• Korean Journal of Clinical Laboratory Science
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• v.48 no.3
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• pp.210-216
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• 2016

Recently, there has been a considerable increase in the prevalence of CTX-M type extended-spectrum ${\beta}$-lactamase (ESBL)-producing E. coli isolates worldwide, including Korea. To investigate the ESBL genes in the E. coli strains isolated from a university hospital in the Chungcheong area, a study was conducted using PCR amplification and nucleotide sequence analysis of the amplified products to detect the plasmid mediated quinolone resistance (PMQR) genes in ESBL producing E. coli isolates. The number of CTX-M-14 producing isolates was 25 (16.0%) isolates, and of them, 9 (5.8%) isolates also produced CTX-M-15. All CTX-M type ESBL producing E. coli isolates showed resistance to cefotaxime. Twelve (48%) CTX-M type ESBL producing E. coli isolates contained the PMQR genes, 8 contained qnrS1, and 8 contained aac(6')-Ib-cr. Four isolates harbored both qnrS1 and aac(6')-Ib-cr genes. In our study, we confirmed that the plasmid mediated antimicrobial resistant determinants-the ESBL and PMQR genes-were distributed in the E. coli isolates. To prevent further spreading of the resistant genes among the E. coli isolates, consistent effort is required to investigate and monitor the resistant genes.

• Journal of the Korean Society for Precision Engineering
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• v.10 no.1
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• pp.34-41
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• 1993

SUS304 is wellknown as difficult-to-machine materials. It is easy to appear workhardened, and workhardening is one of the causes of groove wear on the tool. In this paper, the author would like to compare the width of flank wear with that of groove wear, and to find whether the groove wear can be used as a criterion of a tool life. The design of the twelve tests provides three levels for each variable (speed: 200m/min, 118m/min, 70m/min; feed: 0.3mm/rev, 0.17mm/rev, 0.1mm/rev; depth of cut: 0.4mm, 0.28mm, 0.2mm). The study of tool-life testing by statistical technique follows usual most scientific sequence. So the tool-life predicting equation is calculated by the method of least squares. The overall adequacy of the model can be verified by the analysis of variance. The results obtained are as follows : 1) When SUS304 is cut in 200(m/min), the width of flank wear is much larger than that of groove wear. 2) In cutting speed 118m/min, flank wear is a little larger than groove wear and in the cutting speed 70m/min, the latter is a little larger so that it is reasonable to determine the tool life according the crierion by groove wear in the low cutting speed (less than 70m/min). 3) Owing to the burr the depth of engagement along the cutting edge is extended toward the shank.

• Korean Journal of Clinical Laboratory Science
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• v.45 no.2
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• pp.43-47
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• 2013

This study was designed to evaluate the prevalence of ESBL genes and monitor antimicrobial resistance pattern in Escherichia coli, isolated from a hospital and a community. We tested 200 E. coli strains isolated in the hospitals and community in Chungcheong area from January to March 2012. Antimicrobial susceptibilities were tested by using the disk diffusion method. A search for ESBL genes was conducted by PCR amplification, and the genotypes were determined by direct nucleotide sequence analysis of the amplified products. An Epidemiologic study was performed by repetitive extragenic palindromic sequence-based PCR (REP-PCR). The percentage of ESBL-producing isolates was 17% for hospital associated E. coli and 11% for community associated E. coli. The ESBL gene sequencing results showed that the most common ESBL in E. coli was CTX-M-14 (19/28), followed by CTX-M-15 (9/28). The REP-PCR study also showed the genetic diversity, but there was no difference between the hospital and community associated E. coli. In this study, the most common types of class A ESBLs identified were CTX-M in the hospital and the community in Chungcheong area. ESBL-producing E. coli isolates showed diverse clonality.

• KSBB Journal
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• v.17 no.3
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• pp.283-289
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• 2002

The present study was aimed at investigating the metabolic regulation of insulin-like growth factor-I(IGF-I) expression in fasting animals. The expression of IGF-I gene was determined by a solution hybridization/RNase protection assay using total RNA from control, 4d-fasting, and 2d-fasting-refed rats. The levels of IGF-I transcripts were reduced in 4d-fasting than in control by decreasing its transcriptional rate, which was measured through nuclear nun-on assay. DNase I footprinting, which was performed using nuclear extracts from fasting rat, demonstrated protein binding to a sequence that extended from +179 to +210 (termed region B). These data suggest that the expression of IGF-I is transcriptionally regulated through DNA-liver enriched protein binding in a sequence which is located downstream from major transcription initiation site of IGF-I gene.

• Macromolecular Research
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• v.17 no.12
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• pp.1003-1009
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• 2009

Copolymers of N-vinylpyrrolidone (NVP) comonomer with styrene (St), hydroxypropyl methacrylate (HPMA) and carboxyphenyl maleimide (CPMI) were synthesized by free radical polymerization using 2,2'-azobisisobutyronitrile (AIBN) initiator in 1,4-dioxane solvent. The copolymers formed were characterized by FTIR, $^1H$ NMR and $^{13}C$ NMR techniques and their thermal properties were studied by DSC and TGA. Copolymer composition was determined by $^1H$ NMR and/or by elemental analysis and monomer reactivity ratios (MRR) were estimated by the linear methods of Kelen-Tudos (K-T) and extended Kelen-Tudos (EK-T) and the non-linear approach. Copolymers of St and HPMA with NVP formed blocks of one of the monomer units, whereas alternating copolymers were obtained in CPMI-NVP, depending upon the side chain substitution. The MRR values are discussed in terms of monomer structural properties such as electronegativity and electron delocalization. The sequence distribution of monomers in the copolymers was studied by statistical method based on the average reactivity ratios obtained by EK-T method.

• Bulletin of the Korean Chemical Society
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• v.19 no.1
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• pp.57-62
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• 1998

We have synthesized a 17-residue peptide with the amino acid sequence RQMKEDAKGKSEEELAD corresponding to residues 84-100 of chicken skeletal troponin C. This stretch of the protein sequence is in the middle one-third of the 32-residue 9-turn α-helix that connects the two globular domains of the dumbell-shaped molecule and includes the D/E linker helix. We describe here the solution conformation of the helix linker as studied by circular dichroism (CD) and two-dimensional nuclear magnetic resonance (2-D NMR) spectroscopy. The NOE connectivities together with the vicinal $^3J_{N{\alpha}}$ coupling constants suggest that the peptide exists in a fast conformational equilibrium among several secondary structure: a nascent helix near the N-terminus, a helix, and a substational population of extended and random coil forms. In addition, two interresidue α-α NOEs are observed suggesting a bent structure with a bend that includes the single glycine in position 92. These results are consistent with the ideas that in neutral solution the D/E linker region of the central helix in troponin C can adopt a helical conformation and the central helix may have a segmental flexibility around Gly 92.

• The Bulletin of The Korean Astronomical Society
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• v.43 no.2
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• pp.59.4-60
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• 2018

Ultra-diffuse galaxies (UDGs) are an unusual galaxy population. They are ghostlike galaxies with fainter surface brightness than normal dwarf galaxies, but they are as large as MW-like galaxies. The key question on UDGs is whether they are 'failed' giant galaxies or 'extended' dwarf galaxies. To answer this question, we study UDGs in massive galaxy clusters. We find an amount of UDGs in deep HST images of three Hubble Frontier Fields clusters, Abell 2744 (z=0.308), Abell S1063 (z=0.347), and Abell 370 (z=0.374). These clusters are the farthest and most massive galaxy clusters in which UDGs have been discovered until now. The color-magnitude relations show that most UDGs have old stellar population with red colors, while a few of them show bluer colors implying the existence of young stars. The stellar masses of UDGs show that they have less massive stellar components than the bright red sequence galaxies. The radial number density profiles of UDGs exhibit a drop in the central region of clusters, suggesting some of them were disrupted by strong gravitational potential. Their spatial distributions are not homogeneous, which implies UDGs are not virialized enough in the clusters. With virial masses of UDGs estimated from the fundamental manifold, most UDGs have M_200 = 10^10 - 10^11 M_Sun indicating that they are dwarf galaxies. However, a few of UDGs more massive than 10^11 M_Sun indicate that they are close to failed giant galaxies.

• Journal of Microbiology and Biotechnology
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• v.34 no.5
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• pp.1101-1108
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• 2024

Earlier studies have validated the isolation of extended-spectrum beta-lactamase-producing Salmonella (ESBL-Sal) strains from food. While poultry is recognized as a reservoir for Salmonella contamination, pertinent data regarding ESBL-Sal remains limited. Consequently, the Ministry of Food and Drug Safety has isolated Salmonella spp. from retail meat and evaluated their antibiotic susceptibility and genetic characteristics via whole-genome sequencing. To further elucidate these aspects, this study investigates the prevalence, antibiotic resistance profiles, genomic characteristics, and homology of ESBL-Sal spp. obtained from livestock-derived products in South Korean retail outlets. A total of 653 Salmonella spp. were isolated from 1,876 meat samples, including 509 beef, 503 pork, 555 chicken, and 309 duck samples. The prevalence rates of Salmonella were 0.0%, 1.4%, 17.5%, and 28.2% in the beef, pork, chicken, and duck samples, respectively. ESBL-Sal was exclusively identified in poultry meat, with a prevalence of 1.4% in the chicken samples (8/555) and 0.3% in the duck samples (1/309). All ESBL-Sal strains carried the bla_CTX-M-1 gene and exhibited resistance to ampicillin, ceftiofur, ceftazidime, nalidixic acid, and tetracycline. Eight ESBL-Sal isolates were identified as S. Enteritidis with sequence type (ST) 11. The major plasmid replicons of the Enteritidis-ST11 strains were IncFIB(S) and IncFII(S), carrying antimicrobial resistance genes (β-lactam, tetracycline, and aminoglycoside) and 166 virulence factor genes. The results of this study provide valuable insights for the surveillance and monitoring of ESBL-Sal in South Korean food chain.

• Kyungpook Mathematical Journal
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• v.58 no.1
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• pp.183-202
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• 2018

${\Delta}-moves$ are closely related with a Vassiliev invariant of degree 2. For classical knots, M. Okada showed that the second coefficients of the Conway polynomials of two knots differ by 1 if the two knots are related by a single ${\Delta}-move$. The first author extended the Okada's result for virtual knots by using a Vassiliev invariant of virtual knots of type 2 which is induced from the Kauffman polynomial of a virtual knot. The arrow polynomial is a generalization of the Kauffman polynomial. We will generalize this result by using Vassiliev invariants of type 2 induced from the arrow polynomial of a virtual knot and give a lower bound for the number of ${\Delta}-moves$ transforming $K_1$ to $K_2$ if two virtual knots $K_1$ and $K_2$ are related by a finite sequence of ${\Delta}-moves$.

• 제어로봇시스템학회:학술대회논문집
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• 1992.10b
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• pp.569-572
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• 1992

This paper describes a new method of pseudorandom testing of a digital circuit by use of correlation method and a neural network. The authors have recently proposed a new method of fault diagnosis of logical circuit by applying a pseudorandom M-sequence to the circuit under test, calculating the crosscorrelation function between the input and the output, and comparing the crosscorrelation functions with the references. This method, called MSEC method, is further extended by using a neural network in order to not only detect the existence of faults but also find the place or location of the faults. An experiment by using a simple digital circuit shows enough applicability of this method to industrial testing of circuit board.