• BMB Reports
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• 제28권1호
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• pp.72-78
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• 1995

Time-dependent inactivation of E. coli GTP cyclohydrolase I with a 2',3'-dialdehyde derivative of GTP (oGTP) was directed to the active site of the enzyme, and was dependent on the concentration of oGTP. The kinetics of inactivation were biphasic with a rapid reaction occurring immediately upon exposure of the enzyme to oGTP followed by a slow rate of inactivation. The $K_i$ value of oGTP for the enzyme was 0.25 mM. Inactivation was prevented by preincubation of the enzyme with GTP, the substrate of the enzyme. At 100% inactivation, 2.3 mol of [8.5'-$^3H$]oGTP were bound per each enzyme subunit, which consists of two identical polypeptides. The active site residue which reacted with the affinity label was lysine. oGTP interacted selectively with the ${\varepsilon}$-amino group of lysine in the GTP-binding site to form a morpholine-like structure which was stable without sodium borohydride treatment. However, triphosphate group was eliminated during the hydrolysis step. To identify the active site of the enzyme, [8.5'-$^3H$]oGTP-labeled enzyme was cleaved by endoproteinase Lys-C, and the $^3H$-labeled peptide was purified by HPLC. The amino acid sequence of the active site peptide was Pro-Ser-Leu-Ser-Lys, which corresponds to the aminoterminal sequence of the enzyme.

• 한국철도학회논문집
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• 제9권6호
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• pp.761-765
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• 2006

At present, harmonic currents cause serious problems in power conversion system using the semiconductor switching device. Also some of the conversion system provokes harmonic currents against to the main power supply system and causes hindrances for the system. Main power impedance of the traditional LC passive filter method, influences on the filter characteristics and amplifies the harmonics when resonance phenomenon is occurred. And the traditional existing 2 level inverter systems show the limit in capacity of voltage and current in case of occurring sudden load change. So, to solve this problem active filter which uses cascaded H-bridge multi level inverter has been designed and ex-filter system circuits were totally investigated. With multi level active filtering system not only the size of filter but also the size of filter for transformer can be reduced by half and so as to the weight, while the capacity of inverter can be double sized and wave forms can be compensated exactly and precisely. Also by the benefit of the increase in rating capacity, the various currents owing to the load fluctuation can be dealt more steadily. In order to simulate the wave form of harmonics based on the measured data on the AC 25kV high speed Domestic Commercial railway, it was simulated with PSCAD/EMTDC and PSIM. Based on the results of this demonstration, the power supply system and inverter system would be more stable and also promoting its efficiency.

• 한국환경복원기술학회지
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• 제12권2호
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• pp.73-82
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• 2009

The traffic volume of Seoul is extremely high in comparison to other major cities in Korea, and the result has been harmful physical and mental exposure to pollution by Seoulites on a regular basis. The street air pollution is more important than the others, because the air pollution generated by street traffic directly impacts the health of nearby pedestrians. This problem requires urgent attention and resolution. Among the factors creating the air pollution originating from the street, is the configuration of streets, which have emerged as the most significant because it is related to air and pollutant dispersion. Therefore, this study was conducted under the assumption that street form affects the air quality. Study sites were classified by street characteristics, and air quality was analyzed in each class. Then the OSPM (Operational Street Pollution Model) was employed to simulate the relationship between street configuration and air quality of streets within the old city center and new city center in Seoul. After that this study analyzed the correlation between air pollution and the spatial configuration of urban streets (ex. street width, building height, building density, etc.) to determine their contributions to air pollution. The outcome of this study is as follows : First, the result that was derived from the correlation analysis between street configuration and air quality hewed that the air pollution of the street is influenced by the average height of building, width of the roads as well as traffic volume. On the roadside, the concentration level of $NO_2$ is mainly affected by the average height of building and the deviation of building height along the street and CO is affected by street width. The outcome of this study can be used as a basis for more sound urban design policies, and the promotion of desirable street environments for pedestrians.

• 한국세라믹학회지
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• 제35권2호
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• pp.137-144
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• 1998

고온초전도체 61심 Bi-2223 선재간의 초전도 접합부위를 화학적 부식 및 열적· 기계적 반복 공정에 의하여 제조하였다. 초전도 선재 테이프의 은 피복재 한쪽 표면을 초전도체와 반응하지 않는 부식액(NH4OH:H2O2=1:1)으로 화학적으로 제거한 다음, 두 시편을 일출가압 성형하여 접합시편을 제조하였고 일련의 서로 다른 열적· 기계적 처리를 거쳐 접합부의 물성 및 미세구조를 분석하였다. 접합부를 따라 임계전류(Ic) 변화와 전류전압 곡선의 특성을 측정하기 위하여 여러 단자를 접합부 주위에 설치하여 부위별 I~V 특성을 측정한 결과 단심선재에 비하여 다심선재에서 선재 전체의 통전 능력을 좌우하는 천이구간의 임계전류값이 높았다. 그러나 단심에 비해 다심선재는 천이급속도를 나타내는 n값이 다심선재내 각각의 초전도 core들의 상호작용에 의하여 낮은 값을 나타내었다. 접합부의 임계전류 통전성은 반복적인 가압성형 공정과 서냉반응 열처리 공정에 의하여 향상되었다.

• Journal of Nutrition and Health
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• 제30권8호
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• pp.976-986
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• 1997

Coronary artery disease (CAD) such as angina pectoris and myocardial infarction(MI) have been considered the major cause of death for decaddes . THeir incidence and prevalence are still increasing . Numerous studies have been done on the risk factor analysis of CAD in Western countries. Since the diet in Western countries is different from that in Korea it is difficult to assume that the Korean diet has the same effects as its western counterpeart on the development of CAD . THus the gudidelines for the Western CAD patients can no totally be applied to Koreans. This study was conducted to investigate the relationship between diet and CAD in Koreans. Subjects were comprised of patients admitted to the hospital for chest pain with poxxilbe CAD (men 129, women 65) . They were divided into the following three groups according to angiographic results ; angiogrphically normal coronary artery group (control), single vessel disease group(SVD) and multiple vessel disease group (MVD) . Intakes of dietary fatty acids and other nutrients were assessed by the semiquantitative food frequency method. Blood was also obtained from subjects for serum lipid analysis. Serum lipid profiles of men were clearly different form those of women. For men, serum choesterol levels (or LDL or LDL/HDL) seemed to be higher in the MVD group compared to the control and SVD groups, while TG level was significantly higher in the MVD group for women. Both men and women showed significantly higher caloric , fiber and vitamins C and A intakes in the MVD group than in the other two groups. Higher caloric intakes was due to higher intakes of carbohydrate and protein rather than fat intake. In terms of fatty acids intake, there were no significant differences among the three groups . Smoking seemed to have great effect on eating patterns in CAD patients. Smokers and ex-smokers showed significantly higher intakes of most nutrients including individual fatty acids that non-smokers. The results of stepwise regression showed that moderate alcohol intake decreases LDL levels and increases HDL levels and smoking seems to lowe HDL levels in CAD patients.

• 복식
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• 제32권
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• pp.21-30
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• 1997

Chogori the basic upper garment of korea costume occupies an important role in tra-ditional dressing and continues to be in use to the present days. Of course there has been changes in the length and line of Chogori with the flow of time based on the Ancient Yoo. This is a study of the 14 parts of Chgori based on statistical analysis by computing the practical measuements. Here the statistical analysis is a objective and quantitative of the stylistic changes in Chogori with time. In this study from the data the Mean and Standard deviation has been evaluated and periodic change is shown by graph to test the periodic change T-test Regressional analysis Index analysis has been used. The results are as follows: 1. The length of clothing has changed with time except the sleeve length. Here the length of clothing means all the other measurements ex-cept the sleeve Thus while the measurements of sleeve length has been uniquely unchanged the other measurements have influenced each other. 2. Generally the form of Chogori had the tendency towards smallness in the 19th cen-tury. But it tended to get larger in the 20th century. 3. Compared to other periods the mode of 19th and 20th century Chogori was widely ac-cepted as the Standard deviation of that period was very narrow. 4. The results seen from the regressional analysis of the Cho-sun period woman's Chogori satisfy the t-value and R-squared and thus support the regression formula presump-tion. 5. From the index analysis it is revealed that with decrease in the armhole measurement sleeve measurement and neckband; relatively same decrease in the wrist measurement; and very marked decrease in the sideline measurement.

• Applied Science and Convergence Technology
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• 제23권6호
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• pp.309-316
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• 2014

A large particle accelerator requires an ultrahigh vacuum (UHV) system of average pressure under $1{\times}10^{-7}$ Pa for mitigating the impact of beam scattering from the residual gas molecules. The surface inside the beam ducts should be controlled with an extremely low thermal outgassing rate under $1{\times}10^{-9}Pa{\cdot}m^3/(s{\cdot}m^2)$ for the sake of the insufficient pumping speed. To fulfil the requirements, the aluminum alloys were adopted as the materials of the beam ducts for large accelerator that thanks to the good features of higher thermal conductivity, non-radioactivity, non-magnetism, precise machining capability, et al. To put the aluminum into the large accelerator vacuum systems, several key technologies have been developed will be introduced. The concepts contain the precise computer numerical control (CNC) machining process for the large aluminum ducts and parts in pure alcohol and in an oil-free environment, surface cleaning with ozonized water, stringent welding process control manually or automatically to form a large sector of aluminum ducts, ex-situ baking process to reach UHV and sealed for transportation and installation, UHV pumping with the sputtering ion pumps and the non-evaporable getters (NEG), et al. The developed UHV technologies have been applied to the 3 GeV Taiwan Photon Source (TPS) and revealed good results as the expectation. The problems of leakage encountered during the assembling were most associated with the vacuum baking which result in the consequent trouble shootings and more times of baking. Then the installation of the well-sealed UHV systems is recommended.

• 한국환경복원기술학회지
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• 제16권3호
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• pp.63-81
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• 2013

The vascular plants in the Ohseosan and the Bongsusan, Chungnam were listed 439 taxa (9.0% of all 4,881 taxa of vascular plants); 95 families, 268 genera, 339 species, 5 subspecies, 75 varieties and 20 forms. Furthermore, the Ohseosan were listed 339 taxa and the Bongsusan were listed 306 taxa. So, Hemicryptophytes (H) were 107 taxa (24.4%), Therophytes (Th), Geophytes (G) same as were 82 taxa (18.7%) showed high proportional ratio in life form. Based on the list of rare plants, 4 taxa; Aristolochia contorta, Viola albida, Scutellaria insignis (LC) and Scrophularia koraiensis (DD) and endemic plants, 8 taxa; Aconitum pseudolaeve, Ajuga spectabilis, etc. Based on the list of specific plants, 35 taxa; Wisteria floribunda for. floribunda in class IV, 5 taxa (Scutellaria insignis, Scrophularia koraiensis, etc.) in class III, 5 taxa (Acer triflorum, Cymopterus melanotilingia, etc.) in class II, 24 taxa (Pyrus ussuriensis var. ussuriensis, Campanula punctata, etc.) in class I. Based on the list of naturalized plants, 9 families, 20 genera, 24 taxa (Persicaria orientalis, Carduus crispus, etc.) and ecosystem disturbing plants were Rumex acetocella, Ambrosia artemisiifolia. Naturalization rate was 5.5% of all 439 taxa of vascular plants and urbanization index was 7.5% of all 321 taxa of naturalized plants. In particular, for rare plants, in and ex-situ conservation of genetic resources must surely be done, by preserving present natural habitats, discovering additional natural habitats and securing seeds. Moreover, ecosystem disturbing plants require long-term monitoring and consistent management, since not only do they disturb the ecosystem in competition with Korean native species, but damage humans, too.

• Biotechnology and Bioprocess Engineering:BBE
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• 제7권2호
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• pp.67-75
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• 2002

The human complement receptor type 1 (CR 1, C3 b/C4b receptor) is a polymorphic membrane glycoprotein expressed on human erythrocytes, peripheral leukocytes, plasma and renal glomerular podocytes, which consists of transmembrane and cytoplasmic domains with 30 repeating homologous protein domains known as short consensus repeats (SCR). CR1 has been used as an inhibitor for inflammatory and immune system for the past several years. Recently; it is reported that CRl was found to suppress the hyper-acute rejection in xeno-transplantation and can be used to cure autoimmune diseases. A soluble form of CRl, called sCRl, is a recombinant CRl by cleaving the transmembrane domain at C-terminus and has been expressed in Chinese Hamster Ovary (CHO) cells. Several purification methods for sCR1 from CHO cells have been reported, but most of them require complicated steps at high cost. Moreover, such methods are mostly performed under the pH condition apt to denaturing sCR1 and causes sCRl losing its activity. We here report a rapid and efficient method to purify sCR1 from CHO cell. The new method consists of a two-stage of cell culture by cultivating cells in serum medium followed by serum-free medium, and a two-stage of column purification by means of heparin and gel filtration column chromatography. By using this novel method, sCR1 can be purified in a simple and effective way with high yield and purity, furthermore, the purified sCR1 was confirmed to retain its activity to suppress the complement activation in vivo and ex vivo.

• 자원ㆍ환경경제연구
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• 제19권3호
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• pp.459-483
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• 2010

본 연구는 오염을 배출하고 있는 과점기업들이 시장 진입을 내생적으로 결정할 때 정부가 부과할 수 있는 산출세와 진입세로 구성된 이부 환경세를 설계하고 있다. 특히, 시장 산출량에 따라 비례적으로 오염의 외부성이 발생하는 경우 이부 환경세는 후생을 극대화하는 사회적 최적 수준을 달성할 수 있다는 것을 보였다. 또한 논의의 확장으로 규제 과정에서 추정오차가 존재하는 경우 이부 환경세가 사회후생과 정부조세에 미치는 영향에 대해서도 분석하고 있다. 구체적으로, 사회적 이익집단들이 각자 자신들의 이익에 따라 서로 다른 정보를 제공하는 경우 이부 환경세가 시장 산출량, 정부조세, 그리고 사회 후생에 미치는 영향을 정태적으로 비교 분석해 보았다. 결과적으로, 정부가 사회 후생의 손실분과 정부의 조세 수입 모두를 고려한다면, 오염 함수의 기울기에 대한 과대 추정뿐만 아니라 수요 함수의 기울기에 대한 과소 추정에 대해 유의해야 한다.