• 제목/요약/키워드: extraction techniques

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Optical Hough Transform을 사용한 피쳐 추출 기법 (Feature Extraction Techniques Using Optical Hough Transform)

  • 진성일
    • 한국광학회:학술대회논문집
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    • 한국광학회 1990년도 제5회 파동 및 레이저 학술발표회 5th Conference on Waves and lasers 논문집 - 한국광학회
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    • pp.121-125
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    • 1990
  • Optical Hough transform technique is introduced to obtain the straight line features in parallel from the input scene images. Experimental results are also provided to demonstrate the advantage of such optical parallel processor over the digital one. Peaks in optical Hough space are free from quantization noise and thus easy to detect.

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Improved DNA Extraction Method for Molecular Diagnosis from Smaller numbers of Cells

  • Oh, Seo Young;Han, Jeong Yeon;Lee, So Ra;Lee, Hoon Taek
    • 대한임상검사과학회지
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    • 제46권3호
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    • pp.99-105
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    • 2014
  • Isolating total DNA from small samples using traditional methods is difficult and inefficient mainly due to loss of DNA during filtration and precipitation. With advances in molecular pathology, DNA extraction from micro-dissected cells has become essential in handling clinical samples. Genomic DNA extraction using small numbers of cells can be very important to successfully PCR amplify DNA from small biopsy specimens. We compared our experimental genomic DNA extraction method (A) with two other commercially available methods: using spin columns (B), and conventional resins (C), and determined the efficacy of DNA extraction from small numbers of cells smeared on a glass slide. Approximately 50, 100, 200, 500 and 1000 cells were isolated from fine needle aspiration biopsy (FNAB) slides aspirated from histologically proven papillary thyroid carcinoma masses. DNA was extracted using the three techniques. After measuring DNA quantity, PCR amplification was performed to detect the ${\beta}$-globin and $BRAF^{V600E}$ gene mutations. DNA extracted by method (A) showed better yield than the other methods in all cell groups. With our method, a suitable amount of genomic DNA to produce amplification was extracted from as few as 50 cells, while more than 100 to 200 cells were required when methods (B) or (C) were applied. Our genomic DNA extraction method provides high quality and improved yields for molecular analysis. It will be especially useful for paucicellular clinical samples which molecular pathologists often confront when handling fine needle aspiration cytology, exfoliative cytology and small biopsy specimens.

Reduction in post extraction waiting period for dental implant patients using plasma rich in growth factors: an in vivo study using cone-beam computed tomography

  • Arya, Varun;Malhotra, Vijay Laxmy;Rao, JK Dayashankara;Kirti, Shruti;Malhotra, Siddharth;Sharma, Radhey Shyam
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • 제45권5호
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    • pp.285-293
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    • 2019
  • Objectives: This study examined the effects of plasma-rich growth factors (PRGF) on accelerating bone regeneration/repair in fresh extraction sockets, and determined the quality and quantity of bone by assessing the bone density using cone-beam computed tomography (CBCT). Materials and Methods: Twenty patients, who had undergone bilateral extractions, were included in this study. In one extraction socket, PRGF was used and covered with an autologous fibrin plug. Nothing was used in the opposite side extraction socket. Thirteen weeks post extraction, the level of bone regeneration was evaluated on both sides with CBCT. Results: At the end of the study, the mean bone density according to the Hounsfield units (HU) in the control group and PRGF group was 500.05 HU (type III bone type) and 647.95 HU (type II bone type), respectively. Conclusion: This study recommends the use of PRGF in post extraction sites to accelerate the rate of bone regeneration and improve the quality of regenerated bone. The technique to process PRGF was simple compared to previously mentioned techniques used for platelet-rich plasma (PRP) preparation. PRP preparation requires a two-cycle centrifugation procedure, leading to a longer processing time.

Handspace Solid Phase Microextraction 방법에 의한 HANs 분석에 관한 연구 (Analysis of Haloacetonitriles in Drinking Water Using Headspace-SPME Technique with GC-MS)

  • 조덕희
    • 상하수도학회지
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    • 제18권5호
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    • pp.628-637
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    • 2004
  • In many drinking water treatment plants, chlorination process is one of the main techniques used for the disinfection of water. This disinfecting treatment leads to the formation of disinfection by-products (DBPs) such as haloacetonitriles (HANs), trihalomethanes (THMs), haloacetic acids (HAAs). In this study, headspace-solid phase microextraction (HS- SPME) technique was applied for the analysis of HANs in drinking water. The effects of experimental parameters such as selection of SPME fiber, the addition of salts, magnetic stirring, extraction temperature, extraction time and desorption time on the analysis were investigated. Analytical parameters such as linearity, repeatability and detection limits were also evaluated. The $50/30{\mu}m$-divinylbenzene/carboxen/polydimethylsiloxane fiber, extraction time of 30 minutes, extraction temperature of $20^{\circ}C$ and desorption time of 1 minute at $260^{\circ}C$ were the optimal experimental conditions for the analysis of HANs. The correlation coefficients ($r^2$) for HANs was 0.9979~0.9991, respectively. The relative standard deviations (%RSD) for HANs was 2.3~7.6%, respectively. Detection limits (LDs) for HANs was $0.01{\sim}0.5{\mu}g/L$, respectively.

초고압 시스템을 이용한 생물 산업의 적용 (Application of Biological industry using High Hydrostatic Pressure (HHP) system)

  • 이광진;최선도
    • KSBB Journal
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    • 제23권5호
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    • pp.362-368
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    • 2008
  • 본고에서는 초고압 시스템 (High hydrostatic pressure (HHP))의 특성, 종류, 응용을 예로 들어 설명하였다. 초고압 기술은 식품의 살균, 식품 보존기간연장, 유효성분의 추출 등에 선택적으로 적합하게 적용하여 생물산업공정에 효율적으로 응용 할 수 있다. 초고압 시스템을 효율적으로 적용하기 위한 최적조건을 선정하는 것은 많은 조업 변수에 대한 고려를 해야 하는 힘든 작업이다. 따라서 하드웨어에 대한 기초 실험과 예비 생산을 통하여 공정에 대한 모사를 검증하고 생산 효율을 증가시킬 수 있다. 또한 초고압 시스템의 중요성이 증가함에 따라 고성능 및 사용이 더욱 편리한 시스템이 나타날 것이며, 많은 영역의 바이오산업에서 필수적으로 이용될 것이다.

Multiple Active Appearance Model을 이용한 얼굴 특징 추출 기법 (Facial Feature Extraction using Multiple Active Appearance Model)

  • 박현준;김광백;차의영
    • 한국전자통신학회논문지
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    • 제8권8호
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    • pp.1201-1206
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    • 2013
  • 영상에서 얼굴 및 얼굴 특징을 추출하기 위한 기법으로 active appearance model(AAM)이 있다. 본 논문에서는 두 개의 AAM을 이용하여 얼굴 특징을 추출하는 multiple active appearance model(MAAM) 기법을 제안한다. 두 개의 AAM은 학습 데이터에 대한 파라미터를 조절하여 상반되는 장단점을 가지도록 생성하고, 서로의 단점을 보완할 수 있도록 한다. 제안된 방법의 성능을 평가하기 위해 100장의 영상에 대해서 얼굴 특징추출 실험을 하였다. 실험 결과 기존의 AAM 하나만을 사용하는 기법에 비해 적은 횟수의 피팅만으로도 정확도 높은 결과를 얻을 수 있었다.

Business Model Mining: Analyzing a Firm's Business Model with Text Mining of Annual Report

  • Lee, Jihwan;Hong, Yoo S.
    • Industrial Engineering and Management Systems
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    • 제13권4호
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    • pp.432-441
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    • 2014
  • As the business model is receiving considerable attention these days, the ability to collect business model related information has become essential requirement for a company. The annual report is one of the most important external documents which contain crucial information about the company's business model. By investigating business descriptions and their future strategies within the annual report, we can easily analyze a company's business model. However, given the sheer volume of the data, which is usually over a hundred pages, it is not practical to depend only on manual extraction. The purpose of this study is to complement the manual extraction process by using text mining techniques. In this study, the text mining technique is applied in business model concept extraction and business model evolution analysis. By concept, we mean the overview of a company's business model within a specific year, and, by evolution, we mean temporal changes in the business model concept over time. The efficiency and effectiveness of our methodology is illustrated by a case example of three companies in the US video rental industry.

3D BUILDING INFORMATION EXTRACTION FROM A SINGLE QUICKBIRD IMAGE

  • Kim, Hye-Jin;Han, Dong-Yeob;Kim, Yong-Il
    • 대한원격탐사학회:학술대회논문집
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    • 대한원격탐사학회 2006년도 Proceedings of ISRS 2006 PORSEC Volume I
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    • pp.409-412
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    • 2006
  • Today's commercial high resolution satellite imagery such as IKONOS and QuickBird, offers the potential to extract useful spatial information for geographical database construction and GIS applications. Recognizing this potential use of high resolution satellite imagery, KARI is performing a project for developing Korea multipurpose satellite 3(KOMPSAT-3). Therefore, it is necessary to develop techniques for various GIS applications of KOMPSAT-3, using similar high resolution satellite imagery. As fundamental studies for this purpose, we focused on the extraction of 3D spatial information and the update of existing GIS data from QuickBird imagery. This paper examines the scheme for rectification of high resolution image, and suggests the convenient semi-automatic algorithm for extraction of 3D building information from a single image. The algorithm is based on triangular vector structure that consists of a building bottom point, its corresponding roof point and a shadow end point. The proposed method could increase the number of measurable building, and enhance the digitizing accuracy and the computation efficiency.

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Antioxidant and Anti-cancer Cell Proliferation Activity of Propolis Extracts from Two Extraction Methods

  • Khacha-ananda, Supakit;Tragoolpua, Khajornsak;Chantawannakul, Panuwan;Tragoolpua, Yingmanee
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권11호
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    • pp.6991-6995
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    • 2013
  • Antioxidant activity, total phenolic, total flavonoid compounds and cytotoxicity to cancer cell lines of propolis extracts from two extraction methods were investigated in this study. Propolis was collected from Phayao province and extracted with 70% ethanol using maceration and sonication techniques. The antioxidant activity was evaluated by DPPH assay. Total phenolic and flavonoid compounds were also determined. Moreover, the cytotoxicity of propolis was evaluated using MTT assay. The percentage propolis yield after extraction using maceration (18.1%) was higher than using sonication (15.7%). Nevertheless, antioxidant and flavonoid compounds of the sonication propolis extract were significant greater than using maceration. Propolis extract from sonication showed antioxidant activity by $3.30{\pm}0.15$ mg gallic acid equivalents/g extract. Total phenolic compound was $18.3{\pm}3.30$ mg gallic acid equivalents/g extract and flavonoid compound was $20.49{\pm}0.62$ mg quercetin/g extract. Additionally, propolis extracts from two extraction methods demonstrated the inhibitory effect on proliferation of A549 and HeLa cancer cell lines at 24, 48 and 72 hours in a dose-dependent manner. These results are of interest for the selection of the most appropriate method for preparation of propolis extracts as potential antioxidant and anticancer agents.