• 한국치위생학회지
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• 제14권3호
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• pp.363-370
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• 2014

Objectives : The purpose of the study is to investigate the effect of the oral hygiene care program in stroke patients. Methods : The subjects were 62 stroke patients of rehabilitation department in Youngnam University Hospital in Daegu. The study was carried out from January, 2010 to October, 2010. Intervention group was 35 and the control group was 31 patients. Tooth brushing instruction, oral health education and scaling were implemented in all patients at baseline. This program was conducted 5 times during hospitalization in intervention group. Results : In the intervention group, the mean age was 59.2 years, and women accounted for 66.7% and the control group consisted of 72.4% of women and mean age was 58.5 years. The average of the number of permanent teeth with experience of dental caries, the values of dental calculus index, plaque index, bleeding index and tooth mobility were 11.88, 11.79, 9.91, 3.16 and 1.16, respectively. The control group showed 10.21, 11.79, 9.91, 3.16 and 1.16, respectively. The proportion of subjects whose CPI was higher than 3 accounted for 97%, and the O'Leary index score was 38.6 in the intervention grou. The control group showed 100% and 38.79. Conclusions : Oral condition improved significantly in the intervention group in the third session. These findings may provide essential information for oral health program planning and implementation.

• Molecules and Cells
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• 제23권1호
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• pp.30-38
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• 2007

Dipyrithione (2, 2'-dithiobispyridine-1, 1'-dioxide, PTS2), a pyrithione derivate, is highly bactericidal and fungicidal. In this study we examined its apoptotic effect on HeLa cells. PTS2 induced HeLa cell death in a dose and time dependent manner. ERK1/2 and p38 were markedly activated, but little JNK1/2 activation was detected. Suppression of p38 activation by SB203580 reduced the extent of apoptosis of the HeLa cells and also prevented induction of p21, release of cytochrome c, and cleavage of caspase-3 and PARP. Inhibition of ERK1/2 with PD98059 increased apoptosis, indicating that ERK1/2 activation has an anti-apoptotic effect on PTS2-induced HeLa cell apoptosis. PTS2 also inhibited murine sarcoma 180 and hepatoma 22 tumor growth in an animal tumor model. Our findings indicate that PTS2 possesses anti-tumor activity, that caspase-3 and poly (ADP-ribose) polymerase (PARP) are involved in PTS2-induced HeLa cell apoptosis and that ERK1/2 and p38 have opposing effects on this apoptosis.

• Structural Engineering and Mechanics
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• 제76권2호
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• pp.153-162
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• 2020

Aiming at the mechanical and structural characteristics of the contact zone composite rock, the shear tests and numerical studies were carried out. The effects of the differences in mechanical properties of different materials and the normal stress on shear properties of contact zone composite samples were analyzed from a macro-meso level. The results show that the composite samples have high shear strength, and the interface of different materials has strong adhesion. The differences in mechanical properties of materials weakens the shear strength and increase the shear brittleness of the sample, while normal stress will inhibit these effect. Under low/high normal stress, the sample show two failure modes, at the meso-damage level: elastic-shearing-frictional sliding and elastic-extrusion wear. This is mainly controlled by the contact and friction state of the material after damage. The secondary failure of undulating structure under normal-shear stress is the nature of extrusion wear, which is positively correlated to the normal stress and the degree of difference in mechanical properties of different materials. The increase of the mechanical difference of the sample will enhance the shear brittleness under lower normal stress and the shear interaction under higher normal stress.

• KIEAE Journal
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• 제17권1호
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• pp.83-89
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• 2017

Purpose: Thermal sensation or thermal comfort was randomly used in many studies which focused on combined effects of thermal and acoustic environments on human perception. However, thermal sensation and thermal comfort are not synonyms. Thermal comfort is more complex human perception on thermal environment than thermal sensation. This study aims to investigate effects of noise on thermal sensation and thermal comfort separately, and also to investigate effects of temperature on acoustic sensation and comfort. Method: Combined thermal and acoustic configurations were simulated in an indoor environmental chamber. Twenty four participants were exposed to two types of noise (fan and babble) with two noise levels (45 dBA and 60 dBA) for an hour in each thermal condition of PMV-1.53, 0.03, 1.53, 1.83, respectively. Temperature sensation, temperature preference, thermal comfort, noisiness, loudness, annoyance, acoustic comfort, indoor environmental comfort were evaluated in each combined environmental condition. Result: Noise did not affected thermal sensation, but thermal comfort significantly. Temperature had an effect on acoustic comfort significantly, but no effect on noisiness and loudness in overall data analysis. More explicit interactions between thermal condition and noise perception showed only with the noise level of 60 dBA. Impacts of both thermal comfort and acoustic comfort on the indoor environmental comfort were analyzed. In adverse thermal environments, thermal comfort had more impact than acoustic comfort on indoor environmental comfort, and in neutral thermal environments, acoustic comfort had more important than thermal comfort.

• Smart Structures and Systems
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• 제20권2호
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• pp.127-137
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• 2017

Identification of damping characteristics is of significant importance for dynamic response analysis and condition assessment of structural systems. Damping is associated with the behavior of the energy dissipation mechanism. Identification of damping ratios based on the sensitivity of dynamic responses and the model updating technique is investigated with numerical and experimental investigations. The effectiveness and performance of using the sensitivity-based model updating method and vibration monitoring data for damping ratios identification are investigated. Numerical studies on a three-dimensional truss bridge model are conducted to verify the effectiveness of the proposed approach. Measurement noise effect and the initial finite element modelling errors are considered. The results demonstrate that the damping ratio identification with the proposed approach is not sensitive to the noise effect but could be affected significantly by the modelling errors. Experimental studies on a steel planar frame structure are conducted. The robustness and performance of the proposed damping identification approach are investigated with real measured vibration data. The results demonstrate that the proposed approach has a decent and reliable performance to identify the damping ratios.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6369-6374
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• 2012

Vitexicarpin (3', 5-dihydroxy-3, 4', 6, 7-tetramethoxyflavone), a polymethoxyflavone isolated from Viticis Fructus (Vitex rotundifolia Linne fil.), has long been used as an anti-inflammatory herb in traditional Chinese medicine. It has also been reported that vitexicarpin can inhibit the growth of various cancer cells. However, there is no report elucidating its effect on human prostate carcinoma cells. The aim of the present study was to examine the apoptotic induction activity of vitexicarpin on PC-3 cells and molecular mechanisms involved. MTT studies showed that vitexicarpin dose-dependently inhibited growth of PC-3 cells with an $IC_{50}{\sim}28.8{\mu}M$. Hoechst 33258 staining further revealed that vitexicarpin induced apoptotic cell death. The effect of vitexicarpin on PC-3 cells apoptosis was tested using prodium iodide (PI)/Annexin V-FITC double staining and flow cytometry. The results indicated that vitexicarpin induction of apoptotic cell death in PC-3 cells was accompanied by cell cycle arrest in the G2/M phase. Furthermore, our study demonstrated that vitexicarpin induction of PC-3 cell apoptosis was associated with upregulation of the proapoptotic protein Bax, and downregulation of antiapoptotic protein Bcl-2, release of Cytochrome c from mitochondria and decrease in mitochondrial membrane potential. Our findings suggested that vitexicarpin may become a potential leading drug in the therapy of prostate carcinoma.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6517-6521
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• 2012

To analyze the growth, proliferation, apoptosis, invasiveness and chemotherapy sensitivity of EC9706 cells after K-Ras gene silencing, an expression carrier pSilencer-siK-Ras was constructed, and the EC9706 cell line was transfected using a liposome technique. Six groups were established: Control, siRNA NC (transfected with empty vector pSilencer2.1); Ras siRNA (transfected with pSilencer-siK-Ras2); Paclitaxel; Paclitaxel + siRNA NC; and Ras siRNA + Paclitaxel. After the treatment, RT-PCR, Western blotting, MTT assay, flow cytometry and the Transwell technique were used to assess expression of K-Ras mRNA and protein in EC9706 cells, as well as cell growth, proliferation, apoptosis and invasiveness. The effect of Paclitaxel chemotherapy was also tested. pSilencer-siK-Ras2 effectively down-regulated expression of K-Ras mRNA and protein in EC9706 cells, growth being significantly inhibited. Flow cytometry indicated obvious apoptosis of cells in the experimental group, with arrest in the G1 phase; cell migration ability was also reduced. After pSilencer-siK-Ras2 transfection or the addition of Paclitaxel, EC9706 cells were suppressed to different extents; the suppressive effect was strengthened by combined treatment. The results suggested that RNAi-induced K-Ras gene silencing could enhance chemotherapy sensitivity of esophageal cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제15권18호
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• pp.7687-7692
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• 2014

Aim: To observe the effects of a novel all-trans retinoid acid (ATRA) derivative, N-(3-trifluoromethyl-phenyl)-retinamide (ATPR), on lung adenocarcinoma A549 cells and to explore the potential mechanism of ATPR inhibiting of A549 cell migration. Materials and Methods: The cytotoxicity of ATRA and ATPR on A549 cells was assessed using MTT assay. Wound healing assays were used to analyze the influences of ATRA, ATPR, ML-7 (a highly selective inhibitor of myosin light chain kinase (MLCK)), PMA (an activator of MAPKs) and PD98059 (a selective inhibitor of ERK1/2) on the migration of A549 cells. Expression of MLCK and phosphorylation of myosin light chain (MLC) were assessed by Western blotting. Results: ATRA and ATPR inhibited the proliferation of A549 cells in a dose- and time-dependent manner, and the effect of ATPR was much more remarkable compared with ATRA. Relative migration rate and migration distance of A549 cells both decreased significantly after treatment with ATPR or ML-7. The effect on cell migration of PD98059 combining ATPR treatment was more notable than that of ATPR alone. Moreover, compared with control groups, the expression levels of MLCK and phosphorylated MLC in A549 cells were both clearly reduced in ATRA and ATPR groups. Conclusions: ATPR could suppress the migration and invasion of A549 cells, and the mechanism might be concerned with down-regulating the expression of MLCK in the ERK-MAPK signaling pathway, pointing to therapeutic prospects in lung cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제15권20호
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• pp.8775-8778
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• 2014

Background: To explore the relationship between polymorphisms of interleukin17 (IL-17) gene(A-832G 7488A/G) and the susceptibility to silicosis, a risk factor for lung cancer. Materials and Methods: A total of 113 silicosis patients and 116 workers without silicosis were enrolled in the case-control study. IL-17A A-832G and IL-17F 7488A/G polymorphisms were evaluated by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: The frequencies of AA,GG and AG of IL-17A A-832G locus in the case and control groups were 46.9%, 8.0%, 45.1%, and 49.2%, 7.6%, 43.2%, respectively, with no significant differences (p>0.05).The GG genotype in the IL-17F (7488A/G) locus was not found. The frequencies of AA and GA of IL-17F 7488A/G locus in the case and control groups were 84.1%, 15.9% and 66.4%, 33.6%, respectively (p<0.05). Analysis of combined effects showed that the individuals with GG+AG genotype of IL-17A and GG+GA genotype of IL-17F are protected against silicosis (OR=0.469). Conclusions: IL-17F 7488A/G is associated with susceptibility to silicosis, and G allele may have a protective effect. No relationship was found between IL-17A gene polymorphisms at A-832G and silicosis.

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1182-1185
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• 2002

The objective of this study was to investigate the effect of long light regime (20 h light and 4 h dark, 20L:4D) and short light regime (4 h light and 20 h dark, 4L:20D) on egg production and egg characteristics of laying geese. Thirty-six laying birds, 4 replicates of 3 birds per treatment were allotted to three light regimes, i.e., 20L:4D, 4L:20D, and natural light (NAT) from March 7 to June 20. Results showed that the geese in 20L:4D consumed 54 g less feed per goose daily and laid 17.5 less eggs per goose (p<0.05) comparing to those in 4L:20D. The number of days from initiation of light treatment till cease of laying was 22 days shorter (p<0.05) in 20L:4D comparing to that in NAT. Five geese (41.7%) in 4L:20D kept laying by the end of applying light regime. Weight and surface area of the eggs in 4L:20D were greater (p<0.05) comparing to those in the other two light regimes. It is concluded that the period of egg production in goose could be manipulated by light regime in the ways such as using short light regime of 4 h light daily to prolong egg production through summer and using long light regime of 20 h light daily to induce cease of egg production.