• 한국미생물·생명공학회지
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• 제19권1호
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• pp.14-20
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• 1991

Pseudomonas syringae의 intact cell에서 에틸렌 생성을 극대화 하기 위한 전환조건은 $30^{\circ}C$. pH7.5로 조사되었고, 다양한 기질의 전환효과를 검초한 결과, Asn>Gln>Asp>Glu>$\alpha$-KG>citrate>oxalacetate의 순으로 많은양의 에틸렌을 생성하였다. 또한, arginine과 histidine을 상기 유기산과 함께 넣었을 때 에틸렌 생성에 현저한 상승효과를 나타냈다. Cell-free system 에서는 $\alpha$-KG>Glu>citrate>Gln>Ser순으로 0.5mM $\alpha$-KG에서 310.8(nl.mg $protein^[-1}.h^[-1}$)로 가장 많은 에틸렌을 생성하였고, aminotransferse 억제제인 AOA를 사용해 본 결과, Glu는 glutamate dehydrogenase에 의하여 $\alpha$-KG를 거쳐서 에틸렌으로 전환된 것이라 생각된다.

• 농업과학연구
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• 제43권3호
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• pp.340-345
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• 2016

The kiwi fruit (Actinidia deliciosa cv. 'Hayward') should be ripened at any step during postharvest handling before consumer consumption. This is essential for freshly harvested kiwi fruit. But, this requires correct temperatures and ethylene concentrations. More testing of a newly developed ethylene generator using charcoal for commercial purposes is needed. This study was conducted to investigate the optimum storage temperatures and the effect of ethylene on the postharvest quality of kiwi fruit. Three different ethylene concentrations of 10, 50, and $100{\mu}L{\cdot}L^{-1}$ were used on fresh kiwi fruit stored at different temperatures of 10, 15, and $20^{\circ}C$. The quality changes of the fruits were assessed by sensory evaluation and by measuring firmness, soluble solids content, titratable acidity, and ethylene production. Higher storage temperatures and ethylene concentrations softened the kiwi fruit quickly and led to the rapid loss of acidity while soluble solid contents of fruit increased to a significant extent during the same storage period. Similarly, the firmness of ethylene-treated fruits stored at 20 and $15^{\circ}C$ dramatically decreased in the experiment while treated fruits stored at $10^{\circ}C$ decreased only slightly. Quality characteristics of kiwi fruits stored at 15 and $20^{\circ}C$ were better than those of fruits at $10^{\circ}C$. With regards to the effect of temperature, fruits stored at lower temperatures took a longer time to ripen and retained their quality longer. The newly developed ethylene generator maintained the ethylene concentration in the 5 kg box at $40-400{\mu}L{\cdot}L^{-1}$. The ethylene generator could also be used to soften persimmons.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.505-508
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• 2001

An microorganism able to degrade ethylene glycol(EG) was developed. Using this microorganism, biological treatment of ethylene glycol was studied in Erlenmeyer flasks and a laboratory scale stirred loop bioreactor. The removal efficiencies of ethylene glycol from synthetic wastewater were 91.6% ${\sim}$ 97.7% at $30^{\circ}C$ ${\sim}$ $40^{\circ}C$, and 96.3% ${\sim}$ 97.9% at initial pH 9 ${\sim}$ 11 respectively. Also the removal efficiencies of ethylene glycol were found to be more then 92% at initial ethylene glycol concentration of 300mg/L ${\sim}$ I400mg/L. In treatment of weight loss treatment wastewater using Erlenmeyer flasks, the removal efficiencies of ethylene glycol were 79.6%. 82.5%. 77.6%. and 71.3% at initial pH 9. 10. 11. and 12.4 after 11 days of reaction. Moreover in treatment of complex dyeing process wastewater. the residual ethylene glycol was not detected at the initial pH 10.0 and pH 11.3 after 4 days of reaction. When stirred loop bioreactor was used for removing ethylene glycol, the residual ethylene glycol was not detected after 108 hrs and 60 hrs of reaction in batch treatment of weight loss treatment wastewater and complex dyeing process wastewater.

• 한국대기환경학회지
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• 제12권3호
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• pp.333-340
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• 1996

The relationship between ozone-induced damages and ethylend evolution was examined in tomato plants fumigated with ozone of 0.2 $\mu\ell/\ell$. The rate of evolution of ethylent by tomato plants was enhanced by ozone fumigation. Pretreatment of leaves with aminoethoxyvinylglycine (AVG), an inhibitor of ethylene evolution, significantly inhibited the evolution of ethylene that was induced by ozone and concomitantly reduced the extent of ozone-induced visible damage to leaves. Treatment with 2,5-norbonadiene (NBD), and inhibitor of the action of ethylene, strongly reduced the extent of visible damage caused by ozone, even though it did not suppress the evolution of ethylene. These results indicated that ethylene might play an important role in ozone-induced plant injuries at relatively short terms of ozone fumigation. Next, we examined the effect of tiron, a scanvenger of the free-radical, on evolution of ethylene and leaf injury caused by ozone. Tiron treatment strongly reduced the extent of ozone-induced injury, but had not inhibitory effect on the evolution of ethylene from tomato leaves. This result suggests the involvement of free-radical, such as superoxide radicals, in induction of injuries caused by ozone.

• 한국포장학회지
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• 제23권2호
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• pp.97-101
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• 2017

Fruits are subjected to a variety of vibration stress during the transportation from a production area to markets. Vibration inputs are transmitted from the transporting vehicle through the packaged fruit. And the steady state vibration input may cause serious internal damage of fruit. Product quality of fruits declines by various factors while they are stored right after harvesting and among the substance in charge of post ripening action, ethylene ($C_2H_4$) biosynthesis increases fruits' respiration process after harvesting and decreases storage expectancy. Ethylene production of apples rapidly increases while storage duration becomes longer. This tendency is much clearer for the apples with vibration stress at input acceleration level. When there was no vibration stress, change in ethylene production level of apples are not very large during storage. Ethylene production rates inside the gas collecting container increased significantly ($p{\leq}0.05$) after 24 hours storage, particularly for apples with vibration stress ($0.7{\mu}l/kg{\cdot}hr$ (1st stack), $0.78{\mu}l/kg{\cdot}hr$ (2nd stack), $0.96{\mu}l/kg{\cdot}hr$ (3rd stack)); whereas less ethylene was produced in control apples ($0.18{\mu}l/kg{\cdot}hr$ during storage. Also ethylene production rates of apples according to the stack position were significantly different ($p{\leq}0.05$). The vibration stress clearly accelerated the degradation of apple quality during storage, resulting in increased ethylene production.

• Journal of Plant Biology
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• 제35권4호
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• pp.425-429
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• 1992

Ranunculus sceleratus 엽병의 세포 신장은 에틸렌에 의하여 촉진되는 것으로 알려져 있다. 오옥신을 처리한 엽병조직 절편에서 spermine은 세포 신장과 에틸렌의 생성을 비슷한 양상으로 억제하였다. Spermine 농도에 대한 오옥신 유도 에틸렌 생성 억제반응은 ACC에 의한 에틸렌 생성의 경우도 유사한 양상을 나타내었으며 이는 폴리아민이 ACC가 에틸렌으로 전환되는 과정을 억제한다는 것을 시사한다. 오옥신 유도 에틸렌 생성은 폴리아민 생합성 억제제인 DFMA아 DFMO에 의하여 각각 현저하게 촉진되었으며 DFMA에 의한 에틸렌 생성의 증가는 spermine을 고농도로 처리하므로써 완전히 소멸되는 결과를 얻었다. 이러한 결과들은 오옥신과 에틸렌에 대한 Ranunculus의 세포성장 반응에서 내생 폴리아민이 조절 역할을 수행한다는 가능성을 입증하는 것이다.

• 식물조직배양학회지
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• 제25권6호
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• pp.515-518
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• 1998

Ethylene이 옥화란 rhizome의 증식과 유식물체의 분화에 미치는 영향을 조사하였다. Ethylene 발생량은 rhizome 치상 8일째 가장 많았고 배양기간 동안의 총 ethylene 발생량은 rhizome이 증식될 때보다 유식물체가 분화될 때 많았다. 배양 rhizome에 ethephon을 처리한 결과 ethephon은 rhizome의 증식과 유식물체의 길이생장은 억제하였으나 유식물체의 분화에는 효과적이었다. Aminoethoxyvinylglycine(AVG) 1mg/L 처리에서는 rhizome의 증식과 유식물체의 분화에는 효과적이었으나 10mg/L에서는 rhizome의 증식이 억제되었고 유식물체의 길이생장은 촉진되었다. $\textrm{AgNO}_{3}$처리는 rhizome의 증식과 유식물체의 분화를 억제하였다.

• Plant Biotechnology Reports
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• 제3권4호
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• pp.347-353
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• 2009

Procedures that induce microspore embryogenesis have been described for a range of Brassica species, but embryo yield remains low for a number of genotypes. We have carried out experiments with the microspores from a weakly responsive line of B. napus to determine the culture conditions that optimize their in vitro embryogenesis by treating them with effectors of ethylene synthesis and action. The results revealed that isolated microspores subjected to an initial heat stress in a medium supplemented with inhibitors of ethylene synthesis such as AVG and $CoCl_2$ exhibited significantly increased embryo yields. This suggested that regulatory effects are exerted by the ethylene produced by the isolated microspores on the early processes of gametogenesis. As a consequence, treatment of microspores with SAM, an ethylene synthesis precursor, or with the ethylene-releasing agent ethephon, led to decreases in embryo yield. A special response to ethylene during the early stages of microspore development was finally shown to occur through experiments where isolated microspores were treated for increasing periods of time with $CoCl_2$. Collectively, our data demonstrated that the induction of embryogenesis induced by heat stress can be enhanced by inhibitors of ethylene biosynthesis.

• 자연과학논문집
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• 제8권2호
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• pp.169-173
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• 1996

사과 과실의 PE필름 포장 저장 중 에틸렌을 제거하여 저장성을 증대시키기 위한 에틸렌흡착제의 사용이 과실의 저장력에 미치는 영향을 조사하고 자 본 시험을 수행하였다. 에틸렌흡착제 처리는 저장 중 경도저하가 현저하게 억제되었고 흡착제양이 많을수록 억제효과가 크게 나타나는 경향을 보였으나 과실의 당함량과 산함량에는 영향이 없었으며, 과실의 에틸렌 발생량과 호흡량이 감소되었다. 에틸렌흡착제의 처리량은 과실 10kg 당 30g 이상이 적당하였다.

• 전기화학회지
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• 제18권4호
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• pp.150-155
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• 2015

본 연구에서는 glycerol-1,2-carbonate와 4-chloromethyl styrene을 함유하는 공중합체를 합성하고, poly(ethylene glycol) methyl ether 와의 Williamson 반응을 이용하여 poly(ethylene glycol)이 가지로 도입됨과 동시에 높은 유전상수의 에틸렌 카보네이트를 함유하는 고분자전해질을 제조하였다. 흥미롭게도 전해질의 상온 이온전도도는 7 mol%의 에틸렌 카보네이트를 포함하는 가지형 고분자에서 $1.75{\times}10^{-5}S\;cm^{-1}$으로 가장 높게 얻어졌고, 이때 [EO]:[Li] 비율의 최적화는 32:1이었다. 또한 에틸렌 카보네이트기의 존재에 의해 고분자전해질의 전기화학적 안정성을 5.5 V까지 확보할 수 있었다.