• Title/Summary/Keyword: fed batch

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Optimization of SOD Biosynthesis by Controlling Sucrose Concentration in the Culture of Carrot Hairy Root

  • Kim, Ji-Hyeon;Yoo, Young-Je
    • Journal of Microbiology and Biotechnology
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    • v.12 no.4
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    • pp.617-621
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    • 2002
  • In order to optimize the carrot hairy root culture for SOD production, a fed-batch culture of hairy roots was performed in a bioreactor. Maximum SOD activity was obtained when the hairy roots were transferred to the MS medium containing 110 g/1 concentration of sucrose. By controlling the sucrose concentration (70 g/1 sucrose for growth and 110 g/1 sucrose far production, respectively) In a two-stage fed-batch culture, 29 g/1 of the hairy roots was obtained based on the final dry mass. The volumetrically determined SOD activity and productivity in the fed-batch culture were about 6 times higher than those from the flask culture containing sucrose at 30 g/1 concentration.

Production of an Anticoagulant Hirudin by Fed-batch and Continuous Cell Recycle Fermentations Using Recombinant Saccharomyces cerevisiae (유가식과 세포재순환 연속공정을 이용한 항혈전제 hirudin의 생산)

  • 최치민;김명동;이상기;서진호
    • KSBB Journal
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    • v.13 no.4
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    • pp.456-460
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    • 1998
  • Fed-batch fermentations were carried out in order to improve the efficiency of hirudin production by recombinant Saccharomyces cerevisiae. A fed-batch fermentation done with the optimized semi-synthetic medium resulted in a maximum hirudin concentration of 342mg/$\ell$ by keeping a galactose concentrations between 10 and 30g/$\ell$ which corresponded to a 11.4-fold increase in hirudin concentration compared with the simple bach fermentation done with the same medium. Comparison of the chromatographic pattern of proteins in the growth medium clearly showed that the use of the semi-synthetic medium is more advantageous for separation of hirudin than the case o fusing the complex medium. Continuous cell recycle fermentation done at dilution rate of 0.1h-1 and an inlet galactose concentration of 100g/$\ell$ yielded a maximum hirudin productivity of 19.1mg hirudin/$\ell$$.$h.

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Glucoamylase Production in Batch and Fed-Batch Solid State Fermentation: Effect of Maltose or Starch Addition

  • Bertolin, Telma Elita;Jorge Alberto Vieira Costa;Gean Delise Leal Pasquali
    • Journal of Microbiology and Biotechnology
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    • v.11 no.1
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    • pp.13-16
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    • 2001
  • Maltose and soluble starch were used as secondary sources of carbon for glucoamylase production by Aspergillus awamori in solid state fermentation. During batch cultivation, maltose above 2.5%(w/w) repressed glucoamylase production, but, by adding either 2.5% (w/w) maltose or 1.25% (w/w) soluble starch to fed-batch cultivations, glucoamylase activity was increased by 15% and 170% over standard medium, respectively. The data showed that maltose is a weak inducer of glucoamylase production in solid stat fermentation.

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Optimization of Producing Liquid Fuel from Photosynthetic Algal Growth

  • Pak, Jin-Hong;Lee, Shin-Young
    • Journal of Microbiology and Biotechnology
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    • v.1 no.2
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    • pp.111-115
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    • 1991
  • The green alga, Dunaliella salina under fed-batch cultivation produced 51.12 mg of hydrocarbon per liter with maintaining 0.313 (g dry wt/l). About 20% of hydrocarbon production yield based on dry biomass was obtained from both batch and fed-batch processes. Optimum culture conditions of light intensity, pH and salt concentration were obtained as 0.0080 (kJ/$cm^2$/h), 8.0 and 1.4 (g of NaCl/l), respectively by response surface analysis. The production of hydrocarbons in D. salina was closely correlated to cell growth. Fed-batch cultivation produced more hydrocarbons and maintained better cell growth than a batch process.

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High Concentrated Spore Production of Bacillus thuringliensis by Fed-Batch Processes (유가식 배양공정에 의한 Bacillus thurngiensis의 고농도 포자생산)

  • 박창열;유연우
    • KSBB Journal
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    • v.15 no.3
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    • pp.219-225
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    • 2000
  • Both the production of high spore concentration and high insecticidal activity are required in the production of Bacillus thuringiensis to be used for the bacterial insecticide. In the production of high cell and spore concentrations of B. thuringiensis the continuous fed-batch culture(CFBC) and intermittent fed-batch culture(IFBC) were investigated at $28^{\circ}C$ by maintaining 40% dissolved oxygen concentration. When the final glucose concentration was 50 g/L the maximum viable cell number obtained using the CFBC with linear gradient feeding was $9.37{\times}109$ cells/mL and maximum spore concentration was $8.33{\times}109$ spores/mL which was approximately 84.4% yield of spore formation. When the final glucose concentration was 100 g/L the aximum viable cell and spore concentrations obtained using the IFBC with pH-statb were $1.38{\times}$1010 cells/mL and $1.35{\times}1010$ spores/mL respectively and the yield of spore formation was approximately 97.8%.

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Controlled Fed-Batch Cultivation of Escherichia coli Mutant for L-Tryptophan Production (대장균 변이주의 조절식 유가배양법에 의한 L-트립토판 생산)

  • Lee, In-Young;Kim, Myung-Kuk;Kho, Yung-Hee;Kwak, Moo-Young;Lee, Hosull;Lee, Sun-Bok
    • Microbiology and Biotechnology Letters
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    • v.16 no.6
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    • pp.450-456
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    • 1988
  • For optimal production of L-tryptophan using a regulatory mutant of Escherichia coli the relationship between product formation and acid production was investigated. Experimental results showed that the production level of L-tryptophan was lowered as the specific acid production rate increased. In order to reduce the amount of acid produced during the fermentation, a controlled fed-batch fermentation was employed. In this fed-batch process, the feed rate of the nutrient feed medium was controlled in relation to the oxygen level in the culture and thus the growth of the cells was regulated in such n way that the oxygen demand of the culture could not exceed the oxygen sup-ply. When E. coli cells were cultivated in a controlled fed-batch mode of tormentor operation, the specific acid production rate was significantly reduced and L-tryptophan production was increased as much as five times that obtained in a conventional fed-batch fermentation.

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Production of Emulsan by Acinetobacter calcoaceticus RAG-1 under Various Culture Modes (여러 배양방법하에서 Acinetobacter calcoaceticus RAG-1에 의한 Emulsan의 생산)

  • 강병철;이필경장호남
    • KSBB Journal
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    • v.6 no.4
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    • pp.389-394
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    • 1991
  • Emulsan is an extracellular emulsifying agent produced by the hydrocarbon-degrading Acinetobacter species RAG-1. In this study emulsan production of Acinetobacter calcpaceticus RAG-1 was investigated under various culture modes such as batch, fed-batch, membrane cell recycle, and continuous culture. The productions of emulsan under both ethanol-sufficient fed-batch and membrane cell recycle cultures were all 15.0U/ml, which was 53% increase in emulsan activity compared to that of pH controlled batch culture. Emulsan production was found to be strongly dependent on the residual ethanol concentration. In continuous culture the emulsan productivity increased with dilution rate.

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The Effect of Glutamine on Production of Tissue-type Plasminogen Activator from Recombinant Human Melanoma Cells in Glutamine-limited Fed-batch Cultivation

  • Kim, Hyun-Goo;Kim, Tae-Ho;Kim, Dae-Seok;Park, Kyung-Yu;Park, jin-seo;Ahn, Chol;Lee, Jin-Ha;Lee, Hyeon-Yong
    • Journal of Microbiology and Biotechnology
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    • v.6 no.4
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    • pp.295-298
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    • 1996
  • Under glutamine-limited condition, $2\times10^6$ (viable cells/ml) of maximum cell density and 13.5 ($\mu g$/ml) of tissue-type Plasminogen Activators (tPA) production were maintained by spike feeding fresh medium in fed-batch cultivation of human recombinant melanoma cells. It showed that tPA production was much seriously affected than cell growth according to initial glutamine concentrations. Above 3.4 (mmol/I) of glutamine concentration both cell growth and tPA production were not much affected by increasing initial glutamine concentration. Glutamine depleted situation was occurred at latter periods of batch and fed-batch cultivations below 5.4 (mmole/I) of initial glutamine concentration. It also showed that maximum glutamine consumption and ammonia evolution rates were closely related to initial glutamine concentrations. Maximum specific tPA production rate was estimated as $8.1\times19^{-6}$ ($\mu g$/cells/h) at 3.4(mmol/I) of glutamine concentration, which is higher than that from other batch and fed-batch processes.

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Growth of Issatchenkia orientalis in Aerobic Batch and Fed-batch Cultures

  • Shin, Hyung-Tai;Lim, Yoo-Beom;Koh, Jong-Ho;Kim, Jong-Yun;Baig, Soon-Yong;Lee, Jae-Heung
    • Journal of Microbiology
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    • v.40 no.1
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    • pp.82-85
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    • 2002
  • The aerobic batch growth of Issatchenkia orientalis DY252 with glucose and fructose medium was investigated at 32$\^{C}$ and pH 5.0. Aerobic ethanol production was evident with yeast I, orientalis. A diauxic lag of about 1 h between growth on glucose and growth on ethanol during batch culture was observed. However, no diauxic growth occurred with fructose. As the incubation temperature was increased from 32 to 39$\^{C}$, viability at the end of each batch culture declined significantly, from 93 to 43%, Unlike the effect of temperature, viability was not greatly affected by incubation pH, and cell yield values in a range of 0.45-0.48 were obtained. In order to overcome overflow metabolism, a fedbatch culture under glucose limitation was carried out. Compared with aerobic batch culture, about 10% improvement in cell yield was achieved with a fed-batch culture in optimal conditions.

Fed-batch Culture of Enterococcus faecalis RKY1 for L[+]-Lactic Acid Production (L[+]-Lactic Acid 생산을 위한 Enterococcus faecalis RKY1의 유가식 배양)

  • Wee Young Jung;Kim Jin Nam;Yun Jong Sun;Park Don Hee;Kim Do Man;Ryu Hwa Won
    • KSBB Journal
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    • v.19 no.5
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    • pp.410-414
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    • 2004
  • Fed-batch cultures of Enterococcus faecalis RKY1 were performed to maximize the L(+)-Iactic acid concentration in the bioreactor. The highest lactic acid concentration was obtained at around 225 g/L by intermittent feeding the concentrated glucose media containing 500 g/L of glucose and 15 g/L (or 75 g/L) of yeast extract. However, in all fed-batch cultures, volumetric productivities of lactic acid gradually decreased due to the inhibitory effect of lactic acid produced during the fermentation. The highest value of lactic acid concentration obtained in this work corresponded to around 1.5-fold increase compared with conventional batch fermentation.