaesamultang-gami and Antae-eum for 11 days at Dae-gu Haany University Hospital in Pohang. Results: During this treatment period, vaginal bleeding and abdomen pain were relieved. Also after all treatment, the effect had been continuing. Conclusions: Gyoaesamultang-gami is effective on threatened abortion. And the female patient deliver normally.

• Clinical and Experimental Reproductive Medicine
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• 제49권3호
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• pp.168-174
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• 2022

Refractory thin endometrium and recurrent implantation failure are among the most challenging infertility-related factors hindering successful pregnancy. Several adjuvant therapies have been investigated to increase endometrial thickness and the pregnancy rate, but the treatment effect is still minimal, and for many patients, these treatment methods can be quite costly and difficult to approach. Platelet-rich plasma (PRP) is an autologous concentration of platelets in plasma and has recently been elucidated as a better treatment option for these patients. PRP is rich in cytokines and growth factors, which are suggested to exert a regenerative effect at the level of the injured tissue. Another advantage of PRP is that it is easily obtained from the patient's own blood. We aimed to review the recent findings of PRP therapy used for patients with refractory thin endometrium and recurrent implantation failure.

• 대한간호학회지
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• 제50권5호
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• pp.671-685
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• 2020

Purpose: The purpose of this study was to develop a Coping Scale for Infertility-Women (CSI-W). Methods: The initial items were based on an extensive literature review and in-depth interviews with seven infertile women. Forty-three items were derived from a pilot survey. Data were collected from 216 women who had experienced intrauterine insemination (IUI) and in-vitro fertilization (IVF) more than once. The data were analyzed to verify the reliability and validity of the scale. Results: Seven factors containing 28 items (four factors containing 17 items for active coping and three factors containing 11 items for passive coping) were extracted from the exploratory factor analysis to verify the construct validity. The four factors of active coping were confrontation, self-control, seeking social support (spouse), and seeking social support (colleagues and experts). The three factors of passive coping were distancing, escape, and avoidance. These items were verified through convergent, discriminant, and concurrent validity testing. The internal consistency reliability was acceptable (active coping: Cronbach's α = .78; passive coping: Cronbach's α = .81). Conclusion: As its validity and reliability have been verified through various methods, the CSI-W can contribute to assessing the coping strategies of infertile women.

• 한국연초학회지
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• 제25권1호
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• pp.27-33
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• 2003

This study was conducted to determine the changes of nitrogen compounds in lamina and midrib during the curing process with the different curing methods, such as priming and stalk-cut curing. After KB 108 burley tobacco was cultivated by the different fetilization levels such as standard and 20% higher, only the tips and leaf were harvested. Though the contents of total alkaloid and total nitrogen were similar in lamina, midrib showed a very low contents of those components by the different curing method and fertilization levels. The content of nitrate-nitrogen in lamina increased during a middle of curing process, and then these compound was decreased during an end of curing process by stalk-cut curing method. Nitrate-nitrogen contents in the lamina by the priming curing showed a high level caused by rapid drying process during an end of curing process. That component in midrib was 5-6 times higher than that of lamina. The contents of ammonia-nitrogen in the lamina and midrib were increased during a curing process. Though those amount in tips showed a similar by a different fetilization and curing method, midrib of stalk-cut curing showed a slow increasing during a curing process.

• Journal of Animal Science and Technology
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• 제44권6호
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• pp.701-710
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• 2002

본 연구는 소 난자의 초자화 동결 방법을 설정하기 위하여 체외성숙 소 난자에서 난구세포가 부착된 상태 또는 제거한 상태로 microdrop (MD) 방법과 straw (Straw) 방법을 이용하여 초자화 동결하여 생존율을 검사하였다. 동결 융해난자는 a) 단위발생을 유도하였고 b) 체외수정 후 전핵 형성을 관찰하였으며 c)체외수정 후 수정란 발달을 검사하였다. 초자화 동결 난자의 생존율은 MD 방법을 이용하였을 때가 Straw를 이용하였을 때 보다 높았다 (92.50 vs. 74.19%, p<0.05). MD 방법을 이용하였을 때 대부분의 난자가 생존을 하였다. 단위발생을 유도하였을 때 난할율과 배반포 발달율은 MD (45.05%, 10.81%, p<0.05)가 Straw 방법 보다 높았다 (27.17%, 6.52%, p<0.05). 난구세포의 부착 유무에 따라 동결 융해 후 체외수정 하여 자웅 전핵 형성을 각각 검사하였다. 난구세포 제거 난자에서는 MD와 Straw 방법으로 동결 융해하였을 때 차이가 없었다(80.36% vs. 67.31 %, p<0.05). 정상 수정율 (2PN)에서는 세 처리간에 차이가 없었다 (Fresh; 54.55% vs. MD; 42.22% vs. Straw; 37.14%, p>0.05). 그러나 미수정란 (<1PN)은 Fresh 난자가 동결융해 난자보다 유의적으로 낮았다 (Fresh; 32.47% vs. MD; 57.78% and Straw 62.86%, p<0.05). 다정자 침입은 (3PN) 신선란 (12,99%)에서 발생하였으나 동결 융해 난자에서는 발생하지 않았다. 난구세포가 제거된 난자에서, 정상 수정율 (2PN)은 Fresh와 동결 융해 난자간에 유의적인 차이를 보였다 (Fresh; 59.38% vs. MD; 17.31% and Straw; 30.43%, p<0.05). 또한 미수정율 (<1PN)에 있어서도 신선란과 동결 융해난은 유의적인 차이를 보였다 (Fresh; 23.44% vs. MD; 73.08% and Straw 58.70%, p<0.05). 다정자 침입 (3PN, >4PN)은 신선란과 동결 융해란 모두에서 나타났다. 체외수정 후, 난구세포가 부착된 난자의 2세포기 발달율은 신선란에 비하여 MD 또는 Straw 처리구에서 유의적으로 낮았다 (Fresh; 81.76% vs. MD; 22.22% and Straw; 11.36%, p<0.05). 동결융해난자는 배반포 발달율에 있어서도 신선란에 비하여 유의적으로 낮았다 (Fresh; 28.38 vs. MD; 1.71% and Straw 0%, p<0.05). 난구세포가 제거된 난자에서 2세포기 발달율은 신선란과 MD에서 차이가 없었다 (27.59% vs. 19.25%, p<0.05), 그러나 배반포 발달율에 있어서는 신선란이 MD 또는 Straw 처리구보다 유의적으로 높았다 (4.31% vs. 0.62% and 0%, respectively; p<0.05). 이상의 결과에서, 초자화 동결융해한 소 난자는 체외수정 후 배반포로 발달이 가능함을 나타내주고 있다.

• 한국발생생물학회:학술대회논문집
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• 한국발생생물학회 2003년도 제3회 국제심포지움 및 학술대회
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• pp.108-108
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• 2003

The purpose of this is to investigate the effects of vitrification in open pulled straws (OPS) on in vitro survival of porcine embryos. Blastocysts were produced by in vitro fertilization of slaughterhouse-derived, in vitro matured oocytes with frozen-thawed boar semen, and subsequent culture on granulosa cell monolayer. After frozen-thawing, embryos were culture in NCSU-23 medium with 5 mM hypotaurine, 4 mg/$m\ell$ BSA and 10 ng/$m\ell$ for 48 hrs to survival tests. When blastocysts were frozen-thawed by OPS methods, the embryos with normal morphology were 32.1, 34.5 and 38.9 % in early blastocyst, blastocyst and expanded blastocyat stages. The rates of partial damaged embryos were significantly (P<0.05) higher in early biastocysts than expanded blastocysts. In another experiment, the embryos frozen by OPS methods were cultured for 48 hrs for survival and developmental rates in vitro. The proportions of embryos hatched were 11.8, 20.2 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. On the other hand, The proportions of embryo with normal morphology after culture were 23.5, 25.0 and 33.3% in embryos frozen-thawed at stages of early blastocyst, blastocyst and expanded embryos. These finding indicate the possible broader application for OPS methods that this procedure described is relatively harmless, that it can be used for blastocysts of different developmental stages.

• Clinical and Experimental Reproductive Medicine
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• 제20권2호
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• pp.107-115
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• 1993

Procedures to separate motile. normal & motile and acrosome-reacted sperm with high efficiency have clinical application in Assisted Reproductive Technology in terms of increasing the probability of fertilization by a normal sperm and subsequent normal embryonic development. This study evaluated the effects of 10 sperm preparation techniques [Swim-up from a washed pellet (SU). Swim-up from semen (SO). Continuous Percoll Gradients I (PIC). Discontinuous Percoll Gradients I (PID). Continuous Percoll Gradients II(P II C). Discontinuous Percoll Gradients II(P II D), SpermPrep (SFC). Wang's tube (WT). Albumin Gradients (AG), Low temperature capacitation (LTC)] on motility (%), normal morphology (%), motile sperm recovery rate(%). morphologically normal & motile sperm recovery rate (%), true acrosome reaction (%) and fertilizing ability. A P II D proved to be an effective means of separating morphologically normal & motile sperm. Our results indicated the P II D has advantages as compared with other methods in terms of recovery rate. enhancement of motility and normal morphology. And a LTC seems to be an effective means of enhancing the true acrosome reaction and fertilizing ability. These results suggest that the combined method of LTC and P II D for separation of morphologically normal & motile sperm and acrosome reacted sperm may be a useful procedure for intrauterine insemination and in vitro fertilization in the management of male factor infertility as well as for isolation of subpopulation of sperm for basic research.