• YAKHAK HOEJI
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• v.36 no.3
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• pp.205-211
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• 1992

A simple dignostic method using Fast Atom Bombardment mass spectrometry was applied to the characterization of green tea flavonols from the eluates of Sepahadex LH-20 column chromatography. From the ethyl acetate extracts, crude mixture of flavonol fraction(Fr.$1{\sim}4$) were separated by the stepwise gradient elution with 30, 45, and 60% aqueous acetone. Procyanidine B analogues were found to be typical constituents of Fr. 1. Main components of Fr. 2 were catechins and gallo-catechins. Fr. 3 contained mainly ester type compounds, catechin-gallates, gallocatechin-gallates with their analogues. Fr. 4 was contaminated with some phthalate esters.

• Journal of environmental and Sanitary engineering
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• v.21 no.3 s.61
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• pp.44-51
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• 2006

To estimate the inhibitory effect of Rubus coreanum extract on melanin biosynthesis, we tested its inhibitory effects on tyrosinase promoter in B16 mouse melanoma cells. Rubus coreanum extract by methanol had inhibitory effect approximately 10% on tyrosinase promoter at $100{\mu}g/mL$. In the MTT assay, the same extract exhibited very low cytotoxicity under $100{\mu}g/mL$. The fractions of methylene chloride, butyl alcohol and water did not showed the inhibitory effect on tyrosinase promoter, but the fraction of ethyl acetate exhibited inhibitory effect approximately 11% at $100{\mu}g/mL$.

• Korean Journal of Pharmacognosy
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• v.29 no.3
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• pp.179-186
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• 1998

The fractions of Uncariae Ramulus et Uncus seemed to be closely related with the levels of amino acids and other components which concerns with formation and metabolism of neurotransmitters in brain. The pretreatments of methanolic extract and its fractions prohibited the pentylenetetrazole (PTZ) induced convulsion. In such cases, lowered levels of ${\gamma}-aminobutyric$ acid and glutathione in brain were significantly recovered. And also the increased levels or activities of lipid peroxide, ${\gamma}-aminobutyric$ acid aminotransferase, xanthine oxidase, aldehyde oxidase, superoxide dismutase, catalase and glutathione peroxidase by PTZ-convulsion were lowered to normal state.

• Korean Journal of Pharmacognosy
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• v.29 no.3
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• pp.187-192
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• 1998

The ethyl acetate fraction of Uncariae Ramulus et Uncus, which showed anticonvulsant effects against pentylenetetrazole (PTZ) treated mice, were subjected to column chromatography to isolate ursolic acid and hyperin from active eluate. Hyperin decreased the elevated activities of GABA-T and xanthine oxidase and lipid peroxide level dose-dependently in PTZ treated mice brain tissue in vitro, but no effect on superoxide dismutase activity. The effects on such enzyme and component seemed to be related with biosynthesis or metabolism of neurotransmitters.

• Korean Journal of Pharmacognosy
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• v.28 no.3
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• pp.99-103
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• 1997

To develop anticariogenic agents, Scutellariae Radix was tested for its antibacterial activity against Streptococcus mutans OMZ176 which is known as a strong cariogenic bacterium. The antibacterial activity was evaluated with disk plate method and minimal inhibitory concentration (MIC). The ethyl acetate fraction showed significant activity. By means of activity-guided fractionation, baicalein and ganhuangenin were isolated as the active principles. The MICs of these compounds were $100{\mu}g/ml$ and $200{\mu}g/ml$, respectively.

• Natural Product Sciences
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• v.18 no.2
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• pp.137-140
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• 2012

The aim of this research was to investigate the industrial application of Zanthoxylum schinifolium. Antibacterial activities of the n-hexane, methylene chloride (MC), ethyl acetate, and butanol fractions of Z. schinifolium were tested against Escherichia coli, Staphylococcus aureus and Helicobacter pylori. Among the fractions, the n-hexane and MC fractions showed the stronger antibacterial activity against H. pylori, with an inhibition zone greater than 10 mm in disc assays. Further testing of bergapten and lupeol from the MC fraction of Z. schinifolium revealed the antibacterial effects against E. coli, S. aureus and H. pylori, suggesting their potential use as antibacterial agents.

• Natural Product Sciences
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• v.20 no.3
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• pp.206-210
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• 2014

Phytochemical constituents were isolated from Pinus koraiensis needles by open column chromatography. The structures isolated from the ethyl acetate fraction of P. koraiensis needles were elucidated as afzelin (1) and quercitrin (2) on the basis of spectroscopic analyses including $^1H$-NMR and MS. The amounts of afzelin (1) and quercitrin (2) in genus Pinus were determined by HPLC/UV analysis. Afzelin (1) and quercitrin (2) contents were highest in P. thunbergii needles. Because P. thunbergii needles contain high amounts of afzelin (1) and quercitrin (2), they have promising potential as new additives to natural medicinal products, health supplements, and beverages.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.186-186
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• 1998

Persicaria lapathifolia Gray (Polygonaceae) is a common weed in Korea. This plant and other Persicaria species including P. orientale and P. pubescens have been used as an analgesic and stomachic as well as for the treatment of rheumatoid arthritis and malaria. During the screening program of plant extracts, MeOH extract of P. scabrum showed anticomplement activity and the MeOH extract was partitioned with hexane, chloroform, ethyl acetate, and buthanol. EtOAc fraction showed strong activity and activity guided separation yielded eight flavonoids. Two known galloylated and a novel ferulloylated flavonoid glycosides showed strong anticomplement activity. Other flavonoid glycosides, kaempferol 3-O-${\alpha}$-$\sub$L/-arabinopyranoside, kaempferol 3-O-${\beta}$-$\sub$D/-glucopyranoside, kaempferol 3-O-${\beta}$-$\sub$D/-galactopyranoside, quercetin 3-O-${\alpha}$-$\sub$L/-arabinopyranoside, quercetin 3-O-${\beta}$-$\sub$D/-glucopyranoside, quercetin O-${\beta}$-$\sub$D/-galactopyranoside did not showed anicomplement activity.

• Mycobiology
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• v.34 no.3
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• pp.114-119
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• 2006

Biochemical variability among 20 Indian isolates of Sclerotinia sclerotiorum collected from different hosts/soil samples from different localities in India is reported. High Performance Liquid Chromatographic (HPLC) analysis of ethyl acetate fraction of culture filtrate, mycelia, sclerotia and sclerotial exudate showed $15{\sim}23$ peaks but only 11 could be identified. They were tannic, gallic, oxalic, caffeic, vanillic, ferulic, O-coumeric, chlorogenic, cinnamic, salicylic and gentisic acids. The amount of phenolic compounds varied among the culture filtrates, mycelia, sclerotia and sclerotial exudates of S. sclerotiorum.

• Advances in Traditional Medicine
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• v.4 no.1
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• pp.44-48
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• 2004

The Hexane Extract (HE) and Ethyl Acetate Soluble Fraction of the Methanolic Extract (EASFME) of the fruit pulp of Momordica dioica Roxb. (Cucurbitaceae) was evaluated for its anti-hepatotoxic activity in rats. Acute hepatotoxicity was induced by administering paracetamol (2 g/kg, p.o.) for 3 days. The extracts, at a dose of 400 mg/kg (p.o.) administered for 7 days exhibited a significant therapeutic effect by lowering Serum Glutamate Oxaloacetate Transaminase (SGOT), Serum Glutamate Pyruvate Transaminase (SGPT), Serum Alkaline Phosphatase (ALP) and Serum bilirubin and increasing the serum protein levels. These biochemical observations were supplemented by histopathological examination of the liver sections. The activity of extract was also comparable to the standard drug Silymarin, which is a well-known natural anti-hepatotoxic drug.