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http://dx.doi.org/10.4491/KSEE.2013.35.1.001

Long Term Operation of Biological Hydrogen Production in Anaerobic Sequencing Batch Reactor (ASBR)  

Jeong, Seong-Jin (Department of Environmental Engineering, Changwon National University)
Seo, Gyu-Tae (Department of Environmental Engineering, Changwon National University)
Lee, Taek-Soon (Department of Environmental Engineering, Changwon National University)
Publication Information
Journal of Korean Society of Environmental Engineers / v.35, no.1, 2013 , pp. 1-9 More about this Journal
Abstract
Long term hydrogen production was investigated in an anaerobic sequencing batch reactor (ASBR) using mixed microflora. Glucose (about 8,250 mg/L) was used as a substrate for the ASBR operation under the condition of pH 5.5 and $37^{\circ}C$ with mixing at 150 rpm. The experiment was carried out over a period of 160 days. Hydrogen yield was 0.8mol $H_2/mol$ glucose with F/M ratio 2 at initial operation period. The hydrogen yield reached to maximum 2.6 mol $H_2/mol$ glucose at 80th day operation. However decreased hydrogen yield was observed after 80 days operation and eventually no hydrogen yield. Although well-known hydrogen producer Clostridium sp. was detected in the reactor by PCR-DGGE analysis, changed reactor operation was the major reason of the decreased hydrogen production, such as low F/M ratio of 0.5 and high propionic acid concentration 2,130 mg/L. Consequently the long period operation resulted in MLSS accumulation and then low F/M ration stimulating propionic acid formation which consumes hydrogen produced in the reactor.
Keywords
Biological Hydrogen Production; Glucose; Propionic Acid; Anaerobic Sequencing Batch Reactor (ASBR); F/M Ratio;
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