• Nutrition Research and Practice
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• 제14권2호
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• pp.127-133
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• 2020

BACKGROUND/OBJECTIVES: Non-small cell lung cancer is mostly recognized among other types of lung cancer with a poor prognosis by cause of chemotherapeutic resistance and increased metastasis. Luteolin has been found to decrease cell metastasis. However, its underlying mechanisms remain unresolved. The objective of this study was to examine the effect (and its mechanism) of luteolin on the migration and invasion of human non-small cell lung cancer A549 cells. MATERIALS/METHODS: Cell viability was investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. Wound healing and transwell assays were evaluated to assess migration and invasion, respectively. Western blot analysis and immunofluorescence were further performed to investigate the role of luteolin and its mechanisms of action. RESULTS: Administration with up to 40 μM luteolin showed no cytotoxic activity on lung cancer A549 cells or non-cancer MRC-5 cells. Additionally, luteolin at 20-40 μM significantly suppressed A549 cells' migration, invasion, and the formation of filopodia in a concentration-dependent manner at 24 h. This is similar with western blot analysis, which revealed diminished the phosphorylated focal adhesion kinase (pFAK), phosphorylated non-receptor tyrosine kinase (pSrc), Ras-related C3 botulinum toxin substrate 1 (Rac1), cell division control protein 42 (Cdc42), and Ras homolog gene family member A (RhoA) expression levels. CONCLUSIONS: Overall, our data indicate that luteolin plays a role in controlling lung cancer cells' migration and invasion via Src/FAK and its downstream Rac1, Cdc42, and RhoA pathways. Luteolin might be considered a promising candidate for suppressing invasion and metastasis of lung cancer cells.

• 한국과학교육학회지
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• 제11권2호
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• pp.143-159
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• 1991

The purpose of this study is to develop a data base system for the infromation on research and development of science education. As a part of this development, an analysis of master's theses and doctoral dissertations from the Department of Science Education, Seoul National University was done, and using authors' classification system for the research and development materials of science education, those theses were classified. From the analysis and classification, the following conclusion was drawn: 1) The Department of Science Education, Seoul National University had produced 468 masters of education for about 30 years. Among them, only 107 theses were on the science education and the other 361 theses on the pure science. This means that department has not taken root as a department of science education. If it does not carry out its own purpose of establishment, it will not be able to justify its existence any longer 2) As compared to the increased number of students applying for the doctoral program, the number of faculty is very few in the field of science education. Without more supplement of the faculty member majored science education, there will be increased conflict and disorder among faculty and students. 3) The proportion of the theses on science education to those on science vanes greatly by the major of the department. This is the mirror of the faculty attitude to and recognition of science education and the faculty composition. 4) The classification of master theses and doctoral dissertations on science education showed that most of them focused on the secondary school science education and were survey studies. But recently it is noticeable that the theme of the study became diversified and has kept in step with the international research trends.

• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3053-3059
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• 2012

Human epidermal growth factor receptor 2 (HER2) is a member of the epidermal growth factor receptor family of receptor tyrosine kinases that plays important roles in all processes of cell development. Their overexpression is related to many cancers, including examples in the breast, ovaries and stomach. Anticancer therapies targeting the HER2 receptor have shown promise, and monoclonal antibodies against subdomains II and IV of the HER2 extra-cellular domain (ECD), Pertuzumab and Herceptin, are currently used in treatments for some types of breast cancers. Since anti HER2 antibodies targeting distinct epitopes have different biological effects on cancer cells; in this research linear and conformational B cell epitopes of HER2 ECD, subdomain III, were identified by bioinformatics analyses using a combination of linear B cell epitope prediction web servers such as ABCpred, BCPREDs, Bepired, Bcepred and Elliprro. Then, Discotope, CBtope and SUPERFICIAL software tools were employed for conformational B cell epitope prediction. In contrast to previously reported epitopes of HER2 ECD we predicted conformational B cell epitopes $P1_C$: 378-393 (PESFDGDPASNTAPLQ) and $P2_C$: 500-510 (PEDECVGEGLA) by the integrated strategy and P4: PESFDGD-X-TAPLQ; P5: PESFDGDP X TAPLQ; P6: ESFDGDP X NTAPLQP; P7: PESFDGDP-X-NTAPLQ; P8: ESFDG-XX-TAPLQPEQL and P9: ESFDGDP-X-NTAPLQP by SUPERFICIAL software. These epitopes could be further used as peptide antigens to actively immune mice for development of new monoclonal antibodies and peptide cancer vaccines that target different epitopes or structural domains of HER2 ECD.

• Earthquakes and Structures
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• 제18권4호
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• pp.407-421
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• 2020

In-place analysis for offshore platforms is essentially required to make proper design for new structures and true assessment for existing structures, in addition to the structural integrity of platforms components under the maximum and minimum operating loads when subjected to the environmental conditions. In-place analysis have been executed to check that the structural member with all appurtenance's robustness have the capability to support the applied loads in either storm or operating conditions. A nonlinear finite element analysis is adopted for the platform structure above the seabed and pile-soil interaction to estimate the in-place behavior of a typical fixed offshore platform. The SACS software is utilized to calculate the dynamic characteristics of the platform model and the response of platform joints then the stresses at selected members, as well as their nodal displacements. The directions of environmental loads and water depth variations have significant effects in the results of the in-place analysis behavior. The most of bending moment responses of the piles are in the first fourth of pile penetration depth from pile head level. The axial deformations of piles in all load combinations cases of all piles are inversely proportional with penetration depth. The largest values of axial soil reaction are shown at the pile tips levels (the maximum penetration level). The most of lateral soil reactions resultant are in the first third of pile penetration depth from pile head level and approximately vanished after that penetration. The influence of the soil-structure interaction on the response of the jacket foundation predicts that the flexible foundation model is necessary to estimate the force responses demands of the offshore platform with a piled jacket-support structure well.

• 대한약침학회지
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• 제22권1호
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• pp.16-27
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• 2019

Generative and vegetative parts of the cactuses have had a long-lasting position in folk medicine and their effects could partly be confirmed in scientific experiments. Nowadays, the cactus, fruits, and cladodes are the focus of many studies because of their desirable properties. Therefore, the summarized reports of valuable properties of medicinal plants may be a good way to familiarize researches with a new source of drugs with lower side effects and higher efficacy. Opuntia dillenii, a well-known member of the Cactaceae family, is used as a medicinal plant in various countries and grows in the desert, semi-desert, tropical and sub-tropical areas. It shows diverse pharmacological activities such as: antioxidant, anti-inflammatory, anti-tumor, neuroprotective, hepatoprotective, hypotensive etc. OD fruit also possesses valuable constitutes for instance: betalains, ascorbic acid, total phenol, protein as well as essential elements which suggest the significant potential of this plant as a complementary therapy against several pathological conditions. This review describes experimental evidence about pharmacological and therapeutic potential of OD in order to give the basis of its application in the prevention and treatment of some chronic diseases. More studies on OD can help better understanding of its pharmacological mechanism of action to explain its traditional uses and to identify its potential new therapeutic applications.

• Coupled systems mechanics
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• 제11권6호
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• pp.505-524
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• 2022

Floods represent extreme hydrological phenomena that affect populations, environment, social, political, and ecological systems. After the catastrophic floods that have hit Europe and the World in recent decades, the flood problem has become more current. At the EU level, a legal framework has been put in place with the entry into force of Directive 2007/60/EC on Flood Risk Assessment and Management (Flood Directive). Two years after the entry into force of the Floods Directive, Bosnia and Herzegovina (B&H), has adopted a Regulation on the types and content of water protection plans, which takes key steps and activities under the Floods Directive. The "Methodology for developing flood hazard and risk maps" (Methodology) was developed for the territory of Bosnia and Herzegovina, following the methodology used in the majority of EU member states, but with certain modifications to the country's characteristics. Accordingly, activities for the preparation of the Preliminary Flood Risk Assessment for each river basin district were completed in 2015 for the territory of Bosnia and Herzegovina. Activities on the production of hazard maps and flood risk maps are in progress. The results of probable climate change impact model forecasts should be included in the preparation of the Flood Risk Management Plans, which is the subsequent phase of implementing the Flood Directive. By the foregoing, the paper will give an example of the development of the hydrodynamic model of the Zujevina River, as well as the development of hazard and risk maps. Hazard and risk maps have been prepared for medium probability floods of 1/100 as well as for high probability floods of 1/20. The results of LiDAR (Light Detection and Ranging) recording were used to create a digital terrain model (DMR). It was noticed that there are big differences between the flood maps obtained by recording LiDAR techniques in relation to the previous flood maps obtained using georeferenced topographic maps. Particular attention is given to explaining the Methodology applied in Bosnia and Herzegovina.

• Earthquakes and Structures
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• 제22권3호
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• pp.245-261
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• 2022

Key questions to researchers interested in nonlinear analysis of skeletal structures are whether the distributed plasticity approach - albeit computationally demanding - is more reliable than the concentrated plasticity to adequately capture the extent and severity of the inelastic response, and whether force-based formulation is more efficient than displacement-based formulation without compromising accuracy. The present research focusing on performance-based seismic response of mid-span concrete bridges provides a pilot holistic investigation opting for some hands-on answers. OpenSees software is considered adopting different modeling techniques, viz. distributed plasticity (through either displacement-based or force-based elements) and concentrated plasticity via beam-with-hinges elements. The pros and cons of each are discussed based on nonlinear pushover analysis results, and fragility curves generated for various performance levels relying on incremental dynamic analyses under real earthquake records. Among prime conclusions, distributed plasticity modeling albeit inherently not relying on prior knowledge of plastic hinge length still somewhat depends on such information to ensure accurate results. For instance, displacement-based and force-based approaches secure optimal accuracy when dividing, for the former, the member into sub-elements, and satisfying, for the latter, a distance between any two consecutive integration points, close to the expected plastic hinge length. On the other hand, using beam-with-hinges elements is computationally more efficient relative to the distributed plasticity, yet with acceptable accuracy provided the user has prior reasonable estimate of the anticipated plastic hinge length. Furthermore, when intrusive performance levels (viz. life safety or collapse) are of concern, concentrated plasticity via beam-with-hinges ensures conservative predicted capacity of investigated bridge systems.

• 대한건축학회논문집:계획계
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• 제34권8호
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• pp.59-67
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• 2018

In a state where ancient buildings was left a lot in the case of Korea, many parts do not remain the documentation, and there is a Kiwarisho lot in Japan, however, the present methods and tree split does not exist in the Korea. However, it is impossible to make a perfect building without splitting exact member when making a building. Thus will not be revealed simply by presumption that there is a similar way to wood comparatively far in Korea. Without a comparison of building dimensions and Korea calculation dimensions Kiwarisho In the first study about the proportionality of Korea, I do not know until now, other studies of the following documents which house a high approximation ratio and member size of Korea consider reason to have is impossible. View compared with respect to the axis of the building in the early stages of research first. In the case of Japan and Korea to form a tree frames in basic form that form the building, the shaft portion is easy to understand and easy compared in common is at most the shaft portion.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7091-7095
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• 2014

Background: The study aimed to evaluate the incidence of CpG island promoter methylation of BMP6, a member of the transforming growth factor beta family, in tissue samples from colorectal cancers (CRC) and look for its association with BMP6 expression and clinicopathological correlation. Materials and Methods: Methylation specific PCR for the BMP6 promoter region was performed with 85 frozen tissue samples of CRC and 45 of normal colon. Methylation status of MLH1 was also determined by the same method. Expression of BMP6 was evaluated by immunohistochemistry (IHC), using Allred's scoring system. The methylation status was analyzed against clinical and pathological parameters in CRC. Results: The study revealed BMP6 hypermethylation in 34 of 85 tumor specimens (40%), and 15 out of 45 normal tissue samples from CRC (33%). The incidence of hypermethylation was inversely correlated with IHC score. Allred's scores of 7 or more were correlated with lower frequency of BMP6 hypermethylation (29% compared to 50% in the remaining, p-value 0.049). However, there was no association between hypermethylation status and any clinicopathological parameters. The methylation status of BMP6 was not correlated with that of MLH1, a key methylation determinant in CRC. On survival analysis, there was no significant difference in progress-free survival (PFS) between the cases with and without hypermethylation (2-year PFS 74% and 76%, respectively). Conclusions: CpG island methylation of BMP6 is found in high frequency in CRC and this epigenetic event is associated with suppressed protein expression in the tumor tissue. However, the marker is not associated with tumor progression of the disease.

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.2013-2020
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• 2014

Background: The aldehyde dehydrogenase 1 family member A1 (ALDH1A1) is one of the promising markers for identifying cancer stem cells in many cancer types, along with other markers including CD44. The aim of the present study was to evaluate the expression and clinical significance of putative cancer stem cell markers, CD44 and ALDH1A1, in a series of urothelial carcinomas of urinary bladder (UCUB) by tissue microarray (TMA). Materials and Methods: A total of 159 Urothelial Carcinomas (UC) including 96 (60%) low grade and 63 (40%) high grade carcinomas were immunohistochemically examined for the expression of CD44 and ALDH1A1. Correlations of the relative expression of these markers with clinicopathological parameters were also assessed. Results: High level expression of ALDH1A1 was found in 16% (25/159) of bladder UC which was significantly correlated with increased tumor size (p value=0.002), high grade (p value<0.001), pathologic stage (T1, p value=0.007 and T2, p value<0.001) and increased rate of recurrence (p value=0.013). A high level of CD44 expression was found in 43% (68/159) of cases, being positively correlated with histologic grade (p value=0.032) and recurrence (p value=0.039). Conclusions: Taken together, our results showed that ALDH1 was concurrently expressed in a fraction of CD44+ tumors and its expression correlated with poor prognosis in UCs. ALDH1A1 could be an ideal marker for targeted therapy of UCs in combination with conventional therapies, particularly in patients with high grade carcinomas. These findings indicate that cells expressing ALDH1A1 along with CD44 can be a potential therapeutic target in bladder carcinomas.