• Title/Summary/Keyword: feed analysis methods

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Design of A Narrow-Band Orthomode Transducer Operating at Ku-Band (Ku-대역 협대역 직교모드 변환기 설계)

  • Li Jun-Wen;Kim Jin-Young;Ko Han-Woong;Park Dong-Hee;Ahn Bierng-Chearl
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.15 no.12 s.91
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    • pp.1123-1130
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    • 2004
  • In this paper, a technique is presented f3r the design of a structurally simple narrow-band orthomode transducer (OMT) operating at 12/14 GHz frequency bands. The proposed OMT has such a structure that a horizontally polarized wave incident on the circular waveguide of the common port is directed to the rectangular waveguide of the straight port, while the vertically polarized wave is directed to the rectangular waveguide of the side port. Firstly methods are developed f3r designing individual parts of the OMT based on the waveguide theory and the theory of impedance matching. Secondly based on developed methods the whole OMT structure is designed using a commercial electromagnetic field analysis software. The validity of the proposed design technique is confirmed by fabricating and testing the designed OMT.

Effects of short-term fasting on in vivo rumen microbiota and in vitro rumen fermentation characteristics

  • Kim, Jong Nam;Song, Jaeyong;Kim, Eun Joong;Chang, Jongsoo;Kim, Chang-Hyun;Seo, Seongwon;Chang, Moon Baek;Bae, Gui-Seck
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.6
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    • pp.776-782
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    • 2019
  • Objective: Fasting may lead to changes in the microbiota and activity in the rumen. In the present study, the effects of fasting on rumen microbiota and the impact of fasting on in vitro rumen fermentation were evaluated using molecular culture-independent methods. Methods: Three ruminally cannulated Holstein steers were fed rice straw and concentrates. The ruminal fluids were obtained from the same steers 2 h after the morning feeding (control) and 24 h after fasting (fasting). The ruminal fluid was filtrated through four layers of muslin, collected for a culture-independent microbial analysis, and used to determine the in vitro rumen fermentation characteristics. Total DNA was extracted from both control and fasting ruminal fluids. The rumen microbiota was assessed using denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction. Microbial activity was evaluated in control and fasting steers at various intervals using in vitro batch culture with rice straw and concentrate at a ratio of 60:40. Results: Fasting for 24 h slightly affected the microbiota structure in the rumen as determined by DGGE. Additionally, several microorganisms, including Anaerovibrio lipolytica, Eubacterium ruminantium, Prevotella albensis, Prevotella ruminicola, and Ruminobacter amylophilus, decreased in number after fasting. In addition, using the ruminal fluid as the inoculum after 24 h of fasting, the fermentation characteristics differed from those obtained using non-fasted ruminal fluid. Compared with the control, the fasting showed higher total gas production, ammonia, and microbial protein production (p<0.05). No significant differences, however, was observed in pH and dry matter digestibility. Conclusion: When in vitro techniques are used to evaluate feed, the use of the ruminal fluid from fasted animals should be used with caution.

Study on the reduction of heterocyclic amines by marinated natural materials in pork belly

  • Hea Jin, Kang;Seung Yun, Lee;Da Young, Lee;Ji Hyeop, Kang;Jae Hyeon, Kim;Hyun Woo, Kim;Jae Won, Jeong;Dong Hoon, Oh;Sun Jin, Hur
    • Journal of Animal Science and Technology
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    • v.64 no.6
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    • pp.1245-1258
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    • 2022
  • This study was conducted to determine the effect of natural ingredient seasoning on the reduction of heterocyclic amine (HCA) production that may occur when pork belly is cooked at a very high temperature for a long time. Pork belly seasoned with natural ingredients, such as natural spices, blackcurrant, and gochujang, was cooked using the most common cooking methods, such as boiling, pan fry, and barbecue. HCAs in pork belly were extracted through solid-phase extraction and analyzed via high-performance liquid chromatography. For short-term toxicity, a mouse model was used to analyze weight, feed intake, organ weight, and length; hematology and serology analysis were also performed. Results revealed that HCAs formed only when heating was performed at a very high temperature for a long time, not under general cooking conditions. Although the toxicity levels were not dangerous, the method showing the relatively highest toxicity among various cooking methods was barbecue, and the natural material with the highest toxicity reduction effect was blackcurrant. Furthermore, seasoning pork belly with natural materials containing a large amount of antioxidants, such as vitamin C, can reduce the production of toxic substances, such as HCAs, even if pork belly is heated to high temperatures.

Nutritional evaluation of new alternative types of dog foods including raw and cooked homemade-style diets

  • Boyeon Choi;San Kim;Goo Jang
    • Journal of Veterinary Science
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    • v.24 no.5
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    • pp.63.1-63.9
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    • 2023
  • Background: New alternative types of pet foods such as raw and cooked homemadestyle diets containing human food ingredients have been introduced due to a trend of pet humanization and diversification of consumer needs. Objectives: To evaluate nutritional adequacy of new alternative types of dog foods containing human food ingredients as maintenance diets for dogs. Methods: Eleven homemade-style foods for adult dogs were purchased from online channel in Korea and analyzed to evaluate nutritional adequacy for adult dogs. Nutrients analyzed included crude protein, amino acids, crude fat, fatty acids, and minerals. Results: Crude protein and amino acids in all products satisfied Association of American Feed Control Officials (AAFCO) requirements. Crude fat in one of 11 products did not meet AAFCO requirements. The most deficient minerals were selenium (10 of 11, 90.9%), copper (five of 11, 45.5%), zinc (five of 11, 45.5%), potassium (three of 11, 27.3%), calcium (three of 11, 27.3%), iron (two of 11, 18.2%), and magnesium (one of 11, 9.1%). Six products were not in the range of the recommended Ca:P ratio in AAFCO dog food maintenance nutrient profiles. Conclusions: This study performed nutritional evaluation of raw and cooked homemadestyle foods as maintenance diets for adult dogs. Some nutritional inadequacies were observed including some minerals, Ca:P ratio, and omega-6:omega-3 fatty acid ratio, although three products (26.2%) satisfied the AAFCO standard except selenium. Overall, the data suggest a need for accurate nutritional adequacy statement for consumers based on proper methods to validate the formula.

Analysis of Suffocating Accidents in Confined Spaces in the Past 10 Years (2005-2015) (밀폐공간 질식재해 자료 분석을 통한 질식재해 요인 분석 (2005-2015))

  • Lee, Jung Wan;Kim, Tae Hyeung;Ha, Hyun Chul;Piao, Cheng Xu;Ahn, Kwangseog
    • Journal of Korean Society of Occupational and Environmental Hygiene
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    • v.26 no.4
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    • pp.436-444
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    • 2016
  • Objectives: Despite recent efforts to prevent suffocation disasters, a number of confined space accidents still happen and each year deaths continue to occur. There have been insufficient studies on the dangers of various potential disaster sites, such as manholes, septic tanks, reactors, and other tanks according to type, characteristics, task-specific disasters, equipment specific disasters, etc. The purpose of this study was to analyze recent suffocation disasters based on place and properties. Methods: In this study, we analyzed confined space accident cases from 2005 to 2015 in Korea and grouped them by type, size, monthly occurrence, continuous service period, accident location, person-specific group, age, employment, structural work and subcontracting work. We studied examples of accidents developed in other countries. Results: (1) We reviewed confined-space accident statistics, compared legal standards and analyzed cases of suffocation accidents in the United States and Japan. (2) Using a case study report from the Korea Occupational Safety & Health Agency, we specified confined-space accident statistics based on place, person-specific group, age, employment, structural work and subcontracting work. As a result we generated some precautions about confined-space accidents for the prevention of such accidents. Conclusions: Conclusions: Statistical analysis of recent suffocation disaster cases was performed to establish improvement measures, compare practices from developed countries, and develop precautionary measures accordingly. In this study, we presented the causes of disaster that occur in a confined space and proposed related preventive measures.

Changes in microbial population and chemical composition of corn stover during field exposure and effects on silage fermentation and in vitro digestibility

  • Sun, Lin;Wang, Zhijun;Gentu, Ge;Jia, Yushan;Hou, Meiling;Cai, Yimin
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.6
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    • pp.815-825
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    • 2019
  • Objective: To effectively use corn stover resources as animal feed, the changes in microbial population and chemical composition of corn stover during field exposure, and their silage fermentation and in vitro digestibility were studied. Methods: Corn cultivars (Jintian, Jinnuo, and Xianyu) stovers from 4 random sections of the field were harvested at the preliminary dough stage of maturity on September 2, 2015. The corn stover exposed in the field for 0, 7, 15, 30, 60, 90, and 180 d, and their silages at 60 d of ensiling were used for the analysis of microbial population, chemical composition, fermentation quality, and in vitro digestibility. Data were analyzed with a completely randomized $3{\times}6$ [corn stover cultivar $(C){\times}exposure$ d (D)] factorial treatment design. Analysis of variance was performed using SAS ver. 9.0 software (SAS Institute Inc., Cary, NC, USA). Results: Aerobic bacteria were dominant population in fresh corn stover. After ensiling, the lactic acid bacteria (LAB) became the dominant bacteria, while other microbes decreased or dropped below the detection level. The crude protein (CP) and water-soluble carbohydrate (WSC) for fresh stover were 6.74% to 9.51% and 11.75% to 13.21% on a dry matter basis, respectively. After exposure, the CP and WSC contents decreased greatly. Fresh stover had a relatively low dry matter while high WSC content and LAB counts, producing silage of good quality, but the dry stover did not. Silage fermentation inhibited nutrient loss and improved the fermentation quality and in vitro digestibility. Conclusion: The results confirm that fresh corn stover has good ensiling characteristics and that it can produce silage of good quality.

Extraction of dietary fibers from cassava pulp and cassava distiller's dried grains and assessment of their components using Fourier transform infrared spectroscopy to determine their further use as a functional feed in animal diets

  • Okrathok, Supattra;Thumanu, Kanjana;Pukkung, Chayanan;Molee, Wittawat;Khempaka, Sutisa
    • Animal Bioscience
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    • v.35 no.7
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    • pp.1048-1058
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    • 2022
  • Objective: The present study was to investigate the extraction conditions of dietary fiber from dried cassava pulp (DCP) and cassava distiller's dried grains (CDG) under different NaOH concentrations, and the Fourier transform infrared (FTIR) was used to determine the dietary fiber components. Methods: The dried samples (DCP and CDG) were treated with various concentrations of NaOH at levels of 2%, 4%, 6%, and 8% using a completely randomized design with 4 replications of each. After extraction, the residual DCP and CDG dietary fiber were dried in a hot air oven at 55℃ to 60℃. Finally, the oven dried extracted dietary fiber was powdered to a particle size of 1 mm. Both extracted dietary fibers were analyzed for their chemical composition and determined by FTIR. Results: The DCP and CDG treated with NaOH linearly or quadratically or cubically (p<0.05) increased the total dietary fiber (TDF) and insoluble fiber (IDF). The optimal conditions for extracting dietary fiber from DCP and CDG were under treatment with 6% and 4% NaOH, respectively, as these conditions yielded the highest TDF and IDF contents. These results were associated with the FTIR spectra integration for a semi-quantitative analysis, which obtained the highest cellulose content in dietary fiber extracted from DCP and CDG with 6% and 4% NaOH solution, respectively. The principal component analysis illustrated clear separation of spectral distribution in cassava pulp extracted dietary fiber (DFCP) and cassava distiller's dried grains extracted dietary fiber (DFCDG) when treated with 6% and 4% NaOH, respectively. Conclusion: The optimal conditions for the extraction of dietary fiber from DCP and CDG were treatment with 6% and 4% NaOH solution, respectively. In addition, FTIR spectroscopy proved itself to be a powerful tool for fiber identification.

Underlying mechanisms of phosphodiesterase 10A and glutamate-ammonia ligase genes that regulate inosine monophosphate deposition and thereby affect muscle tenderness in Jingyuan chickens

  • Wang, Weizhen;Zhang, Juan;Hu, Honghong;Yu, Baojun;He, Jintong;Yao, Tingting;Gu, Yaling;Cai, Zhengyun;Xin, Guosheng
    • Animal Bioscience
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    • v.35 no.11
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    • pp.1771-1786
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    • 2022
  • Objective: Inosine monophosphate (IMP) is a key factor that imparts of meat flavor. Differences in the IMP content in the muscles were evaluated to improve chicken meat quality. Methods: For this study, the IMP content was detected by high performance liquid chromatography. The gene expression profiles of Jingyuan chickens with different feeding patterns and different sexes were analyzed by RNA-sequencing (RNA-seq). Results: Breast muscle IMP content in free-range chickens was extremely significantly higher than that of caged chickens (p<0.01). Breast muscle IMP content in hens was also higher than that of cocks, but the difference was not significant. Correlation analysis showed that the breast muscle IMP content in caged hens and cocks was negatively correlated with the shear force, and the breast muscle IMP content in free-range hens was significantly negatively correlated with the shear force (p<0.05). The two key genes associated with IMP synthesis in chickens with different feeding patterns were glutamate-ammonia ligase (GLUL) and phosphodiesterase 10A (PDE10A). Bioinformatics analysis revealed that the GLUL and PDE10A genes are involved in glutamine biosynthesis and purine salvage pathways respectively. In addition, GLUL expression was positively correlated with the IMP content in caged and free-range chickens, and PDE10A expression was significantly positively correlated with the IMP content in caged and free-range chickens (p<0.05). Conclusion: These findings will facilitate the comprehension of the deposition of IMP in the muscles and thereby aid the process of selection and breeding of good quality local chickens.

Detection and environmental unintentional release monitoring of living modified maize (Zea mays L.) in Gyeonggi-do of South Korea in 2014 (2014년 경기지역 유전자변형 옥수수 모니터링 및 발견현황)

  • Shin, Su Young;Moon, Jeong Chan;Choi, Wonkyun;Kim, Il Ryong;Jo, Beom-Ho;Lee, Jung Ro
    • Journal of Plant Biotechnology
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    • v.45 no.1
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    • pp.77-82
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    • 2018
  • In South Korea, LM crops are not allowed to grow locally, but have been allowed to be imported as food and feed purposes. Currently, the typical LMO imports are continuously increasing in the region of South Korea. In 2014, we carried out a review of the environmental release monitoring of LM maize (Zea mays L.) in Gyeonggi-do of South Korea, and analyzed volunteer samples using strip test kits and polymerase chain reaction (PCR) methods. We thereby collected 44 volunteers of released LM maize in 169 locations around ports, from roadsides, feed factories and stockbreeding farmhouses. We found 4 positive samples at 3 sites using strip test kits. Based on the PCR analysis, the LM maize plants were found using event-specific primers. These results suggested that our monitoring is necessary to detect the presence of released LM maize in the natural environment of South Korea.

Analysis of Broken Rice Separation Efficiency of a Laboratory Indented Cylinder Separator

  • Kim, Myoung Ho;Park, Seung Je
    • Journal of Biosystems Engineering
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    • v.38 no.2
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    • pp.95-102
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    • 2013
  • Purpose: Using a laboratory indented cylinder separator, broken rice separation experiments were conducted and the characteristics of the separation process were studied to provide information for developing a prototype indented cylinder broken rice separator. Methods: Rice (Ilmi variety) milled in a local RPC was used for the experiment. Rice kernels were classified into four groups according to their length l; whole kernels (I > 3.75 mm), semi-whole kernels (2.5 < I < 3.75 mm), broken kernels (1.75 < I < 2.5 mm), and foreign matters (I < 1.75 mm). A laboratory grain cleaner, Labofix '90 (Schmidt AG, Germany) was used for the experiments. Experiments were designed as a $4{\times}4$ factorial arrangement in randomized blocks with three replications. Cylinder rotational speeds (17, 34, 51, 68 rpm) and trough angles (15, 37.5, 60, $82.5^{\circ}$) were the two factors and feed rates (25, 50 kg/h), indent shapes (Us, $S_1$ type), and indent sizes (2.5, 3.75 mm) were treated as the blocks. Two 125 g samples and one 125 g sample were taken at the cylinder outlet and from the trough, respectively. The whole, semi-whole, and broken kernel weight ratio of the samples and feed was determined by a rice sizing device. From these weight ratios, purities, degrees of extraction and coefficient of separation efficiency were calculated. Results: Trough angle, cylinder speed, and their interaction on the coefficient of separation efficiency were statistically significant. Cylinder speed of 17, 34, and 51 rpm made the most effective separation when the trough angle was $15^{\circ}$ or $37.5^{\circ}$, $60^{\circ}$, and $82.5^{\circ}$, respectively. Maximum values of coefficient of separation efficiency were in the range of 60 to 70% except when the indent size was 2.5 mm and were recorded for the combinations of low cylinder speed (17 rpm) with medium trough angle ($37.5^{\circ}$ or $60^{\circ}$). Indent shape did not appear to make any noticeable difference in separation efficiency. Conclusions: Due to the interaction effect, the trough angle needs to be increased appropriately when an increase in cylinder speed is made if a rapid drop of effectiveness of separation should be avoided. In commercial applications, $S_1$ type indents are preferred because of their better manufacturability and easier maintenance. For successful separation of broken kernels, the indent size should be set slightly bigger than the actual sizes of broken kernels: an indent size of 3.0 mm for separating broken kernels shorter than 2.5 mm.