• Microbiology and Biotechnology Letters
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• v.31 no.2
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• pp.171-176
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• 2003

Productivity of biosurfactant (rhamnolipid) by Pseudomonas aeuginosa F722 was investigated in the several culture conditions and culture composition. Biosurfactant production by P. aeuginosa F722 was amounted to 0.78 g/l as the result of the nitrogen sources and carbon sources without investing of optimum conditions. As for that one was investigated, biosurfactant production by P. aeruginosa F722 was amounted to 1.66 g/l. Biosurfactant production increased twofold because the composition of a modified C-medium was investigated efficiently. $NE_4$Cl or $NaNO_2$ inorganic nitrogens and yeast extract or trypton organic nitrogens were effective, but others inorganic nitrogens and organic nitrogens tested were not efficient far biosurfactant production by P. aeruginosa F722. The optimum concentration of $NH_4$Cl; inorganic nitrogen and yeast extract; organic nitrogen were 0.05% and 0.1%, respectively. In various carbon sources, others with the exception of hydrophobic property substrate (n-alkane) and hydrophilic property substrate (glucose, glycol) were not found to be effective fur biosurfactant production, and 3.0% was better in yield than other concentration of glucose. This yielded C-to-N ratios between 17 and 20. In our experiment, the highest biosurfactant production by P. aeruginosa F722 were observed in 5 days cultivation, containing glucose 3.0%, $NH_4$Cl 0.05%, and yeast extract 0.1% and C-to-N ratio was 20. Optimal pH and temperature for biosurfactant production were 7.0 and $35^{\circ}C$, respectively. Under the optimal culture conditions with glucose, biosurfactant production was amounted to 1.66 g/l. Velocity of biosurfactant production and strain growth increased after nitrogen depletion. The average surface tension of 30 mN/m after the 3 days of incubation under optimal culture condition was measured by ring tensionmeter.

• Bulletin of the Korean Chemical Society
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• v.28 no.12
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• pp.2449-2453
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• 2007

L-[18F]FMAU ([18F]1b) was prepared from the precursor 2-O-[(trifluoromethyl)-sulfonyl]-1,3,5-tri-Obenzoyl- α-L-ribofuranose, by coupling the radioactive fluoro-sugar with the corresponding silylated thymine in 4 steps. The final products, including the α and β anomers, were purified using reverse phase HPLC with an appropriate solvent (5% CH3CN/H2O) at a flow rate of 3.0 mL/min. The total elapsed time of synthesis was about 180-200 min from EOB. The α/β anomeric ratio of the compounds was about 1:9, and the radiochemical purity of the product (β-form) was >98% with decay-corrected yields of 25-35%. All radioactive samples were confirmed using co-injection with pure non-radioactive analogues in every step. In the cellular uptake in vitro test of herpes simplex virus-thymidine kinase (HSV1-TK) gene expressed cells, the percent uptake of injected dose (%ID) of L- and D-FMAU was 37.28 and 65.86, respectively after 240 min incubation. However, the relative uptake (MCA-TK/MCA cellular uptake ratio) of L-FMAU was higher than that of D-FMAU (%ID of L-FMAU, 0.36 and D-FMAU, 0.93 after 240 min incubation in MCA cells). This means that L-FMAU will show better specific HSV1-TK gene expressed cell uptake for selective HSV1-TK gene imaging.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.1
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• pp.90-96
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• 2008

We compared the effects of supplementing $L-lysine{\cdot}SO_4$ to L-lysine HCl on growth performance, nutrient digestibility and nitrogen retention in weaning pigs. A total of 96 crossbred pigs, weaned at $21{\pm}3$ days of age and with an average initial body weight (BW) $6.23{\pm}0.01kg$, were given one of 4 treatments, which translated into 6 replicates of 4 pigs in each pen. The animals were randomly assigned to four dietary treatments according to a randomized completely block design (RCBD) as follows: 1) control-no synthetic lysine, lysine deficient (0.80% total lysine); 2) L-C (= 0.2% L-lysine HCl); 3) K-L-S (= 0.332% $L-lysine{\cdot}SO_4$, A company); 4) C-L-S (= 0.332% $L-lysine{\cdot}SO_4$, B company). Diets were formulated with corn, soy bean meal, and corn gluten meal as the major ingredients, and all nutrients except the lysine met or exceeded NRC requirements (1998). The lysine content of supplemented synthetic lysine was the same in all treatment groups except the control. No clinical health problems associated with the dietary treatments were observed. During the entire experimental period, body weight, average daily gain (ADG) and feed efficiency (G:F ratio) increased (p<0.01) in pigs fed the experimental diets supplemented with L-lysine??HCl or $L-lysine{\cdot}SO_4$ produced by A company, irrespective of the two synthetic lysine sources. Although the supplementation of $L-lysine{\cdot}SO_4$ produced by B company tended to improve the ADG and G:F ratio, significant differences were not seen among all treatments and tended to be lower than the L-C (L-lysine HCl) and K-L-S ($L-lysine{\cdot}SO_4$ groups using the product from A company). The digestibility of crude protein (CP) was increased by the supplementation of synthetic lysine (p<0.05), irrespective of the L-lysine source (L-C, K-L-S, C-L-S). The results of this study showed that ADG, G:F ratio, and CP digestibility improved when $L-lysine{\cdot}SO_4$ or L-lysine HCl was supplemented into the weaning pigs' diet. There was a clear difference in efficacy between the two $lysine{\cdot}SO_4$ products based upon the growth performance of weaning pigs. Consequently, the bioavailability of $lysine{\cdot}SO_4$ products should be evaluated before supplementation of synthetic lysine in swine diets.

• Structural Engineering and Mechanics
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• v.64 no.5
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• pp.621-640
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• 2017

This paper reports the numerical investigation conducted to study the influence of Local-Distortional (L-D) interaction mode buckling on post buckling strength erosion in fixed ended lipped channel cold formed steel columns. This investigation comprises of 81 column sections with various geometries and yield stresses that are carefully chosen to cover wide range of strength related parametric ratios like (i) distortional to local critical buckling stress ratio ($0.91{\leq}F_{CRD}/F_{CRL}{\leq}4.05$) (ii) non dimensional local slenderness ratio ($0.88{\leq}{\lambda}_L{\leq}3.54$) (iii) non-dimensional distortional slenderness ratio ($0.68{\leq}{\lambda}_D{\leq}3.23$) and (iv) yield to non-critical buckling stress ratio (0.45 to 10.4). The numerical investigation is carried out by conducting linear and non-linear shell finite element analysis (SFEA) using ABAQUS software. The non-linear SFEA includes both geometry and material non-linearity. The numerical results obtained are deeply analysed to understand the post buckling mechanics, failure modes and ultimate strength that are influenced by L-D interaction with respect to strength related parametric ratios. The ultimate strength data obtained from numerical analysis are compared with (i) the experimental tests data concerning L-D interaction mode buckling reported by other researchers (ii) column strength predicted by Direct Strength Method (DSM) column strength curves for local and distortional buckling specified in AISI S-100 (iii) strength predicted by available DSM based approaches that includes L-D interaction mode failure. The role of flange width to web depth ratio on post buckling strength erosion is reported. Then the paper concludes with merits and limitations of codified DSM and available DSM based approaches on accurate failure strength prediction.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.1
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• pp.1-9
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• 2013

Long term hydrogen production was investigated in an anaerobic sequencing batch reactor (ASBR) using mixed microflora. Glucose (about 8,250 mg/L) was used as a substrate for the ASBR operation under the condition of pH 5.5 and $37^{\circ}C$ with mixing at 150 rpm. The experiment was carried out over a period of 160 days. Hydrogen yield was 0.8mol $H_2/mol$ glucose with F/M ratio 2 at initial operation period. The hydrogen yield reached to maximum 2.6 mol $H_2/mol$ glucose at 80th day operation. However decreased hydrogen yield was observed after 80 days operation and eventually no hydrogen yield. Although well-known hydrogen producer Clostridium sp. was detected in the reactor by PCR-DGGE analysis, changed reactor operation was the major reason of the decreased hydrogen production, such as low F/M ratio of 0.5 and high propionic acid concentration 2,130 mg/L. Consequently the long period operation resulted in MLSS accumulation and then low F/M ration stimulating propionic acid formation which consumes hydrogen produced in the reactor.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.8 no.1
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• pp.120-139
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• 1996

Experiment was performed to study the heat transfer characteristics in 27 kinds of 15 : 1 scale models of multi-louverred fin heat exchangers with a wide range of variables(R $e_{Lp}$ =100~1, 800, $L_p$/F$p$=0.3~0.9, $\theta$=20$^{\circ}$~40$^{\circ}$). Thermofoil heaters were used to heat the louver fins and the local average Nusselt number for each louver in the louver array was obtained at constant wall temperature conditions. Correlations are developed to predict the heat transfer characteristics and drag coefficients. Generally, the heat transfer characteristics in the multi-louvered fins is shown to be similar to those of the laminar heat transfer on a flat plate. As the Reynolds number, the louver pitch to fin pitch ratio$L_p$/F$p$and the louver angle($\theta$) increase respectively, the average Nusselt number increases, but the variation of average Nusselt number as a function of the louver angle is smaller than that as a function of the louver pitch to fin pitch ratio. In case of$L_p$/F$p$ <0.5, the average Nusselt number of the 3rd louver is especially lower than the others, it is expected that it is due to the flow structure such as a recirculation flow and a flow separation.

• Korean Journal of Ecology and Environment
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• v.41 no.1
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• pp.90-97
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• 2008

The aim of this research was to detect and observe delivering generation effects from $F_0$ generation exposed to 4-nonylphenol 4NP) to $F_1$ generation on Tigriopus japonicus. The nauplius survival rate of $F_1$ from $F_0$ exposed to low 4NP concentration was significantly lower than other concentrations. Among the developmental process, copepodite first emergence day (CE) and adult male first emergence day (ME) on $F_1$ were accelerated relatively high concentration conditions. The sex ratio of $F_1$ was different between control group and treatment groups for 1, 10 and $30{\mu}g\;L^{-1}$ 4NP. The fecundity of $F_1$ was not significantly affected (except, $0.1{\mu}g\;L^{-1}$ 4NP). When 4NP concentrations were increased, first brooding day (FB) of F 1 was generally delayed. The length, width, and biomass of $F_1$ adult female were smaller than control group. In addition, the width and biomass of adult male were narrower or smaller than control group.

• Journal of Welding and Joining
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• v.14 no.5
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• pp.134-144
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• 1996

A study was performed to investigate the properties of base metal and weldment for two HSLA steels and one HY-100 steel. Tensile, yield strength and elongation of HSLA-A steel were superior to those of HY-100 steel and yield ratios in HSLA-A and HSLA-B steels were higher than HY-100 steel owing to the precipitation of $\varepsilon$-Cu phase. The impact energy of HSLA-A steel was greater at all aging temperatures than that of HY-100 steel. HSLA-A and HY-100 steels had low impact transition temperature of about -l$25^{\circ}C$ and high upper shelf energy, The peak hardness of weldment in HSLA-A, HSLA-B and HY-100 steels were Hv 299, Hv 275 and Hv 441, respectively. The hardenability of HY-100 steel was largest due to the higher amount of carbon. The y-groove test showed that HSLA steels had superior resistance to cold cracking. Toughness of weld joint at the F. L. and F. L. ＋1mm in HSLA-A was almost the same as HY-100, but those at F. L.＋3mm and F. L.＋5mm was greater in HSLA-A steel.

• Journal of KSNVE
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• v.9 no.5
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• pp.975-983
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• 1999

This paper describes the dynamic characteristics of the joint structures in case of using the simplified beam model in the F. E. analysis. The modeling errors, when replace the shell with the beam, are investigated through F. E. normal modes analysis. Normal mode analysis were performed to obtain the natural frequencies of the L and T shaped joints with various type of channels. The results were analyzed to access the effects of the models on the accuracy of F.E. analysis by identifying the geometric factors which cause the error. The geometric factors considered are joint angle, channel length, thickness and area ratio of the hollow section to the filled one. The joint stiffness evaluation technique is developed in this study using normal modes analysis with Lumped Mass. With this method, the progressively improved results of F. E. analysis are obtained using the simplified beam model. The static and normal modes analysis are performed with the joint stiffness values obtained by the Kazunori Shimonkakis' virtual stiffness method and the proposed method and these simplified modeling errors are compared.

• International Journal of Naval Architecture and Ocean Engineering
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• v.3 no.2
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• pp.150-158
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• 2011

Present study aims to investigate the influence of relative breakwater width W/L (W=width of breakwater, L=wavelength), wave steepness $H_i/gT^2$ (Hi=incident wave height, T=wave period) and relative wave height d/W (d=water depth) on forces in the moorings of horizontal interlaced multi-layered moored floating pipe breakwater (HIMMFPB) model. Studies were conducted on scaled down physical models having three layers of Poly Vinyl Chloride (PVC) pipes, wave steepness $H_i/gT^2$ varying from 0.063 to 0.849, relative width W/L varying from 0.4 to 2.65 and relative spacing S/D=2 (S=horizontal centre-to-centre spacing of pipes, D=diameter of pipes). Peak mooring forces were also measured and data collected is analyzed by plotting non-dimensional graphs depicting variation of $f_s/{\gamma}W^2$ ($f_s$=Sea side Mooring force, ${\gamma}$=specific weight of water) & $f_l/{\gamma}W^2$ ($f_l$=Lee side Mooring force) with $H_i/gT^2$ for d/W varying from 0.082 to 0.276 and also variation of $f_s/{\gamma}W^2$ and $f_l/{\gamma}W^2$ with W/L for $H_i$/d varying from 0.06 to 0.400.