• Korean Journal of Medicinal Crop Science
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• v.18 no.1
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• pp.28-33
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• 2010

Glycyrrhiza uralensis (Leguminosae) is a well-known herbal medicine that has long been valued as a demulcent to relieve inflammatory disorders. To compare the influence of different solvents on the anti-inflammatory efficacy of G. uralensis, we measured the inhibition of pro-inflammatory mediators such as NO, TNF-$\alpha$, and $PGE_2$ in lipopolysaccharide (LPS)-stimulated mouse macrophage RAW 264.7 cells by extracts produced using different solvents (water, methanol, ethanol, or n-hexane). The results showed that methanol was the most effective solvent for the inhibition of both NO and $PGE_2$ production in RAW 264.7 cells. However, there was no difference among the extracts for inhibition of TNF-$\alpha$. Further study must be performed for the analysis of correlation between the anti-inflammatory activity of extracts produced using different solvents and the content of major bioactive compounds in G. uralensis, such as glycyrrhizin and liquiritin. The present study suggests that methanol may be a more appropriate solvent of G. uralensis than other solvents (water, ethanol, and n-hexane) to yield the greatest anti-inflammatory activity for food additives and medicine.

• Korean Journal of Human Ecology
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• v.24 no.6
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• pp.859-871
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• 2015

The purpose of this study is to examine the influence of the pigment characteristic and changes in dying conditions on the dying properties by extracting Chelidonium majus var. asiaticum using distilled water and ethanol as solvents. Changes in dying conditions include variations in dye concentrations, dyeing temperatures and time on dye uptake, and K/S Value was compared according to these changes. Additionally, color changes were observed through mordant. Ultraviolet-visible spectrum was used to investigate the pigment characteristic, and as a result, tannin was identified in distilled-water-extract, whereas berberine and chlorophyll were identified in ethanol-extract. In FT-IR analysis, tannin in distilled-water-extract was verified as hydrolyzable tannin. For ethanol extract, chlorophyll was verified through absorption band of C-H, which is aliphatic spectrum around $2920cm^{-1}$ and $2850cm^{-1}$. From GC/MS analysis, oil components as well as terpine compounds were detected in ethanol-extract, and this, in turn, brings expectation regarding functionality. When dying in silk, dye uptake increased as concentration of the extract increased, and the optimum dyeing temperature and time were $40^{\circ}C$ and 60 minutes respectively. Dyed fabrics' colors were all basically Y-series colors, and adjustment in brightness and revelation of khaki color were also available depending on the type of the mordant. Color fastness, except for washing fastness, was good in silk dyed with distilled-water-extract. Thus, it can be concluded that by selecting the right extracting method and by doing proper dyeing and mordant according to the needs, these dyed fabrics can be used as eco-friendly, functional clothing material.

• Biomedical Science Letters
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• v.23 no.4
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• pp.388-394
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• 2017

Recently, coffee is the most popular beverage for modern people. A great number of substances are found in coffee beans and have been studied for many years such as aliphatic and aromatic compounds. However, studies on the physiological activity of coffee extracts are insufficient. This study was performed to determine the contents of caffeine and chlorogenic acid in coffee extracts according to the solvent and to investigate the physiological activity of coffee extracts. Coffee extracts were extracted by ultrasonication method with various types of solvents including distilled water, ethanol, and other organic solvents under $50^{\circ}C$ and $80^{\circ}C$. The contents of caffeine and chlorogenic acid in coffee extracts were determined by Liquid Chromatography-Mass Spectrometry (LC-MS). Also, cytotoxic and antioxidative effects of coffee extracts were evaluated with MTT and DPPH assays to analyze the physiological activity. As a result, it was confirmed that caffeine and chlorogenic acid contents were extracted in distilled water with the highest rate. Antioxidative activity was observed below 10-fold dilute of coffee extracts, however cytotoxicity was not observed. In conclusion, distilled water was the best solvent for extracting caffeine and chlorogenic acids from coffee bean with ultrasonication and these coffee extracts are less cytotoxic in human skin cell lines and have antioxidant effect.

• YAKHAK HOEJI
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• v.44 no.2
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• pp.135-140
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• 2000

Determination of Aconitum alkaloids in processed Buza (Cho-0, Salted Buza, Moist-heating Buza, Limed Buza), which had been prepared from the raw tubers of Aconitum chiisanenseb(Ranunculaceae), was established using visible spectrophotometry and high-performance liquid chromatography (HPLC) method especially for Aconitine analysis. Aconitum alkloids were reacted with tetra- thiocy-anatocobalt[II] complex ion to form a stable ion pair. The reaction product was insoluble in water but freely soluble in several organic solvents. 1.2-Dichloroethane was the best extracting solvent among the examined solvents. Spectrophotometry of Aconitum alkaloids at nax. 625 was carried out. The HPLC method for aconitine was carried out using Radial PAK-CN column with gradient solvent system by solvent mixture of acetonitrile and phosphate buffer (pH 3.0) at 4$0^{\circ}C$ and 254 nm. Linear relationship was found between absorbance response and concentration of aconitine in range of 0.45 mM~0.9 mM ($r^2$=0.9949) by spectrophotometry and 0.3 mM~1.2mM($r^2$=0.9983) by HPLC method. These methods have been found to be suitable and reproducible for routine analysis of Aconitum alkaloids and its pharmaceutical preparations.

• The Korean Journal of Food And Nutrition
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• v.27 no.6
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• pp.1059-1066
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• 2014

In this study, the nutritional value, total polyphenol content, total flavonoid content, and antioxidant activity of freeze-dried Cedrela sinensis tender leaf powder were examined. Among the nutritional values, the crude protein, crude fiber, calcium, and potassium were abundantly present in Cedrela sinensis. The Cedrela sinensis powder was extracted with two solvents, 70% ethanol and distilled water (D.W.), to evaluate its functional properties. Total polyphenol and flavonoid contents were measured in the two different extracts, and the extracts were screened for their potential antioxidant activities using tests such as DPPH radical scavenging activity, ferric reducing antioxidant power (FRAP), and ABTS radical scavenging assay. Although both extracts exhibited good antioxidant activities against trolox, the ethanolic extract exhibited higher antioxidant activities than the D.W. extract. These results indicated that the Cedrela sinensis powder is a high-valued food ingredient and the extraction with 70% ethanol will be useful as a nutritional source with natural antioxidant activities.

• Journal of Conservation Science
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• v.36 no.2
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• pp.103-111
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• 2020

To compare the cleaning effect of solvents such as water and ethanol used to clean lacquer relics, the components extracted with various solvents were analyzed. A freshly dried lacquer sample and a lacquer sample exposed to ultraviolet radiation for 24 days were treated with water, ethanol, acetone, and hexane, and the dissolved material was detected by pyrolysis-gas chromatography-mass spectrometry. The amount extracted was significantly higher in acetone and ethanol than in hexane, a nonpolar solvent. Water, a highly polar solvent, was relatively efficient for extracting low-molecular-weight materials. After experiencing 24 days of ultraviolet radiation, the lacquer exhibited a significant increase of extracted materials compared with the nonexposed one. This may be due to the degradation of the urushiol chain in the form of polymers, resulting in the formation of low-molecular-weight polar substances, including dicarboxylic acids. In addition to the deterioration status of lacquer relics, such solvent extraction properties will be a crucial consideration in selecting the appropriate cleaning solvent.

• Journal of the Korean Home Economics Association
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• v.41 no.1
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• pp.249-257
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• 2003

For the efficiency in extracting gromwell colorant, methanol, ethanol and aceton were used as solvents. Also, to compare the results when dyed in various conditions and on various fabrics, the ratio of water-dye mixture, pH, temperature, and varieties of fabrics -wool, silk, cotton, nylon, ramie- were selected. L, a, b, ΔE, munsell and K/S value of each sample was measured and compared for the practical use. Water-dye mixture of 5:5 ratio showed the optimum dyeability. Not to mention wool and silk, but also cotton showed a satisfying dying result at the acidity of pH4. As the temperature increased, the dyeability of all three fabrics improved greatly. Especially, wool showed the highest improvement in terms of dyeability as the temperature increased. K/S values for wool and nylon showed superior results to the others in the gromwell colorant. The resulting color turned out somewhat different depending on the solvents used and the sort of fabric that was tested on. When the gromwell colorant extracted with methanol or ethanol was applied, the color of fabrics came out as purple or purpleblue. When the colorant extracted with acetone was used, the color of fabrics came out to be redpurple. The colorfastness to light showed low glades regardless to whichever solvents were used. The grades of colorfastness to laundering were recorded low numerical values, and its record became even lower when the gromwell colorant extracted with aceton was applied. The staining grade of the colorfastness to laundering showed a good grade in the range of 4 to 5. All the dyed fabrics showed a excellent drycleaning fastness.

• Journal of Applied Biological Chemistry
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• v.55 no.3
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• pp.149-156
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• 2012

The effects of solvent type and concentration, solid/liquid ratio, extraction time and repeated extraction on recovery of keratinase from solid-state fermentation (SSF) of chicken feather by a local Streptomyces sp. NRC 13S were investigated in order to establish the experimental conditions for keratinase yield. Among solvents tested, 0.5% (v/v) glycerol was the best. Box-Behnken design was used to investigate the effect of relevant variables on keratinase recovery. The factors investigated were solid/liquid ratio (1:1.66-1:6.66 g/mL), glycerol concentration (0.5-5% v/v) and repeated extraction (1-5 cycle). The results showed that the maximum recovery of keratinase (6933.3 U/gfs) was obtained using 0.5 (v/v) glycerol as extracting solvent, in a solid/liquid ratio of 1:5 and three extraction cycles.

• Textile Coloration and Finishing
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• v.13 no.5
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• pp.10-10
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• 2001

The Purpose of this study was to investigate spectrophotometric characteristics of Korean knotweed extracts. The properties were evaluated by it′s extracting solvent, effect of metallic ion, variations of pH values and effect of light exposure. The results were as following; The highest absorbance was found in methanol extract of Korean knotweed, while the lowest absorbance was carbon tetrachloride extract. The UV-vis. spectra of Korean knotweed colors in several solvents showed hypsochromic shift of n-π/sup ＊/ transition with the polarity of solvent. Absorbance and peak of UV-vis. spectra of Korean knotweed colors became lower and broader by addition of metallic ion. The light stability in irradiation with xenon ]amp of the color solution with Cu were higher than those of Al and Fe. The UV-vis. spectra of Korean knotweed extracts in various pH values showed bathochromic shift under alkaline condition, and their peaks disappeared after 5 hours exposure.

• Textile Coloration and Finishing
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• v.13 no.5
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• pp.298-305
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• 2001

The Purpose of this study was to investigate spectrophotometric characteristics of Korean knotweed extracts. The properties were evaluated by it's extracting solvent, effect of metallic ion, variations of pH values and effect of light exposure. The results were as following; The highest absorbance was found in methanol extract of Korean knotweed, while the lowest absorbance was carbon tetrachloride extract. The UV-vis. spectra of Korean knotweed colors in several solvents showed hypsochromic shift of $n->\pi^*$ transition with the polarity of solvent. Absorbance and peak of UV-vis. spectra of Korean knotweed colors became lower and broader by addition of metallic ion. The light stability in irradiation with xenon ]amp of the color solution with Cu were higher than those of Al and Fe. The UV-vis. spectra of Korean knotweed extracts in various pH values showed bathochromic shift under alkaline condition, and their Peaks disappeared after 5 hours exposure.