• 한국식품과학회지
• /
• 제27권2호
• /
• pp.185-192
• /
• 1995

함평군 손불면의 특산물인 엽삭젓을 염농도 18%와 22%로 각각 엽삭젓을 제조하여 발효가 진행되는 동안 발효와 관련된 미생물과 여러가지 화학성분의 변화를 측정한 결과 발효 초기에 지질과 단백질의 감소와 총균수의 증가가 있었고 총산도의 증가는 어느정도 pH와 반비례적인 경향을 보였고, 아미노태질소와 지질의 산가는 급증한 후 큰 변화를 보이지 않았다. 선도의 척도라 할 수 있는 휘발성염기질소는 뼈국물을 넣어줌으로서 그 증가가 억제됨을 보이지만 전체적으로 볼때 발효 기간중 꾸준히 증가하였으며 염도 22% 엽삭젓보다는 염도 18% 엽삭젓이 훨씬 높은 수치를 보였다. 지방산 조성은 palmitic acid($C_{16:0}$)가 20% 정도 함유되어 가장 많았고, palmitoleic acid($C_{16:1}$), eicosapentaenoic acid($C_{20:5}$), oleic acid($C_{18:1}$), myristic acid($C_{14:0}$)순으로 함유되어 이들이 전체 지방산의 77.3%를 차지하였으며 EPA와 DHA도 약 20%정도 함유되어 있었으며 발효 기간중 큰 변화가 없었으며 염도에 따른 차이도 없었다. 총 유리아미노산도 휘발성 염기질소와 마찬가지로 계속 증가하지만 뼈국물을 넣고 젓국물 달여 붓기를 하므로서 상당히 그 증가의 지연이 이루어짐을 알 수 있었으며 유리아미노산 조성은 원료육의 경우 aspartic acid, threonine, histidine이 주류를 이루었으나 숙성된 염도 18% 엽삭젓의 경우는 leucine, tyrosine, glutamic acid, valine, isoleucine이, 염도 22% 엽삭젓의 경우는 alanine, glutamic acid, leucine, methionine이 주류를 이루었다. 이상의 결과를 비교해 볼때 엽삭젓에는 고도불포화지방산 특히 EPA가 많이 함유되어 있으며 염도 18%와 22%의 엽삭젓의 염도간에 큰 차이는 없었으나 VBN등의 변화에 있어서 엽삭젓 제조의 염도로는 18%에 비하여 22%가 더 적합한 것으로 생각되었다.

• Journal of Nutrition and Health
• /
• 제26권9호
• /
• pp.1049-1070
• /
• 1993

The purpose of this study is to investigate dietary factors which influence the conditions of Korean fatty liver patients. The subjects were 83 fatty liver patients living in city, ages of 10 to 60 yrs old, and they were the same patients studied 9 months ago in previous study. The patients were groupsed into 3 groups(improved, similar, worsened groups) according to the changes in disease condition. Nutrient and alcohol intakes and health status were investigated. In improved group, the patients tended to lose weight, the number of exercising patients was increased, the consumptions of calorie, carbohydrate, calcium, phosphorus were decreased. And alcohol intake was decreased markedly and serum total amino acids(AA), EAA, NEAA, BCAA and AMAA contents, EAA/NEAA and BCAA/AMAA ratios were increased. In worsened group, patients tended to gain weight, the number of exercising patients decreased, the consumptions of calorie, protein, carbohydrate, calcium, phosphorus and niacin decreased but vitamin. A intake increased. And also, in this group, alcohol intake of male patients decreased and that of female slightly increased, and serum EAA, BCAA and AMAA contents, EAA/NEAA and BCAA/AMAA ratios, and total fatty acid contents increased and total AA and NEAA contents decreased. In conclusion, it is desirable to reduced weight, stress, alcohol, salt and animal fat consumptions, and to exercise, and to take adequate amount and quality of protein to improve conditions of fatty liver patients.

• 한국식품영양과학회지
• /
• 제14권1호
• /
• pp.67-71
• /
• 1985

삶은 콩에 Aspergillus oryzae를 접종하여 3일간 제국(製麴)한 다음 식염을 첨가하여 45일간 숙성(熟成)시켰을때, 된장 숙성과정(熟成過程)중 된장 함유지질(含有脂質)의 변화에 관한 일련의 실험을 행하여 다음과 같은 결과를 얻었다. 숙성(熟成)중 수분(水分)은 54% 내외, 식염함량(食鹽含量)은 12% 내외 (건물(乾物)기준), 총질소(總窒素)는 5.6% 내외 (아미노태 질소 $1,532{\sim}2,011mg%$), pH 는 5.82내외, 온도 $27^{\circ}C$ 내외를 보였고 추출지질 (chloroform-methanol extractables 22%내외)의 요오드가(價)(숙성최종일(熟成最終日) : 98.2)는 저하되는 경향이었으며 산가(酸價)는 숙성중 현저하게 증가 (숙성최종일(熟成最終日) : 27.1) 하였다. 된장숙성중 총지질(總脂質)중의 중성지질(中性脂質)함량은 계속 증가하였으나(숙성최종일(熟成最終日) : 94.3%), 인(燐) 및 당지질(糖脂質)의 함량은 미소(微少)하지만 상대적으로 감소하였다. 그리고 숙성이 진행됨에 따라 비극성지질(非極性脂質) 획분에서 free fatty acid 및 esterified sterol 등이 증가하였으나, 극성지질(極性脂質) 획분에서의 각 성분은 현저한 변화는 없었다. 한편, 숙성초일(初日) 및 최종일(最終日)의 총지질(總脂質), triglyceride 및 free fatty acid 획분들의 지방산조성(脂肪酸組成)을 살펴본 결과, linoleic, oleic, palmitic acids 들이 주요성분이고 숙성기간중 이들 조성비(組成比)의 경미(輕微)한 변화가 있었다.

• Bulletin of the Korean Chemical Society
• /
• 제35권12호
• /
• pp.3509-3513
• /
• 2014

Phospholipase D (PLD) catalyzed the generation of phosphatidic acid (PA) from phosphatidylcholine (PC) at the outer layer of the vesicles prepared through layer by layer via a double emulsion technique. The generation induced a curvature change in the vesicles, which eventually led them to fuse each other. The ratio of two-fatty-acid-tail ethanolamine (PE) to one-fatty-acid-tail ethanolamine (PE) was found to acquire the condition where the mixed-phospholipid vesicles were stable identically with pure two-fatty-acid-tail PC. The effect of the outer-layer mixture on the PLD-induced vesicle fusion was investigated using the fluorescence intensity change. 8-Aminonaph-thalene-1,3,6-trisulfonic acid disodium salt (ANTS) and p-Xylene-bis(N-pyridinium bromide) (DPX) were encapsulated in the vesicles, respectively, for the quantification of the fusion. The fluorescence scale was calibrated with the fluorescence of a 1/1 mixture of ANTS and DPX vesicles in NaCl buffer taken as 100% fluorescence (0% fusion) and the vesicles containing both ANTS and DPX as 0% fluorescence (100% fusion), considering the leakage into the medium studied directly in a separate experiment using vesicles containing both ANTS and DPX. The fusion data for each composition were acquired with the subtraction of the leakage from the quenching. From the monitoring, the vesicle fusion caused by the PLD reaction seems dominantly to occur rather than the vesicle lysis, because the composition effect on the fusion was observed identically with that on the change in the vesicle structure. Furthermore, the diameter measurements also support the fusion dominancy.

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권9호
• /
• pp.1296-1302
• /
• 2008

This work was conducted to study the performance, carcass characteristics, chemical composition and fatty acid profile of the Longissimus muscle (LM) of first-generation Purunã (n = 7, PUR1), second-generation Purunã (n = 9, PUR2), and 1/2 Puruna vs. 1/2 Canchin (n = 13, PUCA) bulls finished in a feedlot. The animals averaged 18 months of age at the beginning of the experiment. The animals were kept in a feedlot from 18 to 22 months of age. They were fed twice a day with corn silage ad libitum, along with soybean meal, cracked corn, urea, limestone and mineral salt. PUR1 bulls had greater (p<0.05) final weight (496.0 kg) than PUCA bulls (449.2 kg). However, there was no difference (p>0.05) between PUR1 and PUR2 (472.0 kg), and between PUR2 and PUCA. Hot carcass weight was similar (p>0.05) among the different genetic groups. Hot carcass dressing percentage was similar (p>0.10) between PUR1 (50.4%) and PUCA (53.8%), and higher (p<0.10) than PUR2 (48.6%). Cushion thickness was greater (p<0.05) in PUCA bulls (27.12 cm). The other parameters were similar (p<0.05) among the three genetic groups. Total lipid and cholesterol contents were higher (p<0.01) in PUR2. Moisture, ash and crude protein contents were similar (p>0.05) among the three genetic groups. Saturated fatty acid (SFA) levels were higher (p<0.10) in PUR1 and PUR2 (51.58% and 50.41%, respectively). Polyunsaturated fatty acid levels were higher (p<0.05) in PUR1 and PUCA (6.50% and 8.29%, respectively). N-6, n-3 and PUFA/SFA ratios were higher (p<0.05) in PUCA. MUFA and n-6/n-3 ratios were similar (p>0.05) among the different genetic groups.

• 한국응용과학기술학회지
• /
• 제10권1호
• /
• pp.83-91
• /
• 1993

The syntheses of urocanic acid esters was optimized, starting from p-toluenesulfonic acid salt of this acid and long chain fatty alcohols in the organic solvent and extracting water from it by means of azeotropic Compound. The salts of these urocanic acid esters showed amphoteric properties, but their micellization enhances their rate of hydrolysis leading to the free amine. Nevertheless the long chain gives to the esters themselves an amphoteric character allowing their solubilization in micellar media and in microemulsions the result, yield could enhanced.

• 한국가정과학회지
• /
• 제3권2호
• /
• pp.77-89
• /
• 2000

In this study, to activate the industrialization and to improve the quality of Toha-jeot by shortening the fermentation period, we investigated the changes in the nutritional components of Toha-jeot. salt-fermented Toha shrimp( Caridina denticulata denticulata $D_{E}$ $H_{AAN}$) which was salted with a low-salt group and high-salt group during fermentation. In this experiment. there are four groups of Toha-jeot which were manufactured with 15% ratio of common salt: the first group containing 2% wheat bran (w2%-L). the second high-salt group containing 2% wheat bran( w2%-H) , the third low-salt group containing 4% wheat bran (w4%-L) and the last high-salt group containing 4% wheat bran(w4%-H). These four groups were refrigerated at 4${\pm}$1$^{\circ}C$ and then taken out for analysis at three month intervals during 9 month. Among the free amino acid contents in Toha-jeot, 22 kinds were detected. 6 month after the fermentation when the quantity of the amino acid contents in Toha-jeot is highest, ornitine, glutamic acid, leucine. alanine. lysine and valine occupy the majority, in the order of abundance. In cases of nucleotides. 6 month after the fermentation. from the groups w2%-L, w2%-H and w4%-L, inosine and IMP were not detected. and hypoxanthine, AMP, ADP were detected but 9 month after the fermentation ADP was not detected. The main constituents of fatty acid were as follows : (a) from w2%-L, w2%-H, 6 month after the fermentation. $C16:0$, $C12:0$, $C18:1$, $C18:3$, and $C16:1$. (b) from w4%-L. 6 month after the fermentation, $C18:3$, $C16:0$, $C12:0$ and $C18:1$. (c) from W4%-H, $C16:0$, $C12:0$, $C18:3$ and $C18:1$. In case of mineral contents. Na, Ca. K. Mg, Fe. Zn, Mn and Cu were detected according to the magnitude of the quantity. From the group w4%-H, high quantity of Na was detected during the total fermentation period. In case of color value, from the groups w2%. the values of L. a. b were highest after 6 month fermentation and were decreased after 9 month fermentation, while from groups w4%, the values of L, a, b were gradually decreased after 3 month fermentation.ion.

• 한국수산과학회지
• /
• 제47권5호
• /
• pp.474-481
• /
• 2014

This study examined the effect of pretreated sandfish Arctoscopus japonicus meat as a surimi complex for preparing sandfish flavored fish paste. To prepare the sandfish-flavored paste, fine chopped sandfish meat including backbone was added in a ratio of 0 to 50 wt.% to thawed Alaska pollock Theragra chalcogramma surimi to make a mixed surimi gel. To prepare the sandfish-flavored paste, the mixed surimi was ground with salt using a silent cutter, mixed with starch and stabilizers 0.2% transglutaminase and gluconolactone 0.3%, stuffed in a rectangular container, left for 3 h at $25^{\circ}C$, cooked in hot water for 30 min at $90^{\circ}C$, and finally chilled for 20 min at $4^{\circ}C$. The effects of the pretreatment of sandfish meat were investigated by analyzing the quality of the paste produced. The proximate composition of FP (fish paste containing 40% steam-cooked sandfish meat and 0.3% gluconolactone) was moisture 76.1%, crude protein 12.0%, crude fat 3.8%, carbohydrate 6.1%, and ash 2.0%. The major minerals in FP were Na (23.77 mg/L), Mg (1.46 mg/L), Zn (1.04 mg/L), and Fe (0.41 mg/L), and the major free amino acids were taurine, anserine, alanine, and glutamic acid. The monounsaturated fatty acid content of FP was 566.22 mg%, and the polyunsaturated fatty acid content was 498.43 mg%. The n-3 fatty acid content was 398.01 mg%, and C20:5n-3 (218.85 mg %) was a major component.

• 공업화학
• /
• 제4권1호
• /
• pp.54-63
• /
• 1993

Dodecanoic acid와 tetraethylenepentamine을 반응시켜서 1, 2-disubstituted imidazoline을 얻었고, 이것을 양이온화시켜 유화형 1, 2-disubstituted imidazolinium salt를 합성하였다. 또한 docosanoic acid와 hydroxyethylethylenediamine을 반응시켜 중간생성물인 2-docosamido-2'-docosanoyloxydiethylamine을 얻었고, 다시 우레아와 반응시켜서 지방산 카르바미드를 얻었다. 이것을 역시 양이온화시켜 유화형 지방산카르바미드염을 합성하였다. 합성된 각종 염들을 블렌딩시켜 유연제를 제조한 후, 아크릴섬유에 유연처리를 하여 유연성, 평활성 및 대전방지성을 측정한 결과, 유연성과 평활성이 양호하였고 약간의 대전방지성도 보유하였다. 또한 강연도시험과 pH변화에 따른 촉감의 내세탁성 시험결과를 통하여 제조된 유연제가 내구성 유연제임을 입증하였으며, SEM으로 유연처리된 아크릴섬유의 표면구조를 관찰하였다.

• 한국식품영양과학회지
• /
• 제27권4호
• /
• pp.603-608
• /
• 1998

Lipid oxidation in ascidian was studied when fresh, deshelled and sliced meats were fermented for 50 days at 5$\pm$2$^{\circ}C$ with 8%(w/w) salt and 0.1% papain. Antioxidative effects of butylated hydroxytoluene(BHT) and carotenoid extracts from ascidian tunic on lipid oxidation and oxidationrelated discoloration of ascidian meat during fermentation were investigated. Changes in peroxide value, carbonyl value, thiobarbituric acid value, fatty acids composition, the loss of total carotenoid and sensory evaluation were determined to assess the rancidity. Peroxide and carbonyl values in BHT and carotenoid extract treatments increased less than those of the control during fermentation. TBA value increased until 30 days, hereafter tended to decrease a little in the control during fermentation. TBA value increased until 30 days, hereafter tended to decrease a little in the control but it increased slowly until 40 days in cases of 0.02% BHT or 0.02% BHT with 0.05% carotenoid added. Fatty acids of fresh ascidian composed of polyenoic acid, saturated acid and monoenoic acid of 51.5%, 28.1% and 20.7%, respectively. Saturated fatty acids(C16:0, C14:0, C18:0) and monoenoic acids(C18:1, C16:1) increased while polyenoic acids(C20:5, C22:6) decreased during fermentation. Carotenoid was markedly degraded and discolored in the control during fermentation. But 0.02% BHT and 0.05% carotenoid treatments had bright color like fresh meat during 40 days. The results of sensory evaluation during the fermentation also convinced the retard of discoloration by the addition of BHT and carotenoid.