• Animal Bioscience
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• v.34 no.12
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• pp.1974-1980
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• 2021

Objective: The objective of this study was to estimate standardized ileal digestibility (SID) of amino acids (AA) in growing pigs fed diets containing increasing levels of copra meal (CM) with β-mannanase supplementation. Methods: Twenty barrows (initial body weight: 34.43±0.11 kg) surgically fitted with T-cannulas at the distal ileum were individually housed in metabolism crates. Pigs were allotted to 5 dietary treatments in a completely randomized design with 4 replicates per treatment. The dietary treatments were: i) NC, negative control, corn-soybean meal (SBM) based diet, ii) PC, positive control, basal diet + 0.10% β-mannanase supplementation (800 IU/kg), iii) CM6, PC diet with 6% CM supplementation, iv) CM12, PC diet with 12% CM supplementation, and v) CM18, PC diet with 18% CM supplementation. A nitrogen-free diet was used to estimate basal endogenous losses of AA for SID calculation. All experimental diets contained 0.5% chromic oxide as an indigestible marker. Each period consisted of a 4-d diet adaptation period and a 3-d ileal digesta collection period. Results: There were no differences in apparent ileal digestibility (AID) and SID of all AA between the NC and PC treatments except that the PC treatment had lower AID and SID of glycine than the NC treatment (p<0.05). There were linear decreases in AID and SID of lysine (p<0.05) and aspartic acid (p = 0.06; tendency) with increasing levels of CM in the diets with β-mannanase. Conclusion: The β-mannanase supplementation had no effect on AA digestibility in pigs fed the corn-SBM based diet but increasing levels of CM reduced SID of lysine and aspartic acid.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.7
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• pp.1090-1095
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• 1999

In order to minimize the occurrence of fatty liver, this study investigated how adding a high level of dietary choline or methionine affected the lipid metabolism of Tsaiya ducks. Feeding trials were conducted with sixty Tsaiya ducks during their growing period, when they were 8-12 weeks old, and during their laying period, 10-14 weeks after the onset of laying when they were 26-30 weeks old. The ducks were randomly assigned to one of three groups: a control group (basal diet), a group in which methionine was added to the basal diet, and a group in which choline was added to the basal diet. The levels of methionine and choline added were twice as high as the levels recommended by the NRC for layer-type chickens. Experimental results indicated that adding choline to the basal diet of growing ducks significantly (p<0.05) increased their body weight, while adding methionine significantly (p<0.05)reduced their body weight. Adding either choline or methionine reduced the ducks' liver fat content in both the growing and the laying periods (p<0.05). Ducks receiving added methionine or choline in their diets displayed enhanced egg production (p<0.05). Adding choline increased serum triacylglycerol (TG) in the laying period (p<0.05). Adding either choline or methionine did not significantly (p>0.05) affect the t-globulin level. Adding methionine increased the activity of liver malic dehydrogenase in both the growing and the laying periods, and increased fatty acid synthetase in the laying period (p<0.05). While adding choline markedly (p>0.05) increased VLDL and apo B in both the growing and the laying periods, it decreased HDL and apo A in the laying period (p<0.05). This study found that adding a high level of choline to the basal diets of Tsaiya ducks in both the growing and the laying periods had beneficial effects. Furthermore, adding methionine affected the lipid metabolism of Tsaiya ducks to a lesser extent than adding choline.

• Journal of Nutrition and Health
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• v.28 no.9
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• pp.809-816
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• 1995

We have studied the effects of buckwheat diet on serum glucose and lipid metabolism in 9 NIDDM volunteers during 2 weeks. The subjects were given dietary counseling in their own homes at 2-3 day intervals throughout experimental periods and the dietary intake were determined by interview and record methods. The intake of calorie, carbohydrate, protein and fat during the buckwheat diet period were not significantly different compared with control diet and body weight was maintained within 1-2kg. The mean total glycohemoglobin, fructosamine, total cholesterol and LDL-cholesterol levels at the end of buckwheat diet were significantly lower than the end of control diet (P<0.05). Fasting serum glucose, insulin and HDL-chloesterol levels were slightly decreased. The mean triglyceride level was increased but it was not significant. These results indicate that buckwheat diet is an effective therapeutic regimen for the control of metabolic derangements in diabetes mellitus.

• Imaging Science in Dentistry
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• v.30 no.1
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• pp.33-48
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• 2000

Purpose : To investigate osseointergration of titanium implants into the tibia of rabbits, which were fed a low calcium diet and irradiated. Materials and Methods : To prepare the experimental model, control group was fed a normal diet and experimental group was fed a low calcium diet for 4 weeks. And then, titanium implants were inserted into the tibia of each rabbit. Experimental group was subdivided into two groups; low calcium diet/non-irradiation group and low calcium diet/irradiation group. The low calcium diet/irradiation group was irradiated with a single absorbed dose of 15 Gy at the 5th postoperative day. At 12, 19, 33, 47, and 61 days after implantation (1, 2, 4, 6, and 8 weeks after irradiation), the bone formation in the bone-implant interface area was examined by light microscopy and fluorescent microscopy. Results and Conclusions: 1. In the control group, there began to form woven bone in the bone-implant interface area at 12 days after implantation. As the experimental time was going on, the amount of bone which was in contact with the implant was increased. 2. In the low calcium diet/non-irradiation group, there began to form woven bone in the bone-implant interface area at 19 days after implantation. Although the amount of bone which was in contact with the implant was increased as the experimental time was going on, the extent of increased bone was weak as compared with control group. 3. In the low calcium diet/irradiation group, there began to form woven bone incompletely in the bone-implant interface area at 19 days after implantation, but there were vascular connective tissues in the bone-implant interface area over the entire experimental period. 4. In the control group and low calcium diet/non-irradiation group, bone labeling bands were observed at 33 days after implantation, which suggests that the bone formation and remodeling was in process, but interstitial bone remodeling was not observed in the low calcium diet/irradiation group.

• Journal of Nutrition and Health
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• v.12 no.2
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• pp.45-51
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• 1979

The present study was undertaken to determine the metabolic effect of various levels and kinds of fat in the diet. Body lipid metabolism at different period of rat's life was also studied by sacrificing rats and collecting samples initially, at second and fourth week of experimental period. Body weight, FER, PER were low in the group fed 30% fat diet. The weight of internal organ and epididymal fat pad was low in high fat diet group. The level of total serum cholesterol of rats in butter group showed higher than that of soybean oil group when the level of dietary fat content was same. The ratio of total cholesterol and total lipid in serum of rats in butter group appear to be higher than that of soybean oil group.

• Journal of Nutrition and Health
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• v.40 no.6
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• pp.500-508
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• 2007

The purpose of this study was to investigate the influence of mixed NaCl-KCl salt on sodium intake and urinary excretion of sodium and potassium. In this study, 3-day food records for pre-experimental diet and 24-hr urine collected for 2-days, 6-day experimental diet food and 24-hr urine were used to evaluate the relationship between Na metabolism. In the experimental diet food, mixed NaCl-KCl salt was added. During the pre-experimental diet period, intakes of Na and K were 178.2 mEq and 56.4 mEq, respectively. The urinary excretion of Na and K in 24-hr were 139.6 mEq, 27.7 mEq, respectively and urinary Na/K ratio was 6.6. During the experimental diet period, intakes of Na and K were 130.2 mEq and 120.4 mEq, respectively. The urinary excretion of Na and K in 24-hr were 100.2 mEq, 37.1 mEq, respectively and urinary Na/K ratio was 2.8. According to this study, it is concluded that mixed NaCl-KCl salt diet decreased the intake of Na, and increased the intake of K.

• Journal of the Korean Society of Food Science and Nutrition
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• v.27 no.4
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• pp.698-704
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• 1998

This study was carried out to investigate the effects of diet of Korean safflower(Carthamus tinctorious L.) seed powder on bone tissue during the recovery of rib-fracture in rats. Male Sprague-Dawley rats of 10 weeks old, weighing 370$\pm$5g, were divided into two groups including the control group(C group, AIN-76 semipurified diet) and safflower seed group(S group, AIN-76 semipurified diet+10% safflower seed powder) and were fed experimental diets for 12 days after adaptation period. After this period, the 9th right rib was fractured surgically and sham-operation was also performed. Rats were fed with experimental diets for up to 30 more days after rib-fracture. The degree of bone repair was evaluated during the recovery period at the 8th, 11th, 16th, 21st, 30th days after the surgical operation by microscopic observation of the fractured rib tissue. In callus formation, the portion of hyaline cartilage was noticably higher in S group than C group. The intracatilagenous ossification was observed at the 8th day in S group, but at 11th day in C group. The intramembranous ossification in callus was widely found over the 8th day to the 11th day in S group, but it was shown over the 11th day to the 16th day in C group. Bone resorption was also occured more rapidly in S group as indicated by large numbers of osteoclasts observed. At the 30th day, most of trabecular bones were disappeared in S group, whereas still shwon in C group over wide ranges of fractured ribs. These results imply that the supplementation of Korean safflower seed powder influences in the recovery of bone fracture by accelerating the process of bone repair.

• Journal of Nutrition and Health
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• v.40 no.8
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• pp.684-692
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• 2007

This study was performed to investigate the effect of chongkukjang intake on lipid metabolism and liver function in ethanol consumed rats. Twenty one Sprague-Dawley male rats aging 4 weeks old were used as experimental animals, which were divided into three dietary groups; casein diet(CA), soybean diet(SB) and chongkukjang diet(CJ). Alcohol was consumed with water as 25%(v/v) ethanol solution. After 4 weeks of experimental period, rats were sacrificed to get blood and liver samples for analysis of lipids, lipid peroxides, antioxidative enzymes and biochemical indices of liver function. The mean body weight, food intake and liver index were not significantly different among three groups. Serum level of total lipid, triglyceride and total cholesterol of chongkukjang diet group was the lowest among three groups although the difference was not significant. HDL-cholesterol level was significantly(p<0.05) higher in chongkukjang diet group than that of casein diet group. LDL-cholesterol level of chongkukjang and soybean diet group was significantly(p<0.05) lower than that of casein diet group respectively. Liver TBARS of chongkukjang and soybean diet group was significantly(p<0.05) lower than that of casein diet group respectively. The superoxide dismutase activity of chongkukjang diet group was significantly(p<0.05) higher than that of casein diet group. Catalase activity was not significantly different among three groups. As indices of liver function, glutamic oxaloacetic transminase(GOT), glutamic pyruvic transminase(GPT), $\gamma$-glutamyl transpeptidase($\gamma$-GTP) and lactate dehydrogenase(LDH) were not significantly different among three groups. Serum alcohol concentration and activities of alcohol dehydrogenase(ADH) and aldehyde dehydrogenase(ALDH) were not significantly different among three groups. The chongkukjang diet seems to give a beneficial effect for improving lipid metabolism by increasing HDL-cholesterol level and SOD activity while reducing liver TBARS level. However, effect on liver function has to be investigated further.

• Korean Journal of Agricultural Science
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• v.46 no.3
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• pp.489-496
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• 2019

A study was conducted to determine the effects of feeding ${\gamma}$-aminobutyric acid (GABA) and hydrochloric acid (HCl) on the growth performance, nutrient digestibility and fecal score in growing pigs. Ninety Duroc ${\times}$ (Landrace ${\times}$ Large Yorkshire) growing pigs with an average initial body weight (BW) of $25.51{\pm}1.63kg$ were randomly allotted to three treatment groups with 6 replications of 5 pigs per replicate pen for each treatment in a 6-week trial period. The treatments were as follows: 1) basal diet (CON); 2) basal diet with 0.05% GABA and 3) basal diet with 1% of a 10% HCl solution. The results showed that GABA supplementation significantly increased the average daily gain (ADG) (p < 0.05) compared with the control during week 4 and the overall experiment period (0 to 6 weeks). However, HCl supplementation had a numerical increase in the ADG compared with the control. The total tract digestibility of dry matter (DM) was greater in GABA group than the CON (p < 0.05). The supplementation of GABA and HCl in the diet of growing pigs had no significant effect on the fecal scores compared with the CON. Experimental results show that supplementation of 0.05% GABA in the diet of growing pigs had a positive effect on the ADG and DM digestibility in growing pigs.

• Korean Journal of Agricultural Science
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• v.49 no.4
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• pp.647-656
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• 2022

The influence of fermented red ginseng (FRG) and red ginseng extract (RGE) on the growth, nutrient digestibility, and meat quality of growing-fattening pigs was evaluated in the present study. In total, 120 growing-fattening crossbred pigs with a bodyweight of 21.77 kg (_SE1.88) were allocated to 3 treatments with 8 repeats of 5 pigs (2 boars and 3 gilts) for a 22-week experimental period. The pigs were supplemented with a basal diet (CON group) or a basal diet containing 0.1% FRG or 0.1% RGE. Pigs fed the FRG diet had a higher (p < 0.05) average daily gain (ADG) from days 1 to 42 and during the whole period. The apparent nutrient digestibility of dry matter (DM) and nitrogen (N) was improved (p < 0.05) in the FRG treatment in week 6 and that of DM in week 10. Moreover, the drip loss of the meat from the FRG pigs was reduced (p < 0.05) on days 1, 5, 7, and 9. Taken together, this study revealed that the supplementation of 0.1% FRG in the diet improved the ADG and the apparent nutrient digestibility of DM and N and decreased the drip loss of the meat. However, the addition of RGE did not affect the growth of the growing-fattening pigs.