• 한국자기공명학회논문지
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• 제15권1호
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• pp.69-79
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• 2011

HP0827 has two RNP motif which is a very common protein domain involved in recognition of a wide range of ssRNA/DNA.We acquired 3D NMR spectra of HP0827 which shows well dispersed and homogeneous signals which allows us to assign 98% of all $^1H_N$, $^{15}N$, $^{13}C_{\alpha}$, $^{13}C_{\beta}$ and $^{13}C$=O resonances and 90% of all sidechain resonances. The sequence-specific backbone resonance assignment of HP0827 can be used to gain deeper insights into the nucleic acids binding specificity of HP0827 in the future study. Here, we report secondary structure prediction of HP0827 derived from NMR data. Additionally, ssRNA/DNA binding assay studies was also conducted. This study might provide a clue for exact function of HP0827 based on structure and sequence.

• BMB Reports
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• 제39권4호
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• pp.464-467
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• 2006

Recently, the nucleotide sequences of entire genomes became available. This information combined with older sequencing data discloses the exact chromosomal location of millions of nucleotide markers stored in the databases at NCBI, EMBO or DDBJ. Despite having resolved the intron/exon structures of all described genes within these genomes with a stroke of a pen, the sequencing data opens up other interesting possibilities. For example, the genomic mapping of the end sequences of the human, murine and rat BAC libraries generated at The Institute for Genomic Research (TIGR), reveals now the entire encompassed sequence of the inserts for more than a million of these clones. Since these clones are individually stored, they are now an invaluable source for experiments which depend on genomic DNA. Isolation of smaller fragments from such clones with standard methods is a time consuming process. We describe here a reliable one-step cloning technique to obtain a DNA fragment with a defined size and sequence from larger genomic clones in less than 48 hours using a standard vector with a multiple cloning site, and common restriction enzymes and equipment. The only prerequisites are the sequences of ends of the insert and of the underlying genome.

• 한국정보과학회논문지:컴퓨팅의 실제 및 레터
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• 제10권3호
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• pp.273-281
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• 2004

동영상의 현재 블록의 움직임 벡터와 이전 블록의 움직임 벡터는 시간적 상관성을 갖고 있다. 본 논문에서는 영상의 시간적인 특성과 움직임 벡터의 가운데 중심 분포 특성을 이용하는 예측 탐색 알고리즘을 제안한다. 제안된 알고리즘은 이전 프레임 블록으로부터 예측된 움직임 벡터와 분할된 탐색 구간에 속하는 후보 벡터 중에서 가장 작은 SAD 값을 갖는 점을 정확한 움직임 벡터를 찾기 위한 초기 탐색점 위치로 결정한다. 실험 결과 제안된 방식은 FS를 제외한 기존의 대표적인 고속 탐색 방식들에 비해 PSNR 값에 있어서 평균적으로 0.19∼0.46㏈ 개선되고 영상에 따라 최고 1.06㏈ 정도 우수한 결과를 나타내었다.

• 한국통신학회논문지
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• 제41권4호
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• pp.404-414
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• 2016

So called quaternary quasi-orthogonal sequence spatial modulation (Q-QOS-SM) has been presented with an advantage of improved throughputs compared to the conventional SM and generalized spatial modulation (GSM) by virtue of a larger set size of QOSs and its minimized correlation value between these QOSs. However the Q-QOS-SM has been originally invented for limited transmit antennas of only powers of two. In this paper, by extending the Q-QOS-SM to any number of transmit antennas, we propose a generalized Q-QOS-SM, referred as G-QO-SM. Unlike the conventional Q-QOS-SM using the Q-QOSs of length of any power of two, the proposed G-QO-SM is constructed based on the Q-QOSs of only the lengths of 2 and 4. The proposed scheme guarantees the transmission of the total $N_t$ spatial bits with $N_t$ transmit antennas, and thus achieves greatly higher throughputs than the other existing schemes including the SM, GSM, Q-QOS-SM, Quadrature-SM, and Enhanced-SM. The performance improvements of the proposed G-QO-SM is justified by comparing the analytically derived BER upper bounds and also the exact Monte Carlo simulation results.

• Molecules and Cells
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• 제24권1호
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• pp.60-68
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• 2007

Microsatellites, also called simple sequence repeats (SSR), are very useful molecular genetic markers commonly used in crop breeding, species identification and linkage analysis. In the present study, we constructed a microsatellite-enriched genomic library of Panax ginseng, and identified 251 novel microsatellite sequences. Tri-nt repeat units were the most abundant (46.6%), followed by di-nt repeats (35.5%). The $(AG)_n$ motif was most common (23.1%), followed by the $(AAC)_n$ motif (22.3%). From the genotyping of 94 microsatellites using marker-specific primer sets, we identified 11 intraspecific polymorphic markers as well as 14 possible interspecific polymorphic markers differing between P. ginseng and P. quinquefolius. The exact allele structures of the polymorphic markers were determined and the alleles were named. This study represents the first report of the bulk isolation of microsatellites by screening a microsatellite-enriched genomic library in P. ginseng. The microsatellite markers could be useful for linkage analysis, genetic breeding and authentication of Panax species.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1995년도 추계학술대회 논문집 학회본부
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• pp.502-504
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• 1995

In video image compression, it is important to extract the exact notion information from image sequence in order to perform the data compression, the field rate conversion, and the motion compensated interpolation effectively. It is well known that the location of the smallest sum of absolute difference(SAD) does not always give the true motion vector(MV) since the MV obtained via full block search is often corrupted by noise. In this paper, we first classifies the input blocks into 3 categories : the background, the shade-motion, and the edge-motion. According to the characteristics of the classified blocks, multiple locations of relatively small SAD are searched with an adaptive search window by using the proposed method. The proposed method picks MVs among those candidates by using temporal correlation. Since temporal correlation reveals the noise level in a particular region of the video image sequence, we are able to reduce the search are very effectively.

• Fisheries and Aquatic Sciences
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• 제16권4호
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• pp.243-251
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• 2013

Genetic diversity and gene flow patterns in Pollicipes mitella were investigated with a nucleotide sequence analysis of 514 base pairs from the mitochondrial cytochrome c oxidase subunit I gene (COI) in 124 samples collected from six Korean populations. In total, 59 haplotypes were defined by 40 variable nucleotide sites in the COI region. The haplotypes had shallow haplotype genealogy and no geographic associations. All populations had high haplotype diversity (0.909 to 0.979) and low nucleotide diversity (0.0055 to 0.0098). The haplotypes with recently diverged nucleotides were distributed by long-range larvae dispersal among regional populations. The pairwise fixation indices ($F_{ST}$) estimated with the exact test and migration rates indicate that substantial gene flow has occurred among populations as a result of sea currents, except between the Uljin (East Sea coast) and other Korean populations. This suggests that significant genetic differentiation and low migration rates have affected the Uljin population.

• 제어로봇시스템학회논문지
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• 제16권1호
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• pp.69-76
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• 2010

The segmentation of moving object in video sequence is a core technique of intelligent image processing system such as video surveillance, traffic monitoring and human tracking. A typical method to segment a moving region from the background is the background subtraction. The steps of background subtraction involve calculating a reference image, subtracting new frame from reference image and then thresholding the subtracted result. One of famous background modeling is Gaussian mixture model (GMM). Even though the method is known efficient and exact, GMM suffers from a problem that includes false pixels in ROI (region of interest), specifically shadow pixels. These false pixels cause fail of the post-processing tasks such as tracking and object recognition. This paper presents a method for removing false pixels included in ROT. First, we subdivide a ROI by using shape characteristics of detected objects. Then, a method is proposed to classify pixels from using histogram characteristic and comparing difference of energy that converts the color value of pixel into grayscale value, in order to estimate whether the pixels belong to moving object area or shadow area. The method is applied to real video sequence and the performance is verified.

• BMB Reports
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• 제38권3호
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• pp.350-353
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• 2005

Alanine at residue 73 (Ala-73) and aspartate at residue 9 (Asp-9) are characteristic to both Cw6 and Cw7 alleles of HLA-C gene and have been suggested as possible markers for psoriasis vulgaris (PsV). However, the results from various ethnic groups/populations are contradictory and inconclusive. In this study, an attempt has been made to examine the association between HLA-C (Ala-73 and Asp-9) and susceptibility to PsV among Saudi patients. Genomic DNA was extracted from 25 Saudi PsV patients and 75 control subjects. Polymerase chain reaction (PCR) was performed to amplify HLA-C sequences using earlier reported primers, C133P and C243PR. Sequence-specific primers were used to specifically detect nucleotide coding for Ala-73 and Asp-9 in all the subjects. The results showed significantly higher frequency of Asp-9 (84.0% versus 61.3%) in PsV patients as compared to controls (p < 0.05, 2-tailed Fisher's exact test). The frequencies of Ala-73 among PsV patients (92%) and controls (88%) did not differ significantly.

• Journal of Welding and Joining
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• 제16권3호
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• pp.129-140
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• 1998

An interface system was developed to offer the welding capability to a robot controller which had not any embedded function for arc welding before, and also an arc sensor algorithm was proposed for weld seam tracking of the welding robot. For the interface system between the robot controller and welding equipments, data communication software and interface connections were composed. The interface system was mae to correspond welding condition, correction data, operation sequence and current status with the robot controller by mutual had shaking and digital signal transfer. Graphic user interface program developed under the environment of windows made it easy to monitor data communication and operation status, and to control welding and sensing sequence. Arc sensing algorithm proposed in this study to compensate torch position error was based on a fuzzy logic with the variables of current difference and current differenced change at torch weaving extremities. The developed interface system could be successfully implemented in between welding equipments and the robot controller, and showed normal status and exact function in data and signal communication between the systems. The whole robot welding system was then examined to verify its welding and seam tracking capabilities in horizontal fillet, vertical fillet, and 3-dimensional fillet weldment. The experiments revealed sound weld bead shapes and also good seam tracing results.