• KSBB Journal
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• v.14 no.3
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• pp.377-379
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• 1999

In order to improve the transfection efficiency of CHO/dhfr- cells using cationic lipid, optimal concentrations of the cationic lipid($LipofectAmine^{TM}$) and DNA(pEGFP-C1) need to be determined. The use of green fluorescent protein(GFP) gene as a reporter gene facilitated the quantification of transfection efficiency. The green fluorescence intensity of each cell transfected at various lipid-DNA concentrations was measured using fluorescence-activated cell sorter(FACS) analysis. A combination of $2.0{\mu}L$ cationic lipid and 0.4{$\mu}g$ DNA in a well resulted in the highest trasfection efficiency. Taken together, the method using FACS analysis of GFP is simple and fast, facilitating the optimization of transfection.

• Weed & Turfgrass Science
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• v.4 no.4
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• pp.383-389
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• 2015

The use of zoysiagrass (Zoysia japonica Steud.) in the transition zone is limited because of a lack of cold hardiness although zoysiagrass has many advantages compared to other warm-season and cool-season grasses. Late-fall N fertilization is often applied for darker green color of turfgrass in early spring and more extensive root growth without rapid top growth. The objective of the study was to evaluate the effects of late fall N application and turf cover for zoysiagrass spring green-up. Clear polyvinyl chloride (PVC) film was used for turf cover. The amount of N applied were 5 and $10g\;N\;m^{-2}$ for the low and high N rate treatments, respectively. Covered zoysiagrass had greater turfgrass color and quality in early spring than non-covered zoysiagrass. The high N rate had 0.6 to 2.3 greater turfgrass quality than the low N rate on 7 of 9 rating dates. Slow-release N as late fall fertilization is more effective for turfgrass color and quality than fast-release N in spring. Turf cover could reduce the period of yellow zoysiagrass, and the earlier time of spring green-up could be advanced by increasing turfgrass quality and growth of zoysiagrass.

• Weed & Turfgrass Science
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• v.7 no.3
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• pp.231-238
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• 2018

This study was carried out to develope new zoysiagrass cultivars 'JangsungChorok' (Application No. :2013-3) and 'JangsungSaetbyeol' (Application No. :2013-4). One hundred and one genetic resources were collected at Jangsung Gun area from 2010 to 2011. Among the collected lines, CY6097 (JangsungChorok) and CY6069 (JangsungSaetbyeol) showed superior performance in spring green up and growth rate. 'JangsungChorok' showed genetically light green leaf color, with medium leaf width (4.4 mm), height from ground to first leaf blade of 3.2 cm and average plant height of 18 cm. Fast recovery rate and early spring green up rate will make this cultivar (JangsungChorok) for efficient sod producer. 'JangsungSaetbyeol' showed genetically dark green leaf color, with fine to medium leaf width of 3.5 mm, height from ground to first leaf blade of 4.3 cm and average plant height of 22.6 cm. Fast recovery rate and early spring green up rate with relatively narrow leaf will make this cultivar (JangsungSaetbyeol) for high sod quality.

• Journal of Positioning, Navigation, and Timing
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• v.4 no.4
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• pp.161-171
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• 2015

Alternative binary offset carrier (AltBOC) signals can be approximated by four synchronized direct sequence spread spectrum (DSSS) signals, each pair of which is a quadrature phase shift keyed (QPSK) signal at a different frequency. Therefore, depending on the strength of an incoming AltBOC signal, an acquisition technique can reduce the mean acquisition time (MAT) by searching the four DSSS signals asynchronously; the search for each of the four DSSS signals can start at one of the evenly separated hypotheses on the two-dimensional hypothesis space. And detection sensitivity can be improved by multiple levels when different numbers of search results for the same hypothesis are combined. In this paper, we propose a fast AltBOC acquisition technique that has an asynchronous search strategy and efficiently utilizes the output of the four search results to increase the sensitivity level when sensitivity improvement is needed. We provide a complete theoretical analysis and demonstrate with numerous Monte Carlo simulations that the MAT of the proposed technique is much smaller than conventional AltBOC acquisition techniques.

• YAKHAK HOEJI
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• v.33 no.5
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• pp.308-311
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• 1989

Binding parameters of some edible dyes with bovine serum albumin were determined at pH 7.4 buffer solution. The edible dyes used were fast green, brilliant blue FCF, indigo carmine and allura red AC, and the final concentrations of each dyes were $3{\sim}9{\times}10^{-6}M$, $6{\times}10^{-6}M$, $3{\times}10^{-5}M$ and $3{\times}10^{-5}M$, respectively. The final concentrations of bovine serum albumin were $1{\sim}7{\times}10^{-5}M$. The values of binding free energy between edible dye and protein were ranged from -6,024 to -6,800 cal/mole.

• Journal of the Korean Ceramic Society
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• v.44 no.10
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• pp.568-573
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• 2007

For the manufacturing lightweight fine aggregate, clay and waste material was formed by pelletizer. The fine aggregate of 1-5 mm diameter was formed by diameter 76 cm pelletizer disc. Pelletization variables were : (1) pelletizer disc angle, (2) speed of revolution of pelletizer, (3) added pelletization time. Green and sintered aggregate were measured specific gravity, absorption rate and average size. The optimum condition were found that the pelletization variables were angle at $70^{\circ}$, speed of revolution of pelletizer at 23.2 rpm, and water/solid ratio at 1/5. At these conditions, it was formed that fine aggregate green whose average size was $2.0{\sim}3.35mm$. Specific gravity and average size are increased with low angle of disc and fast revolution speed of disc. Specific gravity and average size were not distinctly influenced by added pelletization time. Sintered aggregate was distinctly influenced by properties of green.

• Food Science of Animal Resources
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• v.35 no.3
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• pp.382-388
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• 2015

A duplex real-time polymerase chain reaction (PCR) based assay for the detection of porcine and horse meat in sausages was designed by using EvaGreen fluorescent dye. Primers were selected from mitochondrial 12S rRNA and 16S rRNA genes which are powerful regions for identification of horse and porcine meat. DNA from reference samples and industrial products was successfully extracted using the GIDAGEN® Multi-Fast DNA Isolation Kit. Genomes were identified based on their specific melting peaks (Mp) which are 82.5℃ and 78℃ for horse and porcine, respectively. The assay used in this study allowed the detection of as little as 0.0001% level of horse meat and 0.001% level of porcine meat in the experimental admixtures. These findings indicate that EvaGreen based duplex realtime PCR is a potentially sensitive, reliable, rapid and accurate assay for the detection of meat species adulterated with porcine and horse meats.

• Proceedings of the Korean Institute of Surface Engineering Conference
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• 2014.11a
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• pp.110-111
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• 2014

RGB laser 를 이용하여 rf-magnetron sputtering 법으로 합성한 a-IGZO 박막의 photoresponse 를 관찰하였다. Air 분위기에서 red 파장을 조사 할 경우 비교적 slow recovery 특성을 보였으며, green 과 blue 파장을 조사 할 경우 red 보다 fast recovery 특성을 나타내었다. 그러나 진공에서 측정할 경우, red 파장에서는 recovery 가 빨라졌으며, green 과 blue 파장의 경우 recovery 가 매우 느려짐을 확인하였다. 이는 passivation 을 하지 않은 소자의 oxygen gas 의 흡/탈착 때문으로 예상할 수 있었으며, red 파장이 gas 탈착에 기여하는 정도가 매우 작고, green 과 blue 파장이 gas 탈착에 기여하는 정도가 매우 크기 때문인 것으로 생각할 수 있었다. 온도를 증가시킬 경우, 모든 경우에서 recovery 가 빠르게 나타났는데 이는 흡/탈착에 필요한 barrier 및 $V_o{^{2+}}$에서 Vo 로 돌아오기 위한 barrier 를 쉽게 넘어갈 수 있기 때문으로 이해 할 수 있었다. 이러한 결과를 stretched exponential equation 을 이용하여 해석하였으며 수치화 하였다.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.33 no.3
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• pp.1173-1180
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• 2013

The construction industry has been a major player in Korean economy growth and it has been accountable for more than 15% of GDP during the last decade. However, fast-growing construction industry has also produced some unpleasant side-effects. Environmental problems are identified one of the main issues in the Korean construction industry and they are closely related to minimizing the carbon emission and delivering environmental-friendly green products. The movement of green construction, now, is considered the next driving force for the industry which has significant impacts on the future. This research investigates this green construction while focusing on governmental policy implications such as green certificate for construction products. Green building has long been recognized as the leading green construction product and the green trends have expanded to infrastructure construction such as road construction. Therefore, this research concentrates on various green road certification processes in the United States in order to develop a framework for Korean green road certification. In doing so, some of the green roads application has also been analyzed. This research intends to provide background information for Korean green construction policies, especially on green road certification.

• Journal of the Institute of Electronics and Information Engineers
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• v.50 no.2
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• pp.174-184
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• 2013

For the successful R&D and deployment of LED-based green energy lighting systems, the real-time power quality measurement of both various non-linear power signals including pulse waveform, spike waveform, etc and the undesired-signals including harmonics, sag, swell, etc is required. In this paper, we propose a low-cost power quality measurement (PQM) method for low- (60Hz-several KHz) to high-frequency (several tens KHz) power signals, which are generated by green-energy lighting systems, and implement a PQM testbed using TI TMS320F28335 MCU. The proposed algorithm is programmed using C in the CCS (Code Composer Studio) 3.3 environment and is verified using test signals generated by an arbitrary signal generator, NF-WF1974. In the implemented testbed, we can measure various non-linear current signals that LED SMPS generates, analyze harmonics by fast Fourier transform, and test sag, swell, and interruption using wavelet transform.