• 한국식품영양학회지
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• 제12권1호
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• pp.33-38
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• 1999

This study was conducted to investigate the synergic effect of dietary selenium and methionine levels on hepatic lipid metabolism in ethanol treated rats. Sprague-Dawley male rats were fed diets containing three levels of methionine(0,3 and 9g/kg diet) with or without selenium(0.45mg/kg diet). Ethanol was administered with 25%(v/v) ethanol orally at the same time once a day in ethanol group and isocalori sucrose was administered to the control group. The rate were sacrificed after 5 and 10 weeks of feeding period. Glutathione content was decreased by ethanol treatment and significantly increased in proportion to level of dietary methionine and was higher in selenium deficiency group than that of selenium admin-istration group. Lipid peroxide content was significantly increased in deficiency of both methionine and selenium(LMet-Se+EtOH) group. Total lipid triglyceride and cholesteol contents in liver were increas-ed and phospholipid content was decreased in ethanol treated group and ethanol treatment accelerated those increment and decrement in methionine deficiency(LMt) group and excessive methionine admin-istration(HMet)group.

• KSBB Journal
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• 제8권5호
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• pp.446-451
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• 1993

에탄올 발효에서의 주요 환경 인자인 온도, pH, 통 기속도, 초기 포도당 농도, 효모추출물 농도 등이 세 포성장과 에탄올 및 부산물 생성에 미치는 영향에 대하여 연구하였다. 통기속도가 증가함에 따라 최대 세포농도는 거의 션형적으로 증가하는 반면 최대 에 탄올 및 부산물 농도는 감소하였다. 배양온도가 높아질수록 세포의 성장속도와 에탄올 빛 부산물 생성 속도는 증가하는 반면 최종 세포농도와 최종 에탄올 및 부산물 농도는 오히려 낮게 나타난다. 배지내의 pH 벙위가 4.0 이하냐 6.0 이상으로 되연 세포성장, 에탄올 및 부산물 생성이 급격히 감소하였고 pH 4.5 에서 세포농도와 에탄올 및 부산물 농도가 최대치를 나타내었다. 초기 포도당 농도의 증가에 따라 세포 농도와 에탄올 및 발효 부산물 농도는 증가하였으나 수율은 오히려 감소하였다. 효모추출물 농도가 증가 하면 세포성장은 증가하나 에탄올 및 부산물의 생성 은 오히려 감소하였다.

• 펄프종이기술
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• 제47권1호
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• pp.17-23
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• 2015

In bioethanol from acid hydrolysis process, neutralization of acid hydrolyzate is essential step, which resulted in dissolved cations in glucose solution. Impact of cations to Saccharomyces cerevisiae in glucose solution was investigated focused on ethanol fermentation. Both potassium and sodium cations decreased the ethanol fermentation and glucose to ethanol conversion as potassium or sodium cations. In sodium cation, more than 1.13 N sodium cation in glucose solution led to ethanol production less than theoretical yield with severe inhibition. In 1.13 N sodium cation concentration, ethanol fermentation was slowed down to reach the maximum ethanol concentration with 48 h fermentation compared with 24 h fermentation in control (no sodium cation in glucose solution). In case of potassium cation, three different levels of potassium led to silimar ethanol concentration even though slight slow down of ethanol fermentation with increasing potassium cation concentration at 12 h fermentation. Sodium cation showed more inhibition than potassium cation as ethanol concentration and glucose consumption by Saccharomyces cerevisiae.

• 한국미생물·생명공학회지
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• 제10권2호
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• pp.155-162
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• 1982

당밀로부터 ethanol을 생산하기 위한 균체순환식 연속발효를 실시하였다. 균주는 Saccharomyces uvarum ATCC 26602를 사용 하였으며 발효온도는 35$^{\circ}C$였다. 발효중 ethanol에 의한 저해를 줄이기 위하여 이단계발효를 시행하였는데, 첫번째 단계에서 공기는 0.12vvm으로 공급하였고 두번째 단계에서는 혐기적상태로 발효를 진행하였다. 당농도를 14%로 희석했을 때 다른 무기물을 추가하지 않아도 ethanol 발효가 진행되었으며 단지 균체증식이 목적일 때는 phosphorus 첨가가 필요하였다. 균체순환식 연속발효로 14%의 당을 함유한 당밀희석액을 발효시키는데 14.5 시간이 소요되었다. 이때의 최종 ethanol 농도는 8.4~9.0%(v/v)로서 ethanol 생산비율은 이론식의 88.1~94.4% 이었다.

• 한국미생물·생명공학회지
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• 제7권2호
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• pp.91-95
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• 1979

Cellulose 및 hemicellulose 를 함유하는 볏짚 등 농산폐자원을 Trichoderma로 만든 Koui와 효모를 동시에 처리하여 단일공정으로 ethanol과 xylose 를 생산할 수 있었다. 볏짚 50g을 처리하여 ethanol 18mι와 xylose 2.7g 을 생산할 수 있었으며 옥수수속대 50g에서 ethanol 3.8mι와 xylose 10.8 g을 얻을 수 있었다. 이 처리에서 토양 분리균 Tri-choderma sp. KI 7-2로 만든 Koji가 T. reesei 의 그것보다 높은 효모활성을 보였다. 볏짚의 alkali 전처리는 ethanol 및 xylose의 생산에 큰 효과가 없었으며 이에 대하여 논하였다

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1987년도 Proceedings of Korea-Japan Panax Ginseng Symposium 1987 Seoul Korea
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• pp.47-58
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• 1987

Ethanol exerts different effects on hepatic cellular metabolism, depending mainly on the duration of its intake. In the presence of ethanol following an acute load, a number of hepatic functions are inhibited, including lipid oxidation and microsomal drug metabolism. In its early stages, chronic ethanol consumption produces adaptive metabolic changes in the endoplasmic reticulum which result in increased metabolism of ethanol and drugs and accelerated lipoprotein production. Prolongation of ethanol intake may result in injurious hepatic lesions such as alcoholic hepatitis and cirrhosis A number of such metabolic effects of ethanol are directly linked to the two major products of its oxidation; hydrogen and acetaldehyde. The excess hydrogen from ethanol unbalances the liver cell's chemistry. In the presence of excess hydrogen ions the process is turned in a different direction. In this study, it was attempted to observe the effect of ginseng saponins on alcohol Oehydrogenase(ADH), aldehyde dehydrogenase(ALDH) and microsomal ethanol oxidizing system(MEOS) in vivo as well as in vitro. Furthermore, the effect of ginseng saponin on the hydrogen balance in the liver and the hepatic cellular distribution of (1-14C) ethanol, its incorporation into acetaldehyde and lipids was also investigated. It seemed that ginseng saponin stimulated the above enzymes and other related enzymes in ethanol metabolism, resulting in a rapid removal of acetaldehyde and excess hydrogen from the animal body,

• 한국식품영양과학회지
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• 제19권1호
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• pp.1-12
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• 1990

To investigate effects of ethanol and dietary fat on growth and bichemical indices of liver tissue and blood in rats 40 male rats of Sprague-Dawley wtrain weighing about 160g were divided into 5 groups (low-fat diet group ethanol-administered low-fat diet group high-fat diet group ethanol-administered high-fat diet group and commercial diet group) and fed expe-rimental diets for 8 weeks. Ethanol-administered groups consumed ethanol corresponding to 22 cal% which was considered as moderate drinking. Neither the ethanol intake nor the dietary fat level affected calorie intake. Nonetheless the low-fat diet group with ethanol had the lowest growth rate and 2-fold increase in the concentration of plasma triglyceride. There was no effect of ethanol and dietary fat level on contents of protein lipid and lipid composition of liver tissue. The level of lipid peroxide of liver tissue tended to be increased by ethanol intake but the increase was statistically insignificatnt. The low-fat ethanol group had lowered hepatic mitochondrial respiration rate and deformed structure of mitochondria of hepatocytes.

• KSBB Journal
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• 제25권6호
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• pp.565-571
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• 2010

To develop thermostable ethanol fermentative yeast strain for lignocellulosic simultaneous saccharification and fermentation, high ethanol producing yeast, Saccharomyces cerevisiae CHY1012 and thermostable yeast, Kluyveromyces marxianus CHY1703 were fused by protoplast fusion. The thermostable fusant, CHY1612 was identified as a Kluyveromyces marxianus by phenotypic and physiological characteristics, as well as molecular analysis based on the D1/D2 domains of the large subunit (26S) rDNA gene and the internally transcribed spacer (ITS) 1 + 2 regions. For lignocellulosic ethanol production, AFEX pretreated barley straw at $150^{\circ}C$ for 90 min was used in a simultaneous saccharification and fermentation (SSF) process using thermotolerant CHY1612. The SSF from 16% pretreated barley straw at $43^{\circ}C$ gave a saccharification ratio of 90.5%, a final ethanol concentration of 38.5 g/L, and a theoretical yield of 91.2%. These results show that K. marxianus CHY1612 has potential for lignocellulosic ethanol production through simultaneous saccharification and fermentation with further development of process.

• KSBB Journal
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• 제26권5호
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• pp.417-421
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• 2011

Because beverage waste contains a lot of sugar, it can be used as a valuable resource for energy. But beverage waste is discharged through the water treatment. To prevent the waste of the energy resource, we produced bioethanol by using beverage waste in this study. In order to produce bioethanol, we added distillers stillage and NaOH for fermentation condition (nutrients and pH adjustment). As a results, ethanol concentration was 5.92 vol%. In contrast, ethanol concentration of blank (not added nutrients) was low and fermentation rate was very slow. Because components of the distillers stillage help the yeast growth, fermentation yield and rate was improved. Finally, we operated distillation and dehydration process by using fermented mash and produced fuel bioethanol (more than 99.5 wt%). We think that this results may provide useful information with application of commercial ethanol production using beverage waste.

• The Korean Journal of Physiology
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• 제7권2호
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• pp.25-30
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• 1973

In 30 rats divided into salt, ethanol and salt plus ethanol groups, the effect of ethanol on the course of hypertension induction with the salt ingestion was studied. The results obtained from the present study are as follows. 1) In salt group mean arterial blood pressure elevated to plateau (about 140 mmHg) in two weeks and the increased blood pressure was well maintained throughout entire experimental period. 2) By four weeks after ethanol ingestion, mean arterial blood pressure of ethanol group was slightly decreased followed thereafter by slow restoration to control value. And it was believed that decline of blood pressure observed in this case probably was not resulted from cardiac depression. 3) As mean arterial pressure in salt plus ethanol group remained rather low compared with that of salt group, it was suggested that ethanol may have a dose reduction effect in the course of hypertension induction by excessive salt ingestion. It was, however, not possible from the result of present study to decide that low blood Pressure in this group was resulted whether from enhanced sodium excretion activity of ethanol or from effect on blood pressure of ethanol itself.