• Parasites, Hosts and Diseases
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• 제51권2호
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• pp.247-253
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• 2013

Neospora caninum is the etiologic agent of bovine neosporosis, which affects the reproductive performance of cattle worldwide. The transmembrane protein, NcSRS2, and dense-granule protein, NcGRA7, were identified as protective antigens based on their ability to induce significant protective immune responses in murine neosporosis models. In the current study, NcSRS2 and NcGRA7 genes were spliced by overlap-extension PCR in a recombinant adenovirus termed Ad5-NcSRS2-NcGRA 7, expressing the NcSRS2-NcGRA7 gene, and the efficacy was evaluated in mice. The results showed that the titer of the recombinant adenovirus was $10^9TCID_{50}/ml$. Three weeks post-boost immunization (w.p.b.i.), the IgG antibody titer in sera was as high as 1:4,096. IFN-${\gamma}$ and IL-4 levels were significantly different from the control group (P<0.01). This research established a solid foundation for the development of a recombinant adenovirus vaccine against bovine N. caninum.

• 한국포장학회지
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• 제23권3호
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• pp.133-141
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• 2017

본 논문에서는 신선도 유지 기능을 부여한 새로운 형태의 산화생분해성 필름 개발에 관하여 서술하였다. 생분해 촉매제로 유기 금속염, 유기산, 불포화 지방산등을 함유한 산화생분해성 및 항균신선도기능을 부여한 M/B를 각각 제조한 후, 이를 플라스틱 레진에 첨가하여 항균 및 신선도 기능의 산화생분해 복합필름(AOB film)을 제작하였다. 제조된 항균신선도 A M/B의 항균력 시험은 진탕 플라스크 방법을 사용하여 농도별 시험을 실시하였다. A MB 5% 첨가한 AOB 필름은 별도로 필름 밀착법을 통해 제조하여 실험한 결과, 필름의 항균작용이 매우 우수함을 확인할 수 있었다. 자두를 대상으로 한 신선도 유지기능 평가 결과, A M/B 5% 첨가한 AOB 필름이 대조군 필름에 비하여 신선도 유지 효과가 우수하였다. 또한 산화생분해성을 평가하기 위하여, UV 340 nm로 처리한 필름의 인장강도 및 신장율을 측정한 결과, AOB 필름의 물성 감소율이 우수하였으며, 이는 산화생분해 특성을 갖는 것을 의미한다. 결론적으로 항균 및 신선도 기능의 산화생분해성(AOB) 복합필름은 식품 유통과정에서 발생할 수 있는 식품의 부패를 방지하는 측면에서 긍정적인 효과를 가져올 수 있을 것으로 판단되어진다.

• 한국식품저장유통학회지
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• 제7권1호
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• pp.57-62
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• 2000

Ginseng was stored at a temperature lower than the freezing point after a treatment of freezing point depressing agents to extend its freshness. Respiration rate at freezing point of ginseng, -1.7${\pm}$0.1$^{\circ}C$, was inhibited 92% and 97% compared with those stored at 5$^{\circ}C$or 20$^{\circ}C$ , respectively. Sorbitol solution chosen as a freezing point depressing agent lowered the freezing point of ginseng to about -3.0$^{\circ}C$. Ginsengs treated with the sorbitol solution and packaged with 0.06mm LDPE was stored at -2$^{\circ}C$ , and the quality change was then compared with ginsengs stored at 0$^{\circ}C$ and 5$^{\circ}C$. Weight loss of ginsengs stored at -2$^{\circ}C$ for 100days was 1.5%, which is about 2.6times less than those stored at 5$^{\circ}C$. However, there were no significant difference between the ginsengs stored at -2$^{\circ}C$ and at 0$^{\circ}C$(1.9%). Spoilage rate of the ginsengs was 100% after 50 days of storage at 5$^{\circ}C$ and 25% after 100days at 0$^{\circ}C$respectively. but that of ginsengs stored at -2$^{\circ}C$ was 13%, which was half than that of ginsengs stored at 0$^{\circ}C$. Firmness and amount of monoscaccharides in ginsengs were decreased during storage at 5 or 0$^{\circ}C$ but ginsengs stored at -2$^{\circ}C$ showed better firmness and an increase in monosaccharides such as fructose and glucose. From above, when ginseng treated with freezing pont depressing agents were stored at -2$^{\circ}C$, the shelf life was extended to 2 or 3 times longer than those that were stored at 5 or 0$^{\circ}C$, respectively.

• Archives of Pharmacal Research
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• 제26권1호
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• pp.76-82
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• 2003

Drug releasing porous poly($\varepsilon$-caprolactone) (PCL)-chitosan matrices were fabricated for bone regenerative therapy. Porous matrices made of biodegradable polymers have been playing a crucial role as bone substitutes and as tissue-engineered scaffolds in bone regenerative therapy. The matrices provided mechanical support for the developing tissue and enhanced tissue formation by releasing active agent in controlled manner. Chitosan was employed to enhance hydrophilicity and biocompatibility of the PCL matrices. PDGF-BB was incorporated into PCL-chitosan matrices to induce enhanced bone regeneration efficacy. PCL-chitosan matrices retained a porous structure with a 100-200 $\mu$m pore diameter that was suitable for cellular migration and osteoid ingrowth. $NaHCO_3$ as a porogen was incorporated 5% ratio to polymer weight to form highly porous scaffolds. PDGF-BB was released from PCL-chitosan matrices maintaining therapeutic concentration for 4 week. High osteoblasts attachment level and proliferation was observed from PCL-chitosan matrices. Scanning electron microscopic examination indicated that cultured osteoblasts showed round form and spread pseudopods after 1 day and showed broad cytoplasmic extension after 14 days. PCL-chitosan matrices promoted bone regeneration and PDGF-BB loaded matrices obtained enhanced bone formation in rat calvarial defect. These results suggested that the PDGF-BB releasing PCL-chitosan porous matrices may be potentially used as tissue engineering scaffolds or bone substitutes with high bone regenerative efficacy.

• Journal of Microbiology and Biotechnology
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• 제22권7호
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• pp.883-888
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• 2012

The efficacy of cowdung, Bangladesh Institute of Nuclear Agriculture (BINA)-biofertilizer, and Bangladesh Agricultural University (BAU)-biofungicide, alone or in combination, was evaluated for controlling foot rot disease of lentil. The results exhibited that BINA-biofertilizer and BAU-biofungicide (peat soil-based Rhizobium leguminosarum and black gram bran-based Trichoderma harzianum) are compatible and have combined effects in controlling the pathogenic fungi Fusarium oxysporum and Sclerotium rolfsii, which cause the root rot of lentil. Cowdung mixing with soil (at 5 t/ha) during final land preparation and seed coating with BINA-biofertilizer and BAU-biofungicide (at 2.5% of seed weight) before sowing recorded 81.50% field emergence of lentil, which showed up to 19.85% higher field emergence over the control. Post-emergence deaths of plants due to foot rot disease were significantly reduced after combined seed treatment with BINA-biofertilizer and BAU-biofungicide. Among the treatments used, only BAU-biofungicide as the seed treating agent resulted in higher plant stand (84.82%). Use of BINA-biofertilizer and BAU-biofungicide as seed treating biocontrol agents and application of cowdung in the soil as an organic source of nutrient resulted in higher shoot and root lengths, and dry shoot and root weights of lentil. BINA-biofertilizer significantly increased the number of nodules per plant and nodules weight of lentil. Seeds treating with BAU-biofungicide and BINA-biofertilizer and soil amendment with cowdung increased the biomass production of lentil up to 75.56% over the control.

• 식물병연구
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• 제18권1호
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• pp.40-44
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• 2012

자두 검은점무늬병에 걸린 식물체로부터 순수분리된 X. arboricola pv. pruni 균을 NA배지에서 배양한 후 국내재배 핵과류에 분무접종한 결과 자두, 매실, 살구에 있어서는 과실에 뚜렷한 병원성을 나타내었으나 복숭아 과실은 저항성을 나타내었다. 특히 매실과 살구에서는 극심한 초기 과민성반응이 나타났는데 과실의 50% 정도가 생장 정지되어 낙과되는 현상을 보였으며 병반의 크기는 자두와는 달리 크게 진행되지 않고 많은 점무늬를 형성하는 병반형태를 나타내었고 복숭아에서는 과실병징이 나타나지 않았다. 접종된 여름철 신초가지에서 자두 포모사품종에서는 도관부까지 궤양조직이 형성되었으나 살구 그리고 매실에서는 체관부일부에서만 궤양조직이 형성되어 분리된 병원균은 자두 포모사품종에 특이적인 감염조직을 형성함을 알 수 있었다.

• 농업과학연구
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• 제47권4호
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• pp.1071-1081
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• 2020

Essential oils (EOs) have been shown to be plant-extracted antimicrobial agents. However, there are limited studies investigating the efficacy of EOs against pathogens. Among them, tea tree oil (TTO) is extracted from Melaleuca alternifolia, which is also used as an antifungal agent. In this study, the effect of TTO was investigated on the suppression of melon powdery mildew caused by Podosphaera xanthii and tomato leaf mold disease caused by Passalora fulva. Both powdery mildew and leaf mold diseases were significantly suppressed by a spray of TTO. Eighty percent of powdery mildew and 81% of leaf mold disease of the control value were suppressed by 0.5% TTO liquid, when sprayed 3 times every 7 days on the melon and tomato leaves. Inhibition of mycelial growth was also greatly affected by different concentrations of TTO against four different fungal pathogens. Ninety-eight percent of Pseudocercospora fuligena, 97% of P. fulva, 95% of Botrytis cinerea, and 94% of Phytophthora infestans mycelial growth were inhibited by 0.2% to 1.0% of TTO contained in plate media, respectively. However, phytotoxicity in plants by the TTO treatments was revealed when melon and tomato leaves were sprayed with a 1% and 2% concentration of TTO, respectively. Therefore, our findings show that TTO has high antifungal effects against various plant pathogens that occur during crop cultivation. We also suggest that when applying TTO to plant leaves, it is necessary to establish an accurate treatment concentration for different crops.

• The Korean Journal of Physiology and Pharmacology
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• 제18권5호
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• pp.441-446
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• 2014

Ursolic acid (UA), a type of pentacyclic triterpenoid carboxylic acid purified from natural plants, can promote skeletal muscle development. We measured the effect of resistance training (RT) with/without UA on skeletal muscle development and related factors in men. Sixteen healthy male participants (age, $29.37{\pm}5.14$ years; body mass index=$27.13{\pm}2.16kg/m^2$) were randomly assigned to RT (n=7) or RT with UA (RT+UA, n=9) groups. Both groups completed 8 weeks of intervention consisting of 5 sets of 26 exercises, with 10~15 repetitions at 60~80% of 1 repetition maximum and a 60~90-s rest interval between sets, performed 6 times/week. UA or placebo was orally ingested as 1 capsule 3 times/day for 8 weeks. The following factors were measured pre-and post-intervention: body composition, insulin, insulin-like growth factor-1 (IGF-1), irisin, and skeletal muscle strength. Body fat percentage was significantly decreased (p<0.001) in the RT+UA group, despite body weight, body mass index, lean body mass, glucose, and insulin levels remaining unchanged. IGF-1 and irisin were significantly increased compared with baseline levels in the RT+UA group (p<0.05). Maximal right and left extension (p<0.01), right flexion (p<0.05), and left flexion (p<0.001) were significantly increased compared with baseline levels in the RT+UA group. These findings suggest that UA-induced elevation of serum irisin may be useful as an agent for the enhancement of skeletal muscle strength during RT.

• 생명과학회지
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• 제19권5호
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• pp.684-687
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• 2009

핵과류 과수에 세균성구멍병을 일으키는 Xanthomonas arboricola pv. pruni는 집단의 다양성이 적은 것으로 알려지고 있다. 우리나라에서 분리된 X. arboricola pv. pruni의 유전적 특성을 조사하기 위하여 동일한 16S rDNA 염기서열을 갖는 X. arboricola pv. pruni의 type strain인 LMG852, 일본 균주 MAFF301420, 우리나라 균주 XWD1의 세 균주를 대상으로 세 개 유전자 부위의 DNA 염기서열과 RAPD 분석을 실시하였다. 그 결과 ITS와 glnA, atpD의 염기서열은 세 균주가 동일하였다. 그러나 756 염기로 구성된 atpD의 염기서열은 GenBank에 등록된 프랑스균주와 5곳에서 차이가 있었다. 40개의 random primer를 사용한 RAPD 결과는 우리나라와 일본균주는 동일한 밴드 패턴을 보이나 대표균주와는 다른 양상을 보였다. 이러한 사실은 우리나라와 일본의 X. arboricola pv. pruni의 개체군은 매우 가까워 유전적 다양성이 낮은 것으로 보이며 유럽균주와는 다른 기원과 전파 경로를 갖는 것으로 생각된다.

• Applied Biological Chemistry
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• 제48권2호
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• pp.150-154
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• 2005

천연식물자원으로부터 생물농약을 개발하기 위하여 점박이응애(Tetranichus urticae), 목화진딧물(Aphis gossypii)과 복숭아혹진딧물(Myzus persicae)에 대해 푸른은행잎 추출물(GG-W80)과 노란은행잎 추출물(YG-W80)의 살충효과와 기피효과를 leaf disk법과 유묘검정법에 의해 조사하였다. 점박이응애에 대한 살충효과는 GG-W80이 98.3%로 YG-W80의 20.0%보다 우수하였다. 점박이응애에 강한 살충효과를 보이는 생리활성물질은 high performance liquid chromatography(HPLC)를 이용해 동정하였다. 푸른은행잎 추출물에서는 각각 bilobalide $611\;{\mu}g/kg$, ginkgolide A $37\;{\mu}g/kg$와 ginkgolide B $243\;{\mu}g/kg$이 동정되었으며, 노란은행잎 추출물에서는 bilobalide $214\;{\mu}g/kg$와 ginkgolide B $46\;{\mu}g/kg$이 동정되었으나 ginkgolide A는 확인되지 않았다. 이상의 결과는 은행잎에 존재하는 terpene계 화합물인 bilobalide와 ginkgolide A, B가 점박이응애에 살충력을 갖고 있음을 알수 있으며, 이들 성분을 포함한 은행잎 추출물이 점박이응애에 대한 생물학적 방제 가능성을 가지고 있음을 시사하였다.