• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 춘계학술대회
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• pp.97-97
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• 2019

Chaga mushroom and Cheonggukjang have been used in alternative medicine. In this study, we determined the anti-obesity effects of fermented Chaga-Cheonggukjang (FCC), an extract prepared by secondary fermentation of a mixture of Cheonggukjang and Chaga by Lactobacillus acidophilus on highfat diet (HFD)-induced mice. Male ICR mice were fed a normal diet or HFD in the presence or absence of 3% and 5% FCC (FCC3 and FCC5). After 3 months, the mice were sacrificed, and serum and tissue samples were examined. Body weight and epididymal fat pad (EFP) weight were significantly lowered in FCC3 and FCC5 groups compared to those in the HFD control group. FCC supplementation suppressed serum triglyceride (TG) and increased serum HDL levels. Serum GOT, GPT, leptin levels and hepatic COX-2 mRNA expression were significantly higher in the HFD groups, and these increases were significantly attenuated by FCC supplementation. FCC suppressed body weight and EFP weight gain, as well as inflammatory responses in the liver in HFD-fed mice. Thus, FCC supplementation may have protective effects for obesity-related disease.

• 한국균학회지
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• 제47권2호
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• pp.153-163
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• 2019

본 연구는 갯버들(Salix gracilistyla)의 화장품 소재로의 활용가능성을 판단하기 위해, 기존 일반적인 방법이 아닌 균사배양 방법을 통해 가능성을 확인하였고, 구체적으로는 대표적인 약용버섯인 불로초(Ganoderma lucidum) 균사의 배양을 통하여 그 효능을 확인하였다. 총 폴리페놀 및 플라보노이드 함량과 DPPH 라디칼소거능 분석결과에서는, 갯버들 추출물(SGE)이 불로초 균사배양 갯버들 추출물(GLSGE) 보다 높게 평가되었으나, 콜라게나제 활성 억제 평가에서는 불로초 균사배양 갯버들 추출물(GLSGE)이 갯버들 추출물(SGE)과 비교하여 상대적으로 낮게 평가되었다. 그럼에도 불구하고, 불로초 균사배양 갯버들 추출물(GLSGE)은 갯버들 추출물(SGE)을 처리한 경우보다, HDFn 세포의 생존율을 높은 수준으로 증가시켰으며, HDFn 세포의 제1형 프로콜라겐의 생합성 또한 매우 높은 수준으로 증가시키는 것이 확인되었다. 결과적으로 앞선 실험을 통해, 불로초 균사배양 갯버들 추출물이 항노화 활성을 가지는 기능성 화장품 소재로 활용 가능성이 있다고 사료되며, 기능성 소재의 효과를 더욱 증진시키기 위한 방안으로 불로초 균사의 배양이 효과적이라는 것을 의미한다.

• 한국응용과학기술학회지
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• 제40권6호
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• pp.1557-1566
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• 2023

곽향은 서양에서 Korea mint라고 불릴 정도로 허브와 쓰임새가 매우 유사하다. 서양의 허브는 화장품의 소재로 많이 사용하지만 곽향은 우리나라에서 약재로 더 많이 사용되어 화장품 소재로서의 기능성을 비교해보았다. 곽향 추출물과 추출물을 이용한 유산균과 효모 발효물, 에센셜오일, 플로럴워터에 대한 항산화, 미백, 폴리페놀, 플라보노이드, 항균력 실험을 진행하였다. 항산화 활성은 모든 샘플에서 높은 효과가 보였고, 미백과 폴리페놀, 항균력에서 에센셜오일이 가장 높은 효과가 보였다. 반면에 플라보노이드는 70% 에탄올 추출물에서 가장 높은 효과가 보였다. 이런 결과로 에센셜오일이 기능적으로 가장 큰 효과가 있었다. 하지만 추출물을 가지고 효모 발효를 진행한 시료들이 에센셜오일 만큼의 효과가 있다는 것을 확인해 에센셜오일의 적은 추출량에 비해 효모 발효를 진행한 시료가 화장품소재로서 충분한 가치가 있다고 본다.

• 한국약용작물학회지
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• 제13권6호
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• pp.293-299
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• 2005

식물자원을 이용하여 식물추출물 발효식품을 제조하는데 있어서 적합한 발효기간을 설정하기 위하여 발효기간의 경과에 따른 발효액의 품질특성과 효소활성 및 항산화활성 등의 변화를 조사하였다. 발효기간이 경과함에 따라 pH와 $^{\circ}BX$는 점차 낮아졌으며, 점도는 3개월 경과 시 현저히 낮아졌다. 색도는 발효기간이 증가함에 따라 무를 제외한 시료에서 L값과 a값은 증가되고, 은 감소되었으며, 무는 L, a, 값이 모두 감소하였다. 발효액 중의 amylase활성은 전체 시료에서 활성을 나타내었고 특히 브로컬리, 가지, 양배추. 치커리, 두릅, 무에서 발효 전에 비하여 발효 6개월 경과 후에 각각 460, 430, 190, 420, 560, 260 units로 활성이 크게 증가하였다. invertase는 무와 토마토에서 활성을 나타내었으며 발효 전에 각각 170, 130 units 에서 6개월 경과 후 200과 460 unit로 증가되었다. cellulase는 두릅, 무, 토마토에서 활성을 나타냈으며 발효 전에 55, 10, 95 units 에서 발효 6개월 경과 후에 자각 100, 280, 140units 증가하였다. 총 페놀 화합물 함량은 발효가 진행됨에 따라 증가하여 $2{\sim}4$개월 발효 시 가장 높았고, 항산화활성도는 발효 4개월 경과 시에 가장 높았으며 그 이후에는 감소하였다. Tyrosinase와 SOD 유사활성은 전체적으로 약한 활성을 나타내었다. 발효액의 관능적인 특성을 조사하였을 때 두릅의 경우에는 발효 기간의 경과에 따라서 두릅 고유의 냄새, 단 냄새, 새콤한 냄새는 감소하였으며 두릅맛, 쓴맛, 떫은맛은 감소하여 전체적인 기호도가 3개월 발효액에서 가장 높았다. 무의 경우에는 발효기간이 증가함에 따라 무 냄새와 매운맛이 감소하고, 단맛이 증가하였으며, 전체적인 기호도가 발효 3개월에서 가장 좋은 것으로 평가되었다. 이상의 결과를 종합해 볼 때 식물자원 추출물의 발효기간은 $3{\sim}4$개월 정도가 적합할 것으로 판단된다.

• 한국약용작물학회지
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• 제20권5호
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• pp.339-344
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• 2012

This study was carried out to research microorganisms having the antifungal activity against ginseng Alternaria blight pathogen Alternaria panax and ginseng anthracnose pathogen Colletotrichum gloeosporioides. Eleven Bacillus strains. were isolated from Korean traditional soybean paste and Kimchi. Among the 11 isolates, DJ5, KC1, KC2 and KC4 showing antagonistic activity on the mycelial growth of A. panax and C. gloeosporioides in pairing culture were finally selected as the antagonistic microorganisms. Based on 16s rRNA sequence and phylogenetic tree analysis, they were identified as Bacillus spp.. The selected microorganisms were investigated antagonistic activity by measured leaf-segment colonization in pot test. When Bacillus sp. were injected after A. panax treatment, KC1, KC2 and KC4 showed similar effect to chemical pesticides treated control. To measure preventive effect of Bacillus sp, antagonistic microorganisms were injected and C. gloeosporioides was treated in pot. When measuring the effectiveness for the prevention of Anthracnose, All Bacillus spp. showed approximately 83~90 % degree of superior preventive effect. In general, The four Bacillus spp. isolated from Korean traditional fermented foods showed therapeutic effect of Alternaria blight and preventive effect of Anthracnose.

• 한국약용작물학회지
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• 제9권4호
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• pp.290-294
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• 2001

삼백초주 제조중 기능성 성분의 이행성 정도를 측정하고자, 증자백미를 기준으로 개량누룩 5%, 양조용수 120%, 삼백초 건엽 분말 2% 또는 건근분말 5%를 첨가하여 삼백초주를 제조하였고, 발효기간에 따른 페놀성 화합물의 함량 변화를 조사하여 얻은 결과를 요약하면 다음과 같다. 1. 삼백초주의 total phenolics, flavonoid phenolics 및 quercetin 관련물질의 함량은 발효기간이 경과할수록 많았다. 2. 삼백초주의 total phenolics와 flavonoid phenolics 함량은 잎삼백초주에서 각각 ${762.5{\sim}1,054.7mg/l}$와 ${387.8{\sim}537.2mg/l}$, 뿌리삼백초주는 각각 ${608.4{\sim}928.8\;mg/l}$와 ${329.5{\sim}481.7\;mg/l}$로 잎삼백초주에서 높았다. 3. Quecetin 함량은 잎삼백초주에서는 ${3.5{\sim}3.8mg/l}$로 발효기간 간에 차이가 미미하였으며, 뿌리 삼백초주에서는 검출되지 않았다. Quercetin 관련 물질의 총함량은 잎삼백초주에서 ${72.3{\sim}84.2\;mg/l}$, 뿌리삼백초주 ${21.6{\sim}32.2\;mg/l}$로 잎삼백초주에서 높았다. 4. 뿌리삼백초주에 비하여 잎삼백초주에서 total phenolics, flavonoid phenolics 및 quercetin 관련물질의 함량이 현저히 높아 잎을 이용하여 삼백초주를 제조하는 것이 효과적이었다.

• 한국약용작물학회지
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• 제18권6호
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• pp.429-438
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• 2010

The use of cellulolytic lactic acid bacteria in new method to prepare high nutrition complementary foods was investigated. For the screening of cellulolytic lactic acid bacteria, more than 1,150 bacterial colony were isolated from diluted infant feces samples. A typical strain which appeared the most excellent cellulolytic activities was identified novel acidophilic Enterococcus sp. TO-94 through the results of morphological, biochemical and chemotaxonomic characteristics and 16S rDNA sequencing. The optimal lactic acid fermentation conditions of Omija(Schizandra chinensis Baillon) by Enterococcus sp. TO-94 were as follows: pH and temperature were 3.0 and $37^{\circ}C$, respectively, and fermentation time was 20hrs. The fructose and glucose were major free sugar and the contents were 5.83 and 4.30 mg/g after fermentation, respectively. The contents of lactic acid and acetic acid were 9.84 mg/g and 2.08 mg/g after fermentation, respectively. The vitamin $B_1$, $B_2$, niacin, folic acid and C were major vitamin in the fermented broth, the contents were 1.5~3 times higher than those of initial fermentation time. Also, the contents of polyphenol and anthocyanine were 3.8 and 1.2 times higher than those of initial fermentation time.

• 한국약용작물학회지
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• 제23권1호
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• pp.37-42
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• 2015

This study was to investigate anti-skin wrinkle effect of Agastache rugosa Kentz extracts by extraction processes. In the comparison of the effect of the solvent extraction, the extracts by 70% ethanol (EE) showed better biological activities that those by hot water. Therefore, further fermented Agastache rugosa was applied to 70% ethanol extraction process (FEE). FEE showed higher DPPH scavenging activity of 62.98% than EE's 62.71% at $1.0mg/m{\ell}$, but there was no significant. Elastase inhibition was measured 23.0% from FEE at $1.0mg/m{\ell}$. Cytotoxicity showed the highest 16.26% from FEE, this value is safe in the cell experiment. Collagen production showed $113.1ng/m{\ell}$ from FEE, on the other hand EE was measured $77.4ng/m{\ell}$ in adding $1.0mg/m{\ell}$. MMP-1 production was observed $1398pg/m{\ell}$ from FEE and EE was measured $1632pg/m{\ell}$. These results were found the highest antioxidant and anti-wrinkle effect. As a result, it was also confirmed that anti-skin wrinkle activities of the Agastache rugosa Kentz extract was correlated with anti-oxidant activities.

• 산업식품공학
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• 제13권2호
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• pp.130-137
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• 2009

This study evaluated the potential of utilizing the osmotic solution from dried mango processing as alternative raw material for mango wine making. Fermentation was carried out using two kinds of yeast strains Saccharomyces bayanus, Lalvin EC-1118 and Saccharomyces cerevisiae, Lalvin D-47 at 20$^{\circ}C$ for 28 days. Physicochemical analysis during fermentation was performed for each treatment and the resulting wine samples were analyzed for color, volatiles and sensory properties. Results of physicochemical analysis between the two fermenting samples as well as the wine samples show almost similar results regardless of the yeast strains. Wine color of sample wines after storage were not significantly different at p<0.05 and when compared with a commercial mango wine. From the volatile analysis, esters and alcohols constituted majority of the compounds. Production of several esters, alcohols, acids and terpenes were affected by yeast strain used in fermentation. Results of sensory analysis showed that wines fermented by S. bayanus EC-1118 strain was more acceptable although sensory scores between the treatments and the reference wine showed significant differences in all the attributes evaluated, except for bitterness. The utilization of osmotic solution from dried mango process could produce similar properties with existing commercial mango wines although there is still need for further work on the improvement of some sensory attributes of the mango wines.

• Journal of Microbiology and Biotechnology
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• 제31권6호
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• pp.855-866
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• 2021

The effects of various carbon sources on mycelial growth and polysaccharide synthesis of the medicinal fungus Inonotus obliquus in liquid fermentation were investigated. After 12-d fermentation, mycelial biomass, polysaccharide yield, and polysaccharide content were significantly higher in Glc+Lac group (glucose and lactose used as combined carbon source) than in other groups. Crude polysaccharides (CIOPs) and the derivative neutral polysaccharides (NIOPs) were obtained from mycelia fermented using Glc, fructose (Fru), Lac, or Glc+Lac as carbon source. Molecular weights of four NIOPs (termed as NIOPG, NIOPF, NIOPL, and NIOPGL) were respectively 780.90, 1105.00, 25.32, and 10.28 kDa. Monosaccharide composition analyses revealed that NIOPs were composed of Glc, Man, and Gal at different molar ratios. The NIOPs were classified as α-type heteropolysaccharides with 1→2, 1→3, 1→4, 1→6 linkages in differing proportions. In in vitro cell proliferation assays, viability of RAW264.7 macrophages was more strongly enhanced by NIOPL or NIOPGL than by NIOPG or NIOPF, and proliferation of HeLa or S180 tumor cells was more strongly inhibited by NIOPG or NIOPGL than by NIOPF or NIOPL, indicating that immune-enhancing and anti-tumor activities of NIOPs were substantially affected by carbon source. qRT-PCR analysis revealed that expression levels of phosphoglucose isomerase (PGI) and UDP-Glc 4-epimerase (UGE), two key genes involved in polysaccharide synthesis, varied depending on carbon source. Our findings, taken together, clearly demonstrate that carbon source plays an essential role in determining structure and activities of I. obliquus polysaccharides by regulating expression of key genes in polysaccharide biosynthetic pathway.