• Applied Microscopy
• /
• 제51권
• /
• pp.20.1-20.12
• /
• 2021

We explored the link between mitochondrial biogenesis and mitochondrial morphology using transmission electron microscopy (TEM) in lymphoblasts of pediatric acute lymphoblastic leukemia (ALL) patients and compared these characteristics between tumors and control samples. Gene expression of mitochondrial biogenesis markers was analysed in 23 ALL patients and 18 controls and TEM for morphology analysis was done in 15 ALL patients and 9 healthy controls. The area occupied by mitochondria per cell and the cristae cross-sectional area was observed to be significantly higher in patients than in controls (p-value=0.0468 and p-value<0.0001, respectively). The mtDNA copy numbers, TFAM, POLG, and c-myc gene expression were significantly higher in ALL patients than controls (all p-values<0.01). Gene Expression of PGC-1α was higher in tumor samples. The analysis of the correlation between PGC-1α expression and morphology parameters i.e., both M/C ratio and cristae cross-sectional area revealed a positive trend (r=0.3, p=0.1). The increased area occupied by mitochondria and increased cristae area support the occurrence of cristae remodelling in ALL. These changes might reflect alterations in cristae dynamics to support the metabolic state of the cells by forming a more condensed network. Ultrastructural imaging can be useful for affirming changes occurring at a subcellular organellar level.

• 한국콘텐츠학회논문지
• /
• 제12권8호
• /
• pp.429-437
• /
• 2012

본 연구는 신체표현을 활용한 색깔과 화음, 다이내믹을 연결하여 일어나는 변화에 목적을 두고 있다. 본 연구를 통하여 영상미디어 음악치료 프로그램 모형개발을 위한 기초 자료 수집을 위해 심리적으로 화음과 다이내믹이 색깔에 변화를 준다는 것을 탐색하였다. 영상매체에서 음악은 비언어적 의사소통으로 많은 분야에서 사용된다. 특히 음과 색깔 관계의 심리적 변화를 통하여 가상과 현실의 갭을 줄이는 데에 가장 효과적인 도구이며 사용자는 메시지와 의미에 대해 우리의 감성을 유도한다. GIM (Method of Guided Imagery and Music)은 인간의 내면세계를 탐색하고 음악에 유도된 다양한 심상 경험을 통해 상징적 의미와 통찰로 자신을 이해하고 문제를 해결해가도록 하는 음악치료방법으로 인간의 내적세계를 광범위하게 다룬다. 본 연구를 통하여 영상미디어라는 도구를 기존의 음악치료에서 사용되어져 왔던 수동적이고 소극적인 음악 감상 활용방법에서 탈피하고 직접 몸으로 체험하는 능동적이고 적극적인 음악치료 프로그램 모형을 적용시켜 나아가 오늘날 급격히 변화하는 청소년기의 사회적응문제에서 긍정적 효과를 줄 수 있는 음악치료 프로그램개발 모형을 위한 기초 자료로 삼고자 한다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권9호
• /
• pp.3817-3825
• /
• 2015

Background: The human protein methyl-transferase DOT1L catalyzes the methylation of histone H3 on lysine 79 (H3K79) at homeobox genes and is also involved in a number of significant processes ranging from gene expression to DNA-damage response and cell cycle progression. Inhibition of DOT1L activity by shRNA or small-molecule inhibitors has been established to prevent proliferation of various MLL-rearranged leukemia cells in vitro, establishing DOT1L an attractive therapeutic target for mixed lineage leukemia (MLL). Most of the drugs currently in use for the MLL treatment are reported to have low efficacy, hence this study focused on various natural compounds which exhibit minimal toxic effects and high efficacy for the target receptor. Materials and Methods: Structures of human protein methyl-transferase DOT1L and natural compound databases were downloaded from various sources. Virtual screening, molecular docking, dynamics simulation and drug likeness studies were performed for those natural compounds to evaluate and analyze their anti-cancer activity. Results: The top five screened compounds possessing good binding affinity were identified as potential high affinity inhibitors against DOT1L's active site. The top ranking molecule amongst the screened ligands had a Glide g-score of -10.940 kcal/mol and Glide e-model score of -86.011 with 5 hydrogen bonds and 12 hydrophobic contacts. This ligand's behaviour also showed consistency during the simulation of protein-ligand complex for 20000 ps, which is indicative of its stability in the receptor pocket. Conclusions: The ligand obtained out of this screening study can be considered as a potential inhibitor for DOT1L and further can be treated as a lead for the drug designing pipeline.

• 만화애니메이션 연구
• /
• 통권40호
• /
• pp.109-137
• /
• 2015

디지털 기술이 발전함에 따라 선으로 많은 것을 표현하던 전통적 제작 방식의 드로잉 애니메이션은 주 소비층인 유소년을 디지털 시네마의 리얼리즘에 빼앗기고 있다. 과거 속도감, 역동성, 박진감 등의 스펙터클로 유소년의 관심을 받던 드로잉 애니메이션은 오랫동안 쇠퇴기를 맞았지만, 디지털 기술을 적절히 혼합하는 효과적인 연출기법을 꾸준히 정립시켜 특유의 역동성과 속도감에 대한 표현기술을 진화시켜오고 있다. 본고는 주로 일본의 리미티드 애니메이션에서 나타나는 움직임의 역동성과 빠른 속도감의 원인을 구체적으로 규명하는데 그 목적이 있다. 속도감과 역동성이 강조되는 장르 중 편중현상이 가장 크다고 생각되는 초인적 힘을 가진 영웅을 등장시키는 몇몇 액션 애니메이션 작품을 통해 디지털기술이 이입되기 전의 연출법과 후의 연출법을 비교 분석해 보았다. 본고는 베르그송과 맥루언의 논의를 각각 지표기호의 개입과 생략기법에 따른 수용자의 참여행위에 대입시켜 결론을 도출한다. 이는 앞으로도 계속될 히어로 물의 성장과 함께 특유의 연출을 통해 제약적인 물리법칙을 거슬러 감각의 확장을 이끌어내는 드로잉 애니메이션의 표현 요소가 무엇인지 탐구해보는데 그 의의를 가진다.

• Molecules and Cells
• /
• 제43권10호
• /
• pp.870-879
• /
• 2020

Dendrites require precise and timely delivery of protein substrates to distal areas to ensure the correct morphology and function of neurons. Many of these protein substrates are supplied in the form of ribonucleoprotein (RNP) complex consisting of RNA-binding proteins (RBPs) and mRNAs, which are subsequently translated in distal dendritic areas. It remains elusive, however, whether key RBPs supply mRNA according to local demands individually or in a coordinated manner. In this study, we investigated how Drosophila sensory neurons respond to the dysregulation of a disease-associated RBP, Ataxin-2 (ATX2), which leads to dendritic defects. We found that ATX2 plays a crucial role in spacing dendritic branches for the optimal dendritic receptive fields in Drosophila class IV dendritic arborization (C4da) neurons, where both expression level and subcellular location of ATX2 contribute significantly to this effect. We showed that translational upregulation through the expression of eukaryotic translation initiation factor 4E (eIF4E) further enhanced the ATX2-induced dendritic phenotypes. Additionally, we found that the expression level of another disease-associated RBP, fragile X mental retardation protein (FMRP), decreased in both cell bodies and dendrites when neurons were faced with aberrant upregulation of ATX2. Finally, we revealed that the PAM2 motif of ATX2, which mediates its interaction with poly(A)-binding protein (PABP), is potentially necessary for the decrease of FMRP in certain neuronal stress conditions. Collectively, our data suggest that dysregulation of RBPs triggers a compensatory regulation of other functionally-overlapping RBPs to minimize RBP dysregulation-associated aberrations that hinder neuronal homeostasis in dendrites.

• Journal of Animal Science and Technology
• /
• 제63권6호
• /
• pp.1376-1385
• /
• 2021

Foot-and-mouth disease, one of the most contagious diseases in cloven-hoofed animals, causes significant economic losses. The pathogenesis of foot-and-mouth disease virus (FMDV) infection is known to differ with age of the animals. In this study, we aimed to reveal the difference in immunological response in the initial stage of FMDV infection between piglets and adult pigs. Peripheral blood mononuclear cells (PBMCs) were isolated from 3 piglets (8 weeks old) and 3 pigs (35 weeks old) that were not vaccinated against FMDV. O-type FMDV (2 × 10² median tissue culture infectious dose) was inoculated into porcine PBMCs and the cells were incubated at 37.0℃ under 5% CO₂ for various time periods (0, 1, 3, 6, 12, 24, and 48 h). The total RNA was obtained from the FMDV-inoculated PBMCs after each time point, and the virus titer was investigated in these RNA samples. Furthermore, dynamics of mRNA expression of the six tested cytokines (interferon [IFN]-α, IFN-γ, interleukin [IL]-6, IL-8, IL-10, and tumor necrosis factor [TNF]-α) in FMDV-inoculated porcine PBMCs were evaluated by time-series analysis to determine the differences, if any, based on the age of the pigs. The PBMCs of piglets contained the highest quantity of FMDV mRNA at 6 hours post-inoculation (hpi), and the PBMCs of pigs had the highest quantity of FMDV mRNA at 3 hpi. The mean cycle threshold-value in the PBMCs steadily decreased after the peak time point in the piglets and pigs (6 and 3 hpi, respectively). The dynamics of mRNA expression of all cytokines except TNF-α showed age-dependent differences in FMDV-inoculated PBMCs. The mRNA expression of most cytokines was more pronounced in the piglets than in the pigs, implying that the immune response against FMDV showed an age-dependent difference in pigs. In conclusion, within 48 hpi, the 8-week-old piglets responded more rapidly and were more sensitive to FMDV infection than the 35-week-old pigs, which could be associated with the difference in the pathogenesis of FMDV infection among the pigs. These results provide valuable insights into the mechanisms underlying the age-dependent differences in immune response in pigs against FMDV infection.

• 대한임상검사과학회지
• /
• 제56권1호
• /
• pp.10-20
• /
• 2024

RNA-시퀀싱은 표본에 대한 전사체 전체의 패턴을 제공하는 기법이다. 그러나 RNA-시퀀싱은 표본 내 전체 세포에 대한 평균 유전자 발현만 제공할 수 있으며, 표본 내의 이질성(heterogeneity)에 대한 정보는 제공하지 못한다. 단일 세포 RNA-시퀀싱 기술의 발전을 통해 우리는 표본의 단일 세포 수준에서 이질성과 유전자 발현의 동역학(dynamics)에 대한 이해를 할 수 있게 되었다. 예를 들어, 우리는 단일 세포 RNA-시퀀싱을 통해 복잡한 조직을 구성하는 다양한 세포 유형을 식별할 수 있으며, 특정 세포 유형의 유전자 발현 변화와 같은 정보를 알 수 있다. 단일 세포 RNA-시퀀싱은 처음 도입된 이후 많은 이들의 관심을 끌게 되었으며, 이를 활용하기 위한 대규모 생물정보학(bioinformatics) 도구가 개발되었다. 그러나 단일 세포 RNA-시퀀싱에서 생성된 빅데이터 분석에는 데이터 전처리에 대한 이해와 전처리 이후 다양한 분석 기술에 대한 이해가 필요하다. 본 종설에서는 단일 세포 RNA-시퀀싱 데이터분석과 관련된 작업과정의 개요를 제시한다. 먼저 데이터의 품질 관리, 정규화 및 차원 감소와 같은 데이터의 전 처리 과정에 대해 설명한다. 그 이후, 가장 일반적으로 사용되는 생물정보학 도구를 활용한 데이터의 후속 분석에 대해 설명한다. 본 종설은 이 분야에 관심이 있는 새로운 연구자를 위한 가이드라인을 제공하는 것을 목표로 한다.

• 한국약용작물학회지
• /
• 제12권2호
• /
• pp.154-162
• /
• 2004

Hairy roots were induced from Korean ginseng (Panax ginseng C. A. Meyer) root explants and studied for their gene expression. A total of 3,000 ESTs (expressed sequence tags) from ginseng hairy root were determined and about 2,700 ESTs have a length of readable sequence, which result in 1,352 unique ESTs sequences. The 879 ESTs showed significant similarities to known nucleotide or amino acid sequences in other plant species, which were divided into eleven categories depending upon gene function. The remaining 473 sequences showed no significant matches, which are likely to be transcripts or to be matched to other organisms. The results indicated that the analysis of the ginseng hairy root ESTs by partial sequencing of random cDNA clones may be an efficient approach to isolate genes that are functional in ginseng root in a large scale. Our extensive EST analysis of genes expressed in ginseng hairy root not only contributes to the understanding of the dynamics of genome expression patterns in root organ but also adds data to the repertoire of all genomic genes.

• 한국의류학회지
• /
• 제36권5호
• /
• pp.489-500
• /
• 2012

This work surveys the trend and dissolution characteristics in headdress that appear in Jean Paul Gaultier's collection. Along with a related literature review, a total of 903 headdress pieces shown in collections 40 times (excluding the common caps and hats) were analyzed, covering from 2001S/S to 2010 F/W of Jean Paul Gaultier's Haute Couture and Pret-a-Porter. The headdress trend indicated in his collections was divided into such subgroup forms of folklore, usage of natural things, usage of artificial things, religion, retro, scarf, variations in headgear, mask and veil, atypical type and abstract. Such an expression tendency was so unique and mixed characteristic that it was hard to define its form and structure thanks to enlargement and exaggeration, extremity in ornaments, and use of foreign materials, which led to creative dynamics. Gaultier's headdress also reflected the following characteristics: first, expression of difference indicative of time deconstruction; second, uncertainty of meanings via deconstruction; third, text interactivity via deconstruction of gender and material adopted; fourth, decentralization through dissolution of the Orient and the Occident.

• 대한가정학회지
• /
• 제44권4호
• /
• pp.1-9
• /
• 2006

This study examines the esthetic characteristics in the haute couture work of Jean Paul Gaultier, who represents the fashion in France and is famous for avant-garde and experimental works through dismantling, from the first haute couture collection in 1997 to the present. The materials for the study are the precedent studies, the related literature, and the photographs of the works and the interview articles in domestic and international fashion journals. Three characteristics are revealed in his works. First of all, he provided a transcendental fashion different from the established wearing or ornamental ways by dismantling the dichotomies between male and female, time and space, and beauty and ugliness. Second, he reflected the decadent beauty recognized as representing women's sexual and provocative expression based on exposure, suppression, perversion, and grotesque manifestations by shaping an esthetic value within a different point of view. Finally, he was characterized as being transcendental with an eclectic fusion of intercultural differences or dynamics, items in costume formation, time and space, and eastern and western. This transcendental expression, Gaultier's desire for creativeness, can be an ideal characterizing this era.