• 식물조직배양학회지
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• 제27권4호
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• pp.345-352
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• 2000

Food production is a major role of agriculture. It has been projected that the world population continues to increase by the middle of the 21st century, and the population growth results in raising a serious problem of food shortage. Thus we have to increase food as possible. A considerable amount of crops have been abandoned due to short-life after postharvest. Ethylene is a factor responsible for the postharvest loss in crops, especially horticultural crops. If we can reduce ethylene production or sensitivity by genetic engineering, we can develop, so called,“long-life crop”conferring long postharvest lives. During last two decades, intensive research for molecular dissection of ethylene biosynthesis has been carried out, and the researchers have succeeded in engineering ethylene productivity in some crops. On the other hand, after the successful isolation of Arabidopsis ethylene receptor gene ETR1, the homolog genes have been isolated in various plant species. Currently the characterization of these genes and alteration of ethylene sensitivity using the genes are in progress. This review summarizes current progress in the analysis of these genes, and discusses genetic engineering of ethylene sensitivity using these genes.

• Journal of Plant Biology
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• 제37권3호
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• pp.257-262
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• 1994

The tomato (Lycopersicon esculentum Mill.) mutant diageotropica (dgt) lacking normal gravitropic response is known to be less sensitive to auxin compared with its isogenic parent VFN8. Straight growth as well as ethylene production in response to added auxin in hypocotyl segments of dgt was negligible. However, there was no significant difference between the two genotypes in auxin transport in petiole segments and its inhibition by the phytotropin N-1-naphthylphthalamic acid(NPA). Kinetic parameters of NPA binding to microsomal membranes were also non-distinguishable between the two. Its petiolar explants treated with ethylene developed epinastic curvature with the magnitude of response increased about 3 folds over non-mutant wild type. Ethylene-induced epinasty in both dgt and VFN8 was nullified by treatment of explants with the ethylene autagonist 2,5-norbonadiene. Lateral transport of 3H-IAA toward the upper side of ethylene-treated petioles in dgt, however, was not significantly more pronounced than in VFN8, the implications being that auxin sensitivity in the mutant was restored, or even rised above the wild type, by ethylene.

• 한국산림과학회지
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• 제82권4호
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• pp.328-336
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• 1993

$SO_2$에 의한 조경수목(造景樹木)들의 피해(被害)의 감수성(感受性)을 조사하기 위하여 무처리(無處理), 0.5, 1.5 및 2.5ppm의 $SO_2$ 가스를 각각 하루에 4시간(時間)씩 6일간(日間) 처리(處理)하면서 가시피해율(可視被害率)과 내생(內生) ethylene 발생량(發生量)을 측정(測定), 분석(分析)하였다. $SO_2$ 농도(濃度)에 따른 묘목(苗木)들의 가시피해율(可視被害率)은 0.5ppm 이하에서는 조사대상(調査對象) 수종(樹種) 모두 피해증상(被害症狀)이 없었으며, 1.5와 2.5ppm 수준(水準)에서 경시적(經時的)으로 변화(變化)가 나타났는데, 고농도(高濃度) 일수록 피해율(被害率)이 높았으며, 평균(平均) 피해율(被害率)은 튜립나무가 가장 높았고 다음으로 스트로브잣나무, 은행나무, 소나무, 잣나무의 순(順)이었다. $SO_2$ 처리(處理)에 의한 내생(內生) ethylene의 발생량(發生量)을 보면, 고농도(高濃度) 처리구(處理區)에서는 저농도(低濃度)에 서보다 ethylene의 발생량(發生量)이 많았으며, 발생량(發生量) 최대점(最大點)에 도달되는 시간(時間)도 빨랐다. Ethylene 발생량(發生量)은 튜립나무가 은행나무에서보다 많았으며, 침엽수류에서는 스트로브잣나무, 소나무, 잣나무 순(順)으로 많았다. 특히 가시피해율(可視被害率)이 80% 이상(以上)이었던 스트로브잣나무는 가시피해율(可視被害率)이 증가(增加)함에 따라 ethylene 발생량(發生量)도 직선적(直線的)으로 증가(增加)하다가 가시피해율(可視被害率)이 40-50% 이상에 도달한 이후 ethylene 발생(發生)이 오히려 감소(減少)하는 경향(傾向)을 나타내었다.

• 센서학회지
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• 제5권6호
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• pp.7-14
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• 1996

저온에서 동작 가능한 센서를 개발하기 위하여 $Co_{3}O_{4}$ 후막 소자를 제조하였다. $Co_{3}O_{4}$ 분말은 질산코발트 수용액으로부터 침전하여 얻었으며 binder인 ethylene glycol과 혼합하여 알루미나 기판상에 후막을 인쇄하였다. 제조된 후막에 대해 binder 함량 및 열처리 조건에 따른 부탄가스의 감도 및 회복성을 조사하였다. 동작 온도 $250^{\circ}C$에서 15% ethylene glycol을 함유한 $Co_{3}O_{4}$ 분말을 $300^{\circ}C$ 에서 24시간 동안 열처리한 소자가 부탄가스에 대하여 최고의 감도를 나타내었다. 감도는 500ppm 부탄가스에 대하여 1.1 정도로, 동작 온도 $350{\sim}400^{\circ}C$에서 상용 $SnO_{2}$ 가스 감지 소자의 감도 $0.8{\sim}0.85$와 비교할 때 매우 높았다. 응답 특성은 매우 좋았으나 회복성은 나쁜 것으로 나타났다.

• 센서학회지
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• 제17권4호
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• pp.303-307
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• 2008

$NiO,\;Cu_2O,\;Mn_2O_3$ and $Cr_2O_3$ as p-type semiconductors were added in CoO with 15 wt.% ethylene glycol binder and measured the butane gas sensing characteristics. The highest sensitivity is obtained for the NiO-CoO sensors. CoO-20 at.% NiO sensor with 15 wt.% ethylene glycol binder sintered at $1100^{\circ}C$ for 24 h exhibits high sensitivity of 90 % to 5000 ppm butane gas at the sensor temperature of $250^{\circ}C$, compared to low sensitivities at the low operating temperature for commercial sensors. Response and recovery times are, respectively, within few seconds and 1min in the static flow system, indicating rapid adsorption and desorption of butane gas on sensor surface even at this low temperature.

• 한국환경보건학회지
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• 제32권2호
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• pp.164-170
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• 2006

Ethylene oxide is a genotoxic carcinogen with widespread uses as industrial chemical intermediate and gaseous sterilant. 2-hydroxyethylated N-terminal valine in Hb is a good biomarker for biological monitoring of ethylene oxide exposure, because of its stability. For measuring the hemoglobin adduct formed by exposure of ethylene oxide, we studied the determination of (N-2-hydroxy-ethyl)valine(HEV) in hemoglobin adduct by using GC/MS. Firstly we synthesized HEV with 2-amino-ethanol and bromoisovaleric acid(BIVA) and confirmed it with GC/MS-FID. Its fragmentations were m/z 116(base ion, M+-45) and m/z 130(M+-31). For measuring HEV with higher sensitivity, we use derivatives which were PFPITH(pentafluorophenylisothiocianate) and TBDMS (tributyldimethylsilylation) by using Edman procedure. Its fragmentation were m/z 425(M+-57), m/z 383(M+-99) and m/z 172(M+-310) by using GC/MS. We did biological monitoring for mice inhalation exposure with 400 ppm ethylene oxide. The concentrations of hemoglobin adduct were $168{\pm}3.8\;and\;512{\pm}04$(nmol g-1 globin) at 0.5 hr/day 400 ppm ethylene oxide inhalation exposure group, and $631{\pm}17\;and\;2265{\pm}9.4$(nmol g-1 globin) at 1.0 hr/day 400 ppm ethylene oxide inhalation exposure for 1 and 4 weeks, respectively. We confirmed that (N-2-hydroxy-ethyl)valine(HEV) of hemoglobin was a good biomarker for biomonitoring of ethylene oxide exposure, and can measured with derivatives such as PFPITH(pentafluorophenylisothiocianate) and TBDMS(tributyldimethylsilylation) by using GC/MS.

• Journal of the Korean Wood Science and Technology
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• 제44권4호
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• pp.526-538
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• 2016

Fumigants are used worldwide for control of biological agents that damage wooden cultural property. To establish a policy for fumigant use, biological evaluation of insects and microorganisms considering many factors is required. This study was performed to evaluate biological sensitivity and wood penetration of three fumigants applied for control of biological agents that damage wooden cultural properties in Korea. Among these, methyl bromide and ethylene oxide can control insects and fungi when exposed directly. However, they were unable to completely control biological agents within deeper parts of wood. Ethanedinitrile, which was developed as an alternative fumigant, exhibited outstanding wood penetration and biocidal efficacy. Further research involving various environmental conditions is warranted.

• 센서학회지
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• 제23권4호
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• pp.229-233
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• 2014

In this study, a V-grooved single-mode fiber along with optical time domain reflectometry (OTDR) as a quasi-distributed temperature sensor was investigated. The external medium used to fill the V-groove affects the optical mode. The V-groove was filled with ethylene vinyl acetate (EVA) because its transmittance was sensitive to temperature. The experimental results showed that the optical loss of the sensor varies with temperature, and the sensitivity depends on the depth of the V-groove.

• 대한수의학회지
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• 제43권1호
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• pp.145-150
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• 2003

To determine effectively the chitinolytic activity of rCHT1 from the hard tick H. longicornis expressed in baculovirus-mediated Spodoptera frugtperda (Sf) 9 cells, a simple and sensitive assay system was established in solid phase using agarose gel containing ethylene glycol chitin as substrate. The various factors affecting the efficacy of the assay were also investigated. The effects of various temperature, dosages of proteins, pH of media and time courses of reaction were examined to verify the sensitivity of assay for chitinolytic activity of rCHT1 protein. It was found that the optimal reactive conditions were $37^{\circ}C$ of temperature, 12 to 15 hours of reactive times, $0.1{\mu}g$ of protein concentration and pH 5 to 7 of media. Using the assay system designed, the functional activities of H. longicornis rCHT1l protein could be evaluated simply and sensitively.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVII)
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• pp.711-712
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• 2005