• 한국환경생태학회지
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• 제4권1호
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• pp.16-22
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• 1990

속리산 지역내에 생육하고 있는 목본식물을 대상으로 현지조사를 하였으며, 총 44과 89속 140종 10변종 및 2품종이 조사되었다. 한편 국내의 몇 지역과 비교하여 속리산 식물상의 식물지리학적인 분포특성을 파악하고자, 백두산, 설악산, 지리산 및 속리산 등 내륙지역 4개지역, 어유정도, 덕적군도, 고군산군도 및 제주도 등 도서지역 4개 지역등 총 8개 지역의 식물상과 상호 비교하였다. 속리산의 식물상은 설악산 및 지리산의 식물상과 가장 유사하였으며, 단풍나무과, 두릅나무과, 참나무과, 피나무과 및 느릅나무과와 같은 북방계 식물 및 노박덩굴과, 메꽃과, 대극과, 갈매나무과, 가지과, 돌나물과, 쐐기풀과 등과 같은 남방계 식물이 유입되어 있는 것으로 해석되었다.

• 식물분류학회지
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• 제42권4호
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• pp.267-274
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• 2012

흰대극과 섬흰대극의 형태적, 유전적 분화를 알아보기 위해 두 종의 14개 자연 집단으로부터 12개의 형태형질과 11개의 동위효소 유전좌위를 조사하였다. 흰대극복합체의 유전적 다양성 측정치(A=1.63, P=44.83, $H_e$=0.198)는 기존의 동북아산 암대극과 두메대극의 보고와 유사하며, 한국산 붉은대극과 대극보다는 약간 낮은 수치를 보여주고 있다. 비록 대부분 두 종사이의 형태형질의 측정범위가 중복되나 흰대극과 섬흰대극은 형태형질의 조합에 의해 구별되며, 이를 기초로 한 전형질도는 한국에 두 종이 분포함을 지지해주고 있다. 하지만 동위효소 자료를 이용한 분석에서는 두 종이 구별되지 않았으며, 이와 같은 두 자료의 불일치는 두 종이 최근에 분화 하였거나 종간 형질이입에 의한 교잡에 의해 이와 같은 결과가 나온 것으로 추측된다.

• Natural Product Sciences
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• 제21권3호
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• pp.176-184
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• 2015

In our search for natural soluble epoxide hydrolase (sEH) inhibitors from plants, an extract of the dried whole plants of Euphorbia supina Rafin was found to significantly inhibit sEH activity in vitro. Phytochemical investigation of E. supina resulted in isolation of 17 compounds (1 - 17), including triterpenes (1 - 4), phenolic compounds (5 - 8), and flavonoid derivatives (9 - 17). The structures of the isolated compounds were established mainly by extensive analysis of the 1D and 2D NMR, and MS data. All of the isolated compounds were evaluated for their sEH inhibitory activity. Among the isolated phenolic compounds, 8 was identified as a significant inhibitor of sEH, with an IC₅₀ value of 15.4 ± 1.3 μM. Additionally, a kinetic analysis of isolated compounds (2, 5, 8 - 11, 13, and 17) indicated that the inhibitory effects of flavonoid derivatives 10 and 11 were of mixed-type, with inhibitory constants (K_i) ranging from 3.6 ± 0.8 to 21.8 ± 1.0 μM, whereas compounds 2, 5, 8, 9, 13, and 17 were non-competitive inhibitors with inhibition K_i values ranging from 3.3 ± 0.2 to 39.5 ± 0.0 μM.

• Applied Microscopy
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• 제38권4호
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• pp.329-338
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• 2008

대극과 대극속 Meleuphorbia아절의 한계와 근연 분류군과의 유연관계를 규명하기 위해 Meleuphorbia아절에 속한 3종과 근연 분류군에 속하는 9종을 포함하여 총 12종의 화분을 광학 및 주사전자현미경으로 관찰하였으며, 8개의 정량 화분 형질을 이용한 수리분석을 바탕으로 2개의 유형으로 구분하였다: 화분의 표면 무늬가 미세유공상인 Type I (E. epicyparissias, E. inermis, E. pillansii, E. obesa, E. bubalina, E. meloformis, E. nesemanii, E. jansenvillensis), 화분의 표면 무늬가 추문상인 Type II (E. longituberculosa, E. monteiri, E. caput-medusae, E. grantii). 화분 형태학적으로 Meleuphorbia아절은 자연분류군이 아닐 가능성이 크며, Meleuphorbia아절에 속하는 종들은 Anthacantha아절, Treisia절, Medusea절에 속한 종들과 유연관계가 깊은 것으로 나타나, 이들을 함께 하나의 분류군으로 처리하는 것이 바람직하다.

• 한국식품저장유통학회지
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• 제24권4호
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• pp.524-528
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• 2017

Ricinus communis, belongs to the family Euphorbiaceae, has been known as medicinal plants for treatment of inflammation, tumors, antidiabetic, hepatoprotective and laxative. Compared to many pharmacological studies, the effect of R. communis extract on regulating adipogenesis as therapeutic drug for treating obesity has not been reported. R. communis extract (RCE) was investigated to determine its effects on the adipogenesis by monitoring the status of $Wnt/{\beta}-catenin$ signaling and factors involving the differentiation of adipocytes. The differentiation of 3T3-L1 cells monitored by Oil Red O staining was inhibited in concentration dependent manner by RCE. The luciferase activity of HEK 293-TOP cells containing pTOPFlash with Tcf4 response element-luciferase gene was increased approximately 2-folds by the treatment of RCE at concentrations of $100{\mu}g/mL$ compared to the control. Activation of the $Wnt/{\beta}-catenin$ pathway by RCE was further confirmed by immunocytochemical analysis which shows an increment of nuclear localization of ${\beta}-catenin$. In addition, safety of RCE was verified through performing neural stem cell morphology assay. Among the identified flavonoids in RCE, isoquercitrin was the most abundant. Therefore, these results indicate that the adipocyte differentiation was significantly reduced by isoquercitrin in R. communis. In this study, RCE suppresses the adipogenesis of 3T3-L1 cells via the activation of $Wnt/{\beta}-catenin$ signaling.

• Advances in Traditional Medicine
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• 제4권1호
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• pp.37-43
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• 2004

The effects of Aegle marmelos (Rutaceae) leaf, Emblica officinalis (Euphorbiaceae) fruit and Ocimum sanctum. (Labiateae) leaf extracts were studied in L-thyroxine (0.5 mg/kg) induced hyperthyroidic mice. Separately combined effects of these three plant extracts and of a commonly used antithyroidic drug, Propyl thiouracil (PTU) were investigated for comparison. Serum concentration of thyroxine $(T_4)$, triiodothyronine $(T_3)$, glucose and the activity of hepatic Glucose 6-Phosphatase (G-6-Pase) were considered as main parameters. Hepatic lipid peroxidation (LPO), superoxide dismutase (SOD) and Catalase (CAT) activities were also studied to reveal the toxic effect of the plant extracts, if any. While exogenous $T_4$ enhanced serum concentration of $T_4$, $T_3$, glucose and the activity of hepatic G-6-Pase, a simultaneous administration of either A. marmelos leaf (1.0 mg/kg), E. officinalis fruit( 30 mg/kg) and O. sanctum leaf (50 mg/kg) extracts, to hyperthyroidic animals decreased all these parameters. However, the effects were more pronounced, as nearly normal thyroid function and serum glucose concentration were exhibited when all three plant extracts were administered together. A decrease in LPO and a concomitant increase in SOD and the CAT activities indicated the safe and antiperoxidative nature of the plant extracts, administered either alone or in combination. Our findings reveal that the three test plant materials exhibit synergistic effects without any hepatotoxicity, suggesting their potential use in the amelioration of hyperthyroidism and/ or hyperglycaemia.

• 한국약용작물학회지
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• 제18권3호
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• pp.201-207
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• 2010

This study was carried out to obtain the information on the antioxidant activity of Phyllanthus. We compared and analyzed three indigenous collections of Phyllanthus: species from Korea, Brazil and Paraguay. Total phenolic acid contents were $1.93{\pm}0.015$ mg/mL in KCS (Korean Collection Species), $1.65{\pm}0.003$ mg/mL in PCS (Paraguay Collection Species) and $1.49{\pm}0.003$ mg/mL in BCS(Brazil Collection Species). In both DPPH radical scavenging activities and SOD-liked activities, KCS was the highest ($67.25{\pm}0.03%,\;78.38{\pm}1.17%$). KCS were also the highest in hydroxyl radical scavenging activities ($95.80{\pm}0.17%$). It turned out that there was no meaningful difference between BCS ($81.64{\pm}0.23%$) and PCS ($81.54{\pm}0.04%$). As for hydrogen radical scavenging activities, there was no statistically meaningful difference between KCS ($36.44{\pm}0.24%$) and BCS ($36.31{\pm}0.33%$) and it was the lowest in PCS ($33.72{\pm}0.15%$). Our results showed that KCS excelled the others not only in antioxidant activity but also in other beneficial effects. This encouraging results may be potentially useful in developing Phyllanthus urinaria L. variety which contains lots of functional elements such as total phenolic acid.

• 식물분류학회지
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• 제39권2호
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• pp.114-119
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• 2009

경북 영천시와 서울 여의도에서 미기록 귀화식물 2종을 발견하였다. 톱니대극으로 이름 붙인 Euphorbia dentata Michx.(대극과)는 경북 영천시 대창면 대창리 중부고속도로에서 확인되었으며, 동속식물인 포인세티아 (E. pulcherrima)와 비교하여 초본이면서 정상엽들이 한결같이 녹색인 특징으로 구별된다. 또한 왕관갈퀴나물로 이름 붙인 Securigera varia (L.) Lassen(콩과)는 서울 여의도 한강둔치에서 확인되었으며, Securigera속은 국내 미기록속이다. 형태적 유사종으로 묏황기속(Hedysarum L.) 식물에 비해 꽃이 담홍색이고 길이 5-10 cm의 꽃자루 끝에 20개 내외의 꽃이 산형으로 붙는 특징으로 구별된다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2019년도 추계학술대회
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• pp.16-16
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• 2019

The Cassava (Manihot esculenta Crantz) is a perennial woody shrub cultivated mainly in the tropics for its starchy tuberous roots. It belongs to the family Euphorbiaceae which also includes rubber (Hevea brasiliensis) and castor bean (Ricinus communis). Among tropical crops, rice, sugarcane, maize and cassava are the most important sources of calories for human consumption. Problems in the propagation of cassava are virus diseases and low rates of seed germination. Thus, a study was undertaken to develop an efficient in vitro mass propagation protocol of Manihot esculenta Crantz. Young and actively growing stem segments were excised from adult plants of cassava. Samples were cut into a 3~4 cm nodal segments with single node after sterilization, and cultivated in the different medium supplemented with various plant growth regulators for 4 weeks. For shoot multiplication, single-node stem segments, approximately 1 cm in length, were taken from in vitro derived shoots and subcultured. After 4~6 weeks, the shoot generation rate was 55.6%, the shoot number and its length were 1.0/explant and 2.3 cm in the most favorable medium composition. Our experiments confirmed that in vitro growth and multiplication of plantlets could depend on its reaction to the different medium composition, and this micropropagation techniques could be a useful system for healthy and vigorous plant production.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.19-19
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• 2020

The Cassava(Manihot esculenta Crantz) is a tropical root crop, originally from Amazonia, that provides the staple food of an estimated 800 million people worldwide. It belongs to the family Euphorbiaceae which also includes rubber (Hevea brasiliensis) and castor bean (Ricinus communis). Among tropical crops, rice, sugarcane, maize and cassava are the most important sources of calories for human consumption. Problems in the propagation of cassava are virus diseases and low rates of seed germination. So we tried to optimize protocols for mass production of somatic embryo amenable to large-scale vegetative propagation of Cassava. After in vitro eight-week culture of leaves of Cassava, the medium which contained the 2,4-D, BAP and IBA showed the highest callus induction rate, embryogenesis callus formation rate and somatic embryo formation in Cassava culture. In the medium with GA₃ and myo-inositol, shoots were most vigorously regenerated from somatic embryos of Cassava. Our experiments confirmed that in vitro growth and multiplication of plantlets could depend on its reaction to the different medium composition, and this micropropagation techniques could be a useful system for healthy and vigorous plant production.