Advances in Traditional Medicine

Kyung Hee Oriental Medicine Research Center (경희대학교 융합한의과학연구소)
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Synergistic interactions of Aegle marmelos leaf, Emblica officinalis fruit and Ocimum sanctum leaf extracts in the regulation of hyperthyroidism and / or hyperglycaemia

  • Panda, Sunanda (Thyroid research unit, School of Life Sciences, Devi Ahilya University) ;
  • Kar, Anand (Thyroid research unit, School of Life Sciences, Devi Ahilya University)
  • Published : 2004.03.30
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Abstract

The effects of Aegle marmelos (Rutaceae) leaf, Emblica officinalis (Euphorbiaceae) fruit and Ocimum sanctum. (Labiateae) leaf extracts were studied in L-thyroxine (0.5 mg/kg) induced hyperthyroidic mice. Separately combined effects of these three plant extracts and of a commonly used antithyroidic drug, Propyl thiouracil (PTU) were investigated for comparison. Serum concentration of thyroxine $(T_4)$, triiodothyronine $(T_3)$, glucose and the activity of hepatic Glucose 6-Phosphatase (G-6-Pase) were considered as main parameters. Hepatic lipid peroxidation (LPO), superoxide dismutase (SOD) and Catalase (CAT) activities were also studied to reveal the toxic effect of the plant extracts, if any. While exogenous $T_4$ enhanced serum concentration of $T_4$, $T_3$, glucose and the activity of hepatic G-6-Pase, a simultaneous administration of either A. marmelos leaf (1.0 mg/kg), E. officinalis fruit( 30 mg/kg) and O. sanctum leaf (50 mg/kg) extracts, to hyperthyroidic animals decreased all these parameters. However, the effects were more pronounced, as nearly normal thyroid function and serum glucose concentration were exhibited when all three plant extracts were administered together. A decrease in LPO and a concomitant increase in SOD and the CAT activities indicated the safe and antiperoxidative nature of the plant extracts, administered either alone or in combination. Our findings reveal that the three test plant materials exhibit synergistic effects without any hepatotoxicity, suggesting their potential use in the amelioration of hyperthyroidism and/ or hyperglycaemia.

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References

  1. Aebi H. (1983) Catalase. In: Methods in enzymatic analysis, edited by Bergmeyer, HU, 3, p. 276, Academic press, New York.
  2. Ahmed RS, Sharma SB. (1997) Biochemical studies on combined effects of garlic (Allium sativum) and ginger (Zingiber officinale Rosc.) in albino rats. Ind. J. Exp. Biol. 35, 841-843.
  3. Ardawi MS, Khoja SM. (1993) Effects of hyperthyroidism on glucose, glutamine and ketone body metabolism in the gut of the rat. Int. J. Biochem. 25, 619-624. https://doi.org/10.1016/0020-711X(93)90671-Z
  4. Baginski ES, Fod PP, Zak B. (1974) Enzyme activities. In: Methods in Enzymatic Analysis, edited by Bregmeyer HU, 2, p. 876, Academic Press, New York.
  5. Emerit J, Chaudiere J. (1989) Free radicals and lipid peroxidation in cell pathology. In: Hand book of free radicals and antioxidants in Biomedicine, edited by Miquel J, Quintanilha AT, Weber H, p. 177, CRC press, Boca Raton, Florida.
  6. Ganong WF. (1995) Review of Medical physiology, pp. 290-305, Appleton and Lange, Connecticut.
  7. Gorska M, Lopaczyski W, Kinalska I. (1989) Effect of acute hyperthyroidism on insulin removal in rats. Exp. Clin. Endocrinol. 94, 345-350. https://doi.org/10.1055/s-0029-1210920
  8. Halliwell B, Gutteridge JMC. (1989) Free radicals in Biology and Medicine, pp. 188-276, Clarendon Press, Oxford.
  9. Hugget A, Nixon DA. (1957) Use of glucose oxidase, peroxidase and O-dianisdine in determination of blood and urinary glucose. Lancet 2, 368-370.
  10. Jamall IS, Smith C. (1985). Effects of cadmium on glutathione peroxidase, superoxide dismutase and Lipid peroxidation in isolated rat hepatocytes. Toxicology Applied Pharmacology 80, 33-42. https://doi.org/10.1016/0041-008X(85)90098-5
  11. Kar A, Panda S. (2003). Ayurvedic Therapies for Thyroid Dysfunction. In: Scientific Basis of Ayurvedic Therapies, edited by Mishra L, p. 194 CRC press, USA, Boca Raton, Florida.
  12. Kar A, Panda S, Bharti S. (2002) Relative efficacy of three medicinal plant extracts in the alteration of thyroid hormone concentrations in male mice. J. Ethnopharmacol. 81, 281-285. https://doi.org/10.1016/S0378-8741(02)00048-X
  13. Kreps EM, Tyurin VA, Gorbunov NV, Maximovich AA, Polyakov VN, Plyusnin W, Kagan VE. (1986). Activation of lipid peroxidation on Oncorhynchus gorbuscha during spawning migration stress: a possible mechanism of adaptation. Dokl. Akand. Nauk. 286, 1009-1012.
  14. Lowry OH, Rosebrough NJ, Farr AL., Randal RJ. (1951) Protein measurement with the Folin phenol reagent, J. Biochem. 193, 265-275.
  15. Marklund S, Marklund G. (1974) Involvement of superoxide anion radical in the autoxidation of pyrogallol: a convenient assay for superoxide dismutase. Eur. J. Biochem. 47, 469-474. https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
  16. Mirsalis JC, Hamilton CM, Schindler JE, Green CE, Dabbs JE. (1993) Effects of soya bean flakes and liquorice root extract on enzyme induction and toxicity in B6C3F1 mice. Food Chem. Toxicol. 31, 343-350. https://doi.org/10.1016/0278-6915(93)90189-6
  17. Ohkawa H, Ohishi N, Yagi K. (1999) Assay of Lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem. 95, 351-358.
  18. Panda S, Kar A. (1998) Ocimum sanctum in the regulation of thyroid function in male mouse. Pharmacol. Res. 38, 107-110. https://doi.org/10.1006/phrs.1998.0338
  19. Panda S, Kar A. (1999) Guggulu (Commiphora mukul) induces triiodothyronine production: possible involvement of lipid peroxidation. Life Sci. 65, 137-141.
  20. Panda S, Kar, A. (2000) Regulation of hyperthyroidism by R. serpentina root extract in mice. Phram. Pharmacol. Commun. 6, 517-520. https://doi.org/10.1211/146080800128735629
  21. Panda S, Kar A. (2001) Inhibition of $T_3$ production in Levo-thyroxine-treated female mice by the root extract of Convolvulus pluricaulis. Hormone and Metabolic Research 33, 16-18. https://doi.org/10.1055/s-2001-12620
  22. Panda S, Kar A. (2003) Possible amelioration of hyperthyroidism by the leaf extract of Annona squmosa. Curr. Sci. 84, 1402-1403.
  23. Panda S, Bharti S, Kar A. (2002) Emblica officinalis and Bauhinia purpurea in the regulation of thyroid function and lipid peroxidation in male mice. J. Herbs Spices Med. 10, 1-9.
  24. Pari L, Saravanan G. (2002) Antidiabetic effect of Cogent db, a herbal drug in alloxan-induced diabetes mellitus. Comp. Biochem. Physiol. 131, 19-25. https://doi.org/10.1016/S1096-4959(01)00474-2
  25. Rao VV, Dwivedi SK, Swarup D, Sharma SR. (1995). Hypoglycemic & antihyperglycaemic effects of A. marmelos leaves in rabbits. Curr. Sci. 69, 932-933.
  26. Roith DL, Taylor SI, Olefsky JM. (1996) Diabetes Mellitus: A fundamental and clinical text, pp. 500-501, Lipponcot-Raven Publishers, Philadelphia.
  27. Seema PV, Sudha B, Pandayatti PS, Abraham A, Raghu KG, Paulose CS. (1996) Kinetic studies of purified malate dehydrogenase in liver of streptozotocin-diabetic rats and the effect of leaf extract of Aegle marmelos (L) Correa ex Roxb. Ind. J. Exp. Biol. 34, 600-602.
  28. Seiss H. (1993) Strategies of antioxidant defense. Eur. J. Biochem. 215, 213-219. https://doi.org/10.1111/j.1432-1033.1993.tb18025.x
  29. Sharma MK, Kumar M, Kumar A. (2002) Ocimum sanctum aqueous leaf extract provides protection against mercury-induced toxicity in Swiss albino mice. Indian J. Exp. Biol. 140, 1079-1082.
  30. Tahiliani P, Kar A. (2003a) Mitigation of Thyroxine induced hyperglycaemia by two plant extracts. Phytother. Res. 13, 11-15.
  31. Tahiliani P, Kar A. (2003b) Combined effects of Trigonella & Allium extracts in the regulation of hyperthyroidism. Phytomedicine 10, 665-668. https://doi.org/10.1078/0944-7113-00277
  32. Visser TJ, Fekkes D, Docter R, Hennemann G. (1978) Sequential deiodination of thyroxine in rat liver homogenate. Biochem. J. 174, 221-229. https://doi.org/10.1042/bj1740221
  33. Williamson EM. (2001) Synergy and other interactions in phytomedicine. Phytomedicine 8, 401-409. https://doi.org/10.1078/0944-7113-00060

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