• Journal of Microbiology and Biotechnology
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• v.32 no.7
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• pp.902-910
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• 2022

The ginsenoside compound K (C-K) is widely used in traditional medicines, nutritional supplements, and cosmetics owing to its diverse pharmacological activities. Although many studies on C-K production have been conducted, fermentation is reported to produce C-K with low concentration and productivity. In the present study, addition of an inducer and optimization of the carbon and nitrogen sources in the medium were performed using response surface methodology to increase the C-K production via fermentation by Aspergillus tubingensis, a generally recognized as safe fungus. The optimized inducer and carbon and nitrogen sources were 2 g/l rice straw, 10 g/l sucrose, and 10 g/l soy protein concentrate, respectively, and they resulted in a 3.1-fold increase in the concentration and productivity of C-K (0.22 g/l and 1.52 mg/l/h, respectively) compared to those used before optimization without inducer (0.071 g/l and 0.49 mg/l/h, respectively). The feeding methods of American ginseng extract (AGE), including feeding timing, feeding concentration, and feeding frequency, were also optimized. Under the optimized conditions, A. tubingensis produced 3.96 mM (2.47 g/l) C-K at 144 h by feeding two times with 8 g/l AGE at 48 and 60 h, with a productivity of 17.1 mg/l/h. The concentration and productivity of C-K after optimization of feeding methods were 11-fold higher than those before the optimization (0.22 g/l and 1.52 mg/l/h, respectively). Thus, the optimization for the feeding methods of ginseng extract is an efficient strategy to increase C-K production. To our knowledge, this is the highest reported C-K concentration and productivity via fermentation reported so far.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.33 no.6
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• pp.607-613
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• 2009

In this paper, we made a simple paper feeding system which is one of MTS (media transport system) and controllers. The plant has a flexible paper and two driving rollers and two driven rollers. The control system has two conventional PID controllers. Skew angle and feeding speed of MTS deteriorate the quality of feeding system. In order to control a feeding speed and skew of feeding paper, we control rotational velocity of two driving rollers. Therefore, this controller has two inputs and two outputs as MIMO (multi-input and multi-output) system. The control inputs were the feeding speed and the skew displacement of the paper. The control outputs were the rotational velocity to each driving roller. To find appropriate PID gains of two controllers, we proposed an optimization technique. We assume the system variables and performance of a whole system as follows. PID gains of two controllers for skew and feeding speed are system variables. System performance is both skew and feeding speed. We simulates to making mathematical correlation using global Kriging interpolation. To find appropriate value of system variables, optimization method is simulation in sequence as following method. First, the optimization solver simulates with DOE (design of experiment) tables to find correlation equation of both system variable and performances. Then, the solver guesses the appropriate values and simulates if the system variables are appropriate or not. If the result of validation doesn't satisfy the convergence and iteration tolerance, the solver makes a new Kriging models and iterates this sequence until satisfy the tolerances.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.107-108
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• 2006

This paper shows the design optimization of the paper feeding mechanism under dynamic behavior by using commercial codes of RecurDyn/MTT2D and RecurDyn/AutoDesign which are developed by functionBay, Inc. A virtual mockup for dynamics analysis of the paper feeding mechanism is build on RecurDyn/MTT2D and is simulated. Flexible paper is represented as a series of rigid bars connected by revolute joints and rotational spring dampers. Paper is fed by a contact and friction mechanism on rollers or guides. The slip of the paper and nip force of rollers are measured to estimate the system performance. After a simulation, these performances are automatically send to RecurDyn/AutoDesign which is a sequential approximate optimization tool based on the response surface modeling. RecurDyn/AutoDesign makes the approximate objective function and computes the optimized design points of the design variables and gives them to analysis tool. And then the simulation is repeated with the updated design variables. These processes are repeated until finding a tolerable design optimization. In this paper, a paper feeding mechanism is introduced and it is optimized with the proposed algorithms.

• Proceedings of the KSME Conference
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• 2001.11a
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• pp.848-852
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• 2001

A new approach to the design of bank note counters is suggested. Modern bank note counters are equipped with several types of sensors for counting and detection of counterfeit bank notes. To achieve higher speed of counting while maintaining the sensing capability, longitudinal feeding scheme is proposed. Several merits of longitudinal feeding are discussed. Taguchi method is used for the optimization of the feeding mechanism.

• Journal of Institute of Control, Robotics and Systems
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• v.5 no.7
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• pp.876-882
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• 1999

It is difficult to determine the feeding rate of coagulant in the water treatment process, due to nonlinearity, multivariables and slow response characteristics, etc. To deal with this difficulty, the genetic-fuzzy system was used in determining the feeding rate of the coagulant. The genetic algorithms are excellently robust in complex optimization problems. Since it uses randomized operators and searches for the best chromosome without auxiliary informations from a population consists of codings of parameter set. To apply this algorithms, we made the lookup table and membership function from the actual operation data of the water treatment process. We determined optimum dosages of coagulant(LAS) by the fuzzy operation, and compared it with the feeding rate of the actual operation data.

• Biotechnology and Bioprocess Engineering:BBE
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• v.5 no.4
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• pp.275-287
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• 2000

Fermentation strategies for recombinant protein production in Pichia pastoris have been investigated and are reviewed here. Characteristics of the expression system, such as phenotypes and carbon utilization, are summarized. Recently reported results such as growth model establishment, app58lication of a methanol sensor, optimization of substrate feeding strategy, DOstat controller design, mixed feed technology, and perfusion and continuous culture are discussed in detail.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.81-84
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• 2001

A real time optimization algorithm for fed-batch cultures of recombinant yeast to determine the optimal substrate feed rate profile has been developed. Its development involved four key steps: (1) development of reliable adaptive model. (2) development of optimization algorithm. (3) design of on-line model update algorithm to be incorporated into the optimization algorithm and (4) experimental validation. A recombinant Saccharomyces cerevisiae producing human parathyroid hormone (hPTH) was chosen as the model strain. It was found to be very successful in maintaining cell growth and galactose consumption at leigh levels, thus resulting in significant improvements in the productivity (up to 2.1 times) and intact hPTH concentration (up to 1.5 times) compared with the case of an intermittent glucose and galactose, or galactose feeding.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2007.05a
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• pp.314-317
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• 2007

Automotive rear subframe of aluminum tube was developed by using hydroforming process, based on the numerical analysis and physical tryouts. In the previous study, the effect of prebending was evaluated on the basis of forming limit diagram which had been obtained from free bulging, T-shape forming and cross-shape forming, using the developed tube hydroformability testing system. In order to get the sound products, appropriate internal pressure is to be imposed corresponding to the axial feeding. In this study, the loading path, the combination of internal pressure and axial feeding during the process, was optimized to ensure minimum thickness variation and dimensional accuracy, by using response surface method.

• Journal of Korean Society on Water Environment
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• v.23 no.3
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• pp.367-370
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• 2007

Numerous sets of simulation were conducted in order to find out the optimum operational conditions of the existing BNR process using GPS-X program. The model of ASM3 and modified Bio-P module were applied for simulations. From the result of this study, effluent quality was closely related with the step feeding rate and influent C/N ratio. The effluent TN concentration seemed to be significantly affected by step feeding rate at the low C/N condition. But at the high C/N condition, the effluent concentration of TP rather than that of TN was affected by the control of step feeding rate.

• Transactions of Materials Processing
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• v.13 no.2
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• pp.142-147
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• 2004

Three-dimensional rigid-plastic finite element analysis has been performed to optimize open die forging process to make round bar. In the round bar forging, it is difficult to optimize process parameters in the operational environments. Therefore in this study, finite element method is used to analyze the practice of open die forging, focusing on the effects of reduction, feeding pitch and rotation angle for optimal forging pass designs. The soundness of forging process has been estimated by the smoothness and roundness of the bar at various combination of feeding pitches and rotation angles. From the test result, process conditions to make round bar having precise dimensional accuracy have been proposed.