• Clinical and Experimental Reproductive Medicine
• /
• 제26권2호
• /
• pp.251-256
• /
• 1999

The present study was conducted to evaluate whether vitrification could be used for ovarian tissue preservation. The important issue here is that the vitrification is very simple, easy, and economical compared to the conventional cryopreserving method that using automatic freezing instrument. Human ovarian cortical tissues were cryopreserved by vitrification with 5.5 M ethylene glycol and 1.0 M sucrose as cryoprotectant. Three points of temperature ($4^{\circ}C$, room temperature, and $37^{\circ}C$) and two points of duration (5 or 10 minutes) for cryoprotectant treatment were examined to determine the best condition for vitrification of the human ovarian cortical tissues. After thawing, viability of the isolated primordial follicles was examined by dye-exclusion method. Histological appearance of tissues before and after the cryopreservation was evaluated. There was no toxic effect of the 5.5 M ethylene glycol on the primordial follicles. However, when the tissues were treated with cryoprotectant at $37^{\circ}C$ for 10 minutes and exposed to liquid nitrogen, it seems likely that there is certain deleterious effects on the viability of the primordial follicles. The highest viability of the primordial follicles was obtained with the treatment of cryoprotectant at room temperature for 10 minutes. Follicles and oocytes survived after freezing and thawing had the similar normal shapes as was seen in the specimens before cryopreservation. It would be useful to apply vitrification in establishing ovarian tissue banking for clinical purposes.

• 한국환경과학회지
• /
• 제19권6호
• /
• pp.715-735
• /
• 2010

This study is designed to assess the priority order of the chemicals to cause to generate occupational diseases in order to understand the fundamental data required for the preparation of health protective measure for the workers dealing with chemicals. The 41 types of 51 ones of chemicals to cause to generate the national occupational diseases were selected as the study objects by understanding their domestic use or not, and their occupational diseases' occurrence or not among 110,608 types of domestic and overseas chemicals. To assess their priority order the sum of scores was acquired by understanding the actually classified condition based on a perfect score of physical riskiness(90points) and health toxicity(92points) as a classification standard by GHS, the priority order on GHS riskiness assessment, GHS toxicity assessment, GHS toxic xriskiness assessment(sum of riskiness plus toxicity) was assessed by multiplying each result by each weight of occupational disease's occurrence. The high ranking 5 items of chemicals for GHS riskiness assessment were turned out to be urethane, copper, chlorine, manganese, and thiomersal by order. Besides as a result of GHS toxicity assessment the top fives were assessed to be aluminum, iron oxide, manganese, copper, and cadium(Metal) by order. On the other hand, GHS toxicity riskiness assessment showed that the top fives were assessed to be copper, urethane, iron oxide, chlorine and phenanthrene by order. As there is no material or many uncertain details for physical riskiness or health toxicity by GHS classification though such materials caused to generate the national occupational diseases, it is very urgent to prepare its countermeasure based on the forementioned in order to protect the workers handling or being exposed to chemicals from health.

• 기본간호학회지
• /
• 제25권4호
• /
• pp.231-239
• /
• 2018

Purpose: This study was done to understand nursing students' exposure of needlestick and sharp injuries (NSSI), level of anxiety as well as experience of practicing injection with human beings during Fundamentals of Nursing (FN) practice. Methods: Data consisted of self-report questionnaires completed by 571 nursing students who had completed an injection practice in FN. The questionnaire included questions on general characteristics, exposure to NSSI, experience of practicing injections with human beings, level of anxiety and other experiences. Data were analyzed using descriptive statistics and content analysis. Results: Of the students, 17.5% experienced NSSI and 83.2% performed invasive injection procedures in the FN practice. The level of anxiety was 4.62-6.46/10 points when injecting a classmate and 1.27-1.93/10 points when using manikins. The feeling most often reported was worried about making a mistake that could hurt my classmate (57.2%). Conclusion: Nursing students are exposed to NSSI and experience a high degree of anxiety in invasive injection practice. These results indicate that it is necessary to develop and implement standardized NSSI prevention and anxiety reduction programs in FN.

• International Journal of Computer Science & Network Security
• /
• 제22권5호
• /
• pp.368-374
• /
• 2022

Children are by nature curious and enthusiastic about learning and love to explore and search for everything they see around them, but as a result of this exploration they may sometimes be exposed to dangerous situations ranging from falls to poisoning and suffocation. That is why when supporting a child's natural desire to explore the world and supporting his awareness of dangerous situations and good handling of them, helps him build a conscious scientific mind and enhance his curiosity in the natural world. It is not easy to imagine a difficult situation in which we or one of our family is in danger, unable to help ourselves or to help them in time, due to our complete ignorance of the rules of first aid. Hence the importance of learning first aid not only for the child but for the community and the world at large. "Hakeem" is an Arabic E-health educational application that aims to teach children from the age of six to eleven years first aid, in our belief that the seed of renaissance lies in the care and education of children, and the lack of Arabic content that aims to teach children first aid skills. The idea is to create a scenario in which the child is responsible for saving the person who will be in a dangerous situation using Augmented Reality (AR) technology, to increase engagement and interaction and provides a rich user experience, and according to the child's performance, he will get reward points. The game will have several levels: Beginner, Intermediate, and Hakeem, and based on the player's points he will get a title and move to the next level, and when he reaches the end, he will get the certificate.

• 한국물환경학회지
• /
• 제20권2호
• /
• pp.163-169
• /
• 2004

Feasibility of using PE sheet laminated CSP(Corrugated steel pipe) to sewers were tested via corrosion and abrasion tests for PE sheet laminated CSP; 1) corrosion test for PE film and PE coated plate, 2) corrosion test for joint of PE sheet laminated CSP, 3) corrosion test for outside of PE sheet laminated CSP, and 4) abrasion test for inside of PE sheet laminated CSP. The results for the corrosion and abrasion tests are as follows. 1. Results for corrosion tests on PE films and zinc coated steel plates that were coated with PE films show that the surfaces of both PE were not changed compared to those of original PE samples. Furthermore, PE films maintained strong adhesion on the steel plates even though they were exposed to sewage and seawater provided cutting planes of the steel plates were treated with molding. But, results for corrosion tests on the spots that were previously cut off by a knife and a cutting planes of the steel plates were treated without molding show that steel plates have been corroded if they were exposed to sewage and seawater. 2. Results for corrosion tests on joints of PE sheet laminated corrugated steel using sewage show that gathering rust was observed at cutting plane of PE sheet laminated corrugated steel after 180 days, but PE film was not flaked off. However, PE film at cutting plane was flaked off when PE sheet laminated corrugated steel was exposed to seawater. Furthermore, flaking process was severely progressed at lockseam points of which the surfaces were not smooth. 3. Results for laying PE sheet laminated corrugated steel under the ground show that the surface of untouched PE film was almost identical to that of original PE film. However, the spots that were previously cut off by a knife and a cutting plane of PE sheet laminated corrugated steel have flaked off. 4. As a result of abrasion tests conducted with PE sheet laminated corrugated steel and sand, brilliance and thickness of PE was not greatly changed under the severe abrasion conditions. Therefore, the durability of PE sheet laminated corrugated steel for abrasion was determined to be good.

• 대한원격탐사학회:학술대회논문집
• /
• 대한원격탐사학회 2001년도 춘계 학술대회 논문집 통권 4호 Proceedings of the 2001 KSRS Spring Meeting
• /
• pp.140-148
• /
• 2001

일반적으로 위성영상의 센서에 대한 모델은 촬영대상지역의 지상기준점을 이용하여 결정하며, 이와 같은 지상기준점은 기존 지형도를 이용하거나 또는 지상측량에 의하여 획득한다. 그러나 지구관측위성에서 얻어진 영상의 촬영지역이 비접근 지역인 경우에는 지상측량에 의하여 지상기준점을 획득하기 어려우며, 또한 대상지역의 지형도가 제작되어 있지 않은 경우에는 실질적으로 위성영상을 이용하여 지형정보를 추출하기 어려운 실정이다. 본 논문은 지상기준점의 취득이 어려운 비접근 지역에 대한 위성영상에서 지형정보를 획득하기 위한 방법을 제시하였다. 먼저, 공선조건식을 기반으로 10개의 위성센서모델을 개발하고, 개발된센서모델의 거동을 분석하기 위하여 Space Resection 및 Space Inetersection을 통해 각각의 센서모델에 대한 적합성을 실험하였다. 이를 바탕으로 비접근 지역에 대한 지형정보를 취득하기 위하여 영상을 재구성하거나 Pseudo 영상을 제작하고, 이에 대한 센서모델의 거동 및 정확도를 분석.제시하였다. 공선조건식을 이용한 Pushbroom 위성영상의 센서모델은 투영 중심의 위치와 회전요소에 대한 6개의 외부표정요소와 상관도가 높은 회전요소($\omega$, $\phi$)를 고정된 값으로 사용하는 센서모델을 개발하였다. 비접근 지역의 영상으로부터 Pseudo 영상을 임의로 제작하여 패스내에서 중복영역을 갖도록 구성하였다. 본 연구에서 이용한 인공위성 데이터는 서로 다른 패스에서 동일한 지역을 촬영한 SPOT 영상이며, 각각의 패스에서 두 장의 연속된 영상을 이용하였다. 개발된 10개의 센서모델과 5가지의 영상 재구성 방법에 따라 비접근 지역에서의 정확도는 다르게 나타났으며, 그 중 투영중심의 위치 및 회전요소 k를 1차 함수로 표현하고 회전요소 $\omega$, $\phi$를 고정시킨 센서모델과 Pseudo 영상을 이용한 방법이 비접근 지역 60km 지점의 검사점에서 최대오차 60m의 결과를 보였다.

• 치위생과학회지
• /
• 제9권1호
• /
• pp.129-136
• /
• 2009

본 연구는 2008년 4월 27일 지역 보수교육에 참석한 치과위생사 235명을 대상으로 치과위생사의 병원감염방지에 대한 태도를 파악하고 연구대상자의 일반적 특성과 연관성을 분석하기 위하여 자기기입식 설문지법을 실시하였으며, 조사항목으로는 일반적 특성 11문항과 치과감염에 대한 태도를 묻는 문항 35문항으로 구성하여 다음같은 결론을 얻었다. 1. 치과위생사의 손 씻기에 대한 태도는 3점 만점 중 2.96으로 높은 것으로 나타났으며, 환자의 혈액에 노출된 경험이 없는 치과위생사가 높았다(p < .05). 개인보호 장비에 대한 태도는 2.01로 나타났으며, 연령이 26~30세인 치과위생사(p < .01)와 감염방지 교육경험이 있는 치과위생사가 높았다(p < .01). 2. 표면소독제에 대한 태도는 1.95로 나타났으며, 연령(p < .001)과 근무경력이 적을수록(p < .01), 진료업무 담당치위생사(p < .001), 종합병원에 근무하는 치과위생사(p < .001), 그리고 감염방지 교육경험이 있는 치과위생사가 높았다(p < .05). 표면소독 방법에 대한 태도는 1.83으로 나타났으며, 감염방지 교육경험이 있는 치과위생사가 높았다(p < .01). 3. 기구관리에 대한 태도는 2.43으로 나타났으며, 치과병원에 근무하는 치과위생사(p < .01)와 감염방지 교육경험이 있는 치과위생사가 높았다(p < .001). 폐기물 관리에 대한 태도는 2.92로 높은 것으로 나타났으며 모든 영역에서 유의한 차이를 보이지 않았다. 4. 치과위생사들의 치과 감염방지에 대한 태도는 전체평균이 2.08로 나타났으며, 치과병원에 근무하는 치과위생사(p < .05)와 감염방지 교육경험이 있는 치과위생사가 치과 감염에 대한 태도가 높았다(p < .001).

• Journal of Photoscience
• /
• 제9권2호
• /
• pp.454-456
• /
• 2002

It is important to determine the action spectrum of UV-B radiation contained in the sunlight to estimate the risk of skin cancer. We have investigated action spectra for induction of apoptosis and reproductive cell death in L5178Y cells using the Okazaki Large Spectrograph at NIBB. L5178Y cells were exposed to light at different wavelengths in UV-B or UV-A region. Frequencies of apoptosis induction and reproductive cell death were determined by counting cells with chromatin condensation, and by the colony formation assay, respectively. The measured sensitivity spectra for the two end-points were in very good agreement. Sensitivity decreased steeply with increase of wavelength in UV-B region and remains nearly constant in UV-A region. The action spectra were also slightly steeper than that for the minimum erythematic dose (MED), but very similar to the light absorption spectrum of DNA in UV-B region. On the other hand, the spectra for both endpoints were similar to MED spectrum but not DNA spectrum in the UV-A region. Also different time-course and morphological difference of apoptosis were found between UV-B (long time, fragmentation) and UV-A (short time, shrinkage) region. These results suggest that DNA damage induced by UV-B light triggers apoptosis and reproductive cell death, but other damaged targets (membrane, protein and so on) trigger these effects in UV-A region.

• 디지털융복합연구
• /
• 제12권9호
• /
• pp.255-263
• /
• 2014

선진국과 달리 한국에서는 낮은 처리비용 등을 이유로 원거리에 있는 폐기물 처리시설에 의료폐기물 처리를 위탁하는 것이 가능하다. 이 과정에서 위험성 때문에 의원입법으로 개정법률안이 국회에 제출되어 있다. 본 연구는 선진국과의 비교연구를 통하여 의료폐기물 관련 문제해결을 위한 효과적인 방안을 모색하는데 그 목적이 있다. 연구방법은 문헌고찰 및 법률의 비교연구를 통한 서술식 방법을 채택하였다. 외국에서는 감염성 폐기물에 대하여 발생지 인근에서 신속한 처리를 원칙으로 하면서 장거리 이동 가능성을 제한하고 다양한 기술사용을 권장하고 있는 것에서 한국과 차이를 보인다. 따라서 외국 사례와 비교하여 의료폐기물 최적의 처리방안으로 권역화 방안을 제시했다.

• 대한인간공학회지
• /
• 제24권2호
• /
• pp.51-56
• /
• 2005

Human perceptional speed is different from its real speed. There is lack of research that the perceptional speed is different from real speed in 2-dimension, because most research of speed perception has concentrated on points and lines. This research investigates the effects of object size on speed perception. In this research, we used 2-D circular objects of the different size, 0.9, 1.8 and $3.6^{\circ}$. The objects moved 9.0, 13.5 and $18.0^{\circ}$ with three different speeds, 6.0, 9.0 and $18.0^{\circ}$/s. Six participants were exposed to the environment with standard scene(size: $1.8^{\circ}$, speed: $9.0^{\circ}$/s and travel distance: $13.5^{\circ}$). After the first scene, another scene in which the object had changed to different sizes, speeds and distances, was shown to the participants. A magnitude estimation method was used to construct a scale of the perceived speed level. The relationship between the perceived and the actual speed level was explained by Stevens's power law that the value was 0.978 with the exponent of 0.992. The size of object had an effect on the speed perception but travel distance was not. The perceptional speed of bigger object was lower than of smaller object. It showed that the degrees of perceptional speed decreased as size of object increased.