• Journal of Life Science
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• v.11 no.2
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• pp.111-115
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• 2001

This study was designed to clone the SNF2/SW12 helicase-related genes from the fission yeast Schizosaccha-romyces pombe and thereafter to elucidate the common functions of the proteins in this family. The $hrp^{2+}$gene was cloned by polymerase chain reaction amplification using degenerative primers from conserved SNF2 motifs within the ERCC6 gene, which encodes a protein involved in DNA excision repair. Like other SNF2/SW12 family proteins, the deduced amino acid sequence of Hrp2 contains DNA-dependent ATPase/7 helicase domains as well as the chromodomain and the DNA binding domain. This configuration is similar to that of mCHD1 (mouse chromo-ATPase/helicase-DNA-dinding protein 1), suggesting that Hrp2 is a S. pombe homolog of mCHD1, which is thought to function in altering the chromatin structure to control the gene expression. To characterize the function of Hrp2, 4 Uracil-Hrp2 fusion protein, it was purified near homogeneity by affinity chromatography on $Ni^{2+}$-NTA agarose, DEAE-Sepharose ion exchange arid Sephacryl S-200 gel filtration chromatographies. The purified fusion protein exhibited DNA-dependent ATPase activity, which was stimulated by both double-stranded and single-stranded DNA. To determine the steady-state level of $hrp^{2+}$ transcripts during growth, cells were cultured in medium and collected at every 2hr to prepare total RNAs. The northern blot analysis showed that the level of $hrp^{2+}$ transcripts reached its maximum before the cells entered the exponential growth phase and then decreased gradually, This result implies that Hrp2 may be required at early stages of cell growth.h.

• Knowledge Management Research
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• v.24 no.3
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• pp.127-148
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• 2023

Traditional Business Intelligence environment is limited to support the rapidly changing businesses and the exponential growth of data in both volume and complexity of data. Companies should shift their business intelligence environment into Self-Service Business Intelligence (SSBI) environment in order to make smarter and faster decisions. However, firms seem to face various challenges in implementing and leveraging the effective business intelligence system, and academics do not provide sufficient studies related including the success factors of SSBI. This study analyzes the three cases of Korean companies in depth, their development process and the assessment of business intelligence, based on the theoretical model on the key success factors of business intelligence systems. The comparative analysis of the three cases including project managers' interviews and performance evaluations provide rich implications for the successful adoption and the use of business intelligence systems of firms. The study is expected to provide useful references for firms to fully leverage the effects of business intelligence systems and upgrade towards self-service business intelligence systems.

• The Journal of the Acoustical Society of Korea
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• v.27 no.6
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• pp.271-278
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• 2008

The electro-mechanical characteristics were theoretically analyzed for the wideband ultrasonic transducer made of non-uniform thickness piezoelectric vibrator. This paper proposes a combination of exponential functions which describes the thickness variation along the length of the vibrator to derive the input admittance and power transfer function of the transducer. The bandwidth and the power transfer function of the transducer were investigated while the lateral shape of the vibrator changes. The results showed there is an optimum shape for the wideband characteristics of the transducer, and the bandwidth has increased up to over 100% as the ratio of minimum value of thickness to maximum value decreases. However, the power transfer function had a downward trend as the ratio of thickness decreases. Also we confirmed that even though the value of transfer function increases as the length of the piezoelectric vibrator increases, the shape providing wideband characteristics is very limited. It means that precision processing is required to manufacturing a wideband ultrasonic transducer with high efficiency.

• Korean Journal of Radiology
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• v.23 no.8
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• pp.835-845
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• 2022

Objective: To analyze the characteristics and trends of scientific publications on thyroid ultrasound (US) from 2001 to 2020, specifically examining the differences among disciplines. Materials and Methods: The MEDLINE database was searched for scientific articles on thyroid US published between 2001 and 2020 using the PubMed online service. The evaluated parameters included year of publication, type of document, topic, funding, first author's specialty, journal name, subject category, impact factor, and quartile ranking of the publishing journal, country, and language. Relationships between the first author's specialty (radiology, internal medicine, surgery, otorhinolaryngology, and miscellaneous) and other parameters were analyzed. Results: A total of 2917 thyroid US publications were published between 2001 and 2020, which followed an exponential growth pattern, with an annual growth rate of 11.6%. Radiology produced the most publications (n = 1290, 44.2%), followed by internal medicine (n = 716, 24.5%), surgery (n = 409, 14.0%), and otorhinolaryngology (n = 171, 5.9%). Otorhinolaryngology and internal medicine published significantly more case reports than radiology (p < 0.001, each). Radiology published a significantly higher proportion of publications on imaging diagnosis (p < 0.001 for all) and a significantly lower proportion of publications on biopsy (p < 0.001 for all) than the other disciplines. Publications produced by radiology authors were less frequently published in Q1 journals than those from other disciplines (p < 0.005 for internal medicine and miscellaneous disciplines and < 0.01 for surgery and otorhinolaryngology). China contributed the greatest number of publications (n = 622, 21.3%), followed by South Korea (n = 478, 16.4%) and the United States (n = 468, 16.0%). Conclusion: Radiology produced the most publications for thyroid US than any other discipline. Radiology authors published more notably on imaging diagnosis compared to other topics and in journals with lower impact factors compared to authors in other disciplines.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.289-295
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• 2024

We have developed an aptamer that specifically binds to Porphyromonas gingivalis to reduce the cellular damage caused by P. gingivalis infection and applied it as a biosensor. P. gingivalis is one of the major pathogens causing destructive periodontal disease among the periodontal microorganisms constituting complex biofilms. Porphyromonas gingivalis G-protein (PGP) known to play an important role in the transmission of germs was used as a target protein for the screening of aptamer. The aptamer that has binds to the G-protein of P. gingivalis, was screened and developed through the Systemic Evolution of Ligands by Exponential Energy (SELEX) method. Modified-Western blot analysis was performed with the aptamer which consisted of 38 single-stranded DNA to confirm the selectivity. ELONA (enzyme linked oligonucleotide assay) used to confirm that the aptamer was sensitive to PGP even at low concentration of 1 ㎍/ml. For the rapid detection of P. gingivalis, we constructed a surface plasmon resonance biosensor with SPREETA using the PGP aptamer. It was confirmed that PGP could be detected as low concentration as at 0.1 pM, which is the minimum concentration of aptamer sensor within 5 min. Based on these results, we have constructed a SPREETA biosensor based on aptamer that can bind to P. gingivalis G-protein. It can be used as an infection diagnosis system to rapidly diagnose and analyze oral diseases caused by P. gingivalis.

• The Journal of the Petrological Society of Korea
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• v.26 no.1
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• pp.45-54
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• 2017

The characteristics of the rock cleavage in Jurassic Geochang granite were analysed using the distribution of microcrack lengths and spacings. The phases of distribution of the above lengths and spacings were derived from the enlarged photomicrographs(${\times}6.7$) of the thin section. First, the length and spacing-cumulative diagrams for the six directions of rock cleavages were arranged in increasing order($H2{\rightarrow}R1$) on the density(${\rho}$) of microcrack length. The various parameters were extracted through the combination of the above two types of diagrams. The discrimination factors representing the three quarrying planes and three rock cleavages were acquired through the mutual contrast between the values of parameters. The analysis results of the research are summarized as follows. The evaluation for the six directions of rock cleavages was performed using the parameters such as (1) intersection angle(${\alpha}-{\beta}$) and (2) exponent difference(${\lambda}_S-{\lambda}_L$) between two exponential straight lines related to spacing(${\alpha}$, ${\lambda}_S$) and length(${\beta}$, ${\lambda}_L$). The values of parameters(1 and 2) are in order of H(hardway, (H1 + H2)/2) < G(grain, (G1 + G2)/2) < R(rift, (R1 + R2)/2). On the contrary, the values of the above two parameters for three planes are in order of R < G < H. Meanwhile, the direction of convergence between two exponential straight lines was derived. The above direction is compliant to arrangement of the line os' centering around the line ol. The above two lines converge in the direction of the Y-axis when the line ol and line os' occupy the upper region on the left and the lower region on the right, respectively(R-type). On the contrary, the above two lines converge in the direction of the X-axis when the order of arrangement between line ol and line os' is reversed(H-type). Especially, the positive(+) or negative(-) value of intersection angle(${\alpha}-{\beta}$) is determined by the arrangement of two vertical lines. This type of correlation analysis is useful for evaluating the relative strength of rock cleavage and discriminating three quarrying planes.

• Korean Journal of Soil Science and Fertilizer
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• v.39 no.5
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• pp.259-267
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• 2006

Hydraulic conductivity is the rate of water flux on hydraulic gradient. The van Genuchten Mualem (VGM) model is frequently used for describing unsaturated state of soils, that is composed with the function of soil water potential and soil water content and requests various parameters. This study is to get the value of VGM parameters used Rosetta computer program based on neural network analysis method and to calculate VGM parameters. VGM parameters included Ko(effective saturated hydraulic conductivity), ${\theta}r$(residual soil water content), ${\theta}s$(saturated soil water content), L, n and m. The unsaturated hydraulic conductivity at 10 kPa was calculated by using Rosetta program. Unsaturated hydraulic conductivities of 17 soil series at 1, 3, 5, 7 kPa were also obtained by applying saturated hydraulic conductivity by disk tension infiltrometer based on Gardner and Wooding's equation. Water flow at the water potential of 3 kPa was very low except Namgye, Hagog, Baegsan, Sangju, Seogcheon, Yesan soil series. Unsaturated hydraulic conductivity at 1 kPa showed the highest value for Samgag soil series and was in order of Yesan, Hwabong, Hagog and Baegsan soil series. Those of Gacheon, Seocheon and Ugog soil series were very low. When the value by VGM was compared with the value by disc tension infiltrometer, there was a tendency with exponential function to soils without gravel but there was no tendency to soils including gravel. Conclusively, it would be limited that VGM model for unsaturated hydraulic conductivity analysis applies to Korean agricultural land including gravel and having steep slope, shallow soil depth.

• Journal of Korean Society of Transportation
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• v.26 no.3
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• pp.41-51
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• 2008

As the local bus service diagram after the bus route reform is studied by variable analysis based on traffic card data and income adjustment data, the characteristic of the local bus system is revealed in Busan Metropolitan city. The relationship between traveling length and traveling time is influenced by traveling velocity. In order to keep a headway within 10 minutes, bus service number per minute should be over 0.1013 vehicles. The traveling time of afternoon is generally longer than that of forenoon. Compared with the bus used by a lot people, the deviation of that used by a few people is larger in the all cases of length, headway, time and velocity. According to the analysis of the relationship among card trip number, average income and transfer rate, the relationship between card trip number and average income is expressed as linear function in the general bus and as exponential function in the high-grade & rapid bus. The 1% increase of transfer rate is equal to 6.3 trip/vehicle/day decrease and 4.9 trip/vehicle/day decrease in two bus types respectively. The four effective variables are defined by the discriminant analysis between the profitable routes and the unprofitable; According to discriminant size, bus service number per km, bus via suburb, subway meeting number, bus via university. In order to increase the income when the minibus will be included among public transit transfer system in 2008, it should be necessary to settle the bus network and revitalize the public transit better. In order to decrease the cost, it should be necessary to reorganize the hierarchy between the local bus and the minibus better.

• Composites Research
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• v.30 no.5
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• pp.323-330
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• 2017

In this study, multiscale analysis in which the information obtained from molecular dynamics simulation is applied to the continuum mechanics level is conducted to investigate the effects of clustering of silicon carbide nanoparticles reinforced into polypropylene matrix on mechanical behavior of nanocomposites. The elastic behavior of polymer nanocomposites is observed for various states of nanoparticulate agglomeration according to the model reflecting the degradation of interphase properties. In addition, factors which mainly affect the mechanical behavior of the nanocomposites are identified, and new index 'clustering density' is defined. The correlation between the clustering density and the elastic modulus of nanocomposites is understood. As the clustering density increases, the interfacial effect decreased and finally the improvement of mechanical properties is suppressed. By considering the random distribution of the nanoparticles, the range of elastic modulus of nanocomposites for same value of clustering density can be investigated. The correlation can be expressed in the form of exponential function, and the mechanical behavior of the polymer nanocomposites can be effectively predicted by using the nanoparticulate clustering density.

• Journal of the Korea Concrete Institute
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• v.21 no.1
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• pp.13-20
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• 2009

The influence of steel fiber volume on the tension softening behavior in steel fiber-reinforced ultra high strength concrete was investigated. Three-point bending test (TPBT) with notched beams was performed and inverse analysis method by Uchida et al. was adopted to obtain the tension softening behaviors from the results of TPBT. It could be found that the intial stiffness was constant regardless of steel fiber volume, the increase of steel fiber volume fraction made the tensile strength higher, but all of the curves converged on an asymptote with a crack width. It was proposed the equation of softening curve expressed by combination of plastic behavior part and exponential descending behavior part considering the steel fiber volume fraction and $\omega_0$, which is corresponding to the maximum crack width of plastic area. Thereafter, the crack propagation analysis using finite element method with smeared crack model was also carried out and it was confirmed that the proposed equation had a good agreement with the experimental results.