• Applied Biological Chemistry
• /
• v.34 no.1
• /
• pp.61-66
• /
• 1991

In order to improve the productivity of ethanol by sugar-alcohol-tolerant Saccharomyces cerevisiae D1, the effect of addition of soybean meal on the alcohol fermentation was investigated. The addition of soybean meal led tn the increase of the ethanol productivity and viable cell concentration. Increasing the mont of soybean meal increased the number of viable cells and the consumption percentage of glucose. The water-soluble fraction of soybean meal was nearly as effective as whole-soybean meal, whereas the lipidic fraction had no positive effect. The addition of 4% soybean meal increased the rate of ethanol production regardless of the initial concentrations of glucose. The rate of glucose consumption fermenting a soybean meal supplemented medium was higher than possible in a non-supplemented medium, either in the absence or in the presence of ethanol. But the percentage of ethanol inhibition of the glucose consumption rate was identical for supplemented md unsupplemented media. The increase of final ethanol concentration could not be attributed In an increase of ethanol tolerance of yeast cells but to the satisfaction of nutritional deficiencies.

• Journal of Microbiology
• /
• v.33 no.4
• /
• pp.322-327
• /
• 1995

Tolerance of Mycobacterium sp. against organic solvents has been induced for the cholesterol side chain degradation by adding chemicals associated with synthesis of fatty acids or alcohols. Biotin of 300 .mu.g/1 and 0.5% aqueous ethanol solution were optima for the enhancement of ethanol tolerance of the microorganism. The induction of ethanol tolerance by biotin was found to be due to increase of degree of unsaturation of the fatty acids in membranous phospholipid of the cell, especially due to increase of oleic acid content. However when 0.5% of ethanol was added for the ethanol tolerance induction, there was an ambiguous correlation between ethanol tolerance and degree of unsaturation of the fatty acids, in spite of the fact that the induction increased the content of unsaturated fatty acids. Addition of 0.5% of ethanol induced several ethanol shock proteins having molecular weight similar to that of heat shock proteins.

• KSBB Journal
• /
• v.18 no.2
• /
• pp.127-132
• /
• 2003

The effect of 4 kinds of alcohols was investigated on the production of bacterial cellulose (BC) by Gluconacetobacter hansenii PJK. The addition of alcohols and acetic acid to medium caused the pellets of bacterial cellulose to aggregate into a lump, which could be easily separated from the culture medium. The growth rate of cells and the production yield of BC increased in the medium containing ethanol. Other alcohols in the medium decreased cell growth and the cellulose production rate, because of their toxic effects. The addition of ethanol depressed the conversion of a $\textrm{Cel}^{+}$ cell to a $\textrm{Cel}^{-}$ mutant in shaking culture. Cells subcultured three in a medium containing ethanol produced BC without any loss of BC production yield.

• Archives of Pharmacal Research
• /
• v.20 no.5
• /
• pp.432-437
• /
• 1997

We used a herbal medicine, roots of Rhodiola sachalinensis (RS) to assess whether RS extract can decrease blood ethanol concentrations in rats fed ethanol and if so, to elucidate the mechanism by which RS extract reduces blood ethanol levels. Rats were fed ethanol orally 1 hr after the oral administration of various doses of RS extract. In another experiment, rats were injected intraperitoneally with ethanol following the intake of RS extract via gastric catheter to eliminate possible inhibition of ethanol absorption in the gastrointestine by RS extract. The administration of RS extract remarkably lowered blood ethanol levels in a dose-dependent manner in rats given ethanol orally. However, the intake of RS extract did not reduce ethanol levels in rats injected with ethanol intraperitoneally. The activities of two main hepatic enzymes, alcohol dehydrogenase (ADH) and aldehyde dehydrogenase (ALDH), involved in ethanol metabolism, were not affected by the administration of RS extract in rats fed ethanol. In addition, the intake of RS extract reduced serum triglyceride levels elevated by ethanol to the normal level. We conclude that the administration of RS extract lowers blood ethanol concentrations by inhibition of ethanol absorption in the gastrointestinal tracts of ethanol-fed animals.

• Corrosion Science and Technology
• /
• v.9 no.2
• /
• pp.92-97
• /
• 2010

Many kinds of alternative fuels such as biodiesel, ethanol, methanol, and natural gas have been developed in order to overcome the limited deposits in fossil fuels. In some cases, the alternative fuels have been reported to cause degrade materials. The corrosion rates of metals were measured by immersion test, a kind of time consuming test because low conductivity of these fuels was not allowed to employ electrochemical tests. With twin two-electrode cell newly designed for the study, however, electrochemical impedance spectroscopy (EIS) test was successfully applied to evaluation of the corrosion resistance ($R_p$) of zinc, iron, aluminum, and its alloys in an oxidized biodiesel and gasoline/ethanol solutions and the corrosion resistance from EIS was compared with the corrosion rate from immersion test. In biodiesel, $R_p$ increased in the order of zinc, iron, and aluminum, which agreed with the corrosion resistance measured from immersion test. In addition, on aluminum showing the best corrosion resistance ($R_p$), the effect of magnesium as an alloying element was evaluated in gasoline/ethanol solutions as well as the oxidized biodiesel. $R_p$ increased with addition of magnesium in gasoline/ethanol solutions containing chloride and the oxidized biodiesel. In the mean while, in gasoline/ethanol solutions containing formic acid, Al-Mg alloy added 1% magnesium had the highest $R_p$ and the further addition of magnesium decreased $R_p$. It can be explained with the fact that the addition of more than 1% magnesium increases the passive current density of Al-Mg alloys.

• Asian-Australasian Journal of Animal Sciences
• /
• v.5 no.3
• /
• pp.511-517
• /
• 1992

Ethanol was utilized by mixed rumen microbes, but addition of pentachlorophenol (25 mg/l), a methanogen inhibitor, suppressed the utilization of ethanol. Carbon monoxide (50% of the gas phase), a hydrogenase inhibitor, more strongly suppressed the utilization of ethanol, propanol, and butanol. These results suggest that the major ethanol utilizers are $H_2$ producers. Ethanol utilization was depressed at low pH (below 6.0). Since methanogens were shown to be relatively resistant to low pH, it appears that ethanol utilizers are particularly sensitive to low pH. Ruminococcus albus and R. flavefaciens in mono-culture produced ethanol from carbohydrate (glucose and cellobiose), even when a high level (170 mM) of ethanol was present. Ethanol was not utilized even in the absence of carbohydrate, but the co-culture of these bacteria with methanogens resulted in the utilization of ethanol, i.e., when $H_2$ was rapidly converted to $CH_4$, R. albus and R. flavefaciens utilized ethanol. These results suggest that ethanol is utilized when the electrons liberated by the oxidation of ethanol are rapidly removed, and ready electron disposal in ethanol-utilizing, $H_2$-producing bacteria is accomplished by the interspecies transfer of $H_2$.

• Korean Journal of Medicinal Crop Science
• /
• v.23 no.3
• /
• pp.231-236
• /
• 2015

Skin anti-wrinkle activities of the stems and leaves of Abeliophyllum distichum Nakai were evaluated by the extracts obtained from various extraction processes such as using hot water at $100^{\circ}C$, 70% ethanol at $85^{\circ}C$, and 70% ethanol with ultrasonication at $60^{\circ}C$ The ultrasonicated extract showed 95.62% of the highest cell viability in addition of $0.3mg/m{\ell}$ of the extracts into the normal human fibroblast cell, CCD-986sk. For antioxidant activities, the extracts using ultrasonicated extract showed the highest DPPH free radical scavenging as 80.27%, followed by 75.88% and 62.44% for the extracts using ethanol extract and water extract. The ultrasonicated extract also showed the highest elastase inhibition activity as 25.32%, compared to ethanol extract and water extract based method at 22.01% and 12.88%, respectively. MMP-1 production was most effectively decreased down to $2908.1pg/m{\ell}$ with ultrasonicated extract while $6640.8pg/m{\ell}$ with water extract and $3609.3pg/m{\ell}$ with ethanol extract, in addition of $0.3mg/m{\ell}$. Collagen production was increased up to $154.7ng/m{\ell}$ in addition of ultrasonicated extract, and followed by $121.4ng/m{\ell}$ and $31.2ng/m{\ell}$ for ethanol extract and water extract, respectively. These results indicate that the ethanol extract should have skin anti-wrinkling activities and can be improved by the ultrasonication process that high energy input elute more amounts of bioactive substances eluting more amounts of bioactive substances from the high energy input of ultrasonication.

• YAKHAK HOEJI
• /
• v.58 no.5
• /
• pp.300-306
• /
• 2014

Ethanol consumption causes psychomotor alterations. Hovenia Semen seu Fructus (HS), widely distributed in Korea, China, and Japan, has been reported to have beneficial effects on acute alcohol-induced liver injury. The present study sought to assess the effects of HS extract on ethanol-induced psychomotor alterations in rats. Sprague-Dawley rats were orally (p.o.) given ethanol (4 g/kg) (ethanol group) to induce psychomotor alterations. A separate group (HS-treated groups), were treated with different dosages of HS (50, 100, and 200 mg/kg, p.o.), 30 minutes before ethanol treatment. The control group received only the vehicle (saline). Ethanol-induced psychomotor alterations were evaluated in the open-field, rota-rod, hanging wire, and cold swimming test. In addition, blood ethanol and acetaldehyde concentrations were also measured. Behavioral evaluations and blood analysis were carried out 0.5, 1, 2, 4, and 8 hours after ethanol administration. Pre-treatment of HS ameliorated ethanol-induced alterations in the open-field, rota-rod, and cold swimming test, significantly evident in 2 and 4 hours after ethanol treatment. These improvements coincided with decrease in blood ethanol and acetaldehyde concentration. Based on these results, the present study suggests that HS may have ameliorating effects against ethanol-induced psychomotor alterations.

• Proceedings of the Korean Institute of Surface Engineering Conference
• /
• 2015.11a
• /
• pp.189-189
• /
• 2015

In this study, the corrosion resistance and adhesion of electrophoretic paint (E-paint) were studied on AZ31 magnesium alloy pretreated in cerium chemical conversion coating solutions with the addition of various ethanol concentrations. It was found that with increasing ethanol concentration from 0 to 90 percent can decrease the formation of $Mg(OH)_2/MgO$ and increase the formation of nano-crystalline cerium oxides on the coating. Both corrosion resistance and adhesion of E-painted AZ31 increased with increasing ethanol concentration. The best E-paint sample was observed on the sample pretreatment in cerium chemical conversion coating solution with the addition of 80 percent of ethanol. This sample showed an excellent adhesion without paint detached after water immersion test for 500 h at $40^{\circ}C$, and only a few blisters observed at the near scratched sites after 1000 h salt-spray test.

• KSBB Journal
• /
• v.23 no.6
• /
• pp.504-508
• /
• 2008

The objectives of this study were to develope the economical process for bioethanol production from domestic triticale and investigate optimal fermentation conditions such as temperature, time, and enzyme concentration used to pre-treatment process. Triticale mash, containing 148 g of total sugar per 1 L of mash, was fermented with Saccharomyces cerevisiae CHY1011 at $33^{\circ}C$. Fermentation of mash supplemented with enzyme was completed within 48-60 hours, and the ethanol yield was 410.9 L/tonne of dry base. On the other hand, fermentation of mash without enzyme addition was completed within 36-48 hours, but the ethanol yield was 342.2 L/tonne of dry base. For optimal bioethanol production from triticale, viscosity reduction enzyme was added in the pre-treatment process, and the fermentation rate of triticale was 92.0-94.2%. In addition, the results showed that bioethanol production of triticale by low-temperature pre-treatment would provide higher ethanol production efficiency and lower operating costs.