• 한국식품과학회지
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• 제30권3호
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• pp.581-589
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• 1998

$5{\sim}10\;kGy$ 범위의 감마선 조사는 한방약재에 오염된 유기체를 완전사멸 시켜 저장중 미생물학적 품질 안전성을 가져왔으며, 한방약재의 유효성분 추출율을 약 $5{\sim}25%$ 증대시킬 뿐만 아니라 추출시간의 단축효과도 나타냈다. 또한 살균 및 유효성분의 추출율이 증대된 조사선량에서 한방약재의 몇 가지 생리적 활성(항산화력, 항보체능력, 아질산염 소거능, 전자공여능)은 비조사군과 차이를 보이지 않았다.

• 대한한의학방제학회지
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• 제19권1호
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• pp.175-182
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• 2011

Objectives : This study was performed to compare the differences between decoctions extracted by different extraction method and extraction time. Methods : Decoctions were prepared with pressed or non-pressed extraction conditions for 60, 120 and 180min. The yields of extracts, sugar contents, hydrogen ion concentrations(pH), the contents of reference compounds in Yukmijihwang-tang(Liuweidihuang-tang) were investigated. Results : The yields and the dissolved solid content containing sugar content of decoctions were more in pressed extraction method than unpressed extraction method, and they tended to be increased as extraction time increased. The pH values of decoctions methods did not show significant differences between pressed and unpressed extraction methods or extraction times. Most of reference compounds showed higher contents in pressed extraction method than unpressed extraction method and their contents were augmented according to increase of extraction time. The content of paeonol was decreased when extracted in more than 120min with pressed extraction method and tended to be decreased as extraction time increased in unpressed extraction method. Conclusions : The pressed extraction with long extraction time could be useful for decoction of Yukmijihwang-tang(Liuweidihuang-tang). However, another ingredients possible to decrease in such condition need to be considered to determine suitable extraction condition.

• Applied Biological Chemistry
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• 제33권1호
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• pp.8-13
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• 1990

우리나라에서 생산되는 장려품종 중 팔달, 단엽, 장백, 백운, 장엽콩과 재래종 중 검정콩, 갈색아주까리콩 등 7품종을 대상으로 형태적 특성과 표피의 색, 고형분 및 단백질의 추출 수율, 콩의 색소용출속도 등을 조사하였다. 그 결과 콩 개체의 무게는 갈색아주까리콩이 가장 높고 단엽, 장백이 가장 낮은 값을 보였다. 표피의 색에서는 'L'값은 장백이 가장 높았으며 팔달, 백운, 장엽, 단엽 등도 비교적 높은 'L'값을 나타내었다. 'a', 'b'값에서는 장엽이 높았고 검정콩과 갈색아주까리콩이 비교적 낮은 'b'값을 보였다. 콩을 마쇄하여 물로 추출할 때 고형분과 단백질의 수율은 3회까지 대부분이 추출되었으며 이때 이들의 수율은 각각 73.2 %, 83.2 %이었다. 콩 표피의 색소의 용출은 추출온도와 시간에 따라 큰 영향을 받았다. 평형에 도달한 흡광도(A)와 추출온도(T)와의 관계는 A=aT+b의 관계로 표시할 수 있었으며, 색소용출 속도와 온도와의 관계는 $60^{\circ}C$를 중심으로 두개의 직선관계가 있었다. 이 관계에서 계산된 활성화에너지는 고온보다 저온이 더 높았고, Local 1과 Local 2가 가장 높은 활성화에너지 값을 보여 주었다.

• 한국식품과학회지
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• 제24권4호
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• pp.382-386
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• 1992

열수출시 미역 추출액의 품질 특성에 미치는 온도의 영향을 조사하였다. 추출액의 고형분, 단백질의 농도와 수율은 초기추출시간 동안 추출 온도 $50^{\circ}C$에서 $100^{\circ}C$로 증가할수록 높아졌다. 그러나 상징액율은 $50^{\circ}C$와 $100^{\circ}C$에서 가장 낮은 값을 보였으며 $100^{\circ}C$에서 1시간 추출할 때 고형분과 단백질 수율은 각각 21.56%와 4.76%였다. 점도는 $100^{\circ}C$에서 2시간 추출할 때 가장 높았으며 다른 온도의 추출액 점도에 비해 $2.5{\sim}4.0$배 높았다. 탁도는 추출 1시간 이후 차츰 감소하였으며 고형분과 단백질의 초기 추출속도에서 계산된 활성화에너지는 1.18과 3.9 Cal/mole이었다.

• Journal of the Korean Wood Science and Technology
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• 제42권2호
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• pp.172-182
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• 2014

Extraction is a necessary element in the bioconversion of lignocellulosics to fuels and chemicals. Although various forms of chemical pretreatment of cellulosic materials have been proposed, their effectiveness varies depending on the treatment conditions and substrate. In this study, mixed hardwood (MH) and loblolly pine (LP) were pretreated with dilute acid in a 100 mL accelerated solvent extraction (ASE) at the predetermined optimal conditions: temperature: $170^{\circ}C$, acid concentration: 0.5% (w/v), and reaction time: 2~64 min. This method was highly effective for extracting the hemicellulose fraction. Total xmg (defined as the sum of xylose, mannose, and galactose) can be extracted from milled MH and LP through pressurized dilute acid treatment in maximum yields of 12.6 g/L and 15.3 g/L, respectively, representing 60.5% and 70.4% of the maximum possible yields, respectively. The crystallinity index increased upon pretreatment, reflecting the removal of the amorphous portion of biomass. The crystalline structure of the cellulose in the biomass, however, was not changed by the ASE extraction process.

• Preventive Nutrition and Food Science
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• 제19권2호
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• pp.124-127
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• 2014

The effect of feed moisture contents (30%, 40%, and 50%) and screw speed (200 rpm, 250 rpm, and 300 rpm) on the corn fiber gum (CFG) yield and soluble arabinoxylans (SAX) content of destarched corn fiber was investigated. The CFG yields and SAX contents of extruded, destarched corn fiber were higher than that of destarched corn fiber. In extruded, destarched corn fiber, increased screw speed and decreased feed moisture contents resulted in a higher SAX contents. The maximum yields of CFG obtained from extruded, destarched corn fiber were $79.1{\pm}19.0g/kg$ (30% feed moisture content) and $82.3{\pm}11.30g/kg$ (300 rpm screw speed). The highest SAX content was also observed at a screw speed of 300 rpm. The results of the present study show that water extraction and extrusion combined have the potential to increase CFG and SAX yields from corn fiber.

• Journal of the Korean Wood Science and Technology
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• 제25권1호
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• pp.23-27
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• 1997

Alkali-soluble extracts were prepared from barks of Japanese larch(Larix leptolepis). Siberian larch(Larix gmelineii) and Radiata pine(Pinus radiata) The effect of various factors, such as particle size, liquor ratio, extraction temperature, and reaction time, on the extracts yield was investigated. Particle sizes affected the alkali-soluble extracts; the finer the particle size, the higher extracts and extract efficiency. High temperature and high liquor ratio were more effective. In the range of 0.25% to 1% NaOH concentration, alkali extracts were increased with the increasing alkali concentration. However, extract yields were leveled off above 0.5% alkali concentration. Extractions with NaHCO3 were almost equivalent to those of NaOH extraction. 1% NaOH and 1% NaHCO3 resulted in the highest yields of alkaline extracts. Extracts from Japanese larch were lower than those from Siberian larch and Radiata pine barks. Siberian larch bark was the best raw material for tannin adhesives, because its extractive yield was higher than those of the other barks. Concerning pH of alkaline media during extraction, small increases of the extract yields were resulted at range of pH 7 to pH 9, while a large increases from pH 9.0 to pH 12. This phenomenon is attributed to higher alkalinity.

• 대한약침학회지
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• 제21권2호
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• pp.48-60
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• 2018

Objective: The aim of this study is to optimize the extraction of beneficial substance from Cudrania tricuspidata leaves grown at Jirisan Mountain in South Korea by three different solvents depending on extraction time and at different temperature. Methods: The total phenolic contents were determined by the method reported by $S{\acute{a}}nchez$-Moreno et al. The total flavonoid contents were analyzed by Slinkard and Singleton. The DPPH radical scavenging activity was determined according to the method reported by Blois Results: The extraction yield for each solvent is 9.05-14.1%, 2.17-5.67%, and 2.3-3.9% for D.W., ethanol, and hexane, respectively. The overall results were maximized for the extract obtained with D.W. for 5 min at $100^{\circ}C$. The average phenol contents were 77.11, 45.64, and 0.343 mg/g at $100^{\circ}C$ in water, $78^{\circ}C$ in ethanol, and $68^{\circ}C$ in hexane, respectively. The flavonoid contents were the highest in the materials extracted with D.W., and were increased with increasing temperature, regardless of the extraction solvents, whether water (green), polar organic ethanol, or nonpolar organic hexane. In the ethanol extract, the flavonoid contents are increased gradually from 5.66 mg/g to 7.73 mg/g. The total flavonoid contents were proportional to the concentrations of the water extracts, ranging from 4.14 mg/g to 48.89 mg/g. The antioxidative activities of the water-extracted compounds are generally increased with increasing temperature from 42.5% to 85.5%. Those of the hexane extracts are increased slowly from 3.79% to 8.8%, while those of ethanol extracts are increased from 29.8% to 47.4%. Conclusion: The extraction yields were dependent upon solvents for extraction as well as extraction time and the temperature. The optimal extraction time was 5 min and the extraction yields were increased with increasing temperature excepted hexane. Of the three tested extraction solvents, the greenest solvent of water shows excellent results, suggesting that water is among the most effective solvents for natural sample extractions for general medicinal, pharmaceutical, and food applications.

• 대한화학회지
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• 제10권1호
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• pp.1-3
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• 1966

The organic yields (i.e. fraction of nuclear events resulting in organic compound formation) of the radioative neutron capture reactions of halogens in purified bromobenzene have been determined varying extraction time, at $100^{\circ}C$ for thermal effect, varying irradiation time, varying neutron flux and with additional U. V. irradiation. Among the important results are; (1) The organic yields show no remarkable fluctuations with time following neutron irradiation; (2) The organic yields show no change with thermal energy; (3) The organic yields of degassed samples are same in different length of irradiation time whereas the yields of the samples in open air appear to increase with increasing time of irradiation (4) The organic yields increase remarkably with increased neutron flux; (5) The organic yields show a sharp increase by additional U. V. irradiation after neutron irradiation.

• Journal of Ginseng Research
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• 제40권4호
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• pp.415-422
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• 2016

Background: Rare ginsenosides in Panax quinquefolius L. have strong bioactivities. The fact that it is hard to obtain large amounts of rare ginsenosides seriously restricts further research on these compounds. An easy, fast, and efficient method to obtain different kinds of rare ginsenosides simultaneously and to quantify each one precisely is urgently needed. Methods: Microwave-assisted extraction (MAE) was used to extract nine kinds of rare ginsenosides from P. quinquefolius L. In this article, rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, 20(S)-Rg3, 20(R)-Rg3, Rk1, and Rg5] were identified by high performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry. The quantity information of rare ginsenosides was analyzed by HPLC-UV at 203 nm. Results: The optimal conditions for MAE were using water as solvent with the material ratio of 1:40 (w/v) at a temperature of $145^{\circ}C$, and extracting for 15 min under microwave power of 1,600 W. Seven kinds of rare ginsenosides [20(S)-Rh1, 20(R)-Rh1, Rg6, F4, Rk3, Rk1, and Rg5] had high extraction yields, but those of 20(S)-Rg3 and 20(R)-Rg3 were lower. Compared with the conventional method, the extraction yields of the nine rare ginsenosides were significantly increased. Conclusion: The results indicate that rare ginsenosides can be extracted effectively by MAE from P. quinquefolius L. in a short time. Microwave radiation plays an important role in MAE. The probable generation process of rare ginsenosides is also discussed in the article. It will be meaningful for further investigation or application of rare ginsenosides.