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Metabolic Regulation of Insulin-like Growth Factor-1 Expression  

안미라 (전남대학교 수의과대학 수의학과 및 생물공학연구소)
Publication Information
KSBB Journal / v.17, no.3, 2002 , pp. 283-289 More about this Journal
Abstract
The present study was aimed at investigating the metabolic regulation of insulin-like growth factor-I(IGF-I) expression in fasting animals. The expression of IGF-I gene was determined by a solution hybridization/RNase protection assay using total RNA from control, 4d-fasting, and 2d-fasting-refed rats. The levels of IGF-I transcripts were reduced in 4d-fasting than in control by decreasing its transcriptional rate, which was measured through nuclear nun-on assay. DNase I footprinting, which was performed using nuclear extracts from fasting rat, demonstrated protein binding to a sequence that extended from +179 to +210 (termed region B). These data suggest that the expression of IGF-I is transcriptionally regulated through DNA-liver enriched protein binding in a sequence which is located downstream from major transcription initiation site of IGF-I gene.
Keywords
Insulin-like growth factor-1; fasting; DNA-protein binding; C/EBP isoform;
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