• Biomedical Science Letters
• /
• v.17 no.3
• /
• pp.185-190
• /
• 2011

Adenovirus type 5 (Ad5) vectors have been used for gene transfer to a wide variety of cell types in vivo and in vitro. The advantages of adenovirus vectors include the high titer of virus readily obtained in large scale preparations, their ability to transduce dividing and non dividing cells, and the high level of transgene expression. Since adenovirus vectors do not integrate in host cell DNA, there is a lack of insertional mutagenesis. However, many human tumor cells lack expression of the adenovirus 5 receptors and contain areas of hypoxia. In order to identify the pattern of replication and gene expression of oncolytic adenovirus in hypoxic condition, multiple different fiber modified Ads (Ad5F/S11, Ad5F/S35, Ad5F/K7, Ad5F/K21, and Ad5F/RGD) was compared. The replication of all fiber modified adenovirus was inhibited in hypoxic condition in HEK 293 cells, but gene expression has variety on different tumor cell lines and the level of coxackievirus and adenovirus receptor (CAR) expression. These data suggest that CAR expression pattern and hypoxic condition of tumor are considered for optimal oncolytic adenovirus application.

• Journal of the Korean housing association
• /
• v.15 no.6
• /
• pp.15-25
• /
• 2004

This study examined how the characteristics of abstractive form among various contenporary housing architecture have been expressed. The conclusions were: First, abstractive characteristics and types related to from expression of contemporary housing architectures were minimal form and absolute form of geometrical abstraction, plastic form and atypical form of expressive abstraction and mechanical aesthetics of industrial abstraction. Second, the typological form expression characteristics in minimal expression related to geometrical abstraction were simplicity, purity, and the properties of matter, and the characteristics in absolute expression were overlapping, obliqueness and dispensability. On the other hand, plasticity and mobility of materials were distinctive in plastic form expression, and inclination, curve and asymmetry were distinctive in atypical expression. The distinctive nature of mechanical aesthetics related to industrial abstraction included transparency, simplicity and. the properties of matter Funhermore, the study aimed at the understanding of various from expressions showed up in contemporary housing architecture, revealing the aspects of abstractive form expression characteristics.

• Journal of Plant Biology
• /
• v.37 no.1
• /
• pp.17-25
• /
• 1994

Two potato (Solanum tuberosum L.) cultivars were transformed by Agrobacterium tumefaciens harboring cauliflower mosaic virus (CaMV) 35S promoter and $\beta$-glucuronidase (GUS) gene. Expression patterns of the CaMV 35S promoter according to tissue types and developmental stages, and genetic stability of GUS gene were investigated in the clonal progenies of transgenic potatoes. Kanamycin-resistant shoot emerged from tuber disc after 4 weeks of culture, and root was induced 6 weeks after culture on the selection medium. Shooting frequency of cvs. Superior and Dejima were 43% and 27%, respectively. Mature transformants and their clonal progenies showed no phenotypical abnormality. GUS activity was expressed primarily at parenchymatous cells of phloem tissue around the vascular cambium in the stem and root, and higher activity was found at the apical meristem of shoot, root and adventious shoot bud. GUS activity was higher at tubers of young explants than at stored tubers. These facts indicate that expression level of the CaMV 35S promoter differed according to tissue types and developmental stages of the organs. The GUS gene was stably inherited to each clonal progeny and normally expressed.

• Genomics & Informatics
• /
• v.20 no.1
• /
• pp.13.1-13.4
• /
• 2022

A major issue in the use of immune checkpoint inhibitors is their lack of efficacy in many patients. Previous studies have reported that the T cell inflamed signature can help predict the response to immunotherapy. Thus, many studies have investigated mechanisms of immunotherapy resistance by defining the tumor microenvironment based on T cell inflamed and non-T cell inflamed subsets. Although methods of calculating T cell inflamed subsets have been developed, valid screening tools for distinguishing T cell inflamed from non-T cell inflamed subsets using gene expression data are still needed, since general researchers who are unfamiliar with the details of the equations can experience difficulties using extant scoring formulas to conduct analyses. Thus, we introduce TcellInflamedDetector, an R package for distinguishing T cell inflamed from non-T cell inflamed samples using cancer gene expression data via bulk RNA sequencing.

• Human Ecology Research
• /
• v.52 no.4
• /
• pp.383-394
• /
• 2014

The purpose of this study is to analyze the expression types and aesthetic meanings of fashion photographs adopting parody storytelling. The expressions of parody storytelling in advertising into the following five 5 types: parody of literature, movies, TV programs, art, and social issues. Therefore, in this study, we analyzed fashion photographs adopting parody storytelling from literature, movies, TV programs, art, and social issues, in fashion magazines. The meanings of fashion photographs adopting parody storytelling are as follows: First, the photographs adopting parody storytelling create new meanings with respect to a changeable narrative by transforming the original. Second, the humor of deviation comes from fun and interest generated the play of intent transformation and reconstruction of the original. Third, the photographs adopting parody storytelling of social issues suggest current messages. There is a growing tendency toward the value and the meanings of storytelling, and the various contents that have become the motive of the parody have important meanings. In particular, the analysis of expression methods and meanings of storytelling will contribute to raising the brand image through an effective fashion photographs project strategy. A story can be used as a source of inspiration and can contribute various ideas for a fashion photo-shoot and for fashion marketing.

• Journal of the Korean Society of Costume
• /
• v.43
• /
• pp.41-52
• /
• 1999

This study is to compare and analyze two types of 'transparency' concept expressed in architecture and garment which has not only been an important theme in the post-modern expression of architeture but been shown in various types of concept. This study examined the characteristics of transparency and through theoretical studies divided them into four types; frankness(purity ambiguity comparison exposure and eroticism) Purity building would use the matrial which is transparent glasses for the expprssion of its purity and clothing would use the transparent matrial for the expression of the natural beauty of human body and the purity of human of itself. Ambiguity is expressed in the outer side of architecture by duplicating transparent materials and by phenomenon of optical illusion and inflection. On the contrary in garment it is shown in the outward formation of underclothing. Comparison in architecture is expressed by the use of void but opaque materials as well as solid but transparent materials. Extinction is expressed with the visual effect of context of the surrounding by the use of opaque glass and reflexive glass. In garment the effect of comparison is expressed by the use of mixing transparent materials and opaque materials. Building would express the inside of itself by the way of its exposure and clothing would also express its frankness through the human body is partly opened or clossed at the same thime that arises from eroticism of the way of the expression of human feelings.

• Journal of Fashion Business
• /
• v.22 no.5
• /
• pp.41-51
• /
• 2018

Cable knitting, one of the most representative structures of knitwear has been applied to a number of knit items in a variety of expressions every season. The purpose of this study is to analyze the various types of expressions applied with cable knitting techniques to make the necessary data for planning cable knit designs. The types of cable knitting were devide into two categories which are based on the cable knitting pattern and structure. Based on the pattern, firstly, formal type is a reproduction of traditional Aran knit style and is usually applied to a practical and comfortable timeless knit product. Secondly, informal type is irregular applied to patterns and directions to highlight the dynamics of cable patterns. Thirdly, contrasting type emphasizes the cable pattern by contrasting the color of the part of the cable pattern. Fourthly, figurative type is the expression of the conceptual motifs and using the cable knit pattern itself as motif. Based on the structure, firstly, open type is to emphasize patterns and spaces by utilizing open spaces created by the intersection of cabling stitches. Secondly, decorative type is to enhance the ornamental effect of cable knitting by various methods and other subsidiary materials. Thirdly, avant-garde type is applied in various ways with exaggerated volumes or textures using the three-dimensional effects of cable knitting. Through this study, we look forward to the possibility of expressing the unique aesthetic characteristics of each of the various knitting techniques.

• Biomedical Science Letters
• /
• v.19 no.4
• /
• pp.348-352
• /
• 2013

microRNAs (miRNAs) play pivotal roles in controlling cell proliferation and differentiation. miRNA expression in human is becoming recognized as a new molecular mechanism of carcinogenesis. microRNA-34a (miR-34a), a member of the p53 network, was found to be regulated in multiple types of tumor. The purpose of this study was to define roles of miR-34a expression in cervical intraepithelial neoplasia with human papillomavirus infection, and its relationship with p53 protein expression. This study was performed to analyze expression of miR-34a by using qRT-PCR, and to evaluate p53 protein expression by using immunohistochemistry in 40 cases. Down-regulation of miR-34a expression was detected in 27 (67.5%) out of 40 cases and Immunoreactivity for p53 was found in 17 (42.5%) out of 40 cases. Nineteen (82.6%) of the 23 cases with a negative p53 expression showed a down-regulation miR-34a expression, there was a significant associations between miR-34a and p53 protein expression (P=0.04). These results suggest that miRNA-34a expression tend to be reduced depending on the advanced histologic grade, and down-regulation of miR-34a expression might be associated with inactivation of p53 protein expression by human papillomavirus infection.

• Korean Journal of Microbiology
• /
• v.30 no.6
• /
• pp.488-494
• /
• 1992

Three clones containing mouse CD4 gene were prepared using AKR genomic cosmid library. The role of 6, 500 bp 5' flanking region of the first exon of the AKR CD4 gene in tissue or developmental stage specific expression of CD4 has been studied. The deletion constructs containing various amounts of CD4 5' flanking sequences were prepared, and they were transfected into the cell lines representing different cell types or developmental stages of CD4 expression. Study of the reporter gene expression revealed that at least 1, 700 bp of 5' flanking region did retain promoter activity for CD4 expression. This area did not seem to contain enhancer activity for a full expression of CD4. However, the putative promoter interacted with other tissue specific enhancer sequence and showed the tissue specificity of the enhancer element.

• BMB Reports
• /
• v.29 no.5
• /
• pp.474-480
• /
• 1996

We present a study of evolutionary changes in expression of actin genes among closely related sea urchin species that exhibit different modes of early development. For this purpose, polyclonal antisera raised against peptides from the carboxyl terminus of the HeCyI cytoskeletal actin of Heliocidaris erythrogramma were used. H. erythrogramma is a direct developing sea urchin that proceeds from embryonic to adult stages without an intervening feeding larval stage. Expression patterns of the CyI actin isoform were compared with those of Heliocidaris tuberculata and to a related sea urchin Strongylocentrotus purpuratus, which both produce a feeding pluteus larval stage. The CyI actin of all three species is expressed in the same cell types. However, its expression patterns have been changed with reorganization of early cell lineage differentiation, which is apparent among the three species. Thus. evolutionary changes in CyI actin gene expression patterns are correlated with not only phylogenetic relationship, but developmental mode. The implication of this observation is that evolutionary changes in expression patterns of histospecific genes may underlie the emergence of novel developmental processes.