The present study was conducted to evaluate whether vitrification could be used for ovarian tissue preservation. The important issue here is that the vitrification is very simple, easy, and economical compared to the conventional cryopreserving method that using automatic freezing instrument. Human ovarian cortical tissues were cryopreserved by vitrification with 5.5 M ethylene glycol and 1.0 M sucrose as cryoprotectant. Three points of temperature ($4^{\circ}C$, room temperature, and $37^{\circ}C$) and two points of duration (5 or 10 minutes) for cryoprotectant treatment were examined to determine the best condition for vitrification of the human ovarian cortical tissues. After thawing, viability of the isolated primordial follicles was examined by dye-exclusion method. Histological appearance of tissues before and after the cryopreservation was evaluated. There was no toxic effect of the 5.5 M ethylene glycol on the primordial follicles. However, when the tissues were treated with cryoprotectant at $37^{\circ}C$ for 10 minutes and exposed to liquid nitrogen, it seems likely that there is certain deleterious effects on the viability of the primordial follicles. The highest viability of the primordial follicles was obtained with the treatment of cryoprotectant at room temperature for 10 minutes. Follicles and oocytes survived after freezing and thawing had the similar normal shapes as was seen in the specimens before cryopreservation. It would be useful to apply vitrification in establishing ovarian tissue banking for clinical purposes.
This study is designed to assess the priority order of the chemicals to cause to generate occupational diseases in order to understand the fundamental data required for the preparation of health protective measure for the workers dealing with chemicals. The 41 types of 51 ones of chemicals to cause to generate the national occupational diseases were selected as the study objects by understanding their domestic use or not, and their occupational diseases' occurrence or not among 110,608 types of domestic and overseas chemicals. To assess their priority order the sum of scores was acquired by understanding the actually classified condition based on a perfect score of physical riskiness(90points) and health toxicity(92points) as a classification standard by GHS, the priority order on GHS riskiness assessment, GHS toxicity assessment, GHS toxic xriskiness assessment(sum of riskiness plus toxicity) was assessed by multiplying each result by each weight of occupational disease's occurrence. The high ranking 5 items of chemicals for GHS riskiness assessment were turned out to be urethane, copper, chlorine, manganese, and thiomersal by order. Besides as a result of GHS toxicity assessment the top fives were assessed to be aluminum, iron oxide, manganese, copper, and cadium(Metal) by order. On the other hand, GHS toxicity riskiness assessment showed that the top fives were assessed to be copper, urethane, iron oxide, chlorine and phenanthrene by order. As there is no material or many uncertain details for physical riskiness or health toxicity by GHS classification though such materials caused to generate the national occupational diseases, it is very urgent to prepare its countermeasure based on the forementioned in order to protect the workers handling or being exposed to chemicals from health.
Choi, Min-Jung;Chaung, Seung-Kyo;Park, Jin Hee;Chang, Soo Jung;Sohng, Kyeong-Yae
Journal of Korean Academy of Fundamentals of Nursing
/
v.25
no.4
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pp.231-239
/
2018
Purpose: This study was done to understand nursing students' exposure of needlestick and sharp injuries (NSSI), level of anxiety as well as experience of practicing injection with human beings during Fundamentals of Nursing (FN) practice. Methods: Data consisted of self-report questionnaires completed by 571 nursing students who had completed an injection practice in FN. The questionnaire included questions on general characteristics, exposure to NSSI, experience of practicing injections with human beings, level of anxiety and other experiences. Data were analyzed using descriptive statistics and content analysis. Results: Of the students, 17.5% experienced NSSI and 83.2% performed invasive injection procedures in the FN practice. The level of anxiety was 4.62-6.46/10 points when injecting a classmate and 1.27-1.93/10 points when using manikins. The feeling most often reported was worried about making a mistake that could hurt my classmate (57.2%). Conclusion: Nursing students are exposed to NSSI and experience a high degree of anxiety in invasive injection practice. These results indicate that it is necessary to develop and implement standardized NSSI prevention and anxiety reduction programs in FN.
International Journal of Computer Science & Network Security
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v.22
no.5
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pp.368-374
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2022
Children are by nature curious and enthusiastic about learning and love to explore and search for everything they see around them, but as a result of this exploration they may sometimes be exposed to dangerous situations ranging from falls to poisoning and suffocation. That is why when supporting a child's natural desire to explore the world and supporting his awareness of dangerous situations and good handling of them, helps him build a conscious scientific mind and enhance his curiosity in the natural world. It is not easy to imagine a difficult situation in which we or one of our family is in danger, unable to help ourselves or to help them in time, due to our complete ignorance of the rules of first aid. Hence the importance of learning first aid not only for the child but for the community and the world at large. "Hakeem" is an Arabic E-health educational application that aims to teach children from the age of six to eleven years first aid, in our belief that the seed of renaissance lies in the care and education of children, and the lack of Arabic content that aims to teach children first aid skills. The idea is to create a scenario in which the child is responsible for saving the person who will be in a dangerous situation using Augmented Reality (AR) technology, to increase engagement and interaction and provides a rich user experience, and according to the child's performance, he will get reward points. The game will have several levels: Beginner, Intermediate, and Hakeem, and based on the player's points he will get a title and move to the next level, and when he reaches the end, he will get the certificate.
Kim, Seog-ku;Jeong, Jang-sik;Kwak, Phill-jae;Choi, Jun-hyang;Lee, Hyun-dong;Lee, Tae-yoon
Journal of Korean Society on Water Environment
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v.20
no.2
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pp.163-169
/
2004
Feasibility of using PE sheet laminated CSP(Corrugated steel pipe) to sewers were tested via corrosion and abrasion tests for PE sheet laminated CSP; 1) corrosion test for PE film and PE coated plate, 2) corrosion test for joint of PE sheet laminated CSP, 3) corrosion test for outside of PE sheet laminated CSP, and 4) abrasion test for inside of PE sheet laminated CSP. The results for the corrosion and abrasion tests are as follows. 1. Results for corrosion tests on PE films and zinc coated steel plates that were coated with PE films show that the surfaces of both PE were not changed compared to those of original PE samples. Furthermore, PE films maintained strong adhesion on the steel plates even though they were exposed to sewage and seawater provided cutting planes of the steel plates were treated with molding. But, results for corrosion tests on the spots that were previously cut off by a knife and a cutting planes of the steel plates were treated without molding show that steel plates have been corroded if they were exposed to sewage and seawater. 2. Results for corrosion tests on joints of PE sheet laminated corrugated steel using sewage show that gathering rust was observed at cutting plane of PE sheet laminated corrugated steel after 180 days, but PE film was not flaked off. However, PE film at cutting plane was flaked off when PE sheet laminated corrugated steel was exposed to seawater. Furthermore, flaking process was severely progressed at lockseam points of which the surfaces were not smooth. 3. Results for laying PE sheet laminated corrugated steel under the ground show that the surface of untouched PE film was almost identical to that of original PE film. However, the spots that were previously cut off by a knife and a cutting plane of PE sheet laminated corrugated steel have flaked off. 4. As a result of abrasion tests conducted with PE sheet laminated corrugated steel and sand, brilliance and thickness of PE was not greatly changed under the severe abrasion conditions. Therefore, the durability of PE sheet laminated corrugated steel for abrasion was determined to be good.
The satellite sensor model is typically established using ground control points acquired by ground survey Of existing topographic maps. In some cases where the targeted area can't be accessed and the topographic maps are not available, it is difficult to obtain ground control points so that geospatial information could not be obtained from satellite image. The paper presents several satellite sensor models and satellite image decomposition methods for non-accessible area where ground control points can hardly acquired in conventional ways. First, 10 different satellite sensor models, which were extended from collinearity condition equations, were developed and then the behavior of each sensor model was investigated. Secondly, satellite images were decomposed and also pseudo images were generated. The satellite sensor model extended from collinearity equations was represented by the six exterior orientation parameters in 1$^{st}$, 2$^{nd}$ and 3$^{rd}$ order function of satellite image row. Among them, the rotational angle parameters such as $\omega$(omega) and $\phi$(phi) correlated highly with positional parameters could be assigned to constant values. For non-accessible area, satellite images were decomposed, which means that two consecutive images were combined as one image. The combined image consists of one satellite image with ground control points and the other without ground control points. In addition, a pseudo image which is an imaginary image, was prepared from one satellite image with ground control points and the other without ground control points. In other words, the pseudo image is an arbitrary image bridging two consecutive images. For the experiments, SPOT satellite images exposed to the similar area in different pass were used. Conclusively, it was found that 10 different satellite sensor models and 5 different decomposed methods delivered different levels of accuracy. Among them, the satellite camera model with 1$^{st}$ order function of image row for positional orientation parameters and rotational angle parameter of kappa, and constant rotational angle parameter omega and phi provided the best 60m maximum error at check point with pseudo images arrangement.
The purpose of this study was to examine the infection control attitude of dental hygienists and the relationship between their attitude and general characteristics. The subjects in this study were 235 dental hygienists who received local in-service education on April 27, 2008. The survey was conducted by this researcher with questionnaires that included 11 items about general characteristics and 41 about attitude of dental infection. The findings of the study were as follows: 1. The dental hygienists investigated got a mean of 2.96 out of possible three points on hand washing, which was a high score. Those who had never been exposed to the blood of patients scored better than the others who hadn't. As for infection control attitude of protective personal devices, they got 2.01 out of possible three points. Those who were at the age of 26 to 30 and who had ever received infection control education got better scores. 2. Concerning attitude toward surface disinfectant, they got 1.95 out of possible three points. whose age was shorter, whose career was shorter, who were in charge of treatment, who served at general hospitals and who had ever received infection control education got better scores. In regard to attitude to surface disinfection methods, they got 1.83 out of possible three points. Those who had ever received infection control education got better scores. 3. As for equipment disinfection, they got 2.43 out of possible three points. Those who worked at dental hospitals and who had ever received infection control education got better scores. Concerning attitude to disposal of scrapped materials, they got 2.92 out of possible three points, and all the intergroup gaps weren't significant. 4. Overall, the dental hygienists got a mean of 2.08 out of possible three points on dental infection control, and those who worked at dental hospitals and who had ever received infection control education took a better attitude to dental infection control.
It is important to determine the action spectrum of UV-B radiation contained in the sunlight to estimate the risk of skin cancer. We have investigated action spectra for induction of apoptosis and reproductive cell death in L5178Y cells using the Okazaki Large Spectrograph at NIBB. L5178Y cells were exposed to light at different wavelengths in UV-B or UV-A region. Frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation, and by the colony formation assay, respectively. The measured sensitivity spectra for the two end-points were in very good agreement. Sensitivity decreased steeply with increase of wavelength in UV-B region and remains nearly constant in UV-A region. The action spectra were also slightly steeper than that for the minimum erythematic dose (MED), but very similar to the light absorption spectrum of DNA in UV-B region. On the other hand, the spectra for both endpoints were similar to MED spectrum but not DNA spectrum in the UV-A region. Also different time-course and morphological difference of apoptosis were found between UV-B (long time, fragmentation) and UV-A (short time, shrinkage) region. These results suggest that DNA damage induced by UV-B light triggers apoptosis and reproductive cell death, but other damaged targets (membrane, protein and so on) trigger these effects in UV-A region.
It is different from other countries, they limit the possibility of long-distance travel of infectious waste by principles around treatment and recommend to use various techniques. Medical waste generated from the health medical institutions requires more thorough management because it causes potential danger to anybody exposed and also greatly harmful to natural ecosystems. The amendment bill of Wastes Control Act points out growing risk while consigning to remote waste treatment facilities because of the low processing cost and propose area-division as a solution. This study based on literature reviews, comparison of foreign countries and Korean acts. It requires area-division strategies as a suitable alternative considering the domestic situation.
Human perceptional speed is different from its real speed. There is lack of research that the perceptional speed is different from real speed in 2-dimension, because most research of speed perception has concentrated on points and lines. This research investigates the effects of object size on speed perception. In this research, we used 2-D circular objects of the different size, 0.9, 1.8 and $3.6^{\circ}$. The objects moved 9.0, 13.5 and $18.0^{\circ}$ with three different speeds, 6.0, 9.0 and $18.0^{\circ}$/s. Six participants were exposed to the environment with standard scene(size: $1.8^{\circ}$, speed: $9.0^{\circ}$/s and travel distance: $13.5^{\circ}$). After the first scene, another scene in which the object had changed to different sizes, speeds and distances, was shown to the participants. A magnitude estimation method was used to construct a scale of the perceived speed level. The relationship between the perceived and the actual speed level was explained by Stevens's power law that the value was 0.978 with the exponent of 0.992. The size of object had an effect on the speed perception but travel distance was not. The perceptional speed of bigger object was lower than of smaller object. It showed that the degrees of perceptional speed decreased as size of object increased.
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