• Applied Biological Chemistry
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• v.18 no.4
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• pp.203-218
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• 1975

In order to investigate the compositional change oil composts during the growing of cultivated mushroom (Agaricus bisporus), composts and mushrooms during the period of filling to ending under commercial conditions were subjected to chemical analyses. The results are summarized as follows and the mechanism of composting for mushroom cultivation was proposed. 1) The temperature change of growing bed and room was observed and the yield of mushroom for each cropping time was recorded to get $15.6kg/m^2$ in total crops. 2) Composts after filling showed pH 8.2 which dropped to 6.4 after casing and continued so up to ending. 3) On the dry weight basis of composts, crude ash increased whereas total nitrogen, ether extract and crude fibre decreased gradually to bring about the lowering of organic matter. 4) Total nitrogen of composts decreased gradually and more insoluble nitrogen was lost than soluble nitrogen. The C/N ratio of composts was initially 21 which was gradually lowered to 16. 5) The losses of ${\alpha}-cellulose$, pentosan and lignin in composts were 87%, 75%, and 60%, respectively, in which ${\alpha}-cellulose$ decreased markedly after casing. 6) Free reducing sugars of composts increased continuously. Gradually increased free amino acids till second cropping decreased again thereafter. Composts at the filling stage contained alanine, glutamic acid, glycine and serine in which glycine decreased markedly whereas proline increased remarkably upon mushroom cultivation. 7) Among minerals of composts, phosphorus and zinc tended to decrease, potassium and copper tended to increase anti sodium showed no marked change. 8) In comparison of mushrooms from different cropping time with respect to proximate composition, minerals, free reducing sugars and amino acids, no marked difference was observed. However, a little higher values were observed in crude fat, free reducing sugars and sodium content for early crops and in free amino acids and phosphorus content for late crops. Twelve free amino acids including alanine, serine, threonine, and glutamic acid were detected in the cultivated mushroom. 9) According to above experimental results, it was possible to support the mechanism of compositing that the formation of ammonia and decomposition of carbohydrates by mesophiles are followed by protein biosynthesis, formation of microbial bodies and nitrogen-rich lignin humus complex by thermophiles, thus supplying necessary nutrients for mushroom growth, along with residual carbohydrates.

• Korean Journal of Poultry Science
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• v.18 no.2
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• pp.67-84
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• 1991

The purpose of this study was to investigate the effects of dietary protein levels on laying hen performance. The level of methionine and lysine were 0.32% and 0.64%, respectively and the levels of protein were 12%, 13%, 14% or 15%. Total 384 laying pullets of 22weeks age were reared from January 28, 1989 to March 23, 1990 for 60 weeks. The results obtained were summarized as follows : 1 Egg productions was highest at 15% of protein in phase I, 14% in phase II, and 13% in phase III, and there was significantly different egg Production among treatments during phase I and phase II (P＜0.05). 2. Egg weight was heaviest in 14% of protein treatment in three phases and they showed significantly different egg weight among different levels of protein in phase I (P＜0.01), phase II and III (P＜0.05) , but there was not significantly different between 14% and 15% of protein. 3. Daily egg mass tends to increase followed by increasing of protein level and showed signifiant differences among treatments in phase I and phase II (P＜0.01). 4. The 14% of protein treatment showed the highest daily feed intake and it showed significant difference in phase I and phase II (P＜0.01) , but there was no significant difference between 14% and 15% of protein. 5. Feed efficiency was improved significantly followed by increasing of protein level in phase I (P＜0.01) and phase II (P＜0.05), but there was no significant difference among treatments in phase III. 6. Viability tends to increase as increasing of protein level, but there was no significant difference among treatments. 7. Utilizabilities of dry matter, crude protein and ether extract of experimental diets were not different among treatments, but the utilizability of carbohydrate tends to increase as increasing of protein level (P＜0.05). 8. Eviscerated yield and abdominal fat accumulation was not difference among treatments. 9. Egg shell quality and chemical composition of egg content were not different among treatments. 10. The feed cost per kg egg mass showed the cheapest in 13% of protein treatment in all phase, but there were no significant differences among treatments.

• Journal of The Korean Society of Grassland and Forage Science
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• v.10 no.1
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• pp.42-47
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• 1990

Silage productivity of 7 corn and 3 sorghum hybrids was determined at two planting dates differing in rice black-streaked dwarf virus (RBSDV) infection rates in the southern part of Korea where RBSDV is prevalent. 1. Percent stand was similar between two planting dates, but that of corn hybrids (85.0-99.5 %) was higher compared with sorghum (59.8-82.3 %). Culm length of corn hybrids at April 23 planting was longer compared with May 20 planting, but that of sorghum hybrids was similar between two planting dates. 2. RBSDV infection rate of corn hybrids at April 23 planting was lower compared with May 20 planting, but sorghum was not infected at both planting dates. Among corn hybrids Suweon 19 was most severely infected, but Suweon 99 was least infected at both planting dates. 3. Percent ear bearing plants and earltotal dry matter (TDM) ratio of corn hybrids at April 23 planting was higher than those of May 20 planting. Among the corn hygrids percent ear beraring plants, ear/TDM ratio of Suweon 19 was lowest at both planting dates due to severe RBSDV infection. Ear/TDM ratio of sorghum hybrids was below 17.8 % due to sterility and partially bud damages. 4. Silage yield at April 23 planting was higher than that at May 20 planting and sorghum hybrids outyielded corn hybrids. Silage yield of Suweon 19 and Jinjuok was lowest due to severe RBSDV infection. 5. Crude protein was similar regardless planting dates, species, and varieties except the lowest P 931. Ether extract at April 23 planting was lower then that at May 20 and corn was lower compared with sorghum hybrids. Crude fiber and nitrogen free extracts(NFE) was similar between planting dates. Crude fiber of corn was lower, but NEF was higher compared with sorghum, but similar among hybrids in a species. 6. In vitro dry matter digestibility (IVDMD) at May 20 planting was higher than that of April 23 planting and corn was higher than sorghum hybrids. Digestible dry matter (DDM) of both corn and sorghum at April 23 planting was higher than that of May 20 planting. At April 23 planting DDM of all corn and sorghum hybrids was similar, but DDM of Suweon 19 and Jinjuok was lower than other corn and sorghum hybrids.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.307-318
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• 2005

This study was conducted to examine effects of brewery meal-based fermented feedstuff supplemented with Aspergillus oryzae(AO) or Saccharomyces cerevisiae(SC) on luminal micro-organism of Korean native cattle. Two cows equipped with luminal cannulas were used as experimental animals. Experiment was done with three treatment groups: $71.5\%$ of commercial feed and $28.5\%$ of com silage(control): $45.0\%$ of commercial feed, $26.5\%$ of fermented feedstuff supplemented with AO and $28.5\%$ of corn silage(TAO): $45.0\%$ of commercial feed, $26.5\%$ of fermented ffedstuff supplemented with SC and $28.5\%$ of corn silage(TSC). The number of total viable bacteria (p<0.05), anaerobic fungi and protozoa(p<0.05) was higher in TAO and TSC than in control. The number of proteolytic bacteria(p<0.05), cellulolytic bacteria and xylan fermenters tended to be higher in TAO and TSC than in control. The dry matter recovery (DMR) of protozoa was higher in TAO and TSC than in control(p<0.05). The crude protein (CP) content of total microbes and protozoa was higher in TSC than in control and TAO (p<0.05). The CP content of bacteria was higher in TAO and TSC than in control(p<0.05). The ether extract(EE) content of the total microbes was higher in TAO than in control and TSC(p<0.05), and the EE of protozoa and bacteria were higher in TSC than in control and TAO(p<0.05). The ratio of essential amino acids of total microbe was higher in control than in TAO and TSC(p<0.05). The ratio of methionine and alanine of bacteria was higher in TAO and TSC than in control(p<0.05). The results suggested that the feeding of fermented feedstuff supplemented with AO or SC had an influence on the numbers of ruminal microorganism and the changes of microbial body composition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.297-306
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• 2005

This study was conducted to compare and evaluate fermentable and nutritional characteristics of brewery meal-based fermented feedstuffs supplemented with Aspergillus oryzae (AO) and Saccharomyces cerevisiae (SC). Experiments were divided into three treatment groups; fermented feedstuff supplemented with $1\%$ of AO(FFAO), fermented feedstuff supplemented with $1\%$ of SC(FFSC), and fermented feedstuff supplemented with $0.5\%$ of AO and $0.5\%$ of SC(FFAS). For changes of crude protein contents by 48 h fermentation, there were no significant differences among treatments. Ether extract(EE) contents were significantly increased by 48 h fermentation (p<0.05). Neutral detergent fiber(NDF) contents of FFAO, FFSC and FFAS were significantly decreased by 48 h fermentation(p<0.05), but acid detergent fiber(ADF) and acid detergent lignin (ADL) contents were not different. The pH of FFAO and FFAS was decreased more rapidly than that of FFSC(p<0.05), reaching a plateau after 24 h. Alcohol content was increased rapidly until 18 h in FFAO and was increased rapidly until 12 h in FFSC and FFAS, and alcohol content of FFAO, FFSC and FFAS was maintained constantly after 24 h. The ammonia N content of FFAO, FFSC and FFAS was 0.022, 0.073 and $0.040\%$ at 48 h, respectively, and then ammonia N was over twice higher in FFSC than in FFAO and FFAS(p<0.05). Dextrose content was increased until 6 h in FFAO but was rapidly decreased in FFSC and FFAS until 6 h(p<0.05). Lactate content was higher in FFAO and FFAS than in FFSC(p<0.05). Consequently, when we added AO in formulation of fermented feedstuff with brewery meal which moisture content was high, EE, alcohol, and lactate contents were increased, but NDF and ammonia N contents were reduced. Therefore, it is expected that AO will be effective to increase the feed value and the preservation of fermented feedstuff with a high moisture content.

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.4
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• pp.345-354
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• 2009

The barley (Hordeum vulgare L.) is now widely grown as a whole crop silage in Korea, but the quality of that silage does not examined from farms. Therefore, this experiment was conducted to evaluate the forage quality of whole crop barley that was participated in Silage Quality Contest in 2008. These data were classified by region, dry matter (DM) yield, with or not additive, planting and harvest date. Difference on the lactic acid content of barley silage was detected in the region, DM yield, additive and harvest date (p<0.05), however, there were partially significant differences in chemical composition. There were significant differences among moisture content in DM yield, pH in additive, and crude ash content in additive and planting date of barley silage. Crude protein content was significant difference in the region, and ether extract content was in region, DM yield and harvest date, however, non-fiber carbohydrate was significant difference in planting date of barley silage. Neutral detergent fiber, acid detergent fiber and relative feed value were significant difference only in the cultivation region of whole crop barley. According to this experiment, differences in the silage quality were observed among whole crop barley silages. Therefore, nutritive value as well as moisture and pH are important in silage quality evaluation of whole crop barley. Especially, lactic acid is an important factor for the evaluation of whole crop barley silage.

• Applied Biological Chemistry
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• v.26 no.1
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• pp.8-18
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• 1983

The effects of fat-solvents was investigated on the yield. brown color intensity, UV absorbance patterns, reducing and antioxidant activities, and variation of fatty acid composition of the extracts from white and red ginseng. The yield and intensity of brown color of extracts were generally greater as the polarity of the solvent used became stronger. The intensity of the brown color of extract of red ginseng was greater than that of white ginseng. The orders of reducing and antioxidant activities of extracts of red ginseng was similar that of white ginseng, resulting in decreasing order of: ethanol>methanol>ethyl acetate, acetone>ether>chloroform>benzene, hexane. The ethanol, methanol, and ethyl acetate extracts of red ginseng showed stronger UV absorption than the corresponding extracts of white ginseng. The former also possessed stronger reducing and antioxidant activities than the latter. The composition of the major unsaturated fatty acids (linoleic, linolenic, and nervonic acid) in the ethanol and ethyl acetate extracts from both white and red ginseng did not change appreciably for 60 days at $45^{\circ}C$. In case of the hexane extracts which had shown the weakest reducing and antioxidant activities among the extracts, linolenic acid disappeared almost under the same condition.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.2
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• pp.124-128
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• 2016

The objective of this research was to determine the effects of harvesting frequency and fertilization levels on botanical composition, dry matter yield, and forage feed compositions of Alpine grassland at 800 m altitude. This research lasted for three years at National Alpine Agricultural Research Institute in Pyeongchang with two harvesting frequency schedules (two and three times annually) and two levels of fertilizer application (conventional level of fertilizer at 280-200-240 kg/ha and a lower level of fertilizer at 200-200-200 kg/ha for N, $P_2O_5$, and $K_2O$). Mixture combinations with seeding rate (kg/ha) were as follows: Orchardgrass 18, Tall fescue 9, Timothy 8, Kentucky bluegrass 3, and Ladino Clover 2. The gramineae ratio ranged from 93.2 to 95.3%. Therefore, gramineae forage was considered as the dominant plant in this experiment. No significant (p>0.05) difference was observed in forage dry matter yield between the two harvesting frequency treatments (two times at 9.8 ton/ha and three times at 8.6 ton/ha). However, forage dry matter yield in the two times of harvesting frequency tended to be greater than that in the three times of harvesting frequency. Significantly (p<0.05) higher forage dry matter yield in the standard fertilization level group than the lower fertilization level group (9.8 ton/ha vs. 8.7 ton/ha) was observed. However, there was no significant (p>0.05) difference in forage crude protein concentration between the two harvesting frequency treatment groups, although the concentration in the group with three times of harvesting frequency tended to be higher. In contrast, crude fiber concentration in the group with two times of harvesting frequency tended to be higher, although the difference was not statistically significant (p>0.05). Crude protein, ether extract, crude fiber, and organic matter concentrations were not significantly (p>0.05) different between the two groups with different fertilization levels. Based on these results, it was concluded that the group with two times of harvesting frequency with conventional fertilization level might be proper for obtaining better forage productivity for Alpine grassland at 800 m altitude.

• Journal of The Korean Society of Grassland and Forage Science
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• v.43 no.4
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• pp.268-273
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• 2023

The present study estimated rumen fermentation characteristics and greenhouse gas emissions of different forages. Alfalfa, timothy, tall fescue, Italian ryegrass, and rice straw as the main forage sources for Hanwoo were used in the present study. Crude protein was highest in alfalfa but lowest in rice straw (p<0.05). Ether extract was higher in alfalfa and Italian ryegrass than in the other forages (p<0.05). Crude ash was highest in rice straw but lowest in tall fescue (p<0.05). Neutral detergent fiber was highest in tall fescue but lowest in alfalfa (p<0.05). Acid detergent fiber was highest in Italian ryegrass and rice straw but lowest in alfalfa (p<0.05). In vitro digestibilities of dry matter (DMD) and neutral detergent fiber (NDFD) were highest in timothy but lowest in rice straw (p<0.05). Rumen pH was highest (p<0.05) in alfalfa, while ammonia-N was higher (p<0.05) in alfalfa and Italian ryegrass than in the other forages. Total volatile fatty acid was highest (p<0.05) in timothy, while acetate and propionate were highest (p<0.05) in alfalfa and rice straw, respectively. Acetate to propionate ratio was higher (p<0.05) in alfalfa, timothy, and Italian ryegrass than in rice straw. Rice straw had lowest total gas (mL) (p<0.05) but highest its per DMD and NDFD. Rice straw had higher (p<0.05) CO₂ (per DMD and NDFD) compared to alfalfa (per DMD and NDFD), timothy (per DMD and NDFD), tall fescue (per NDFD), and Italian ryegrass (per DMD). Again, rice straw had higher (p<0.05) CH₄ (per DMD and NDFD) compared to timothy (per DMD and NDFD) and tall fescue (per NDFD). Therefore, this study indicates that timothy has a higher nutrient digestibility and volatile fatty acid in the rumen leading to a reduction of greenhouse gas emission.

• Journal of The Korean Society of Grassland and Forage Science
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• v.36 no.4
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• pp.344-349
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• 2016

This study was conducted to evaluate the possibility of expanding the usage of whole crop silage from beef cattle and dairy cow to hogs and chickens. For this purpose, a crushing device was developed to crush whole crop silage. The crushed silage was sealed, and analyzed for its feed value. The silage varieties used for the experiment included Saessal barley and Geumgang wheat. Whole crop barley and wheat were crushed in the crushing system as a whole without separating stems, leaves, grains, etc.. When the crushed whole crop silages (CWCS) were analyzed, full grain, grains above 10 mm in size, grains 5~10 mm in size, and grains below 5 mm in size accounted for, 20%, 4%, 27%, and 49 %, respectively. In order to facilitate the fermentation of CWCS, inoculated some fermenter into each CWCS sample (barley or wheat). As control, another set of sample was not inoculated. Crude protein (CP), ether extract (EE), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin, cellulose content, total digestible nutrient (TDN), and relative feed value (RFV) of fermenter-inoculated Saessal barley were 2.45 %, 1.61%, 8.95%, 16.94%, 9.52%, 1.01%, 8.51%, 81.38%, and 447.5%, respectively. The CP, EE, CF, NDF, ADF, lignin, cellulose content, TDN, and RFV in the other sample of Saessal barley without inoculation of fermenter were 2.57%, 1.62%, 9.61%, 18.25%, 10.13%, 1.10%, 9.04%, 80.90%, and 412.9%, respectively. The CP, EE, CF, NDF, ADF, lignin, cellulose content, TDN, and RFV of fermenter-inoculated Geumgang wheat sample were 2.43%, 1.27%, 10.99%, 19.49%, 11.23%, 1.46%, 9.77%, 80.03%, and 382.6%, respectively. The CP, EE, CF, NDF, ADF, lignin, cellulose content, TDN, RFV of the other set sample of Geumgang wheat sample without the inoculation of fermenter were 2.28%, 1.44%, 10.08%, 18.02%, 10.44%, 1.26%, 9.18%, 80.65%, and 416.9%, respectively. The TDN and RFV content in the fermenter-inoculated Saessal barley were 81.38% and 447.5%, respectively, while the one in the fermenter-inoculated Geumgang wheat were 80.03% and 382.6% respectively. When the feed value of whole crop barley and wheat silage without crushing process was compared to the feed value of whole crop barley and wheat silage made from crushing system, the latter appeared to be higher than the former. This could be due to the process of sealing the crushed silage which might have minimized air content between samples and shortened the golden period of fermentation. In conclusion, these results indicate that a crushing process might be needed to facilitate fermentation and improve the quality of silage when making whole crop silage.