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Overcoming taxonomic challenges in DNA barcoding for improvement of identification and preservation of clariid catfish species

  • Piangjai Chalermwong;Thitipong Panthum;Pish Wattanadilokcahtkun;Nattakan Ariyaraphong;Thanyapat Thong;Phanitada Srikampa;Worapong Singchat;Syed Farhan Ahmad;Kantika Noito;Ryan Rasoarahona;Artem Lisachov;Hina Ali;Ekaphan Kraichak;Narongrit Muangmai;Satid Chatchaiphan6;Kednapat Sriphairoj;Sittichai Hatachote;Aingorn Chaiyes;Chatchawan Jantasuriyarat;Visarut Chailertlit;Warong Suksavate;Jumaporn Sonongbua;Witsanu Srimai;Sunchai Payungporn;Kyudong Han;Agostinho Antunes;Prapansak Srisapoome;Akihiko Koga;Prateep Duengkae;Yoichi Matsuda;Uthairat Na-Nakorn;Kornsorn Srikulnath
    • Genomics & Informatics
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    • 제21권3호
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    • pp.39.1-39.15
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    • 2023

  • DNA barcoding without assessing reliability and validity causes taxonomic errors of species identification, which is responsible for disruptions of their conservation and aquaculture industry. Although DNA barcoding facilitates molecular identification and phylogenetic analysis of species, its availability in clariid catfish lineage remains uncertain. In this study, DNA barcoding was developed and validated for clariid catfish. 2,970 barcode sequences from mitochondrial cytochrome c oxidase I (COI) and cytochrome b (Cytb) genes and D-loop sequences were analyzed for 37 clariid catfish species. The highest intraspecific nearest neighbor distances were 85.47%, 98.03%, and 89.10% for COI, Cytb, and D-loop sequences, respectively. This suggests that the Cytb gene is the most appropriate for identifying clariid catfish and can serve as a standard region for DNA barcoding. A positive barcoding gap between interspecific and intraspecific sequence divergence was observed in the Cytb dataset but not in the COI and D-loop datasets. Intraspecific variation was typically less than 4.4%, whereas interspecific variation was generally more than 66.9%. However, a species complex was detected in walking catfish and significant intraspecific sequence divergence was observed in North African catfish. These findings suggest the need to focus on developing a DNA barcoding system for classifying clariid catfish properly and to validate its efficacy for a wider range of clariid catfish. With an enriched database of multiple sequences from a target species and its genus, species identification can be more accurate and biodiversity assessment of the species can be facilitated.

  • https://doi.org/10.5808/gi.23038 인용 PDF

FLUORESCENCE PROPERTIES OF POLYCYANOANILINES SYNTHESIZED FROM ELECTROPHILIC ETHYLENES AND MALONONITRILE DERIVATIVES

  • Nakano, Yoshiharu;Ishiwata, Tetsuya;Yamaguchi, Satoshi;Toki, Masanori;Igarashi, Minoru;Usui, Yoshiharu;Miyamae, Hiroshi;Yamada, Yoichi;Yasuda, Heinosuke
    • Journal of Photoscience
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    • 제6권4호
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    • pp.193-198
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    • 1999

  • Various arylidene- or alkylidenemalononitriles are condensed with electrophilic ethylenes to obtain polycyanoaniline derivatives. All the anilines showed strong fluorescence and the fluorescence intensities was evaluated to the effect of substituents.

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Anticancer Effects in ECT for the Mechanism

  • Gu Yeunhwa;Tanabe Hiroaki;Yamashita Takenori;Maenaka Toshihiro;Saito Kiyoto;Hasegawa Takeo;Terai Kaoru;yuicti Mituhana;Bamen Kenichi;Choi Ihll Bong
    • 한국방사성폐기물학회:학술대회논문집
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    • 한국방사성폐기물학회 2004년도 학술대회지
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    • pp.204.2-204.2
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    • 2004

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