• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.219-219
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• 1996

Mucin release from hamster tracheal surface epithelial(HTSE) cells can be stimulated by extracellular ATP via activation of P$_2$ purinoceptors located on the cell surface which appears to be coupled to phospholipase C via G proteins. However, our preliminary data indicate that the ATP-induced mucin release involves, in part, activation of PKC, but not an increase in the intracellular Ca++ level, suggesting the presence of another pathway which is separate from the PLC-PKC pathway, In this study, we intended to confirm the previous observation and subsequently identify an additional mechanism. Confluent HTSE cells were metabolically labeled with either $^3$H-glucosamine or $^3$H-arachidonic acid(AA), and release of either $^3$H-mucin or $^3$H-AA was quantified following various treatments. $^3$H-mucin was assayed using the sepharose CL-4B gel-filtration method, whereas $^3$H-AA liberation was measured by counting $^3$H-radioactivity in the chase medium. We found that: (1)Desensitization of PKC by pretreatment with PMA completely abolished the mucin releasing effect of PMA but partially inhibited the ATP-induced mucin release; (2) ATP increases release of $^3$H-AA in a dose-dependent fashion; (3) mepacrine, an inhibitor of PLA$_2$, attenuates ATP-induced mucin release in a dose-dependent fashion. These results confirm our previous notion that the PLC-PKC pathway is responsible, in part, for ATP-induced mucin release. Furthermore, activation of PLA$_2$ appears to be an additional pathway which is involved in ATP-induced mucin release.

• 패션비즈니스
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• 제14권6호
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• pp.84-99
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• 2010

The aim of this study is to understand the wedding planners' general perceptions on Hanbok as a ceremonial dress in wedding ceremonies. Moreover, the study explores the aesthetic understanding of wedding planners on Hanbok which both influences their choice of recommendation and purchasing decisions. In the process of organizing the structure of weddings for clients, planners have naturally acquired the position of being able to influence the sales of the Hanbok industry. Moreover, brides-to-be are heavily dependant on wedding planners in sketching the outlines of their whole wedding. Therefore, in order to analyze the opinions of both subjects, a research survey was launched which involved three hundred participants from two groups: first, wedding planners who were free lancers working in the Chungdam District, and second, wedding planners who were working for wedding consulting firms. The survey method was based on one precedent study, in which a preliminary survey was conducted by interviewing companies in the Hanbok industry and wedding consulting firms, and an additional survey in the form of questionnaires. The STATA 11.0 program was used for analyzing the recruited data for frequency analysis and cross-tabulations analysis on career. The conclusion of this research may be used as substantial preliminary data in prospering the Hanbok industry, enhance and newly shape its marketing strategies and the conclusion is as follows. 1. Wedding planners have a more profound understanding on Hanbok and prioritize the significance of formative constituents in the order of color, material, style, size, and decoration. 2. The prospects of promoting the image of Hanbok through wedding planners is promising. According to surveys, this goes to show that the persuasion of wedding planners have brought a positive effect on choice of Hanbok and as wedding planners become more specialized, their involvement in preparing Hanbok with brides is increasing. 3. Wedding planners believed that the main reasons why future brides do not select Hanbok as a ceremony dress are that the cost is too high relative to practicality and that there are not enough opportunities to wear them.

• 한국의류학회지
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• 제35권6호
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• pp.678-686
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• 2011

Older people have increased interest and involvement in controlling their aging body through physical exercise and dieting. This study examined the relationship between body image and the participation of older people in exercise and dieting. Two measures of body image (appearance attitude and appearance orientation) were used to study their relationships to participation in exercise and dieting among older people. Data were collected via a mail survey of older married couples residing in three U.S. metropolitan areas of Florida. A total of 94 couples who were 60 years older participated in this study. Multiple regression was run separately for men and women. The results indicated that for both men and women, appearance attitude was inversely related to dieting and positively related with exercise (even though the relationship with exercise was weak for men). Appearance orientation was not significantly related to exercise or dieting for both men and women. A new scale development on body image for an aging population was suggested. Various activities performed by aging people to manage their aging body and appearance need to be studied as related to physical and psychological well-being that includes body image.

• The Korean Journal of Physiology and Pharmacology
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• 제6권4호
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• pp.187-191
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• 2002

Tamoxifen, an antiestrogen, has previously been shown to induce apoptosis in HepG2 human hepatoblastoma cells through activation of the pathways independent of estrogen receptors, i.e., intracellular $Ca^{2+}$ increase and generation of reactive oxygen species (ROS). However, the mechanism of tamoxifen to link increased intracellular $Ca^{2+}$ to ROS generation is currently unknown. Thus, in this study we investigated the possible involvement of calmodulin, a $Ca^{2+}$ activated protein, and $Ca^{2+}$/calmodulin-dependent protein kinase II in the above tamoxifen-induced events. Treatment with calmodulin antagonists (calmidazolium and trifluoroperazine) or specific inhibitors of $Ca^{2+}$/calmodulin-dependent protein kinase II (KN-93 and KN-62) inhibited the tamoxifen-induced apoptosis in a dose-dependent manner. In addition, these agents blocked the tamoxifen-induced ROS generation in a concentration-dependent fashion, which was completely suppressed by intracellular $Ca^{2+}$ chelation. These results demonstrate for the first time that, despite of its well-known direct calmodulin-inhibitory activity, tamoxifen may generate ROS and induce apoptosis through indirect activation of calmodulin and $Ca^{2+}$/calmodulin-dependent protein kinase II in HepG2 cells.

• Fisheries and Aquatic Sciences
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• 제20권12호
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• pp.31.1-31.11
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• 2017

Background: Liver-expressed antimicrobial peptide-2 (LEAP-2) is an important component of innate immune system in teleosts. In order to understand isoform-specific involvement and regulation of LEAP-2 genes in mud loach (Misgurnus mizolepis, Cypriniformes), a commercially important food fish, this study was aimed to characterize gene structure and expression characteristics of two paralog LEAP-2 isoforms. Results: Mud loach LEAP-2 isoforms (LEAP-2A and LEAP-2B) showed conserved features in the core structure of mature peptides characterized by four Cys residues to form two disulfide bonds. The two paralog isoforms represented a tripartite genomic organization, known as a common structure of vertebrate LEAP-2 genes. Bioinformatic analysis predicted various transcription factor binding motifs in the 5'-flanking regions of mud loach LEAP-2 genes with regard to development and immune response. Mud loach LEAP-2A and LEAP-2B isoforms exhibited different tissue expression patterns and were developmentally regulated. Both isoforms are rapidly modulated toward upregulation during bacterial challenge in an isoform and/or tissue-dependent fashion. Conclusion: Both LEAP-2 isoforms play protective roles not only in embryonic and larval development but also in early immune response to bacterial invasion in mud loach. The regulation pattern of the two isoform genes under basal and stimulated conditions would be isoform-specific, suggestive of a certain degree of functional divergence between isoforms in innate immune system in this species.

• 복식문화연구
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• 제12권6호
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• pp.984-998
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• 2004

The purpose of this study was to analyze contents of clothing product evaluation statements in the internet shopping mall and to discover consumers' recognition and evaluation of it. The data were collected from 151 consumers' statements in the four internet apparel shopping malls and a questionnaire was developed to measure consumers' recognition of clothing product evaluation statements. The questionnaire was administrated 272 consumers over 18 years old. The results of this study were as follows; First, The contents of clothing product evaluation statements in the internet shopping blog, were divided into three categories which were product, service and consumer buying decision process. Product category include evaluation of clothing product, description of difference between real product and product on the screen, and advice of uses. Service category include quickness and kindness in delivery, store preference for good service. For consumer buying decision process, motivation of purchasing, perceived risk, expression of satisfaction from good buying and canvassing for purchasing were included. Second, consumer recognized that clothing product evaluation statements has function of furnishing information, confirmation of purchasing decision, risk reduction of internet shopping, and interesting entertainment. Consumers also were dependent on product evaluation statements for making purchasing decision. And it was trusted by consumers. This recognition & evaluation were correlated with consumer's clothing product knowledge, involvement, risk perception of internet shopping, experiences of internet shopping. Managerial implications are provided for internet shopping mall.

• Fashion, Industry and Education
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• 제15권2호
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• pp.36-52
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• 2017

In this study, we tried to understand preschooler clothing trends and identify the purchase behaviors of various consumers, such as grandparents, aunts, uncles, and parents, who are the main consumers of the preschooler clothing market. We conducted in-depth interviews with consumers who were interested in preschooler clothing, purchased it frequently, and bought preschooler clothing within the last three months. Their purchase motivations and purchase behaviors were analyzed through exploratory and inductive analysis. The analysis was conducted by integrating categories into one process centered on core categories through open coding, axial coding, and selective coding. The results of this study included that 1) a variety of clothing purchase behaviors appeared as a central phenomenon, 2) the causal conditions that contributed to this phenomenon were the buyers' childcare experiences, purchase experiences, the personal consumption values of individuals, and income levels, 3) in response to the central phenomenon, the parents' action/interaction strategies were acceptance and rejection of purchased clothing, 4) contextual and Intervening conditions affecting action/interaction strategy were family environment changes, the VIB (Very Important Baby) phenomenon, parents' clothing involvement, and the relationship with the buyers, and acceptance by the users, and 5) as a result of the strategy expressed as acceptance and rejection, various clothing behaviors emerged. In this study, we identified that there are various influencers, apart from parents, involved in children's clothing consumption. Therefore, we need to keep in mind that various purchase behaviors and clothing trends that appear during one's childhood may affect the individual's clothing behavior in the future.

• 대한가정학회지
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• 제39권11호
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• pp.107-128
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• 2001

The objectives of this study were to classify the consumer group based on the lifestyle traits of cortege women, to examine the demographic characteristics of each group, and to find out the differences of buying and using behavior of cosmetics among each group. The survey was conducted and 392 samples were analyzed by SPSS package program. The results of this study were as follows; 1. The lifestyle types were clasified 4 groups. They were named 'Progressive fashion pursuing type' 'Reality adaptable living-satisfaction type','Traditional frugal faithful type','Passive living-unconcern type'. 2. The demographic characteristics of each lifestyle group showed meaningful differences according to the following variables ; major, average monthly pocket money, religion, living standard, educational level and occupation of the head of a family. 3. The buying behavior of cosmetics(the degree of product involvement, information search behavior, product choice criteria, store choice criteria) showed meaningful differences among each lifestyle group. 4. The using behavior of cosmetics( the motive of make-up, the extent of make-up, average monthly cosmetics expenditure) showed meaningful differences among each lifestyle group.

• Bulletin of the Korean Chemical Society
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• 제5권3호
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• pp.121-126
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• 1984

The charge transfer complex formations between indole derivatives and methylviologen were investigated spectroscopically. In aqueous solutions near room temperature, the order of complex stability was tryptamine < tryptophan < indole < indole acetate, which is the reverse order of the magnitude of molar absorptivities. This was interpreted as involvement of contact charge transfer. The decrease of enthalpy of complex formation (-${\Delta}$H) was highest in tryptamine, and lowest in indole acetate. ${\Delta}$H and entropy of complex formation (${Delta}$S) varied nearly in a linear fashion with isokinetic temperature $242^{\circ}$K. These results were attributed to the hydration-dehydration properties of the side chains in indole derivatives. Except indole acetate, the complex formations were greatly enhanced by the addition of sodium dodecyl sulfate(SDS). However, the direct relationship between the enhanced complex formation and SDS micelle formation was not found. The enhanced charge transfer interaction inSDS solutions was attributed to the increased ${\Delta}$S by interaction between methylviologen and SDS in premicellar level. The order of complex stability in SDS solutions was indole acetate < tryptophan < trypamine < indole, which reflects the hydrophobicity of indole derivatives as well as electrostatic interaction between indole derivatives and methylviologen associated with SDS.

• 한국동물학회지
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• 제30권2호
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• pp.117-139
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• 1987

The present experiments were carried out to investigate the mode of cAMP regulation of cumulus expansion in pig. Intracellular level of cAMP in the cumulus cells was modulated by culturing porcine cumulus oocyte complexes (COC's) with forskolin, an adenylate cyclase stimulator and 3-isobutyl-1-methylxanthine (IBMX), a phosphodiesterase inhibitor. The role of calcium in the hormone induced cumulus expansion process was also studied. Forskolin in the medium stimulated cumulus expansion from the concentration of 0.01 $\mu$M and induced full expansion at l-10 $\mu$M In contrast, IBMX in the medium (20-180 $\mu$M) failed to induce the expansion. Verapamil, a calcium ion transport blocker, suppressed follicle stimulating hormone(FSH)-induced cumulus expansion in a dose dependent fashion (0.002-0. 2 mM) when the COC's were exposed to the drugs during culture period (32 hr). But verapamil did not interfere with the triggering action of FSH during early four hours of culture period. The data presented here showed that adenylate cyclase in the porcine cumulus cells may play a key role in the regulation of the intracellular cAMP level and calcium ion may be involved in the later period of cumulus expansion process.