• Title/Summary/Keyword: extraction condition

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The Biological Activity from Prunella vulgaris Extracts (하고초(Prunella vulgaris) 추출물의 생리활성)

  • Kim, Jin-Sung;Lee, Ju-Yeong;Park, Ki-Tae;An, Bong-Jeun;Lee, Sun-Ho;Cho, Young-Je
    • Food Science and Preservation
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    • v.20 no.2
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    • pp.234-241
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    • 2013
  • The phenolic compounds of water extracts from Prunella vulgaris were highest at 9.25 mg/g, respectively, when various extraction solvents were used. The optimum condition for extracting phenolic compounds from Prunella vulgaris was extraction in water for 18hr. The DPPH-scavenging activities of Prunella vulgaris were highest at the water extracts. The ABTS radical cation decolorization was higher than 40% in the range of 0~100% ethanol extract section. The antioxidant protection factor on the lipophilic phenolic metabolites was shown to be 1.1 PF in the water extracts from Prunella vulgaris. The TBARS was lower than the control ($0.53{\mu}M$) in all the sections. The tyrosinase inhibitory effect, which is related to skin whitening, was above 40%, and for the anti-wrinkle effect, the elastase inhibition activity was above 40% at 0.2 mg/mL. The astringent effect of the Prunella vulgaris 40% ethanol extracts was 98.1% at 1 mg/mL. As a result, it can be concluded that Prunella vulgaris has the potential to be used as a cosmetic material.

Microbiological Hazard Analysis for HACCP System Application to Non Heat-Frozen Carrot Juice (비가열냉동 당근주스의 HACCP 시스템 적용을 위한 미생물학적 위해 분석)

  • Lee, Ung-Soo;Kwon, Sang-Chul
    • Journal of Food Hygiene and Safety
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    • v.29 no.2
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    • pp.79-84
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    • 2014
  • This study has been performed for about 270 days at analyzing biologically hazardous factors in order to develop HACCP system for the non heat-frozen carrot juice. A process chart was prepared by manufacturing process of raw agricultural products of non heat-frozen carrot juice, which was contained water and packing material, storage, washing, cutting, extraction of the juice, internal packing, metal detection, external packing, storage and consignment (delivery). As a result of measuring Coliform group, Staphylococcus aureus, Salmonella spp., Bacillus cereus, Listeria Monocytogenes, Enterohemorrhagic E. coli before and after washing raw carrot, Standard plate count was $4.7{\times}10^4CFU/g$ before washing but it was $1.2{\times}10^2CFU/g$ detected after washing. As a result of testing airborne bacteria (Standard plate count, Coliform group, Yeast and Fungal) depending on each workplace, number of microorganism of in packaging room, shower room and juice extraction room was detected to be 10 CFU/Plate, 60 CFU/Plate, 20 CFU/Plate, respectively. As a result of testing palm condition of workers, as number of Standard plate count, Coliform group and Staphylococcus aureus was represented to be high as $6{\times}10^4CFU/cm^2$, $0CFU/cm^2$ and $0CFU/cm^2$, respectively, an education and training for individual sanitation control was considered to be required. As a result of inspecting surface pollution level of manufacturing facility and devices, Coliform group was not detected in all the specimen but Standard plate count was most dominantly detected in scouring kier, scouring kier tray, cooling tank, grinding extractor, storage tank and packaging machine-nozzle as $8.00{\times}10CFU/cm^2$, $3.0{\times}10CFU/cm^2$, $4.3{\times}10^2CFU/cm^2$, $7.5{\times}10^2CFU/cm^2$, $6.0{\times}10CFU/cm^2$, $8.5{\times}10^2CFU/cm^2$ respectively. As a result of analyzing above hazardous factors, processing process of ultraviolet ray sterilizing where pathogenic bacteria may be prevented, reduced or removed is required to be controlled by CCP-B (Biological) and critical level (critical control point) was set at flow speed is 4L/min. Therefore, it is considered that thorough HACCP control plan including control criteria (point) of seasoning fluid processing process, countermeasures in case of its deviation, its verification method, education/training and record control would be required.

Analysis of Cellular Components of Starch-Utilizing Yeast Sporobolomyces holsaticus (전분이용성 효모 Sporobolomyces holsaticus의 균체성분 분석에 대하여)

  • Park, Wan-Soo;Koo, Young-Jo;Shin, Dong-Hwa;Suh, Kee-Bong
    • Korean Journal of Food Science and Technology
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    • v.15 no.2
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    • pp.170-176
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    • 1983
  • Starchy single cell protein produced by a starch-utilizing yeast, Sporobolomyces holsaticus FRI Y-5 was analyzed for its composition such as intracellular protein, amino acids, fatty acids, minerals, vitamins and pigments. It was shown that it contained 33.08% of total carbohydrate, 45.63% of crude protein, 20.01% of crude lipid, 3.24% of ash and 4.46% of pigment. Whole cell extracted by cold and hot NaOH method contained 40.89% of soluble protein and the estimated nucleic acid content from crude and soluble protein contents was about 7.6%. The sulphur-containing amino acids, threonine, isoleucine and valine were analyzed to be the limiting amino acids in the starchy SCP, and the protein score was calculated as 89.4. It was shown from its fatty acid analysis that it contained $6.5%\;of\;C_{16:0}$, $2.4%\;of\;C_{18:0}$, $81.9%\;of\;C_{18:1}$, $3.2%\;of\;C_{18:2}$, and $6.0%\;of\;C_{18:3}$. Also it was observed that it contained, per 100 g of dry cell, 365.33mg of Mg and 282.75mg of K more than Fe and Ca. The content of Vit. $B_2$ was 3.7mg per 100 g of dry cell, but niacin was not detected under this experimental condition. The UV-visible scanning result of pigment extract showed that the yeast contained carotenoid and unknown pigments.

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A Study on the Removal of Aromatic Compounds from Soil and Zeolite Using Supercritical Carbon Dioxide (초임계 이산화탄소를 이용한 토양과 제올라이트중의 방향족 화합물 제거에 관한 연구)

  • Bae, Won;Shin, Bohyun;Kang, Hoseok;Kim, Hwayong
    • Clean Technology
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    • v.9 no.4
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    • pp.197-206
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    • 2003
  • We performed removal of aromatic compounds, benzene and toluene, from soil and zeolite using supercritical carbon dioxide. Extraction was performed at $50^{\circ}C$ and 27.7 MPa with changing the extent of pollutant concentration and the results were compared and analyzed. Experiments were carried out using flow method and high pressure extractor of 1.27 cm in diameter and 25cm in length was used. The pollutants were sampled every ten minutes and their concentrations were analyzed with GC/FID. As a result, highly contaminated sample followed solubility/elution model and slightly contaminated sample followed desorption/kinetics model. At the same condition benzene was extracted faster than toluene. In the case of zeolite, more time is required to extract pollutants than soil. This phenomena was due to high adsorption capacity of zeolite. In the case of highly contaminated soil, we could correlate experimental data using simple Brady's fixed bed extractor model. But in the case of slightly contaminated soil, that was governed with desorption/kinetics model, there was some errors.

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Development of Garlic & Onion Yield Prediction Model on Major Cultivation Regions Considering MODIS NDVI and Meteorological Elements (MODIS NDVI와 기상요인을 고려한 마늘·양파 주산단지 단수예측 모형 개발)

  • Na, Sang-il;Park, Chan-won;So, Kyu-ho;Park, Jae-moon;Lee, Kyung-do
    • Korean Journal of Remote Sensing
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    • v.33 no.5_2
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    • pp.647-659
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    • 2017
  • Garlic and onion are grown in major cultivation regions that depend on the crop condition and the meteorology of the production area. Therefore, when yields are to be predicted, it is reasonable to use a statistical model in which both the crop and the meteorological elements are considered. In this paper, using a multiple linear regression model, we predicted garlic and onion yields in major cultivation regions. We used the MODIS NDVI that reflects the crop conditions, and six meteorological elements for 7 major cultivation regions from 2006 to 2015. The multiple linear regression models were suggested by using stepwise regression in the extraction of independent variables. As a result, the MODIS NDVI in February was chosen the significant independent variable of the garlic and onion yield prediction model. In the case of meteorological elements, the garlic yield prediction model were the mean temperature (March), the rainfall (November, March), the relative humidity (April), and the duration time of sunshine (April, May). Also, the rainfall (November), the duration time of sunshine (January), the relative humidity (April), and the minimum temperature (June) were chosen among the variables as the significant meteorological elements of the onion yield prediction model. MODIS NDVI and meteorological elements in the model explain 84.4%, 75.9% of the garlic and onion with a root mean square error (RMSE) of 42.57 kg/10a, 340.29 kg/10a. These lead to the result that the characteristics of variations in garlic and onion growth according to MODIS NDVI and other meteorological elements were well reflected in the model.

Process Development of Red Ginseng Production by Microwave-assisted Low Temperature Vacuum Dry and Characteristics of Products (마이크로파 저온진공건조 기술을 이용한 홍삼제조공정 개발 및 제품특성에 관한 연구)

  • Lee, Sang-Ho;Ji, Joong-Gu
    • Journal of the Korean Applied Science and Technology
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    • v.34 no.2
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    • pp.305-314
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    • 2017
  • The purpose of this study was to develop the high efficiency of ginseng by using microwave low temperature vacuum drying technology. In red ginseng manufacturing processes, the study results compared the hot-air drying red ginseng dried during 24hours in $60-70^{\circ}C$ and redried during 72hours in $40^{\circ}C$ after the steaming ginseng with the red ginseng dried in microwave low temperature vacuum dryer on condition that 900 watt, 2.45 MHz, 50 mmHg during 5 hours and redried during 2 hours on 750 mmHg after the steaming ginseng about observation of tissue, sensory evaluation and a change of ginsenoside and crude saponin content. As a result, the red ginseng in microwave low temperature vacuum was had high brightness, the surface turned into porosity tissue and added more flavor, decreased bitterness highly on the contrary increased sweetness at the same time that elevated the comprehensive preference. Moreover, In a short time, the content of ginsenosides $Rg_1$ and $Rb_1$ increased about sixfold, eightfold in one time zone but there were no wide difference in content of $Rg_3$ as compared to the hot-air drying red ginseng. Finally, content of crude saponin was increased widely at 10-20 minutes and stayed high crude saponin content consistently. Therefore, this result indicated that the red ginseng in microwave low temperature vacuum increased extraction yields of the ginsenosides and crude saponin through a change of porosity tissue and improved flavor and texture compare with the general hot air dried red ginseng in a short time. According to these results, that provided that could increase the preference about red ginseng.

COMPARATIVE STUDIES OF THE ADHESIVE QUALITIES OF POLYCARBOXYLATE CEMENTS (카복실레이트계 시멘트의 접착력에 관한 비교 연구)

  • Lee, Han-Moo
    • The Journal of Korean Academy of Prosthodontics
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    • v.17 no.1
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    • pp.23-34
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    • 1979
  • In this study, the adhesive strength of three commercial polycarboxylate cements to ten types of dental casting alloys, such as gold, palladium, silver, indium, copper, nickel, chromium, and human enamel and dentine were measured and compared with that of a conventional zinc phosphate cement. The $8.0mm{\times}3.0mm$ cylindrical alloy specimens were made by casting. The enamel specimens were prepared from the labial surface of human upper incisor, and the dentine specimens were prepared from the occulusal surface of the human molar respectively. Sound extracted human teeth, which had been kept in a fresh condition since, extraction, were mounted in a wax box with a cold-curing acrylic resin to expose the flattened area. The mounted teeth were then placed in a Specimen Cutter (Technicut) and were cut down under a water spray, and then the flat area on the all specimens were ground by hand with 400 and 600 grit wet silicone carbide paper. Two such specimens were then cemented together face-to-face with freshly mixed cement, and moderate finger pressure was applied to squeeze the cement to a thin and uniform film. All cemented specimens were then kept in a thermostatic humidor cabinet regulated at $23{\pm}2^{\circ}C.$ and more than 95 per cent relative humidity and tested after 24 hours and 1 week. Link chain was attached to each alloy specimen to reduce the rigidity of the jig assembly, and then all the specimens were mounted in the grips of the Instron Universal Testing Machine, and a tensile load was delivered to the adhering surface at a cross head speed of 0.20 mm/min. The loads to which the specimens were subjected were recorded on a chart moving at 0.50 mm/min. The adhesive strength was determined by measuring the load when the specimen separated from the cement block and by dividing the load by the area. The test was performed in a room at $23{\pm}2^{\circ}C.$ and $50{\pm}10$ per cent relative humidity. A minimum of five specimens were tested each material and those which deviated more than 15 per cent from the mean were discarded and new specimens prepared. From the experiments, the following results were obtained. 1) It was found that the adhesive strength of the polycarboxylate cement to all alloys tested was considerably greater than that of the zinc phosphate cement. 2) The adhesive strength of the polycarboxylate cements was superior to the non precious alloys, such as the copper, indium, nickel and chromium alloys, but it was inferior to the precious gold, silver and palladium alloys. 3) Surface treatment of the alloy was found to be an important factor in achieving adhesion. It appears that a polycarboxylate cement will adhere better to a smooth surface than to a rough one. This contrasts with zinc phosphate cements, where a rough helps mechanical interlocking. 4) The adhesion of the polycarboxylate cement with enamel was found superior to its adhesion with dentine.

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Changes of Antioxidant Activity in Juglans mandshrica Maxim. Leaves by Far Infrared Ray Irradiation (원적외선 조사에 따른 가래나무 잎의 항산화 활성 변화)

  • Eom, Seok-Hyun;Park, Hyoung-Jae;Jin, Cheng-Wu;Park, Seung-Moon;Kim, Myong-Jo;Yu, Chang-Yeon;Cho, Dong-Ha
    • Korean Journal of Medicinal Crop Science
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    • v.15 no.4
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    • pp.266-270
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    • 2007
  • This research was conducted to investigate antioxidant activities of Juglans mandshrica forages by the irradiation of far infrared ray during leaf drying processes before compound extraction, with its potential use for activating antioxidants. The contents of total phenolics and flavonoids in the basis of a gram leaf dry weight were maximized in 20 min at 30 V of far infrared irradiation. Total phenolic content was 25.43 mg/g in the FIR treatment, while it was 21.12 mg/g in the non-FIR treatment control. Total flavonoid content was 39.38 mg/g in the FIR treatment, while it was 32.78 mg/g in the non-FIR treatment control. The contents were decreased when the condition of far infrared ray irradiation was higher voltage and longer treatment time, exhibiting 16.93 mg/g of total phenolics and 23.78 mg/g of total flavonoids in 30 min at 50 V of far infrared irradiation. The antioxidant activities were shown to positive relationships with the contents of total phenolics and total flavonoids. In this study, we concluded that content of bioactive molecules relating antioxidant activity in Juglans mandshrica forages is optimized in 20 min at 30 V of far infrared irradiation for leaf drying process.

Stromelysin-1 and Membrane type-MMP-1 Expressions in Human Chronic Periodontitis with Type 2 Diabetes Mellitus (단순 만성 치주염 환자와 제 2형 당뇨병을 동반한 만성 치주염 환자에서 Stromelysin-1와 Membrane type-MMP-1 Expressions)

  • Ryu, Sang-Ho;Park, Jin-Woo;Suh, Jo-Young;Lee, Jae-Mok
    • Journal of Periodontal and Implant Science
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    • v.38 no.4
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    • pp.629-638
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    • 2008
  • Purpose: The purposes of this study were to compare and quantify the expression of Stromelysin-1 and MT-MMP-1 in the gingival tissues of patients with type 2 diabetes mellitus(DM) and healthy adults with chronic periodontitis. Materials and Methods: Gingival tissue samples were obtained during periodontal surgery or tooth extraction. According to the patient's systemic condition & clinical criteria of gingiva, each gingival sample was devided into three groups. Group 1 (n=8) is clinically healthy gingiva without bleeding and no evidence of bone resorption or periodontal pockets, obtained from systemically healthy 8 patients. Group 2 (n=8) is inflammed gingiva from patients with chronic periodontitis. Group 3 (n=8) is inflammed gingiva from patients with chronic periodontitis associated with type 2 DM. Tissue samples were prepared and analyzed by Western blotting. The quantification of Stromelysin-1 and MT-MMP-1 were performed using a densitometer and statistically analyzed by one-way ANOVA followed by Tukey test. Results: In the analysis of expression levels, Stromelysin-1 and MT-MMP-1 expressions were similar in group 1 and 2. Stromelysin-1 and MT-MMP-1 expressions was more increased in group 3 than group 1, 2. The difference between group 3 and group 1, 2 was statistically significant. Also, in the interrelationship of Stromelysin-1 and MT-MMP-1 expressions, expressions of Stromelysin-1 and MT-MMP-1 showed increasing tendency in chronic periodontitis associated with type 2 DM and it seems that the MT-MMP-1 expressions were increasing in proportion to Stromelysin-1 expressions. Conclusion: It is suggested that Stromelysin-1 and MT-MMP-1 may be partly involved in the progression of periodontal inflammation associated with type 2 DM, as related to a metabolism of other factors, such as AGE, plasmin and other inflammatory mediators. Therefore, the expression levels of Stromelysin-1 and MT-MMP-1 can be inflammatory markers of periodontal inflammed tissue with type 2 DM.

THE EVALUATION OF THE PATIENTS TAKING CBCT IN DEPARTMENT OF PEDIATRIC DENTISTRY (소아치과에서 Cone beam형 전산화단층영상을 이용한 환자의 평가)

  • Jeon, Hye-Jin;Yang, Yeon-Mi;Kim, Jae-Gon;Baik, Byeong-Ju
    • Journal of the korean academy of Pediatric Dentistry
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    • v.39 no.3
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    • pp.249-256
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    • 2012
  • Cone beam computed tomography (CBCT) has become widely available in recent years and is recognized as an important diagnostic tool for varies disease and condition of the orofacial structure. Clinician is easy to determine adequate treatment plan for pediatric patients by using CBCT. CBCT is used in Chonbuk National University Dental Hospital since 2005. This research presents clinical application of CBCT on patients visiting department of pediatric dentistry in Chonbuk National University Dental Hospital from Jan, 2005 to July, 2011. 1. Total number of patients taken CBCT is 252, and total number of area taken CBCT is 279. 2. An age group form 9 years to 12 years showing 53% was highest and percentage of 6~8 years showed 24%. 3. Chief complaints for CBCT taking are position and shape of impacted teeth (49.1%), mesiodens (19.4%), supernumerary teeth (7.9%), position and root canal shape of erupting teeth (7.2%), cyst (5.4%), inflammatory lesion (3.9%), odontoma (3.9%), tumor (2.2%), and et al. 4. Treatments are extraction (29.7%), orthodontic traction and leveling (24.0%), follow up (16.5%), refer to other professional part (11.5%), endodontic treatment (3.9%), surgical removal (2.9%), malsupialization (3.9%), enucleation (1.1%), and fail to follow up (5.0%), and et al.