• 한국자원식물학회지
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• 제14권1호
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• pp.84-88
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• 2001

돈분뇨 발효액비를 양채류인 결구상추의 재배에 비료원으로 활용하면서 환경오염 부담을 경감하고자 강원도 고랭지 지역인 대관령에서 수행한 연구결과를 요약하면 다음과 같다. 돈분뇨 발효액을 토양에 처리하였을 때 대조구인 화학비료시용구에 비해서 결구상추의 생육이 증가하는 경향을 보였으며, 수량은 돈분뇨 발효액 처리구에서도 저농도인 50배 희석구에서 가장 높았다. 돈분뇨 발효액 처리시 결구상추에 흡수되는 영양소 함량은 대조구인 화학비용시용구에 비해 증가되는 경향이었으며, 돈분뇨 발효액 희석구와 비교할 때 유의성있는 차이를 보여주지 않았다. 시험전 토양에 비해 돈분뇨 발효액를 처리한 후 pH, EC, 토양의 질소(NH$_4$-N, NO$_3$-N), Av. P$_2$O$_{5}$, Ex. Ca 및 Mg함량은 뚜렷한 변화가 없었다. 본 시험결과 돈분뇨 발효액에는 많은 양의 비료성분을 함유하고 있어 과다 시용시에는 작물피해는 물론 토양이나 지하수를 오염시킬 수 있기 때문에 결구상추의 경우 관비재배시 50배 희석하여 시용하면 작물생육과 환경 측면에서 매우 바람직하다고 판단되었다.

• Animal cells and systems
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• 제3권2호
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• pp.103-113
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• 1999

Many in-depth reviews related to regulations of prolactin secretion are available. We will, therefore, focus on controversial aspects using personal opinion in this review. The neuroendocrine control of prolactin secretion from the anterior pituitary gland involves multiple factors including prolactin-release inhibiting factor (PIF) and prolactin releasing factor (PRF). The PIF exerts a tonic inhibitory control in the physiological conditions. The PIF should be able to effectively inhibit prolactin release or a lifetime, but the inhibitory action of dopamine cannot be sustained for a long period of time. Perifusion of a high concentration of dopamine (l ,000 nM) could not sustain inhibitory action on prolactin release but when a small amount of ascorbic acid (0.1 mM) is added in a low concentration of dopamine (3 nM) solution, prolactin release was inhibited for a long period. Ascorbate is essential for dopamine action to inhibit prolactin release. We have, therefore, concluded that the PIF is dopamine plus ascorbate. The major transduction system for dopamine to inhibit prolactin release is the adenylyl cyclase system. Dopamine decreases cyclic AMP concentration by inhibiting adenylyl cyclase, and cyclic AMP stimulates prolactin release. However, the inhibitory mechanism of dopamine on prolactin release is much more complex than simple inhibition of CAMP production. The dopamine not only inhibits cyclic AMP synthesis but also inhibits prolactin release by acting on a link(s) after the CAMP event in a chain reaction for inhibiting prolactin release. Low concentrations of dopamine stimulate prolactin release. Lactotropes are made of several different subtypes of cells and several different dopamine receptors are found in pituitary. The inhibitory and stimulatory actions induced by dopamine can be generated by different subtype of receptors. The GH$_4$ZR$_7$ cells express only the short isoform (D$_{2s}$) of the dopamine receptor, as a result of transfecting the D$_{2s}$ receptors into GH$_4$C$_1$ cells which do not express any dopamine receptors. When dopamine stimulates or inhibits prolactin release in GH$_4$ZR$_7$ cells, it is clear that the dopamine should act on dopamine D$_{2s}$ receptors since there is no other dopamine receptor in the GH$_4$ZR$_7$. Dopamine is able to stimulate prolactin release in a relatively low concentration while it inhibits in a high concentration in GH$_4$ZR$_7$. These observations indicate that the dopamine D$_2$ receptor can activate stimulatory and/or inhibitory transduction system depending upon dopamine concentrations.

• Reproductive and Developmental Biology
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• 제35권3호
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• pp.329-334
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• 2011

This study was conducted to establish a freezing method of miniature pig spermatozoa. The semen 더aculated from PWG M-type miniature pig was collected by gloved-hand method. The semen was diluted with same volume extender (m-Modena B). The frozen solution used frozen solution of four different (LEY, TCG, BF-5 and m-Modena+egg yolk) for find optimal frozen solution in miniature pig sperm. The diluted semen for frozen rate assay was added to LEY solution (solution I: 11% lactose+egg yolk; solution II: solution I+glycerol+OEP), and frozen depending on freezing rate by the three different freezing methods (A: until $5^{\circ}C$ for 1 hrs, holding at $-102^{\circ}C$ for 10 min; B: until $5^{\circ}C$ for 2 hrs, holding at $-102^{\circ}C$ for 10 min; C: until $5^{\circ}C$ for 3 hrs, holding at -80 and $-102^{\circ}C$ for 10 min). Semen cooled until $5^{\circ}C$ was added with glycerol 1, 3 and 5%, and take a equilibrium time for 0, 10 and 30min. Frozen-thawed sperm were evaluated for viability, acrosomal status and morphological abnormality. The results of frozen-thawed sperm ability by frozen solution, viability was higher in LEY solution compared to other three different frozen solution. AR pattern of LEY solution were lower than other three different frozen solution. The results of freezing rate, viability was higher in B method compared to other methods (p<0.05). Acrosomal statute was intacted in A and B methods than C method. The experiment for glycerol condition was showed that sperm viability was higher in extender with 1% and 3% glycerol and equilibrium time of 0 min. The acrosome damage was lower in extender with 1% glycerol and equilibrium time of 10 min than other conditions. In conclusion, the optimal conditions for cryopreservation of miniature pig spermatozoa obtained in LEY frozen solution, cooling rate of 1~2 hrs, 1~3% glycerol concentrations and glycerol equilibrium time of 0~10 min.

• 멤브레인
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• 제9권3호
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• pp.170-177
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• 1999

Polysulfone을 술폰화하여 물-부탄올 용액의 분리를 위한 투과증발용 비대칭막을 제조하였다. 술론화제로는 chlorosulfoneic acid-trimethyulchlorosilane을 반응시킨 후 이용하였다. FT-IR과 $^1H$-NMR을 이용하여 치환여부를 살펴보았고 술폰화정도는 역적정법과 $^1H$H-NMR을 이용하여 구하였다. DSC와 TGA를 이용하여 열적특성변화를 알아보았다. 비대칭막을 제조하기 위하여 N-methyl-2-pyrrolidone(NMP)rhkdiethyleneglycol dimethyl ether (DGDE)의 혼합용매를 사용하였다. SEM을 이용하여 단면과 skinlayer 의 구조변화를 살펴보았다. 한 연구에서 제조한 비대칭막은 dense film에 비하여 물에 대한 선택도는 거의 변화가 없고 투과유량은 약 80배 정도 증가하였다.

• 한국전자파학회지:전자파기술
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• 제5권2호
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• pp.29-38
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• 1994

정보통신 싯스템이 고속/고밀도화 됨에 딸 전자파 차페 효과와 냉각 성능의 최적 조건을 구하는 문제가 대두되고 있다. 따라서 본고에서는 다중 개구면이 잇는 함체에 대한 전자파 차폐 효과와 냉각 특성을 조사하였다. 차례 효과는 실험치화 이론치를 구하였고 냉각 특성은 컴퓨터 수치 해석을 이용하여 공극율에 따른 특성을 파악하였다. 연구 결과, 개구면의 형상에 따른 차폐 효과는 공극율에 가장 큰 영향을 받으며, 상반된 성질이 있는 냉각 특성과 종합적으로 비교하여 볼대 공극율이80% 이상인 경우에는 특성변화가 급격함을 알 수 있었다.

• KIEAE Journal
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• 제14권5호
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• pp.49-57
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• 2014

This research is about analysis and evaluation of biodiversity of Suwon's street tree, then understanding the problem of it and finally finding a solution. Because the increasing damage of the street trees by disease and insects, insecticide is applied to prevent further damage. However, this insecticide is found to be cancer genic and causing hygienic threat to civilians. Therefore, by gathering Suwon's internal statistics about Suwon's street tree, the trees are divided into three categories, tall evergreen trees, deciduous trees, shrubs following Frank's 30-20-10 theory(1990). Also, according to species diversity index, the problem of disease and insect is researched in terms of biodiversity, and here we suggests solutions to counter such problems. According to the results, the trees planted in Suwon was found to be 31 families, 43 genus and 58 species. The most used kinds, almost 85% of the whole species, are found to be Rhododendron indicum (L.) Sweet, Buxus koreana Nakai ex Chung & al, Euonymus japonicus Thunb, Ligustrum obtusifolium Siebold & Zucc. Besides these, the rest of 15% of street trees had little variety. Therefore, it is necessary to plant tree variously and equally in terms of biodiversity. If this Frank's 10-20-30 solution is not enough to completely solve coulure problem, then further research will be done on soil properties, and local features for improvement of Suwon street tress.

• Journal of Chest Surgery
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• 제33권5호
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• pp.377-384
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• 2000

Background: Numerous studies of safe, long term preservation for lung transplantation have been performed using ex vivo models or in vivo single lung transplantation models. However, a safe preservation time which is applicable for clinical use is difficult to determine. We prepared LPDG solution for lung preservation study. In this study we examined the efficacy of LPDG(low potassium dextran glucose) solution in 24-hour lung preservation by using a sequential bilateral canine lung allotransplant model. Material and Method: Seven bilateral lung transplant procedures were performed using weight-matched pairs(24 to 25kg) of adult mongrel dogs. The donor lungs were flushed with LPDG solution and maintained hyperinflated with 100% oxygen at 1$0^{\circ}C$ for a planned ischemic time of 24 hours for the lung implanted first. After sequential bilateral lung transplantation, dogs were maintained on ventilators for 3 hours: arterial resistance were determined if the recipients hourly after bilateral reperfusion and compared with pretransplant-recipient values, which were used as controls. After 2hours of reperfusion, the chest X-ray, computed tomogram and lung perfusion scan were performed for assessmint of early graft lung function. Pathological examinations for ultrastructural findings of alveolar structure and endothelial structure of pulmonary artery were performed. Result: Five of seven experiments successfully finished the whole assessments after bilateral reperfusion for three hours. Arterial oxygen tension in the recipients was markedly decrased in immediate reperfusion period but gradually recovered after reperfusion for three hours. The pulmonary artery and pulmonary vascular resistance showed singificant elevation(p<0.05 versus control values) but also recovered after reperfusion for three hours(p<0.05 versus immediate period value). The ultrastructural findings of alveolar structure and endothelial structure of pulmonary artery showed reversible mild injury in 24 hours of lung perservation and reperfusion. Conclusion : This study suggests that LPDG solution provides excellent preservation in a canine model in which the dog is completely dependent on the function of the transplanted lung.

• 한국전산유체공학회:학술대회논문집
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• 한국전산유체공학회 2011년 춘계학술대회논문집
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• pp.294-301
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• 2011

A two-dimensional hybrid flaw solver has been developed for the accurate and efficient simulation of steady and unsteady flaw fields. The flow solver was cast to accommodate two different topologies of computational meshes. Triangular meshes are adopted in the near-body region such that complex geometric configurations can be easily modeled, while adaptive Cartesian meshes are, utilized in the off-body region to resolve the flaw more accurately with less numerical dissipation by adopting a spatially high-order accurate scheme and solution-adaptive mesh refinement technique. A chimera mesh technique has been employed to link the two flow regimes adopting each mesh topology. Validations were made for the unsteady inviscid vol1ex convection am the unsteady turbulent flaws over an NACA0012 airfoil, and the results were compared with experimental and other computational results.

• 한국응용과학기술학회지
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• 제31권4호
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• pp.663-668
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• 2014

An in-vivo diagnosis of trace Mg(II) ion was performed using a low-cost and environment-friendly voltammetric method, using a graphite counter and reference electrodes and a fluorine-immobilized graphite working electrode, and clean deep seawater was used as an electrolyte solution. Under optimum conditions, the analytical working ranges attained microgram ranges, and a detection limit of $80.6ugL^{-1}$ was obtained using stripping voltammety with 60 sec accumulation time. Ex-vivo application was performed on fish liver and mice droppings. The developed techniques can be applicable to tumor cell analysis.

• 제어로봇시스템학회:학술대회논문집
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• 제어로봇시스템학회 2000년도 제15차 학술회의논문집
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• pp.61-61
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• 2000

Since FMS(Flexible Manufacturing System) such as semiconductor production systems have the characteristic that each device has to be commonly used in several stages, it is difficult to find an optimal solution. In this paper, we proposed the new algorithm which can get the optimal ratio of sharing resources. We will implement the proposed algorithm to semiconductor production systems. We introduce the optimal algorithm, which is modeled and analyzed by ExSpect, a petri net based simulation tool. When there exist conflicts of sharing resources, the scheduling method is adopted, which gives a priority to the most preceded process. The suggested algorithm can be used not only in semiconductor production systems but also in various FMS.