• 제목/요약/키워드: experimental test

검색결과 18,575건 처리시간 0.057초

체온계투입 부위에 따른 구강체온 차이에 관한 실험적 연구 (An Experimental Study on Body Temperature Differ Differences Taken in Different Areas in the Mouth - Comparison of Body Temperature between Right and Left Subligual Areas According to Time Intervals -)

  • 임난영
    • 대한간호학회지
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    • 제7권1호
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    • pp.63-72
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    • 1977
  • The Purposes of this study are to determine differences of body temperature between Right arid left subligual areas itself and differences depending upon the utilization rate of mastication according to time intervals and to determine the length of time necessary for temperature taking. This Experiment was conducted from Oct 6 through Oct 11, 1975. in which accurately tested clinical Centigrade Thermometers have been utilized. Two thermometers were inserted simultaneously under the right and left sublingual areas and the mouth kept closed while thermometers were in Place. Temperature readings were dr no at three minutes, five minutes and ten minutes. These procedures were repeated one hundred times to different subjects and the data were analyzed statistically by means of the t-test and the F-ratio. Under the 10 hypotheses designed for this study, The findings obtained are as follows; 1. The body temperatures taken at 3, 5, 10minutes intervals in the left sublingual areas were significantly higher than in the right sublingual areas , The average differences of body temperature between the right and left sublingual areas were 0.09$^{\circ}C$, 0.05$^{\circ}C$ and 0.03$^{\circ}C$ in the oder of time interval of 3, 5, and 10 minutes. 2. The body temperatures taken in the right sublingual areas among three different temperature readings, 3, 5 and 10 minutes were significantly different in 57 subjects who have been utilizing evenly both sides of the Teeth. The average readings in a group taking for 3 minutes was 37.04$^{\circ}C$, for 5 minutes 37.15$^{\circ}C$ and for 10minutes 37.28$^{\circ}C$. 3. The body temperatures taken in the left sublingual areas among three different temperature readings, 3, 5 and 10 minutes were significantly different in 57 subjects who have been utilizing evenly both sides of the tenth. The average reading in a group taking for 3 minutes was 37.13$^{\circ}C$, for 5 minutes 37.2$^{\circ}C$ and for 10 minutes 37.31$^{\circ}C$. 4., Oral temperatures taken at 3, 5, 10 minutes intervals at the side of mouth utilized for more frequent mastication were Significantly higher than the other side. The average differences of body temperature between more frequently utilized side and Less frequently utilized side were 0.08f, 0.08f and 0.09f in the order of time interval of 3, 5 and 10 minutes. 5. Oral temperature taken at the side of mouth more frequently utilized for mastication among three different temperature readings, 3, 5 and 10 minutes were significantly different in 43 subjects who have been unequally utilizing either side of teeth. The average reading in a group taking for 3 minutes was 37.09$^{\circ}C$, for 5 minutes 37.17$^{\circ}C$ and for 10 minutes 37.3$^{\circ}C$. 6. Oral temperature taken at the side of mouth less frequently utilized for mastication among three different temperature readings 3, 5 and 10 minutes were significantly different in 43 subjects who have been unequally utilizing either side of teeth. The average reading in a group taking for 3 minutes was 37.01$^{\circ}C$, for S minutes 37.09$^{\circ}C$ and for minutes 37.21$^{\circ}C$. As a result of this study, these differences among time intervals were statistically significant, but there were not so much differences as to be considered important in the clinical practice. Therefore, there would be clinically little difference between two groups who are taking for 3 minutes and for 10 minutes.

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GABA 생성능 우수 김치 젖산균 Lactobacillus sp. OPK2-59의 간 기능 개선 효과 (Effects of Kimchi Lactic Acid Bacteria Lactobacillus sp. OPK2-59 with High GABA Producing Capacity on Liver Function Improvement)

  • 배미옥;김혜진;차연수;이명기;오석흥
    • 한국식품영양과학회지
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    • 제38권11호
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    • pp.1499-1505
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    • 2009
  • 본 연구는 김치에서 분리한 GABA 생성능이 우수한 젖산균을 이용하여 알코올 투여 흰쥐의 간 기능 개선에 미치는 영향을 조사하였다. 실험동물의 체중 및 식이섭취량, 혈청 및 간 지질농도, 간 질환과 관련된 혈청 및 간 효소 활성, 혈중 에탄올 농도, 항산화 효소 활성을 측정한 결과는 다음과 같다. 체중 및 식이섭취량은 각 그룹간의 유의적인 차이를 나타내지 않았으며 각 기관 무게도 군 간의 유의적인 차이를 나타내지 않았으나 정상식이 섭취 군에 비해 알코올 투여 군에서 체중과 식이섭취량이 약간 감소하는 경향을 보였다. 알코올 섭취로 인해 높아진 혈중 총 콜레스테롤 및 중성지질은 젖산균 GABA 파우더투여 군에서 유의적인 차이를 보이며 정상식이 군과 비슷한 수준으로 낮아졌다. 또한 HDL-cholesterol은 군 간의 차이를 보이지는 않았으나, 알코올투여 군에 비해 높아지는 경향을 보였다. 간의 총콜레스테롤 농도는 군 간의 유의적인 차이는 없었으나, 중성지질 농도는 실험군 간의 현저한 차이를 보이며 알코올 투여 군에 비해 젖산균 GABA 파우더 투여 군에서 정상식이 군과 비슷한 수준으로 낮아졌다. 혈중 효소활성 GOT, GPT는 알코올투여 군에 비해 젖산균 GABA 파우더투여 군에서 유의적인 차이를 보이며 정상수준으로 낮아졌으며, 간 GOT는 젖산균 GABA 파우더투여로 인해 현저한 차이를 보이며 알코올투여 군에 비해 낮아졌다. GPT는 각 그룹 간 유의적인 차이를 보이지 않았다. 혈중 알코올 농도를 시간별(0분, 30분, 60분, 120분, 240분, 360분)로 측정한 결과 그룹간의 유의적인 차이를 나타내지 않았으나 젖산균 파우더투여 군에서 알코올 대사를 촉진하는 경향을 보였다. 각 그룹간의 간 조직 중 SOD 활성은 알코올투여 군에 비해 젖산균 파우더투여 군과 젖산균 GABA 파우더 투여 군에서 유의적으로 높은 SOD 수준을 나타내었다. 본 실험을 통해 김치에서 분리한 젖산균 OPK2-59 GABA 파우더가 혈중 총 콜레스테롤과 중성지질, 간의 중성지질 농도를 유의적으로 감소시키고, 혈중 GOT, GPT, 간 GOT 효소활성 감소, 알코올대사 촉진, 항산화 효소활성을 상승시키는 효과를 확인하였다. 이는 김치유래의 젖산균 OPK2-59 GABA 파우더가 알코올투여 흰쥐의 지방대사 및 간 기능을 개선시키는데 기여할 수 있음을 보여주는 것으로, 향후 이러한 효능에 대한 심도 있는 기작연구와 더불어 다른 효능에 대한 연구가 기대된다. 또한 본 젖산균을 김치발효용 스타터로 활용하여 기능성식품을 개발하게 된다면 우수한 전통생물자원을 이용하여 국민건강 증진에 크게 기여할 수 있을 것으로 사료된다.

Digital Imaging Fiber-Optic Trans-Illumination과 Laser Fluorescence를 이용한 법랑질 우식증의 조기 진단에 관한 비교 연구 (COMPARATIVE STUDY ON THE EARLY DETECTION OF ENAMEL LESIONS USING DIFOTI AND LASER FLUORESCENCE)

  • 맹명호;김승오;김종수
    • 대한소아치과학회지
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    • 제33권2호
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    • pp.207-220
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    • 2006
  • 새로 개발된 조기 진단 장비들로는 laser fluorescence device(LFD), 초음파 진단 시스템, confocal laser scanning microscopy(CLSM), quantitative light-induced fluorescence(QLF) 시스템 그리고 digital imaging fiber-optic trans-illumination(DIFOTI) 시스템 등이 있다. 본 연구는 임상에서 사용되고 있는 DIFOTI시스템과 LFD를 이용하여 유치 교환 시기에 있는 환아 21명, 25개의 유치를 대상으로 각 치아당 $1{\sim}3$점을 선정하여 구강내에서 초기 우식법랑질에 대한 DIFOTI 이미지 촬영 결과와 LFD 계측값을 3회 측정하고 그 평균을 대표값으로 한 결과를 CLSM과 비교하여 진단 능력을 평가하였다. 실험실 연구에서는 인공우식 용액을 이용하여 수거된 40개의 유치를 협설면에 $2{\times}3mm$ 크기의 창을 형성하고 4, 8, 12, 16일간 탈회시키면서 그 변화를 DIFOTI 시스템과 LFD를 이용하여 측정하고 이를 CLSM과 비교 분석하여 다음과 같은 결론을 얻었다. 1. 구강내에서 촬영된 DIFOTI의 민감도는 88.2%이었고, 특이도는 76.9%이었다. 2. 구강내에서 측정된 LDD의 민감도는 76.5%이었고, 특이도는 69.2%이었다. 3. 실험실 연구에서 유치 법랑질의 탈회 기간에 따른 DIFOTI 광투과율의 회귀 분석한 결과, 탈회 시간에 따라 광투과율은 감소하였다(r=-0.96, p<0.05). 4. 실험실에서 탈회 기간에 따른 LFD의 측정값의 회귀 분석 결과 통계적 유의성을 보이지 않았다(p>0.05). 5. DIFOTI 이미지의 광투과율과 CLMS의 병소 깊이에 대한 상관 계수는 -0.688이었으나(p<0.05), LFD의 측정값은 유의성을 보이지 않았다.

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사회연결망분석과 인공신경망을 이용한 추천시스템 성능 예측 (Predicting the Performance of Recommender Systems through Social Network Analysis and Artificial Neural Network)

  • 조윤호;김인환
    • 지능정보연구
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    • 제16권4호
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    • pp.159-172
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    • 2010
  • 협업필터링 추천은 다양한 분야에서 활용되고 있지만 트랜잭션 데이터의 성격에 따라 추천 성능에 현저한 차이를 보이고 있다. 기존 연구에서는 이러한 추천 성능의 차이가 나타나는 이유에 대한 설명을 구체적으로 제시하지 못하고 있고 이에 따라 추천 성능의 예측 또한 연구된 바가 없다. 본 연구는 사회네트워크분석과 인공신경망 모형을 이용하여 협업필터링 추천시스템의 성능을 예측하고자 한다. 본 연구의 목적을 달성하기 위해 국내 백화점의 트랜잭션 데이터를 기반으로 형성되는 고객간 사회 네트워크의 구조적 지표를 측정한 후 이를 기반으로 인공신경망 모형을 구축하고 검증한다. 본 연구는 협업필터링 추천 성능을 예측할 수 있는 새로운 모형을 제시하였다는 점에서 그 의의가 있으며 이를 통해 기업들의 협업필터링 추천시스템 도입에 대한 의사결정에 도움을 줄 수 있을 것으로 기대된다.

온톨로지 기반의 수강지도 시스템 (Ontology-based Course Mentoring System)

  • 오경진;윤의녕;조근식
    • 지능정보연구
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    • 제20권2호
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    • pp.149-162
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    • 2014
  • 수강지도는 학생의 졸업인증이나 공학교육인증 이수를 위해 수강 신청 이전에 수행되는 과정을 지칭한다. 수강지도는 학생의 수강이력 점검과 향후 수강 과목의 안내 등을 포함하여 학생들의 졸업 및 교과과정 인증과 관련된 중요한 역할을 하고 있다. 현재 대부분 대학에서는 수강지도를 위한 전산시스템의 부재로 인해 지도교수가 직접 수동적으로 수강지도를 진행하고 있다. 하지만 이러한 수동적인 방식의 수강지도는 지도교수가 각 학생에 대한 정보를 분석해야 하고, 때때로 휴먼에러를 일으키게 된다. 수강신청이 학기 단위로 이루어지기 때문에 휴먼에러로부터 발생된 피해는 원상태로 되돌리는 것이 거의 불가능하다. 따라서 수강지도를 진행함에 있어 자동화된 시스템은 필수적인 요소로 판단된다. 관계 데이터 모델을 이용한 수강지도 시스템의 도입은 수동적인 수강지도의 문제점을 해결할 수 있게 해준다. 하지만 교육과정 및 인증제도의 변화에 따라 기존 시스템의 스키마 변경이 요구되고, 수강 과목 사이에 존재하는 관계 및 의미적인 검색을 제공하는 것이 어렵다는 한계가 존재한다. 본 논문에서는 수강지도 시스템을 위한 수강지도 온톨로지를 모델링하고, 온톨로지 기반의 수강지도 시스템을 설계한다. 온톨로지 인스턴스 생성을 위해 JENA 프레임워크를 이용하여 온톨로지 생성 모듈을 개발하였고, 실험에 참가한 학생의 수강 이력 데이터를 기반으로 온톨로지 인스턴스를 생성하고 추론과정을 통해 트리플 저장소에 저장하였다. 실험은 제안하는 시스템이 학생들이 향후 수강할 수 있는 과목을 모두 제공하는지 여부와 제공되는 과목에 대한 정보 및 학점 계산들이 정확한 지를 측정하였다. 실제 학생의 수강내역을 이용한 실험의 결과는 온톨로지 기반의 수강지도 시스템이 현 수강지도 시스템의 수동적 방법을 해결하고, 사람이 지도한 내용과 같은 내용을 도출하는 것을 확인함으로써 제안하는 시스템의 유효성을 보여준다.

난소 낭종 제거술후 난소 용적 및 호르몬의 변화 (Assessment of Ovarian Volume and Hormonal Changes after Ovarian Cystectomy in the Different Ovarian Tumor)

  • 박준철;배진곤;김종인;이정호
    • Clinical and Experimental Reproductive Medicine
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    • 제35권2호
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    • pp.155-162
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    • 2008
  • 목 적: 난소 용적 측정 및 기저 FSH, $E_2$, CCCT 검사를 통하여 가임기 여성에서 난소 낭종 제거술후 난소 배란능의 변화를 평가하고자 하였다. 연구방법: 난소 낭종으로 내원한 환자 중 35세 이하의 여성으로서 비교적 규칙적인 생리주기를 가지며 한쪽 난소에만 낭종을 가진 22명을 대상으로 자궁내막종군과 비자궁내막종군으로 구분하여 전향적 비교 분석을 시행하였다. 질 초음파를 이용하여 수술 1개월 전과 수술 3개월 후 생리주기 3일에 난소의 용적을 측정하였다. 또한 수술 전과, 수술 후 두 번의 정상 생리가 있은 뒤 기저 FSH, $E_2$, CCCT를 시행하였다. 대상 환자 22명 중 3명은 술후 검사를 계획한 3개월 전에 임신이 됨으로써 연구대상에서 제외하였다. 결 과: 수술 후 난소 용적은 자궁내막종군에서 $4.79{\pm}2.57\;cm^3$, 비자궁내막종군 중 직경 ${\geq}10\;cm$인 경우에서 $5.21{\pm}1.33\;cm^3$로서 건측과 비교하여 유의한 감소가 있었으나, 비자궁내막종군 중 직경 <10 cm인 경우는 $6.18{\pm}2.85\;cm^3$로서 유의한 용적 감소가 없었다. 수술후 자궁내막종군의 기저 FSH는 $4.25{\pm}0.20\;mIU/ml$, CCCT 10일째 FSH는 $3.79{\pm}0.80\;mIU/ml$였고, 비자궁내막종군은 각각 $4.24{\pm}0.85\;mIU/ml$, $4.28{\pm}0.92\;mIU/ml$로서 수술 전후의 기저 FSH, CCCT 결과 비교에서 각 군 모두 유의한 차이가 없었으며, 두 군간의 비교에서도 유의한 차이가 없었다. 결 론: 자궁내막종 절제술 및 10 cm 이상의 난소 낭종 절제술 후 난소 용적은 유의한 감소를 보였으나 10 cm 미만의 난소 낭종 제거술에서는 난소 용적의 유의한 감소가 없었다. 난소 배란능을 평가하기 위하여 측정한 기저 FSH, CCCT 검사 결과는 낭종 제거술 후에도 각 군 모두 유의한 차이를 보이지 않았다. 따라서 가임기 여성에서는 난소절제술 보다는 낭종 제거술이 우선 고려되어야 하며, 세심한 주의를 기울인다면 난소 배란능의 손상을 최소화할 수 있을 것으로 사료된다.

각종 체온계의 구강체온측정에 관한 실험적 비교연구 -외제화씨 체온계, 전자체온계 및 국산 섭씨체온계에 의한 측정온도와 측정시간의 비교- (COMPARATIVE EXPERIMENTAL STUDY ON MEASUREMENT OF ORAL TEMPERATURE WITH DIFFERENT KINDS OF CLINICAL THERMOMETERS -comparison of Oral Temperature and Oral Placement Time among Fahrenheit Glass Thermometer, Electric Thermometer, Yu II centigrade Glass Thermometer, and Kuk II centigrade Glass Thermometer-)

  • 윤정숙
    • 대한간호학회지
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    • 제4권2호
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    • pp.93-106
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    • 1974
  • The purposes of this study are to identify the necessity of utilization of electric thermometer, to determine the difference of clinical thermometers to reach maximum or optimum temperature, and to determine the length of time necessary for temperature taking, with Fahrenheit thermometer, electric thermometer, Yu Ⅱ centigrade thermometer, and Kuk ll centigrade thermometer. The first and second comparative Experiments were' conducted from August 25 through September 30, 1973. In the first experiment, Fahrenheit thermometer, which had been accurately teated two times, and electric thermometer have been utilized. These two kinds of thermometers were inserted simultaneously under the central area of the tongue and the mouth kept closed while thermometers were in place. All temperature readings were done at one minute interval until leaching-maximum temperature. These procedures were repeated one hundred times and the data were-analyzed statistically by means of the t-test. In the second experiment, Fahrenheit thermometer, which had been accurately tested two. times, Yu Ⅱ centigrade thermometer, and Kuk Ⅱ centigrade thermometer have been utilized. These three kinds of thermometers were inserted simultaneously under the central area of the. tongue and the mouth kept closed while thermometer were in place. All temperature readings were done at one minute interval until reaching maximum temperature. These procedures were. repeated one hundred times and the data were analyzed statistically by means of the F-ratio Under the eight hypotheses designed for this study, the findings obtained are as follows: 1. There were no significant differences in the maximum temperature between Fahrenheit thermometer and electric thermometer. The mean maximum temperature for Fahrenheit thermometers was 37.06℃ and for electric thermometer was 37.09℃. 2. The placement time to reach maximum temperature taken by Fahrenheit thermometer was significantly shorter than that by electric thermometer. The mean placement time for Fahrenheit thermometers was 4.04 minutes, for electric thermometer was 5.52 minutes. In the case of Fahrenheit thermometers, 45 to 77 percent after 3 to 5 minutes, over 90 Percent after 7 minutes, and 100 percent after 10 minutes, had reached optimum temperature. When the electric thermometer was used, 23 to 54 percent after 3 to 5 minutes, over 90 percent after 9 minutes, and 100 percent after 12 minutes, had reached optimum temperature. 5. There ware no significant differences in the maximum temperature among Fahrenheit thermometer, Yu Ⅱ centigrade thermometer, and Kuk Ⅱ centigrade thermometer. The mean maximum temperature for Fahrenheit thermometers was 36.67℃, for Yu Ⅱ centigrade thermometer, was 33.73℃, and for Kuk Ⅱ centigrade thermometers was 37.76℃. 6. There were no significant differences in placement time to reach maximum temperature among Fahrenheit thermometer, Yu Ⅱ centigrade Thermometer, and Kuk Ⅱ centigrade thermometer. The mean placement time (or Fahrenheit thermometers was 7.77 minutes, for Yu Ⅱ centigrade thermometers was 7.25 minutes, and Kuk Ⅱ centigrade thermometers was 7.25 minutes. In the case of Fahrenheit thermometers, 8 to 24 percent after 3 to 5 minutes, over 90 percent after 11 minutes, and 100 percent after 13 minutes, had reached maximum temperature. When the Yu Ⅱ centigrade thermometer was used, 10 to 27 percent after 3 to 5 minutes, over 90 percent after 11 minutes, an8 103 percent after 13 minutes, had reached maximum temperature. When the Kuk Ⅱ centigrade thermometer was used, 11 to 27 Percent after 3 to 5 minutes, over 90 percent after 11 minutes, and 100 percent after 12 minutes, had reached maximum temperature. 7. There were no significant differences in the optimum temperature(the maximum temperature minus 0.1℃) among fahrenheit thermometer, Yu Ⅱcentigrade thermometer, and Kuk Ⅱ centigrade thermometer. The mean optimum temperature for Fahrenheit thermometers was 36.60℃, for Yu Ⅱ centigrade thermometers was 36.69℃, and Kuk Ⅱ centigrade thermometers was 36.69℃. 8. There were no significant differences in placement time to reach optimum temperature among Fahrenheit thermometer, Yu Ⅱ centigrade thermometer, and Kuk Ⅱ centigrade thermometer The mean placement time for Fahrenheit thermometers was 5.70 minutes, for Yu Ⅱ centigrade thermometers was 5.54 minutes, and for Kuk Ⅱ centigrade thermometers was 5.28 minutes. In the case of Fahrenheit thermometers, 21 to 49 percent after 3 to 5 minutes, over 90 percent after 9 minutes, and 100 percent after 12 minutes, had reached optimum temperature. When the Yu Ⅱ centigrade thermometer was used, 23 to 51 percent after 3 to 5 minutes over 90 percent after 10 minutes, and 100 percent after 12 minutes, had reached optimum temperature. When the Kuk Ⅱ centigrade Thermometer was used, 23 to 57 Percent after 3 to 5 minutes, over 90 percent after 9 minutes, and 100 Precent after 11 minutes, had reached optimum temperature.

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본태성 고혈압 환자의 자기실현 및 욕구구조에 관한 연구 (Manifest Weeds and Self-Actualization of Patients with Essential Hypertension)

  • 강익화
    • 대한간호학회지
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    • 제8권1호
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    • pp.163-180
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    • 1978
  • Much of a person's energy is spent in the effort of becoming a productive member of to-day's complex society. This activity may cause tension, and chronic unrelieved tension is an influential factor in blood pressure elevation. The problem of this study was to identify manifest needs and self-actualization of patients with essential hypertension, and to analyse and compare their manifest needs and selt-actualization with the selected general characteristics of We, sex, religion, occupation and level of education with a control group of patients with normal blood pressure readings. The purpose was to contribute to the planning of nursing interventions toward reducing the impact of complex psycho-somatic factors on the anxiety of patients with essential hypertension. The instruments used included selected items from the Edwards (1959) Personal Preference Schedule (EPPS) as adapted by Hwang (1965) and from the Personal Orientation Inventory (POI) (Shostrom 1964, 1974) adapted by Kim and Lee (1977) to measure manifest needs and self-actualization. The convenience sample was chosen from 149 persons who presented themselves for general physical examinations at Ewha University Medical Centre and 41 patients diagnosed with essential hypertension at three general hospitals in Seoul during June 1 and August 31, 1977. Forty-nine persons from the Ewha group with blood-pressure readings exceeding 150/90 were added to the experimental group. Data were analysed by the S.P.S.S. computer programme using t-test and tests for statistical significance. Statistically significant findings were as follows: A. Blood Pressure and Manifest Needs. 1. with the exception of Autonomy, patients with hypertension had significantly high scores on all variables Abasement, Achievement, Affiliation, Aggression, Dominance, Emotionality, Exhibitionism and Sex. 2. When mean scores of normal persons were compared by age groups, normal persons had higher scores in the following order on Abasement (50's, 40's, 20's, 30's), Achievement (50's, 30's, 40's, 20's), Affiliation (50's, 40's, 30's, 20's), Dominance (50's, 40's, 40's, 20's) and Exhibitionism (30's, 50's, 40's, 20's). In each case, there was a significant difference between the first and last age group scores. 3. When the mean scores of normal persons were compared by sex, normal men had higher scores than women on Achievement, Affiliation, Aggression, Dominance, Exhibitionism and Sex. Male patients had higher scores than female patients on Achievement, Dominance, Exhibitionism and Sex, but female patients scored higher in Emotionality. 4. Normal persons had higher scores related to religion in the following order on Achievement (Buddhism, no religion, Christianity). Hyper tensive patients had higher scores on. Exhibitionism (no religion, Christianity, Buddhism). 5. Normal persons had higher scores related to occupation in the following order on Achievement and Exhibitionism (unemployed, office workers, teachless, businessmen), Emotionality (office workers, unemployed, businessmen, teacher) and Sex (office workers, unemployed, teachers, businessmen). Hypertensive patients had higher scores on Achievement and Aggression (teachers, businessmen, office worker, unemployed), Dominance and Exhibitionism (businessmen, teacher, of ace workers, unemployed) and Sex (teachers, office worker, businessmen, unemployed). 6. Normal persons had higher scores related to level of edification in the following order on Abasement, Emotionality and Autonomy (secondary school graduation, university). Hypertensive patients had higher scores on Abasement (no education, primary, university, secondary), Achievement (no education, secondary, university, primary) , Dominance (university, no education, secondary, primary), Exhibitionism (university, secondary, no education, primary), and Sex (university, secondary, primary, no education). B. Blood Pressure and Self_Actualization 1, Patients with hypertension had significantly lower scores on all variables. 2. Normal persons had higher scores related to age groups in the following order on Existentiality (20's, 30's, 40's, 50's). Hypertensive patients showed no significantly different scores. 3. Normal women had higher scores than men on Time Competence. Normal men had higher scores on Feeling Reactivity. Male patients had higher scores than women on Self-Actualizing Value and Self-Regard. 4. Normal persons ha 1 higher scores related to religion on spontaneity (Buddhism, no religion, Christianity). Hypertensive patients had higher scores on Time Competence and Nature of Man (Buddhism, Christianity, no religion). 5. Normal persons had higher scores related to occupation in the following order on Existentiality (teachers, office workers, businessmen, unemployed) and Self-Regard (unemployed, office workers, teachers, businessmen). Hypertensive patients showed no significantly different scores. 6. Normal persons had higher scores related to level of education in the following order on Existentiality and Self-Acceptance (university, secondary). Hypertensive patients had higher scores on inner-Director (university, secondary, no education, primary) and Existentiality (university, secondary, primary, no education). Recommendations for nursing interventions with hypertensive patients with emotional problems or low self-actualization were made. 1. The nurse should encourage the patient through her interactions with other members of the medical team to accept counselling and health education. 2. Through her therapeutic interpersonal relationships with the patient, the nurse should help him discover the causes of his emotional tension. 3. Through her health teaching with the family, the nurse should encourage them to participate with the medical team in the patient's therapeutic plan and in providing him with the minimum possible emotional support. 4. Through frequent counselling with the obsessive-thinking and inflexible patient, the nurse should reevaluate the patient's behaviour and her interventions. 5. Seriously ill patients should be given needed reeducation by members of the professional medical team.

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먹이제한과 단기 수온하강 조건에서 넙치의 스트레스 반응과 생리학적 대사활성 변화 (Changes of Stress Response and Physiological Metabolic Activity of Flounder, Paralichthys olivaceus Following to Food Deprivation and Slow Temperature Descending)

  • 명정인;강덕영;김효찬;이정호;노재구;김현철
    • 한국어류학회지
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    • 제23권2호
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    • pp.87-94
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    • 2011
  • 본 연구는 통상적으로 활어 수송을 위해 사용되는 수온 $15{\pm}2^{\circ}C$ 범위에서 단기 수온 강하와 단기간 먹이제한을 했을 때 넙치의 스트레스 반응과 생리상태를 혈중 생화학 인자와 4종류의 갑상선호르몬의 변화를 통해 파악하고, 이 결과들을 바탕으로 어류의 스트레스 및 생리활성 지표로서 분석 인자들의 효용성을 검토하고자 하였다. 먹이제한 실험은 1년생 넙치 양성어(전장 $32.9{\pm}1.2$ cm, 체중 $348.0{\pm}34.7$ g)를 이용해 8일간 실시하였고, 수온변화 실험은 1년생 양성어(전장 $32.7{\pm}1.2$ cm, 체중 $327.1{\pm}33.6$ g)를 이용해 8일간 수온을 $15.8^{\circ}C$에서 $13.3^{\circ}C$로 하강시키며 실시하였다. 혈중 생화학적 분석 인자는 ALT, AST, TP, GLU, 전해질($Na^+$, $K^+$, $Cl^-$), thyroid hormones (total $T_3$ & $T_4$ free $T_3$ & $T_4$)의 혈중 변화로 이루어졌다. 먹이섭식 제한 결과, AST, ALT 및 전해질에서는 절식기간 동안 어떠한 유의한 변화를 찾아볼 수 없었지만, TP와 GLU는 유의하게 감소하는 경향을 보였다. 갑상선호르몬 농도의 경우, 조사된 4종류 모두 절식기간이 길어지면서 감소하는 경향이 있었으며, 통계적으로 $FT_4$$TT_3$가 개시에 비해 실험 종료시 유의하게 감소하였다. 수온하강 실험의 경우, AST, ALT, TP 및 전해질 및 갑상선호르몬 농도에서 유의한 변화는 없었으나, GLU에서는 온도하강에 따른 농도 감소가 관찰되었다(P<0.05).

생쥐 난소에서 Preantral Follice의 단순 분리법 (A Simple Isolating Method of Preantral Follicles from Mouse Ovaries)

  • 김주환;박기상;송해범;전상식
    • Clinical and Experimental Reproductive Medicine
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    • 제27권3호
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    • pp.235-243
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    • 2000
  • Objective: Our present studies were conducted to examine more effective isolating method of preantral follicles from mouse ovaries. Methods: ICR mice (3-6 weeks old) were sacrificed through cervical dislocation and their ovaries were removed and put into watch glasses containing Hams F-10 supplemented with 10% fetal bovine serum (FBS). Preantral follicles were isolated by three different methods; 1) enzymatical method and 2) mincing method, and 3) scraping method. Enzymatical method was carried out as following. Ovaries were bisected with a pair of fine 30G needles. Bisected ovaries were incubated at $37^{\circ}C$ and 5% $CO_2$ incubator in 2-well dish containing Hams F-10 supplemented with collagenase 600 lU/ml and DNAse 20 lU/ml. After 20 min., follicles were isolated by repeated pipetting. Isolated preantral follicles were collected, and the remnant of tissues was placed in incubator and previous procedure was repeated. Mincing method was carried out with a pair of fine 30G needles attached to 1 ml syringes and minced ovary. Scraping method was carried out with a pair of fine 30G needles and scratched to surface of ovary. The differences between isolating methods were analyzed using Student's t-test and Chi-square. Results were considered statistically significant when ${\rho}$ value was less than 0.05. Results: In handling time, mincing or scraping method ($28{\pm}3.42$ min or $16{\pm}1.58$ min) were significantly (p<0.00001) shorter than enzymatical method ($72{\pm}1.69$ min), and scraping method was significantly (p<0.01) shorter than mincing method. Total number of isolated follicles was significantly (p<0.0001) higher in enzymatical method ($49.8{\pm}3.91$) than in mincing or scraping method ($25.3{\pm}2.33$ or $20.5{\pm}1.75$). Isolated follicles in ${\leq}$90${\mu}m$ were significantly (p<0.005) higher in enzymatical method ($15{\pm}1.71$) than in mincing or scraping method ($7.8{\pm}0.98$ or $8.1{\pm}1.31$). In 91~130 ${\mu}m$, isolated follicles were significantly (p<0.0005) higher in enzymatical method ($33{\pm}3.27$) than in mincing or scraping method ($16.3{\pm}1.82$ or $10.7{\pm}1.38$). In ${\geq}$ 131 ${\mu}m$, isolated follicles were not significantly differences between all groups. In equal sizes, the rate of isolated follicles in ${\leq}$ 90 ${\mu}m$ was highest in scraping method (39.6% vs. enzymatical method: 30.1%, p<0.05; mincing method: 30.9%, p=0.11719, NS). Rate of follicles in $91{\sim}130$ ${\mu}m$ was significantly (p<0.05) lower in scraping method (52.7%) than in enzymatical or mincing method (66.3% or 64.5%). Rate of follicles in ${\geq}$131 ${\mu}m$ was highest in scraping method (8.3% vs. enzymatical or scraping method: 3.6%, p<0.05 or 4.6%, p=0.19053, NS). Conclusions: This study suggests that scraping method is simple and useful for isolation of preantral follicles, because this method reduced handling time and recovered enough follicles. The recovered rate of isolated follicles in diameter of 91 ~ 130 ${\mu}m$ was highest in all methods.

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